Molecular imaging nanoprobes for theranostic applications

Advanced Drug Delivery Reviews, Год журнала: 2022, Номер 186, С. 114320 - 114320

Опубликована: Май 6, 2022

Язык: Английский

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Immunotherapy discovery on tumor organoid-on-a-chip platforms that recapitulate the tumor microenvironment

Advanced Drug Delivery Reviews, Год журнала: 2022, Номер 187, С. 114365 - 114365

Опубликована: Июнь 4, 2022

Язык: Английский

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Recent progress of microfluidic chips in immunoassay

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Дек. 23, 2022

Microfluidic chip technology is a platform that integrates basic operation units such as processing, separation, reaction and detection into microchannel to realize low consumption, fast efficient analysis of samples. It has the characteristics small volume need samples reagents, analysis, cost, automation, portability, high throughout, good compatibility with other techniques. In this review, concept, preparation materials fabrication microfluidic are described. The applications in immunoassay, including fluorescent, chemiluminescent, surface-enhanced Raman spectroscopy (SERS), electrochemical immunoassay reviewed. Look future, development chips lies point-of-care testing throughput equipment, there still some challenges design integration chips, well actual sample by chips.

Язык: Английский

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Blood–Brain Barrier Breakdown in Neuroinflammation: Current In Vitro Models

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(16), С. 12699 - 12699

Опубликована: Авг. 11, 2023

The blood-brain barrier, which is formed by tightly interconnected microvascular endothelial cells, separates the brain from peripheral circulation. Together with other central nervous system-resident cell types, including pericytes and astrocytes, barrier forms neurovascular unit. Upon neuroinflammation, this becomes leaky, allowing molecules cells to enter potentially harm tissue of system. Despite significance animal models in research, they may not always adequately reflect human pathophysiology. Therefore, are needed. This review will provide an overview terms both health disease. It describe all key elements vitro explore how different compositions can be utilized effectively model a variety neuroinflammatory conditions. Furthermore, it existing types that used basic research study respective pathologies thus far.

Язык: Английский

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A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements

Biofabrication, Год журнала: 2024, Номер 16(3), С. 035008 - 035008

Опубликована: Апрель 4, 2024

Abstract Conventional gut-on-chip (GOC) models typically represent the epithelial layer of gut tissue, neglecting other important components such as stromal compartment and extracellular matrix (ECM) that play crucial roles in maintaining intestinal barrier integrity function. These often employ hard, flat porous membranes for cell culture, thus failing to recapitulate soft environment complex 3D architecture mucosa. Alternatively, hydrogels have been recently introduced GOCs ECM analogs support co-culture cells vivo -like configurations, opening new opportunities organ-on-chip field. In this work, we present an innovative GOC device includes a bioprinted hydrogel channel replicating villi containing both compartments The successfully encapsulation fibroblasts their with under physiological flow conditions. Moreover, integrated electrodes into microfluidic system monitor formation real time via transepithelial electrical resistance measurements.

Язык: Английский

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On-chip recapitulation of the tumor microenvironment: A decade of progress

Biomaterials, Год журнала: 2024, Номер 306, С. 122482 - 122482

Опубликована: Янв. 22, 2024

One of the hurdles to development new anticancer therapies is lack in vitro models which faithfully reproduce vivo tumor microenvironment (TME). Understanding dynamic relationships between components TME a controllable, scalable, and reliable setting would indeed support discovery biological targets impacting cancer diagnosis therapy. Cancer research increasingly shifting from traditional two-dimensional (2D) cell culture toward three-dimensional (3D) models, have been demonstrated increase significance predictive value data. In this scenario, microphysiological systems (also known as Organs-on-Chip) emerged relevant technological platform enabling more investigation cell-cell cell-ECM interplay cancer, attracting significant effort last years. This review illustrates one decade progress field tumor-microenvironment-on-chip (TMOC) approaches, exploiting either cell-laden microfluidic chambers or confined spheroids model TME. TMOCs designed recapitulate several aspects TME, including cells, tumor-associated stroma, immune system, vascular component. Significantly, aspect has for its pivotal role orchestrating cellular interactions modulating drug pharmacokinetics on-chip. A further advancement represented by integration into multi-organ systems, with final aim follow metastatic cascade target organs study effects chemotherapies at systemic level. We highlight that increased degree complexity achieved most advanced TMOC enabled scientists shed light on microenvironmental factors progression, cascade, response drugs.

