An overview of in vitro 3D models of the blood-brain barrier as a tool to predict the in vivo permeability of nanomedicines

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 196, С. 114816 - 114816

Опубликована: Март 30, 2023

The blood-brain barrier (BBB) prevents efficient drug delivery to the central nervous system. As a result, brain diseases remain one of greatest unmet medical needs. Understanding tridimensional structure BBB helps gain insight into pathology and contributes development novel therapies for diseases. Therefore, 3D models with an ever-growing sophisticated complexity are being developed closely mimic human neurovascular unit. Among these models, hydrogel-, spheroid- organoid-based static have been developed, so microfluidic-based BBB-on-a-chip models. different preclinical BBB, both in health disease, here reviewed, from their application permeability testing nanomedicines across discussing advantages disadvantages each model. validation data vivo is also discussed those cases where provided.

Язык: Английский

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Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Biomacromolecules, Год журнала: 2024, Номер 25(4), С. 2156 - 2221

Опубликована: Март 20, 2024

Tissue engineering for injured tissue replacement and regeneration has been a subject of investigation over the last 30 years, there considerable interest in using additive manufacturing to achieve these goals. Despite such efforts, many key questions remain unanswered, particularly area biomaterial selection applications as well quantitative understanding process science. The strategic utilization biological macromolecules provides versatile approach meet diverse requirements 3D printing, printability, buildability, biocompatibility. These molecules play pivotal role both physical chemical cross-linking processes throughout biofabrication, contributing significantly overall success printing process. Among several bioprintable materials, gelatin methacryloyl (GelMA) widely utilized applications, with some degree success. In this context, review will discuss bioengineering approaches identify gelation strategies that are appropriate control rheology, buildability inks. This focus on GelMA structural (scaffold) different tissues potential carrier vehicle transport living cells their maintenance viability physiological system. Recognizing importance printability toward shape fidelity biophysical properties, major qualitative impact factors, including microrheological, viscoelastic, gelation, shear thinning properties inks, parameters, particular, reference extrusion GelMA-based Specifically, we emphasize possibilities regulate mechanical, swelling, biodegradation, cellular functionalities bio(material) by hybridization techniques, synthetic natural biopolymers, inorganic nanofillers, microcarriers. At close, possibility integration experimental data sets artificial intelligence/machine learning is emphasized predict fidelity, or inks clinically relevant tissues.

Язык: Английский

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3D Bioprinted Scaffolds for Tissue Repair and Regeneration

Frontiers in Materials, Год журнала: 2022, Номер 9

Опубликована: Июль 7, 2022

Three-dimensional (3D) printing technology has emerged as a revolutionary manufacturing strategy that could realize rapid prototyping and customization. It revolutionized the process in fields of electronics, energy, bioengineering sensing. Based on digital model files, powdered metal, plastic other materials were used to construct required objects by layer layer. In addition, 3D possesses remarkable advantages realizing controllable compositions complex structures, which further produce with anisotropic functions. recent years, bioprinting been applied manufacture functional tissue engineering scaffolds its ability assemble complicated construction under precise control, attracted great attention. Bioprinting creates depositing assembling biological and/or non-biological an established tissue. Compared traditional technology, it can create structure tailored patient according medical images. This conception draws be utilized personalized implants, thereby opening up new way for bio-manufacturing methods. As promising tool, delicate biomimetic simulating extracellular matrix preparing high precision multifunctional uniform cell distribution repair regeneration. also flexibly combined technologies such electrospinning thermally induced phase separation, suitable article reviews relevant research progress regeneration years. Firstly, we will introduce physical, chemical characteristics prepared from several aspects. Secondly, significant effects nerves, skin, blood vessels, bones cartilage injury are expounded. Finally, some views clinical challenges future opportunities put forward.

Язык: Английский

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Synergistic coupling between 3D bioprinting and vascularization strategies

Biofabrication, Год журнала: 2023, Номер 16(1), С. 012003 - 012003

Опубликована: Ноя. 9, 2023

Abstract Three-dimensional (3D) bioprinting offers promising solutions to the complex challenge of vascularization in biofabrication, thereby enhancing prospects for clinical translation engineered tissues and organs. While existing reviews have touched upon 3D vascularized tissue contexts, current review a more holistic perspective, encompassing recent technical advancements spanning entire multistage process, with particular emphasis on vascularization. The synergy between strategies is crucial, as can enable creation personalized, tissue-specific vascular network while enhances viability function. starts by providing comprehensive overview from pre-bioprinting stages post-printing processing, including perfusion maturation. Next, that be seamlessly integrated are discussed. Further, examples illustrating how these approaches customized diverse anatomical towards relevance Finally, underexplored intraoperative (IOB) was highlighted, which enables direct reconstruction within defect sites, stressing possible shaped combining IOB improved regeneration.

