From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Advanced Functional Materials, Год журнала: 2020, Номер 30(37)

Опубликована: Июнь 11, 2020

From micro-scaled capillaries to millimeter-sized arteries and veins, human vasculature spans multiple scales cell types. The convergence of bioengineering, materials science, stem biology has enabled tissue engineers recreate the structure function different hierarchical levels vascular tree. Engineering large-scale vessels been pursued over past thirty years replace or bypass damaged arteries, arterioles, venules, their routine application in clinic may become a reality near future. Strategies engineer meso- microvasculature have extensively explored generate models study biology, drug transport, disease progression, as well for vascularizing engineered tissues regenerative medicine. However, bioengineering whole organs transplantation, failed result clinical translation due lack proper integrated effective oxygen nutrient delivery. development strategies multi-scale networks direct anastomosis host would greatly benefit this formidable goal. In review, we discuss design considerations technologies engineering millimeter-, meso-, micro-scale vessels. We further provide examples recent state-of-the-art vasculature. Finally, identify key challenges limiting vascularized offer our perspective on future directions exploration.

Язык: Английский

---

Organs-on-chips technologies – A guide from disease models to opportunities for drug development

Biosensors and Bioelectronics, Год журнала: 2023, Номер 231, С. 115271 - 115271

Опубликована: Март 31, 2023

Current in-vitro 2D cultures and animal models present severe limitations in recapitulating human physiopathology with striking discrepancies estimating drug efficacy side effects when compared to trials. For these reasons, microphysiological systems, organ-on-chip multiorgans microdevices attracted considerable attention as novel tools for high-throughput high-content research achieve an improved understanding of diseases accelerate the development process towards more precise eventually personalized standards. This review takes form a guide on this fast-growing field, providing useful introduction major themes indications further readings. We start analyzing Organs-on-chips (OOC) technologies testing administration routes: (1) oral/rectal route by intestine-on-a-chip, (2) inhalation lung-on-a-chip, (3) transdermal skin-on-a-chip (4) intravenous through vascularization models, considering how drugs penetrate bloodstream are conveyed their targets. Then, we focus OOC (other) specific organs diseases: neurodegenerative brain blood barriers, tumor including vascularization, organoids/spheroids, engineering screening antitumor drugs, liver/kidney chips multiorgan gastrointestinal metabolic assessment biomechanical systems heart, muscles bones structures related diseases. Successively, discuss materials organ chips, microfluidic organs-on-chips, sensor integration real-time monitoring, cell lines chips. (Nano)delivery approaches therapeutics chip also described. Finally, conclude critical discussion current significance/relevance, trends, limitations, challenges future prospects terms revolutionary impact biomedical research, preclinical development.

Язык: Английский

---

Advancements in tissue and organ 3D bioprinting: Current techniques, applications, and future perspectives

Materials & Design, Год журнала: 2024, Номер 240, С. 112853 - 112853

Опубликована: Март 19, 2024

3D bioprinting techniques have emerged as a flexible tool in tissue engineering and regenerative medicine to fabricate or pattern functional bio-structures with precise geometric designs, bridging the divergence between engineered natural constructs. A significantly increasing development has been achieved understanding relationship 3D-printing process structures, properties, applications of objects created. The ongoing advancement novel biomaterial inks enabled manufacturing models vitro implants capable achieving some level success preclinical trials. Remarkable progress cell biology biology-inspired computational design assisted latest milestone planned tissue- organ-like constructs having specific levels functionality. However, biofabricated still long way go before reaching clinics. This review presents picture context medicine, focus on biomaterials-related design-centred aspects. Biomedical are described detail relation major tissues organs considered human body. Current technical limitations, challenges, future prospects improvements critically outlined discussed.

Язык: Английский

---

Engineering Tissue Barrier Models on Hydrogel Microfluidic Platforms

ACS Applied Materials & Interfaces, Год журнала: 2021, Номер 13(12), С. 13920 - 13933

Опубликована: Март 19, 2021

Tissue barriers play a crucial role in human physiology by establishing tissue compartmentalization and regulating organ homeostasis. At the interface between extracellular matrix (ECM) flowing fluids, epithelial endothelial are responsible for solute gas exchange. In past decade, microfluidic technologies organ-on-chip devices became popular as vitro models able to recapitulate these biological barriers. However, conventional devices, cell primarily grown on hard polymeric membranes within polydimethylsiloxane (PDMS) channels that do not mimic cell–ECM interactions nor allow incorporation of other cellular compartments such stromal or vascular structures. To develop accurately account different acellular barriers, researchers have integrated hydrogels into setups barrier-on-chips, either substrates inside chip, self-contained devices. These biomaterials provide soft mechanical properties embedding cells. Combining with microfluidics technology provides unique opportunities better recreate barrier including components functionality vivo tissues. Such platforms potential greatly improving predictive capacities systems applications drug development, disease modeling. Nevertheless, their development is without challenges microfabrication. this review, we will discuss recent advances driving fabrication hydrogel multiple models.