Язык: Английский

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Lung-on-a-chip: From design principles to disease applications

Biomicrofluidics, Год журнала: 2025, Номер 19(2)

Опубликована: Март 1, 2025

To address the growing need for accurate lung models, particularly in light of respiratory diseases, cancer, and COVID-19 pandemic, lung-on-a-chip technology is emerging as a powerful alternative. Lung-on-a-chip devices utilize microfluidics to create three-dimensional models that closely mimic key physiological features human lung, such air-liquid interface, mechanical forces associated with respiration, fluid dynamics. This review provides comprehensive overview fundamental components systems, diverse fabrication methods used construct these complex summary their wide range applications disease modeling aerosol deposition studies. Despite existing challenges, hold immense potential advancing personalized medicine, drug development, prevention, offering transformative approach health research.

Язык: Английский

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Kidney-on-a-Chip: Mechanical Stimulation and Sensor Integration

Sensors, Год журнала: 2022, Номер 22(18), С. 6889 - 6889

Опубликована: Сен. 13, 2022

Bioengineered in vitro models of the kidney offer unprecedented opportunities to better mimic vivo microenvironment. Kidney-on-a-chip technology reproduces 2D or 3D features which can replicate tissue architecture, composition, and dynamic mechanical forces experienced by cells vivo. Kidney are exposed stimuli such as substrate stiffness, shear stress, compression, stretch, regulate multiple cellular functions. Incorporating kidney-on-a-chip is critically important for recapitulating physiological pathological This review will explore approaches applying different cell types using how these systems used study physiology, model disease, screen drug toxicity. We further discuss sensor integration into monitoring responses other stimuli. advantages, limitations, challenges associated with incorporating a variety applications. Overall, this aims highlight importance design implementation devices.

Язык: Английский

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Basement membrane properties and their recapitulation in organ-on-chip applications

Materials Today Bio, Год журнала: 2022, Номер 15, С. 100301 - 100301

Опубликована: Май 23, 2022

Drug discovery and toxicology is a complex process that involves considerable basic research preclinical evaluation. These depend highly on animal testing which often fails to predict human trial outcomes due species differences. Coupled with ethical concerns around testing, this leads high demand for improved in vitro cell culture platforms. Current efforts, regard, however, are facing challenge provide physiologically relevant organ models reliable assessment of the physiological responses body drug compounds toxins. The latest development models, organ-on-chips (OOCs), seek introduce more realistic function. OOCs use commercial porous polymeric membranes as barrier membrane challenging poor replication architectures. Better recapitulation native basement (BM) characteristics desirable modelling physical (e.g. intestine, skin lung) metabolic liver) models. In review, relevance mechanical properties system behaviour elucidated. Key parameters replicating BM also described. This review provides information future focusing BM-mimicking substrates core structure.

Язык: Английский

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Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Апрель 27, 2023

Microfluidics is an interdisciplinary field that encompasses both science and engineering, which aims to design fabricate devices capable of manipulating extremely low volumes fluids on a microscale level. The central objective microfluidics provide high precision accuracy while using minimal reagents equipment. benefits this approach include greater control over experimental conditions, faster analysis, improved reproducibility. Microfluidic devices, also known as labs-on-a-chip (LOCs), have emerged potential instruments for optimizing operations decreasing costs in various industries, including pharmaceutical, medical, food, cosmetics. However, the price conventional prototypes LOCs generated clean room facilities, has increased demand inexpensive alternatives. Polymers, paper, hydrogels are some materials can be utilized create microfluidic covered article. In addition, we highlighted different manufacturing techniques, such soft lithography, laser plotting, 3D printing, suitable creating LOCs. selection fabrication techniques will depend specific requirements applications each individual LOC. This article comprehensive overview numerous alternatives development low-cost service industries pharmaceuticals, chemicals, biomedicine.

Язык: Английский

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