Advances in microfabrication technologies in tissue engineering and regenerative medicine

Artificial Organs, Год журнала: 2022, Номер 46(7)

Опубликована: Март 29, 2022

Abstract Background Tissue engineering provides various strategies to fabricate an appropriate microenvironment support the repair and regeneration of lost or damaged tissues. In this matter, several technologies have been implemented construct close‐to‐native three‐dimensional structures at numerous physiological scales, which are essential confer functional characteristics living Methods article, we review a variety microfabrication that currently utilized for tissue applications, such as soft lithography, microneedles, templated self‐assembly microstructures, microfluidics, fiber spinning, bioprinting. Results These considerably helped us precisely manipulate cells cellular constructs fabrication biomimetic tissues organs. Although available still lack some crucial functionalities, including vascular networks, innervation, lymphatic system, being proposed overcome these issues. Moreover, techniques progressed preclinical stage also discussed. Conclusions This article aims highlight advantages drawbacks each technique areas further research more comprehensive evolving understanding in terms regenerative medicine applications.

Язык: Английский

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Characterization and assessment of new fibrillar collagen inks and bioinks for 3D printing and bioprinting

International Journal of Bioprinting, Год журнала: 2023, Номер 9(3), С. 712 - 712

Опубликована: Март 16, 2023

Collagen is a cornerstone protein for tissue engineering and 3D bioprinting due to its outstanding biocompatibility, low immunogenicity, natural abundance in human tissues. Nonetheless, it still poses some important challenges, such as complicated limited extraction processes, usually accompanied by batch- to-batch reproducibility influence of factors, temperature, pH, ionic strength. In this work, we evaluated the suitability performance new, fibrillar type I collagen standardized reproducible source printing bioprinting. The acidic, native fibrous formulation (5% w/w) performed remarkably during printing, which was possible print constructs up 27 layers without collapsing. On other hand, mass has been modified provide fast, reliable, easily neutralizable process. neutralization with TRIS-HCl enabled inclusion cells hindering printability. cell-laden were printed under mild conditions (50-80 kPa, pneumatic printing), providing remarkable cellular viability (>90%) well stable platform cell growth proliferation vitro. Therefore, native, masses characterized work offer reliable purposes.

Язык: Английский

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Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling

BioChip Journal, Год журнала: 2024, Номер 18(3), С. 345 - 356

Опубликована: Май 14, 2024

Abstract The vascular system, essential for human physiology, is vital transporting nutrients, oxygen, and waste. Since structures are involved in various disease pathogeneses exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific models. While animal models possess sophisticated morphologies, they significant discrepancies from tissues due species differences, which limits their applicability. To overcome limitations arising these oversimplification of 2D dish cultures, microphysiological systems (MPS) emerged as a promising alternative. These more accurately mimic microenvironment by incorporating cell interactions, physical stimuli, extracellular matrix components, thus facilitating enhanced tissue differentiation functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model patient responses. This review focuses recent advancements MPS, particularly modeling discusses potential biological adaptation.

Язык: Английский

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Granular Biphasic Colloidal Hydrogels for 3D Bioprinting

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Май 15, 2024

Granular hydrogels composed of hydrogel microparticles are promising candidates for 3D bioprinting due to their ability protect encapsulated cells. However, achieve high print fidelity, need jam exhibit shear-thinning characteristics, which is crucial printing. Unfortunately, this overpacking can significantly impact cell viability, thereby negating the primary advantage using shield cells from shear forces. To overcome challenge, a novel solution: biphasic, granular colloidal bioink designed optimize viability and printing fidelity introduced. The biphasic ink consists cell-laden polyethylene glycol (PEG) embedded in continuous gelatin methacryloyl (GelMA)-nanosilicate network. Here, it demonstrated that offers outstanding rheological properties, structural stability. Furthermore, its utility engineering complex tissues with multiple types heterogeneous microenvironments demonstrated, by incorporating β-islet into PEG endothelial GelMA-nanosilicate Using approach, possible induce patterning, enhance vascularization, direct cellular function. proposed holds significant potential numerous emerging biomedical applications, including tissue disease modeling.

Язык: Английский

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Ink-structing the future of vascular tissue engineering: a review of the physiological bioink design

Bio-Design and Manufacturing, Год журнала: 2024, Номер 7(2), С. 181 - 205

Опубликована: Март 1, 2024

Abstract Three-dimensional (3D) printing and bioprinting have come into view for a plannable standardizable generation of implantable tissue-engineered constructs that can substitute native tissues organs. These structures are intended to integrate with the patient’s body. Vascular tissue engineering (TE) is relevant in TE because it supports sustained oxygenization nutrition all constructs. Bioinks specific role, representing necessary medium printability vascular cell growth. This review aims understand requirements design bioinks. First, an in-depth analysis interaction their environment must be gained. A physiological bioink suitable graft (TEVG) not only ensure good but also induce cells behave like vessel, including self-regenerative growth functions. describes general structure walls wall-specific extracellular matrix (ECM) components biomechanical properties Furthermore, role ECM mode introduced. Diverse currently available or imaginable bioinks described from proteins nonphysiologically occurring natural chemical compounds useful bioprinting. The performance these evaluated regard postprinting, current animal studies 3D printed structures. Finally, main challenges further development, create self-assembly concept, future strategies outlined. concepts discussed terms suitability part TEVG high potential later clinical use.

