FEBS Journal, Год журнала: 2023, Номер 290(23), С. 5566 - 5580
Опубликована: Авг. 27, 2023
N-carbamoyl-β-alanine amidohydrolase (CβAA) constitutes one of the most important groups industrially relevant enzymes used in production optically pure amino acids and derivatives. In this study, a CβAA-encoding gene from Rhizobium radiobacter strain MDC 8606 was cloned overexpressed Escherichia coli. The purified recombinant enzyme (RrCβAA) showed specific activity 14 U·mg
Язык: Английский
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Окт. 4, 2023
Cytosolic metalloenzymes acquire metals from buffered intracellular pools. How exported are appropriately metalated is less clear. We provide evidence that TerC family proteins function in metalation of enzymes during export through the general secretion (Sec-dependent) pathway. Bacillus subtilis strains lacking MeeF(YceF) and MeeY(YkoY) have a reduced capacity for protein greatly level manganese (Mn) secreted proteome. MeeF MeeY copurify with secretory pathway, their absence FtsH membrane protease essential viability. also required efficient Mn
Язык: Английский
Процитировано
16
Annual Review of Plant Biology, Год журнала: 2024, Номер 75(1), С. 27 - 65
Опубликована: Янв. 26, 2024
One of life's decisive innovations was to harness the catalytic power metals for cellular chemistry. With expansion, global atmospheric and biogeochemical cycles underwent dramatic changes. Although initially harmful, they permitted evolution multicellularity colonization land. In land plants as primary producers, metal homeostasis faces heightened demands, in part because soil is a challenging environment nutrient balancing. To avoid both limitation toxicity, must maintain within tighter limits than other minerals. This review describes present model protein metalation sketches its transfer from unicellular organisms complex multicellular organisms. The inseparable connection between redox increasingly draws our attention more general regulatory roles metals. Mineral co-option, use or functions nutrition, an emerging concept beyond that nutritional immunity.
Язык: Английский
Процитировано
5
Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15
Опубликована: Фев. 12, 2025
Introduction Zinc exhibits potent antimicrobial properties due to its ability compromise bacterial structure and protein functionality, effectively inhibiting eradicating bacteria. However, bacteria have evolved mechanisms expel excess zinc ions from their cells, enabling them thrive in environments rich metal at high concentrations. This evolutionary advancement limits the clinical application of as agents. In this study, we aimed investigate potential ionophores overcome resistance by elevating intracellular ion levels. Methods We employed ionophore PBT2 elevate levels Klebsiella pneumoniae, a bacterium known for various antibiotics. By treating K. pneumoniae with PBT2, assess impact on tigecycline, an antibiotic commonly used settings. The changes levels, superoxide dismutase activity, reactive oxygen species concentration, cell wall synthesis pathway were monitored evaluate mechanism action PBT2. Results Our results revealed that successfully reversed tigecycline. Specifically, increased concentration leading suppression activity within elevation concentration. These impaired oxidative stress response Additionally, disruption homeostasis significantly inhibited potentially restricting efflux pump predominantly drives tigecycline resistance. Discussion findings study pave way innovative strategies approaches development novel using such can antibiotics like suggest impairs further enhancing susceptibility Furthermore, inhibition restriction provide additional which reverses highlight class agents warrant investigation into applications.
Язык: Английский
Процитировано
0
Quantitative Plant Biology, Год журнала: 2025, Номер 6
Опубликована: Янв. 1, 2025
Abstract The micronutrient zinc (Zn) is often poorly available but toxic when present in excess, so a tightly controlled Zn homoeostasis network operates all organisms. This review summarizes our understanding of plant homoeostasis. In Arabidopsis , about 1,900 Zn-binding metalloproteins require as cofactor. Abundant reside plastids, mitochondria and peroxisomes, emphasizing the need to address how reaches these proteins. Apo–Zn do not acquire 2+ from cytosolic pool free cations, instead through associative ligand exchange Zn-buffering molecules. importance thiols buffering suggests that, besides elevated influx, more oxidized redox state also predicted cause labile-bound levels, consistent with suppression deficiency marker under oxidative stress. Therefore, we consider broadened physiological scope plants for possible signalling role experimentally supported only animals date.