Язык: Английский

---

Cell-matrix reciprocity in 3D culture models with nonlinear elasticity

Bioactive Materials, Год журнала: 2021, Номер 9, С. 316 - 331

Опубликована: Авг. 14, 2021

Three-dimensional (3D) matrix models using hydrogels are powerful tools to understand and predict cell behavior. The interactions between the its matrix, however is highly complex: has a profound effect on basic functions but simultaneously, cells able actively manipulate properties. This (mechano)reciprocity extracellular (ECM) central in regulating tissue it fundamentally important broadly consider biomechanical properties of vivo ECM when designing vitro models. manuscript discusses two commonly used biopolymer networks, i.e. collagen fibrin gels, one synthetic polymer network, polyisocyanide gel (PIC), which all possess characteristic nonlinear mechanics biological stress regime. We start from structure materials, then address uses, advantages, limitations each material, provide guideline for engineers biophysicists utilizing current materials also new 3D culture purposes.

Язык: Английский

---

Strategies for Delivering Nanoparticles across Tumor Blood Vessels

Advanced Functional Materials, Год журнала: 2020, Номер 31(8)

Опубликована: Ноя. 12, 2020

Abstract Nanoparticle transport across tumor blood vessels is a key step in nanoparticle delivery to solid tumors. However, the specific pathways and mechanisms of this process are not fully understood. Here, biological physical characteristics vasculature microenvironment explored how these features affect discussed. The methods deliver nanoparticles into tumors reviewed paracellular transcellular explored. Understanding underlying will inform engineering safer more effective nanomedicines for clinical translation.

Язык: Английский

---

3D Bioprinted Multicellular Vascular Models

Advanced Healthcare Materials, Год журнала: 2021, Номер 10(21)

Опубликована: Июль 26, 2021

3D bioprinting is an emerging additive manufacturing technique to fabricate constructs for human disease modeling. However, current cell-laden bioinks lack sufficient biocompatibility, printability, and structural stability needed translate this technology preclinical clinical trials. Here, a new class of nanoengineered hydrogel-based introduced, that can be printed into 3D, anatomically accurate, multicellular blood vessels recapitulate both the physical chemical microenvironments native vasculature. A remarkably unique characteristic bioink regardless cell density, it demonstrates high printability ability protect encapsulated cells against shear forces in process. bioprinted maintain healthy phenotype remain viable nearly one-month post-fabrication. Leveraging these properties, cylindrical vessels, consisting living co-culture endothelial vascular smooth muscle cells, providing opportunity model function pathophysiology. Upon cytokine stimulation perfusion, vessel able thromboinflammatory responses observed only advanced vitro models or vivo. Therefore, provides potential tool understand pathophysiology assess therapeutics, toxins, other chemicals.

Язык: Английский

---

Fibrinogen-mimicking, multiarm nanovesicles for human thrombus-specific delivery of tissue plasminogen activator and targeted thrombolytic therapy

Science Advances, Год журнала: 2021, Номер 7(23)

Опубликована: Июнь 2, 2021

Bioinspired nanovesicles enable activated platelet–targeted delivery of tissue plasminogen activator for effective thrombolysis.

Язык: Английский

---

A Review of Single-Cell Adhesion Force Kinetics and Applications

Cells, Год журнала: 2021, Номер 10(3), С. 577 - 577

Опубликована: Март 5, 2021

Cells exert, sense, and respond to the different physical forces through diverse mechanisms translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as maintain tissue morphogenesis homeostasis activate critical signaling pathways regulating survival, migration, gene expression, differentiation. More importantly, any mutations receptors can lead disorders diseases. Thus, it essential understand regulation cell during development its contribution conditions with help quantitative methods. techniques involved offering functionalities surface imaging detect present at cell-matrix deliver parameters will characterize changes for Here, we have briefly reviewed single-cell mechanical properties mechanotransduction studies using standard recently developed techniques. This used functionalize from measurement cellular deformability quantification interaction generated by a exerted on surroundings attachment detachment events. adhesive force microorganisms single-molecules emphasized well. focused review should be useful laying out experiments which would bring method broader range research future.

Язык: Английский

---

Nanoparticles for Thrombus Diagnosis and Therapy: Emerging Trends in Thrombus-theranostics

Nanotheranostics, Год журнала: 2024, Номер 8(2), С. 127 - 149

Опубликована: Янв. 1, 2024

Cardiovascular disease is one of the chief factors that cause ischemic stroke, myocardial infarction, and venous thromboembolism.The elements speed up thrombosis include nutritional consumption, physical activity, oxidative stress.Even though precise etiology pathophysiology remain difficult topics primarily rely on traditional medicine.The diagnosis management are being developed using discrete non-invasive non-surgical approaches.One emerging promising approach ultrasound photoacoustic imaging.The advancement nanomedicines offers concentrated therapy diagnosis, imparting efficacy fewer side effects which more significant than conventional medicine.This study addresses potential as theranostic agents for treatment thrombosis.In this article, we describe lead to its consequences, well summarize findings studies thrombus formation in preclinical clinical models also provide insights nanoparticles imaging therapy.

Язык: Английский

---