Research in Biotechnology and Environmental Science,
Год журнала:
2024,
Номер
3(2), С. 18 - 22
Опубликована: Июнь 25, 2024
Introduction:
Microbes
of
sponges
have
diverse
associations,
including
true
symbiosis.
Sponges,
being
evolutionarily
ancient
sessile
filter
feeders,
host
and
abundant
microbial
species
that
play
crucial
roles
in
metabolism.
Although
the
symbionts
are
widely
distributed
within
organism
(up
to
40%
their
volume),
ecological
relationships
interactions
between
bacteria
sponge
remain
largely
unexplored
for
many
species.
The
present
study
was
one
first
attempts
isolate
symbiotic
from
Raspaciona
aculeata.
Materials
Methods:
After
isolation
on
marine
agar
medium,
isolates
were
characterized
different
colony
morphology.
16S
rDNA
taxonomic
analysis
carried
out
isolates.
Results:
Following
an
incubation
period
two
weeks
at
25°C,
only
13
bacterial
strains
isolated
with
a
very
low
rate
genetic
biodiversity.
All
belonged
Gammaproteobacteria
class
(Pseudomonadaceae
family),
except
(isolate
AL-18ra)
belonging
Bacilli
(Bacillaceae
family).
Conclusion:
obtained
results
great
importance
advancing
understanding
symbiosis
phenomena
aculeata
its
bioapplication
potential.
Abstract
The
capacity
of
marine
sponges
to
cope
with
environmental
changes
is
often
attributed
the
specific
composition
their
bacterial
communities.
In
this
study,
we
assessed
microbiome
two
dominant
sponges,
Rhabdastrella
globostellata
(Rg)
and
Hyrtios
erectus
(He),
living
in
Bouraké
lagoon
(New
Caledonia),
where
abiotic
conditions
daily
fluctuate
according
tide.
Sponge
specimens,
sediment
seawater
samples
were
collected
at
2–3
m
depth.
communities
using
16S
rRNA
metabarcoding,
variations
between
compared
PCA
biplots.
Chloroflexi
was
phyla
both
He
Rg
an
average
relative
abundance
41.2%
53.2%,
respectively,
while
it
absent
seawater.
Among
phylum
Chloroflexi,
SAR202
clade
reaching
53.2%
(He)
78.7%
(Rg).
Principal
component
analysis
(PCA)
used
identify
main
variables
driving
community
structure
sponges.
results
indicated
that
strongly
associated
(70.9%
variance)
(86.6%
variance).
high
observed
study
highest
reported
so
far
literature
Such
a
these
bacteria
could
suggest
involvement
well‐being
extreme
Bouraké.
Marine Drugs,
Год журнала:
2019,
Номер
17(10), С. 578 - 578
Опубликована: Окт. 11, 2019
Sponge-associated
bacteria
have
been
mostly
cultured
from
shallow
water
(≤30
m)
sponges,
whereas
only
few
studies
targeted
specimens
below
30
m.
This
study
assessed
the
cultivability
of
two
marine
sponges
Xestospongia
muta
and
Agelas
sventres
collected
(<30
m),
upper
mesophotic
(30–60
lower
(60–90
reefs.
were
cultivated
on
six
different
media,
replicate
plates
used
to
pick
individual
colonies
or
recover
entire
biomass.
Prokaryotic
community
analysis
was
conducted
using
Illumina
MiSeq
sequencing
16S
rRNA
gene
amplicons.
A
total
144
bacterial
isolates
picked
following
a
colony
morphology
coding
scheme
subsequently
identified
by
sequence
analysis.
Sponge
individuals
at
each
depth-range
harboured
specific
cultivable
that
not
retrieved
other
depths.
However,
there
substantial
differences
in
number
obtained
for
same
species.
In
addition,
source
inoculum
cultivation
medium
had
more
impact
prokaryotic
than
sample
collection
depth.
suggests
“plate
count
anomaly”
is
larger
sponge-associated
composition
related
Microorganisms,
Год журнала:
2020,
Номер
8(12), С. 1978 - 1978
Опубликована: Дек. 12, 2020
Marine
sponges
are
excellent
examples
of
invertebrate–microbe
symbioses.