Язык: Английский
Процитировано
0
Annual Review of Microbiology, Год журнала: 2024, Номер 78(1), С. 83 - 102
Опубликована: Июль 4, 2024
Cell physiology requires innumerable metalloenzymes supported by the selective import of metal ions. Within crowded cytosol, most enzymes acquire their cognate cofactors from a buffered labile pool. Metalation membrane-bound and secreted exoenzymes is more problematic since concentrations are highly variable outside cell. Here, we focus on involved in cell envelope homeostasis. Peptidoglycan synthesis often relies Zn-dependent hydrolases, metal-dependent β-lactamases play important roles antibiotic resistance. In gram-positive bacteria, lipoteichoic acid Mn, with TerC family Mn exporters supporting role. For some exoenzymes, metalation occurs metalated exported through TAT secretion system. others, facilitated exporters, metallochaperones, or partner proteins that enhance affinity. To help ensure function, can function multiple metals. Thus, cells employ diversity strategies to functioning cytosol.
Язык: Английский
Процитировано
3
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(38)
Опубликована: Сен. 9, 2024
Modern life requires many different metal ions, which enable diverse biochemical functions. It is commonly assumed that ions’ environmental availabilities controlled the evolution of early life. We argue can only explore chemistry encounters, and fortuitous chemical interactions between ions biological compounds be selected for if they first occur sufficiently frequently. calculated maximal transition ion concentrations in ancient ocean, determining amounts biologically important were orders magnitude lower than ferrous iron. Under such conditions, primitive bioligands would predominantly interact with Fe(II). While other metals certain environments may have provided evolutionary opportunities, capacities Fe(II), Fe–S clusters, or plentiful magnesium calcium could satisfied all functions needed by Primitive organisms used Fe(II) exclusively their requirements.
Язык: Английский
Процитировано
3
Journal of Bacteriology, Год журнала: 2024, Номер 206(6)
Опубликована: Май 31, 2024
Microbes encounter a myriad of stresses during their life cycle. Dysregulation metal ion homeostasis is increasingly recognized as key factor in host-microbe interactions. Bacterial tightly regulated by dedicated metalloregulators that control uptake, sequestration, trafficking, and efflux. Here, we demonstrate deletion the
Язык: Английский
Процитировано
2
Biochemistry, Год журнала: 2024, Номер 63(14), С. 1709 - 1717
Опубликована: Июль 8, 2024
I present the perspective that divalent metalome and metabolome can be modeled as a network of chelating interactions instead separate entities. review progress in understanding complex cellular environment, particular recent contributions to modeling metabolite-Mg
Язык: Английский
Процитировано
2
Molecular Microbiology, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 11, 2024
Pathogenic fungi must appropriately sense the host availability of essential metals such as Fe. In Candida albicans and other yeasts, sensing Fe involves mitochondrial Fe-S clusters. Yeast mutants for cluster assembly limitation even when is abundant hyperaccumulate We observe this same disrupted with C. SMF11, a NRAMP transporter divalent metals. Mutants smf11 both Mn elevated secondary to overload. As biogenesis mutants, smf11∆/∆ show upregulation ferric reductases that are normally repressed under high Fe, import activated. However, unlike no defects in enzymes. Intriguingly, exact condition without inhibiting clusters occurs fre1∆/∆ encoding reductase. fre1 display similar perturbations cell wall, filamentation ROS burst morphogenesis, Fe-dependent process. FRE1, SMF11 important virulence mouse model disseminated candidiasis. propose which FRE1 operate outside pathway donate ferrous sensing.
Язык: Английский
Процитировано
2
Journal of Bacteriology, Год журнала: 2024, Номер 206(8)
Опубликована: Авг. 22, 2024
The metal-resistant beta-proteobacterium
Язык: Английский
Процитировано
2