The phylum Chloroflexi and their SAR202 clade dominate the microbiome of two marine sponges living in extreme environmental conditions

Marine Ecology, Год журнала: 2023, Номер 44(5)

Опубликована: Май 29, 2023

Abstract The capacity of marine sponges to cope with environmental changes is often attributed the specific composition their bacterial communities. In this study, we assessed microbiome two dominant sponges, Rhabdastrella globostellata (Rg) and Hyrtios erectus (He), living in Bouraké lagoon (New Caledonia), where abiotic conditions daily fluctuate according tide. Sponge specimens, sediment seawater samples were collected at 2–3 m depth. communities using 16S rRNA metabarcoding, variations between compared PCA biplots. Chloroflexi was phyla both He Rg an average relative abundance 41.2% 53.2%, respectively, while it absent seawater. Among phylum Chloroflexi, SAR202 clade reaching 53.2% (He) 78.7% (Rg). Principal component analysis (PCA) used identify main variables driving community structure sponges. results indicated that strongly associated (70.9% variance) (86.6% variance). high observed study highest reported so far literature Such a these bacteria could suggest involvement well‐being extreme Bouraké.

Язык: Английский

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Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths

Marine Drugs, Год журнала: 2019, Номер 17(10), С. 578 - 578

Опубликована: Окт. 11, 2019

Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens below 30 m. This study assessed the cultivability of two marine sponges Xestospongia muta and Agelas sventres collected (<30 m), upper mesophotic (30–60 lower (60–90 reefs. were cultivated on six different media, replicate plates used to pick individual colonies or recover entire biomass. Prokaryotic community analysis was conducted using Illumina MiSeq sequencing 16S rRNA gene amplicons. A total 144 bacterial isolates picked following a colony morphology coding scheme subsequently identified by sequence analysis. Sponge individuals at each depth-range harboured specific cultivable that not retrieved other depths. However, there substantial differences in number obtained for same species. In addition, source inoculum cultivation medium had more impact prokaryotic than sample collection depth. suggests “plate count anomaly” is larger sponge-associated composition related

Язык: Английский

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Not That Close to Mommy: Horizontal Transmission Seeds the Microbiome Associated with the Marine Sponge Plakina cyanorosea

Microorganisms, Год журнала: 2020, Номер 8(12), С. 1978 - 1978

Опубликована: Дек. 12, 2020

Marine sponges are excellent examples of invertebrate–microbe symbioses. In this holobiont, the partnership has elegantly evolved by either transmitting key microbial associates through host germline and/or capturing microorganisms from surrounding seawater. We report here on prokaryotic microbiota during different developmental stages Plakina cyanorosea and their environmental samples a 16S rRNA metabarcoding approach. comparison with source adults, larvae housed slightly richer more diverse communities, which structurally related to microbiota. addition thaumarchaeal Nitrosopumilus, parental were broadly dominated Alpha- Gamma-proteobacteria, while offspring particularly enriched in Vibrionales, Alteromonodales, Enterobacterales orders Clostridia Bacteroidia classes. An enterobacterial operational taxonomic unit (OTU) was dominant member strict core The most abundant unique OTUs not significantly amongst microbiomes specimens included sponge microbiome project. wider context, Oscarella genera higher divergence associated compared Homoscleromorpha counterparts. Our results indicate that P. is low abundance (LMA), appears heavily depend horizontal transmission its partners likely help adaptation habitat.

Язык: Английский

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Sponge holobionts shift their prokaryotic communities and antimicrobial activity from shallow to lower mesophotic depths

Antonie van Leeuwenhoek, Год журнала: 2022, Номер 115(10), С. 1265 - 1283

Опубликована: Авг. 23, 2022

In this study, we used 16S rRNA gene amplicon sequencing to investigate prokaryotic community composition of the Caribbean sponges Xestospongia muta and Agelas sventres from three depth ranges: < 30 m (shallow), 30-60 (upper mesophotic), 60-90 (lower mesophotic). The in shallow samples X. was enriched Cyanobacteria, Chloroflexota, Crenarchaeota compared mesophotic depths, while were Acidobacteriota. For A. sventres, relative abundance Acidobacteriota, Gammaproteobacteria higher samples, Proteobacteria samples. Antimicrobial activity evaluated by screening crude extracts against a set Gram-positive Gram-negative bacteria, yeast, an oomycete. Antibacterial activities sponge individuals generally than observed individuals, that showed limited or no antibacterial activities. Conversely, highest anti-oomycete found lower depth, but without clear pattern across gradient. These results indicate sponge-associated communities antimicrobial change within species gradient depth.

