3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations

Frontiers in Cardiovascular Medicine, Journal Year: 2022, Volume and Issue: 9

Published: March 4, 2022

Despite the efforts devoted to drug discovery and development, number of new approvals have been decreasing. Specifically, cardiovascular developments showing amongst lowest levels approvals. In addition, concerns over adverse effects drugs system increasing resulting in failure at preclinical level as well withdrawal post-marketing. Besides factors such increased cost clinical trials increases requirements complexity regulatory processes, there is also a gap between currently existing pre-clinical screening methods studies humans. This mainly caused by lack used 2D cell culture-based systems, which do not accurately reflect human physiological conditions. Cell-based widely accepted extensively can provide an initial indication drugs' therapeutic efficacy potential cytotoxicity. However, vitro cell-based evaluation could many instances contradictory findings vivo testing animal models trials. drawback related these culture systems recapitulate microenvironment cells reside. body, reside within complex setting, where they interact with respond neighboring cells, extracellular matrix, mechanical stress, blood shear other factors. These sum affect cellular response specific pathways that regulate variable vital functions proliferation, apoptosis, differentiation. Although this complexity, cross species differences cause results from seen when enters Thus, need better mimic conditions improve efficiency screening. A novel approach develop 3D tissue engineered miniaturized constructs are based on cells. review, we discuss should be considered produce successful vascular construct derived both reliable reproducible.

Language: Английский

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Advances in microfabrication technologies in tissue engineering and regenerative medicine

Artificial Organs, Journal Year: 2022, Volume and Issue: 46(7)

Published: March 29, 2022

Abstract Background Tissue engineering provides various strategies to fabricate an appropriate microenvironment support the repair and regeneration of lost or damaged tissues. In this matter, several technologies have been implemented construct close‐to‐native three‐dimensional structures at numerous physiological scales, which are essential confer functional characteristics living Methods article, we review a variety microfabrication that currently utilized for tissue applications, such as soft lithography, microneedles, templated self‐assembly microstructures, microfluidics, fiber spinning, bioprinting. Results These considerably helped us precisely manipulate cells cellular constructs fabrication biomimetic tissues organs. Although available still lack some crucial functionalities, including vascular networks, innervation, lymphatic system, being proposed overcome these issues. Moreover, techniques progressed preclinical stage also discussed. Conclusions This article aims highlight advantages drawbacks each technique areas further research more comprehensive evolving understanding in terms regenerative medicine applications.

Language: Английский

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Development and systematic characterization of GelMA/alginate/PEGDMA/xanthan gum hydrogel bioink system for extrusion bioprinting

Biomaterials, Journal Year: 2022, Volume and Issue: 293, P. 121969 - 121969

Published: Dec. 15, 2022

Language: Английский

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Characterization and assessment of new fibrillar collagen inks and bioinks for 3D printing and bioprinting

International Journal of Bioprinting, Journal Year: 2023, Volume and Issue: 9(3), P. 712 - 712

Published: March 16, 2023

Collagen is a cornerstone protein for tissue engineering and 3D bioprinting due to its outstanding biocompatibility, low immunogenicity, natural abundance in human tissues. Nonetheless, it still poses some important challenges, such as complicated limited extraction processes, usually accompanied by batch- to-batch reproducibility influence of factors, temperature, pH, ionic strength. In this work, we evaluated the suitability performance new, fibrillar type I collagen standardized reproducible source printing bioprinting. The acidic, native fibrous formulation (5% w/w) performed remarkably during printing, which was possible print constructs up 27 layers without collapsing. On other hand, mass has been modified provide fast, reliable, easily neutralizable process. neutralization with TRIS-HCl enabled inclusion cells hindering printability. cell-laden were printed under mild conditions (50-80 kPa, pneumatic printing), providing remarkable cellular viability (>90%) well stable platform cell growth proliferation vitro. Therefore, native, masses characterized work offer reliable purposes.

Language: Английский

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Ink-structing the future of vascular tissue engineering: a review of the physiological bioink design

Bio-Design and Manufacturing, Journal Year: 2024, Volume and Issue: 7(2), P. 181 - 205

Published: March 1, 2024

Abstract Three-dimensional (3D) printing and bioprinting have come into view for a plannable standardizable generation of implantable tissue-engineered constructs that can substitute native tissues organs. These structures are intended to integrate with the patient’s body. Vascular tissue engineering (TE) is relevant in TE because it supports sustained oxygenization nutrition all constructs. Bioinks specific role, representing necessary medium printability vascular cell growth. This review aims understand requirements design bioinks. First, an in-depth analysis interaction their environment must be gained. A physiological bioink suitable graft (TEVG) not only ensure good but also induce cells behave like vessel, including self-regenerative growth functions. describes general structure walls wall-specific extracellular matrix (ECM) components biomechanical properties Furthermore, role ECM mode introduced. Diverse currently available or imaginable bioinks described from proteins nonphysiologically occurring natural chemical compounds useful bioprinting. The performance these evaluated regard postprinting, current animal studies 3D printed structures. Finally, main challenges further development, create self-assembly concept, future strategies outlined. concepts discussed terms suitability part TEVG high potential later clinical use.

