Combined Study of Gene Expression and Chromosome Three-Dimensional Structure in Escherichia coli During Growth Process

Current Microbiology, Год журнала: 2024, Номер 81(5)

Опубликована: Март 26, 2024

Язык: Английский

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Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

AMB Express, Год журнала: 2017, Номер 7(1)

Опубликована: Май 12, 2017

Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction environmental stimuli are still not clear. The rearrangement metabolism response stress could be controlled by stringent that modulates transcription genes order promote survival under nutritional deprivation conditions. Therefore, this work we investigated relation between and response. For study, relA/spoT mutant KT2440, unable induce response, was used. Additionally, transcriptome analyzed RNA-seq examine rearrangements during cultivation. results show able accumulate both optimal nitrogen limiting Nitrogen starvation did change efficiency mutant. transition from exponential growth stationary phase caused significant upregulation involved transport system metabolism. Transcriptional regulators, including rpoS, rpoN rpoD, changes transcript abundance when entering phase, suggesting their limited role accumulation phase.

Язык: Английский

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Resistance of early stationary phase E. coli to membrane permeabilization by the antimicrobial peptide Cecropin A

Biochimica et Biophysica Acta (BBA) - Biomembranes, Год журнала: 2019, Номер 1861(10), С. 182990 - 182990

Опубликована: Май 23, 2019

Язык: Английский

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The bile salt glycocholate induces global changes in gene and protein expression and activates virulence in enterotoxigenic Escherichia coli

Scientific Reports, Год журнала: 2019, Номер 9(1)

Опубликована: Янв. 8, 2019

Abstract Pathogenic bacteria use specific host factors to modulate virulence and stress responses during infection. We found previously that the factor bile component glyco-conjugated cholate (NaGCH, sodium glycocholate) upregulate colonization CS5 in enterotoxigenic Escherichia coli (ETEC). To further understand global regulatory effects of NaGCH, we performed Illumina RNA-Seq crude NaGCH altered expression 61 genes + CS6 ETEC isolates. The most striking finding was high induction operon ( csfA-F ), its putative transcription csvR , cexE . iTRAQ-coupled LC-MS/MS proteomic analyses verified plasmid-borne proteins CexE also showed affected bacterial membrane proteins. Furthermore, induced aggregate, increased their adherence epithelial cells, reduced motility. Our results indicate present small intestine as a signal initiate epithelium.

Язык: Английский

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The Stringent Stress Response Controls Proteases and Global Regulators under Optimal Growth Conditions in Pseudomonas aeruginosa

mSystems, Год журнала: 2020, Номер 5(4)

Опубликована: Авг. 3, 2020

Microorganisms need to adapt rapidly survive harsh environmental changes. Here, we showed the broad influence of highly studied bacterial stringent stress response under nonstressful conditions that indicate its general physiological importance and might reflect readiness bacteria respond activate acute responses. Using RNA-Seq investigate transcriptional network Pseudomonas aeruginosa cells revealed >30% all genes changed expression in a mutant optimal growth conditions. This included regulated by global regulators novel downstream effectors. Our results help understand this regulator lifestyle relatively unstressed As such, it draws attention consequences targeting ubiquitous signaling molecule.

Язык: Английский

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