Язык: Английский

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Hyaluronic Acid Role in Biomaterials Prevascularization

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract Tissue vascularization is a major bottleneck in tissue engineering. In this review, the state of art on intricate role hyaluronic acid (HA) angiogenesis explored. HA plays twofold angiogenesis. First, when released as free polymer extracellular matrix (ECM), acts signaling molecule triggering multiple cascades that foster smooth muscle cell differentiation, migration, and proliferation thereby contributing to vessel wall thickening. Simultaneously, bound plasma membrane pericellular space functions block, participating formation. Starting with origins native vascular tissues, approaches aimed at achieving vivo are reviewed. The significance molecular weight (MW) challenges associated utilizing engineering (VTE) conscientiously addressed. review finally focuses thorough examination comparison diverse strategies adopted harness benefits bioengineered materials. By providing nuanced perspective multifaceted angiogenesis, contributes ongoing discourse advances collective understanding optimizing processes assisted by functional biomaterials.

Язык: Английский

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Integrating Microfluidics, Hydrogels, and 3D Bioprinting for Personalized Vessel-on-a-Chip Platforms

Biomaterials Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Advancement of vascular models from simple 2D culture to complex vessel-on-a-chip platforms through integration microfluidics, biomimetic hydrogels, and 3D bioprinting, enabling controlled investigation thrombosis mechanisms.

Язык: Английский

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An insight on advances and applications of 3d bioprinting: A review

Bioprinting, Год журнала: 2021, Номер 24, С. e00176 - e00176

Опубликована: Окт. 22, 2021

Язык: Английский

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Modulation of bioactive calcium phosphate micro/nanoparticle size and shape during in situ synthesis of photo-crosslinkable gelatin methacryloyl based nanocomposite hydrogels for 3D bioprinting and tissue engineering

Biomaterials Research, Год журнала: 2022, Номер 26(1)

Опубликована: Сен. 30, 2022

The gelatin-methacryloyl (GelMA) polymer suffers shape fidelity and structural stability issues during 3D bioprinting for bone tissue engineering while homogeneous mixing of reinforcing nanoparticles is always under debate. In this study, amorphous calcium phosphates micro/nanoparticles (CNP) incorporated GelMA synthesized by developing specific sites gelatin structure-based nucleation stabilization in a one-pot processing. process ensures homogenous distribution CNPs different concentrations control their growth morphologies. After synthesis the matrix, methacrylation carried out to prepare homogeneously distributed CNP-reinforced methacryloyl (CNP GelMA) polymer. CNP gel, properties photo-crosslinked scaffolds were compared with those conventionally fabricated ones. (spindle spherical) size (1.753 μm 296 nm) matrix are modulated adjusting synthesis. UV cross-linked (using Irgacure 2955) has significantly improved mechanical (three times compressive strength), printability (160 layers, 2 cm self-standing printed height) biological (cell supportiveness osteogenic differentiation). photo-crosslinking becomes faster due better methacrylation, facilitating continuous or printing. For using like photo cross-linkable polymers, where major concerns, beneficial even regeneration within short period.

Язык: Английский

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3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations

Frontiers in Cardiovascular Medicine, Год журнала: 2022, Номер 9

Опубликована: Март 4, 2022

Despite the efforts devoted to drug discovery and development, number of new approvals have been decreasing. Specifically, cardiovascular developments showing amongst lowest levels approvals. In addition, concerns over adverse effects drugs system increasing resulting in failure at preclinical level as well withdrawal post-marketing. Besides factors such increased cost clinical trials increases requirements complexity regulatory processes, there is also a gap between currently existing pre-clinical screening methods studies humans. This mainly caused by lack used 2D cell culture-based systems, which do not accurately reflect human physiological conditions. Cell-based widely accepted extensively can provide an initial indication drugs' therapeutic efficacy potential cytotoxicity. However, vitro cell-based evaluation could many instances contradictory findings vivo testing animal models trials. drawback related these culture systems recapitulate microenvironment cells reside. body, reside within complex setting, where they interact with respond neighboring cells, extracellular matrix, mechanical stress, blood shear other factors. These sum affect cellular response specific pathways that regulate variable vital functions proliferation, apoptosis, differentiation. Although this complexity, cross species differences cause results from seen when enters Thus, need better mimic conditions improve efficiency screening. A novel approach develop 3D tissue engineered miniaturized constructs are based on cells. review, we discuss should be considered produce successful vascular construct derived both reliable reproducible.

Язык: Английский

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Development and systematic characterization of GelMA/alginate/PEGDMA/xanthan gum hydrogel bioink system for extrusion bioprinting

Biomaterials, Год журнала: 2022, Номер 293, С. 121969 - 121969

Опубликована: Дек. 15, 2022

Язык: Английский

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