Язык: Английский

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Diversity of Bacterial Secondary Metabolite Biosynthetic Gene Clusters in Three Vietnamese Sponges

Marine Drugs, Год журнала: 2022, Номер 21(1), С. 29 - 29

Опубликована: Дек. 29, 2022

Recent reviews have reinforced sponge-associated bacteria as a valuable source of structurally diverse secondary metabolites with potent biological properties, which makes these microbial communities promising sources new drug candidates. However, the overall diversity metabolite biosynthetic potential present in is difficult to access due fact that majority are not readily cultured laboratory. Thus, use cultivation-independent approaches may allow accessing “silent” and “cryptic” gene clusters cannot yet be cultured. In study, we investigated (BGCs) metagenomes bacterial associated three sponge species: Clathria reinwardti, Rhabdastrella globostellata, Spheciospongia sp. The results reveal contain high number predicted BGCs, ranging from 282 463 BGCs per metagenome. types were represented 12 different cluster types. Clusters encode fatty acid synthases polyketide (PKS) most dominant BGC types, followed by encoding synthesis terpenes bacteriocins. Based on sequence similarity analysis, 363 families (GCFs) identified. Interestingly, no GCFs assigned pathways responsible for production known compounds, implying detected might several novel compounds. KS sequences PKS used predict taxonomic origin involved. related phyla Actinobacteria, Proteobacteria, Firmicutes predominant. At genus level, KSs those found genera Mycolicibacterium, Mycobacterium, Burkholderia, Streptomyces. Phylogenetic analysis resulted detection two ‘sponge-specific’ i.e., SupA SwfA, well phylum Candidatus Poribacteria composed only assessed here.

Язык: Английский

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Good Practices in Sponge Natural Product Studies: Revising Vouchers with Isomalabaricane Triterpenes

Marine Drugs, Год журнала: 2022, Номер 20(3), С. 190 - 190

Опубликована: Март 4, 2022

Species misidentification in the field of natural products is an acknowledged problem. These errors are especially widespread sponge studies, albeit rarely assessed and documented. As a case study, we aim to revisit reports isomalabaricane triterpenes, isolated from four demosponge genera: Jaspis, Geodia, Stelletta Rhabdastrella. From total 44 articles (1981-2022), 27 unique vouchers were listed, 21 which accessed re-examined here: 11 (52.4%) these misidentified. Overall, 65.9% studies published incorrect species name: previously identified Jaspis all fact Rhabdastrella globostellata. We conclude that triterpenes only two possibly one Geodia species. In addition shedding new light on distribution this study opportunity highlight crucial importance product studies. Doing so, discuss impact poor accessibility products. advocate for stricter voucher guidelines journals propose common protocol good practice, hope reducing misidentifications ensure reproducibility facilitate follow-up work original material.

Язык: Английский

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Temporal dynamics of the bacterial community structure and functions associated with marine sponges collected off Karah Island, Terengganu, Malaysia

The Microbe, Год журнала: 2024, Номер 5, С. 100202 - 100202

Опубликована: Ноя. 10, 2024

Язык: Английский

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Actinobacteria from Marine Environments: A Unique Source of Natural Products

Springer eBooks, Год журнала: 2022, Номер unknown, С. 1 - 45

Опубликована: Янв. 1, 2022

Язык: Английский

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Bioactive Diepoxy Metabolites and Highly Oxygenated Triterpenoids from Marine and Plant-Derived Bacteria and Fungi

Microbiology Research, Год журнала: 2023, Номер 15(1), С. 66 - 90

Опубликована: Дек. 21, 2023

This article explores the diverse array of biologically active compounds derived from microbial symbionts, particularly focusing on isolation and characterization diepoxides, highly oxygenated triterpenoids, secosteroids, ergostane-type steroids, meroterpenoids various marine plant-derived fungi. We highlight significant discoveries such as vitamin D variants fungal species, unique sesterterpenoids mangrove endophytic fungi, secosteroids with potential medicinal applications. The study delves into structural uniqueness bioactivities these compounds, including their anti-inflammatory, antibacterial, antifungal, antiviral, cytotoxic effects. Notable findings include activity against cancer cell lines, inhibition acetylcholinesterase, promising antifouling properties. work underscores symbionts a rich source novel bioactive therapeutic applications, highlighting importance biodiversity in drug discovery development.

Язык: Английский

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Microbiome composition of the marine sponge Cliona varians at the neotropical southern Caribbean Sea displays a predominant core of Rhizobiales and Nitrosopumilaceae

Journal of Applied Microbiology, Год журнала: 2022, Номер 133(3), С. 2027 - 2038

Опубликована: Июль 12, 2022

This work aims to characterize the microbial diversity of encrusting sponge Cliona varians, a pore-forming and coral reef bioeroding marine emerging spread related ocean acidification.We analysed microbiome composition by 16S V4 amplicon next-generation sequencing in community encrusting/excavating varians thriving at Southern Caribbean Sea. About 87.21% 6.76% sequences retrieved were assigned domain Bacteria Archaea. The most predominant operational taxonomic units classified as members order Rhizobiales family Nitrosopumilaceae, representing not yet characterized genera. Features found strictly conserved strain/genomic representatives reported those taxa are nitrogen fixation transformation.Our results suggest, accordance with recent results, that these associated functions could be contributing biological fitness able colonize bioerode environments low access scarce availability sources.Coral reefs bioresources such holobionts intriguing complex ecosystem units. study contributes knowledge how C. microbiota is composed or shaped, which crucial understand its ecological functions.

Язык: Английский

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