In
this
holobiont,
the
partnership
has
elegantly
evolved
by
either
transmitting
key
microbial
associates
through
host
germline
and/or
capturing
microorganisms
from
surrounding
seawater.
We
report
here
on
prokaryotic
microbiota
during
different
developmental
stages
Plakina
cyanorosea
and
their
environmental
samples
a
16S
rRNA
metabarcoding
approach.
comparison
with
source
adults,
larvae
housed
slightly
richer
more
diverse
communities,
which
structurally
related
to
microbiota.
addition
thaumarchaeal
Nitrosopumilus,
parental
were
broadly
dominated
Alpha-
Gamma-proteobacteria,
while
offspring
particularly
enriched
in
Vibrionales,
Alteromonodales,
Enterobacterales
orders
Clostridia
Bacteroidia
classes.
An
enterobacterial
operational
taxonomic
unit
(OTU)
was
dominant
member
strict
core
The
most
abundant
unique
OTUs
not
significantly
amongst
microbiomes
specimens
included
sponge
microbiome
project.
wider
context,
Oscarella
genera
higher
divergence
associated
compared
Homoscleromorpha
counterparts.
Our
results
indicate
that
P.
is
low
abundance
(LMA),
appears
heavily
depend
horizontal
transmission
its
partners
likely
help
adaptation
habitat.
Antonie van Leeuwenhoek,
Год журнала:
2022,
Номер
115(10), С. 1265 - 1283
Опубликована: Авг. 23, 2022
In
this
study,
we
used
16S
rRNA
gene
amplicon
sequencing
to
investigate
prokaryotic
community
composition
of
the
Caribbean
sponges
Xestospongia
muta
and
Agelas
sventres
from
three
depth
ranges:
<
30
m
(shallow),
30-60
(upper
mesophotic),
60-90
(lower
mesophotic).
The
in
shallow
samples
X.
was
enriched
Cyanobacteria,
Chloroflexota,
Crenarchaeota
compared
mesophotic
depths,
while
were
Acidobacteriota.
For
A.
sventres,
relative
abundance
Acidobacteriota,
Gammaproteobacteria
higher
samples,
Proteobacteria
samples.
Antimicrobial
activity
evaluated
by
screening
crude
extracts
against
a
set
Gram-positive
Gram-negative
bacteria,
yeast,
an
oomycete.
Antibacterial
activities
sponge
individuals
generally
than
observed
individuals,
that
showed
limited
or
no
antibacterial
activities.
Conversely,
highest
anti-oomycete
found
lower
depth,
but
without
clear
pattern
across
gradient.
These
results
indicate
sponge-associated
communities
antimicrobial
change
within
species
gradient
depth.
Marine Drugs,
Год журнала:
2022,
Номер
21(1), С. 29 - 29
Опубликована: Дек. 29, 2022
Recent
reviews
have
reinforced
sponge-associated
bacteria
as
a
valuable
source
of
structurally
diverse
secondary
metabolites
with
potent
biological
properties,
which
makes
these
microbial
communities
promising
sources
new
drug
candidates.
However,
the
overall
diversity
metabolite
biosynthetic
potential
present
in
is
difficult
to
access
due
fact
that
majority
are
not
readily
cultured
laboratory.
Thus,
use
cultivation-independent
approaches
may
allow
accessing
“silent”
and
“cryptic”
gene
clusters
cannot
yet
be
cultured.
In
study,
we
investigated
(BGCs)
metagenomes
bacterial
associated
three
sponge
species:
Clathria
reinwardti,
Rhabdastrella
globostellata,
Spheciospongia
sp.
The
results
reveal
contain
high
number
predicted
BGCs,
ranging
from
282
463
BGCs
per
metagenome.
types
were
represented
12
different
cluster
types.
Clusters
encode
fatty
acid
synthases
polyketide
(PKS)
most
dominant
BGC
types,
followed
by
encoding
synthesis
terpenes
bacteriocins.
Based
on
sequence
similarity
analysis,
363
families
(GCFs)
identified.
Interestingly,
no
GCFs
assigned
pathways
responsible
for
production
known
compounds,
implying
detected
might
several
novel
compounds.
KS
sequences
PKS
used
predict
taxonomic
origin
involved.
related
phyla
Actinobacteria,
Proteobacteria,
Firmicutes
predominant.