Язык: Английский

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Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Advances, challenges and future applications of liver organoids in experimental regenerative medicine

Frontiers in Medicine, Год журнала: 2025, Номер 11

Опубликована: Янв. 24, 2025

The liver is a vital organ responsible for numerous metabolic processes in the human body, including metabolism of drugs and nutrients. After damage, can rapidly return to its original size if causative factor promptly eliminated. However, when harmful stimulus persists, liver’s regenerative capacity becomes compromised. Substantial theoretical feasibility has been demonstrated at levels gene expression, molecular interactions, intercellular dynamics, complemented by successful animal studies. robust model carrier that closely resemble physiology are still lacking translating these theories into practice. potential regeneration central focus ongoing research. Over past decade, advent organoid technology provided improved models materials advancing research efforts. Liver represents novel vitro culture system. several years refinement, organoids now accurately replicate morphological structure, nutrient drug metabolism, secretory functions, providing disease Regenerative medicine aims or tissue functions repair replace damaged tissues, restore their structure function, stimulate tissues organs within body. possess same function as tissue, offering serve viable replacement liver, aligning with goals medicine. This review examines role

Язык: Английский

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Clay Minerals as Bioink Ingredients for 3D Printing and 3D Bioprinting: Application in Tissue Engineering and Regenerative Medicine

Pharmaceutics, Год журнала: 2021, Номер 13(11), С. 1806 - 1806

Опубликована: Окт. 28, 2021

The adaptation and progress of 3D printing technology toward bioprinting (specifically adapted to biomedical purposes) has opened the door a world new opportunities possibilities in tissue engineering regenerative medicine. In this regard, allows for production tailor-made constructs organs as well custom implants medical devices. As it is growing field study, currently, attention heeded on optimization improvement mechanical biological properties so-called bioinks/biomaterial inks. One strategies proposed use inorganic ingredients (clays, hydroxyapatite, graphene, carbon nanotubes other silicate nanoparticles). Clays have proven be useful rheological reinforcement wide range fields, from building industry pharmacy. Moreover, they are naturally occurring materials with recognized biocompatibility bioactivity, revealing them optimal candidates cutting-edge technology. This review deals clays (both natural synthetic) medicine through bioprinting. Despite limited number studies, possible conclude that play fundamental role formulation bioinks biomaterial inks since able improve their rheology properties, thus improving printability construct resistance. Additionally, also exceptionally functional (enhancing cellular proliferation, adhesion, differentiation alignment), controlling biodegradation carrying/releasing actives regeneration therapeutic activities.

Язык: Английский

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Bioprinted vascular tissue: Assessing functions from cellular, tissue to organ levels

Materials Today Bio, Год журнала: 2023, Номер 23, С. 100846 - 100846

Опубликована: Окт. 28, 2023

3D bioprinting technology is widely used to fabricate various tissue structures. However, the absence of vessels hampers ability bioprinted tissues receive oxygen and nutrients as well remove wastes, leading a significant reduction in their survival rate. Despite advancements bioinks technologies, vascular structures continue be unsuitable for transplantation compared natural blood vessels. In addition, complete assessment index system evaluating structure function vitro has not yet been established. Therefore, this review, we firstly highlight significance selecting suitable techniques they two synergize with each other. Subsequently, focusing on both vascular-associated cells tissues, provide relatively thorough functions based physiological that possess. We end review applications models, such vessel-on-a-chip, simulating pathological processes conducting drug screening at organ level. believe development fully functional will soon make great contributions engineering regenerative medicine.

Язык: Английский

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Three-Dimensional Bioprinting in Cardiovascular Disease: Current Status and Future Directions

Biomolecules, Год журнала: 2023, Номер 13(8), С. 1180 - 1180

Опубликована: Июль 28, 2023

Three-dimensional (3D) printing plays an important role in cardiovascular disease through the use of personalised models that replicate normal anatomy and its pathology with high accuracy reliability. While 3D printed heart vascular have been shown to improve medical education, preoperative planning simulation cardiac procedures, as well enhance communication patients, bioprinting represents a potential advancement technology by allowing cellular or biological components, functional tissues organs can be used variety applications disease. Recent advances ability support vascularisation large-scale constructs enhanced biocompatibility structural stability, thus creating opportunities replace damaged organs. In this review, we provide overview focus on technologies tissues, grafts, valves myocardium. Limitations future research directions are highlighted.

Язык: Английский

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