Language: Английский

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Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling

BioChip Journal, Journal Year: 2024, Volume and Issue: 18(3), P. 345 - 356

Published: May 14, 2024

Abstract The vascular system, essential for human physiology, is vital transporting nutrients, oxygen, and waste. Since structures are involved in various disease pathogeneses exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific models. While animal models possess sophisticated morphologies, they significant discrepancies from tissues due species differences, which limits their applicability. To overcome limitations arising these oversimplification of 2D dish cultures, microphysiological systems (MPS) emerged as a promising alternative. These more accurately mimic microenvironment by incorporating cell interactions, physical stimuli, extracellular matrix components, thus facilitating enhanced tissue differentiation functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model patient responses. This review focuses recent advancements MPS, particularly modeling discusses potential biological adaptation.

Language: Английский

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Clay Minerals as Bioink Ingredients for 3D Printing and 3D Bioprinting: Application in Tissue Engineering and Regenerative Medicine

Pharmaceutics, Journal Year: 2021, Volume and Issue: 13(11), P. 1806 - 1806

Published: Oct. 28, 2021

The adaptation and progress of 3D printing technology toward bioprinting (specifically adapted to biomedical purposes) has opened the door a world new opportunities possibilities in tissue engineering regenerative medicine. In this regard, allows for production tailor-made constructs organs as well custom implants medical devices. As it is growing field study, currently, attention heeded on optimization improvement mechanical biological properties so-called bioinks/biomaterial inks. One strategies proposed use inorganic ingredients (clays, hydroxyapatite, graphene, carbon nanotubes other silicate nanoparticles). Clays have proven be useful rheological reinforcement wide range fields, from building industry pharmacy. Moreover, they are naturally occurring materials with recognized biocompatibility bioactivity, revealing them optimal candidates cutting-edge technology. This review deals clays (both natural synthetic) medicine through bioprinting. Despite limited number studies, possible conclude that play fundamental role formulation bioinks biomaterial inks since able improve their rheology properties, thus improving printability construct resistance. Additionally, also exceptionally functional (enhancing cellular proliferation, adhesion, differentiation alignment), controlling biodegradation carrying/releasing actives regeneration therapeutic activities.

Language: Английский

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Bioprinted vascular tissue: Assessing functions from cellular, tissue to organ levels

Materials Today Bio, Journal Year: 2023, Volume and Issue: 23, P. 100846 - 100846

Published: Oct. 28, 2023

3D bioprinting technology is widely used to fabricate various tissue structures. However, the absence of vessels hampers ability bioprinted tissues receive oxygen and nutrients as well remove wastes, leading a significant reduction in their survival rate. Despite advancements bioinks technologies, vascular structures continue be unsuitable for transplantation compared natural blood vessels. In addition, complete assessment index system evaluating structure function vitro has not yet been established. Therefore, this review, we firstly highlight significance selecting suitable techniques they two synergize with each other. Subsequently, focusing on both vascular-associated cells tissues, provide relatively thorough functions based physiological that possess. We end review applications models, such vessel-on-a-chip, simulating pathological processes conducting drug screening at organ level. believe development fully functional will soon make great contributions engineering regenerative medicine.

Language: Английский

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Three-Dimensional Bioprinting in Cardiovascular Disease: Current Status and Future Directions

Biomolecules, Journal Year: 2023, Volume and Issue: 13(8), P. 1180 - 1180

Published: July 28, 2023

Three-dimensional (3D) printing plays an important role in cardiovascular disease through the use of personalised models that replicate normal anatomy and its pathology with high accuracy reliability. While 3D printed heart vascular have been shown to improve medical education, preoperative planning simulation cardiac procedures, as well enhance communication patients, bioprinting represents a potential advancement technology by allowing cellular or biological components, functional tissues organs can be used variety applications disease. Recent advances ability support vascularisation large-scale constructs enhanced biocompatibility structural stability, thus creating opportunities replace damaged organs. In this review, we provide overview focus on technologies tissues, grafts, valves myocardium. Limitations future research directions are highlighted.

Language: Английский

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Granular Biphasic Colloidal Hydrogels for 3D Bioprinting

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 15, 2024

Granular hydrogels composed of hydrogel microparticles are promising candidates for 3D bioprinting due to their ability protect encapsulated cells. However, achieve high print fidelity, need jam exhibit shear-thinning characteristics, which is crucial printing. Unfortunately, this overpacking can significantly impact cell viability, thereby negating the primary advantage using shield cells from shear forces. To overcome challenge, a novel solution: biphasic, granular colloidal bioink designed optimize viability and printing fidelity introduced. The biphasic ink consists cell-laden polyethylene glycol (PEG) embedded in continuous gelatin methacryloyl (GelMA)-nanosilicate network. Here, it demonstrated that offers outstanding rheological properties, structural stability. Furthermore, its utility engineering complex tissues with multiple types heterogeneous microenvironments demonstrated, by incorporating β-islet into PEG endothelial GelMA-nanosilicate Using approach, possible induce patterning, enhance vascularization, direct cellular function. proposed holds significant potential numerous emerging biomedical applications, including tissue disease modeling.

Language: Английский

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