At
genus
level,
KSs
those
found
genera
Mycolicibacterium,
Mycobacterium,
Burkholderia,
Streptomyces.
Phylogenetic
analysis
resulted
detection
two
‘sponge-specific’
i.e.,
SupA
SwfA,
well
phylum
Candidatus
Poribacteria
composed
only
assessed
here.
Marine Drugs,
Год журнала:
2022,
Номер
20(3), С. 190 - 190
Опубликована: Март 4, 2022
Species
misidentification
in
the
field
of
natural
products
is
an
acknowledged
problem.
These
errors
are
especially
widespread
sponge
studies,
albeit
rarely
assessed
and
documented.
As
a
case
study,
we
aim
to
revisit
reports
isomalabaricane
triterpenes,
isolated
from
four
demosponge
genera:
Jaspis,
Geodia,
Stelletta
Rhabdastrella.
From
total
44
articles
(1981-2022),
27
unique
vouchers
were
listed,
21
which
accessed
re-examined
here:
11
(52.4%)
these
misidentified.
Overall,
65.9%
studies
published
incorrect
species
name:
previously
identified
Jaspis
all
fact
Rhabdastrella
globostellata.
We
conclude
that
triterpenes
only
two
possibly
one
Geodia
species.
In
addition
shedding
new
light
on
distribution
this
study
opportunity
highlight
crucial
importance
product
studies.
Doing
so,
discuss
impact
poor
accessibility
products.
advocate
for
stricter
voucher
guidelines
journals
propose
common
protocol
good
practice,
hope
reducing
misidentifications
ensure
reproducibility
facilitate
follow-up
work
original
material.
Microbiology Research,
Год журнала:
2023,
Номер
15(1), С. 66 - 90
Опубликована: Дек. 21, 2023
This
article
explores
the
diverse
array
of
biologically
active
compounds
derived
from
microbial
symbionts,
particularly
focusing
on
isolation
and
characterization
diepoxides,
highly
oxygenated
triterpenoids,
secosteroids,
ergostane-type
steroids,
meroterpenoids
various
marine
plant-derived
fungi.
We
highlight
significant
discoveries
such
as
vitamin
D
variants
fungal
species,
unique
sesterterpenoids
mangrove
endophytic
fungi,
secosteroids
with
potential
medicinal
applications.
The
study
delves
into
structural
uniqueness
bioactivities
these
compounds,
including
their
anti-inflammatory,
antibacterial,
antifungal,
antiviral,
cytotoxic
effects.
Notable
findings
include
activity
against
cancer
cell
lines,
inhibition
acetylcholinesterase,
promising
antifouling
properties.
work
underscores
symbionts
a
rich
source
novel
bioactive
therapeutic
applications,
highlighting
importance
biodiversity
in
drug
discovery
development.
Journal of Applied Microbiology,
Год журнала:
2022,
Номер
133(3), С. 2027 - 2038
Опубликована: Июль 12, 2022
This
work
aims
to
characterize
the
microbial
diversity
of
encrusting
sponge
Cliona
varians,
a
pore-forming
and
coral
reef
bioeroding
marine
emerging
spread
related
ocean
acidification.We
analysed
microbiome
composition
by
16S
V4
amplicon
next-generation
sequencing
in
community
encrusting/excavating
varians
thriving
at
Southern
Caribbean
Sea.
About
87.21%
6.76%
sequences
retrieved
were
assigned
domain
Bacteria
Archaea.
The
most
predominant
operational
taxonomic
units
classified
as
members
order
Rhizobiales
family
Nitrosopumilaceae,
representing
not
yet
characterized
genera.
Features
found
strictly
conserved
strain/genomic
representatives
reported
those
taxa
are
nitrogen
fixation
transformation.Our
results
suggest,
accordance
with
recent
results,
that
these
associated
functions
could
be
contributing
biological
fitness
able
colonize
bioerode
environments
low
access
scarce
availability
sources.Coral
reefs
bioresources
such
holobionts
intriguing
complex
ecosystem
units.
study
contributes
knowledge
how
C.
microbiota
is
composed
or
shaped,
which
crucial
understand
its
ecological
functions.