ACS Nano,
Год журнала:
2024,
Номер
19(1), С. 781 - 802
Опубликована: Дек. 21, 2024
Neutrophil
membrane
vesicles
(NMVs)
have
been
successfully
applied
to
control
the
inflammatory
cascade
after
spinal
cord
injury
(SCI)
by
acting
as
an
factor
decoy
front-load
overall
inflammation
regulatory
window;
however,
mechanisms
which
NMVs
regulate
macrophage
phenotypic
shifts
well
their
outcomes
rarely
reported.
In
this
study,
we
demonstrated
"efferocytosis-like"
effect
of
endocytosed
macrophages,
supplementing
TCA
cycle
intermediate
metabolite
α-KG
promoting
glutamine
metabolism,
in
turn
facilitates
oxidative
phosphorylation
and
inhibits
NF-κB
signaling
pathway
reprogram
macrophages
pro-regenerative
phenotype.
Based
on
these
findings,
a
"Trojan
horse"
composite
fiber
scaffold
was
constructed;
comprised
carboxylated
poly-l-lactic
acid
shell
encapsulated
with
core
loaded
brain-derived
neurotrophic
spatiotemporally
modulate
microenvironment
39.23%
sustainably
promote
nerve
regeneration
85.67%.
vivo
experiments
further
confirmed
NMV-coated
scaffolds
regulation
early
innate
immune
continuous
promotion
regeneration.
This
study
not
only
unravels
mechanism
neutrophil
membrane–macrophage
interactions
but
also
provides
strategy
for
coordinating
reprogramming
following
SCI.
It
is
challenging
to
sufficiently
regulate
endogenous
neuronal
reactive
oxygen
species
(ROS)
production,
reduce
apoptosis,
and
reconstruct
neural
networks
under
spinal
cord
injury
conditions.
Here,
hydrogel
surface
grafting
microsol
electrospinning
are
used
construct
a
composite
biomimetic
scaffold
with
"external-endogenous"
dual
regulation
of
ROS.
The
outer
enhances
local
autophagy
through
responsive
degradation
rapid
release
rapamycin
(≈80%
within
week),
neutralizing
extracellular
ROS
inhibiting
further
reducing
apoptosis.
inner
directional
fibers
continuously
supply
brain-derived
neurotrophic
factors
guide
axonal
growth.
results
in
vitro
co-culturing
show
that
the
oxidative
metabolism
by
approximately
doubles
level,
reduces
60%
apoptosis
induced
stress,
increases
differentiation
stem
cells
into
neuron-like
≈2.5
times.
vivo
levels
≈80%
decrease
formation
scar
tissue.
RNA
sequencing
scaffolds
upregulate
autophagy-associated
proteins,
antioxidase
genes,
growth
proteins.
developed
represents
therapeutic
strategy
achieve
neurofunctional
recovery
programmed
accurate
bidirectional
cascade
response.
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101556 - 101556
Опубликована: Фев. 4, 2025
Spinal
cord
injury
(SCI)
presents
a
formidable
challenge
in
clinical
settings,
resulting
sensory
and
motor
function
loss
imposing
significant
personal
societal
burdens.
However,
owning
to
the
adverse
microenvironment
limited
regenerative
capacity,
achieving
complete
functional
recovery
after
SCI
remains
elusive.
Additionally,
traditional
interventions
including
surgery
medication
have
series
of
limitations
that
restrict
effectiveness
treatment.
Recently,
tissue
engineering
(TE)
has
emerged
as
promising
approach
for
promoting
neural
regeneration
SCI,
which
can
effectively
delivery
drugs
into
site
cells
improve
survival
differential.
Here,
we
outline
main
pathophysiology
events
post
injury,
further
discuss
materials
common
assembly
strategies
used
scaffolds
treatment,
expound
on
latest
advancements
treatment
methods
based
drug
cell
detail,
propose
future
directions
repair
with
TE
highlight
potential
applications.
Materials Today Bio,
Год журнала:
2025,
Номер
32, С. 101753 - 101753
Опубликована: Апрель 10, 2025
The
spinal
cord's
limited
regeneration
is
attributed
to
the
scarcity
of
endogenous
stem
cells
and
a
poor
post-injury
microenvironment
in
adult
mammals.
To
overcome
these
challenges,
we
transplanted
DNA
aptamer
19S
(Apt19S)
sustained-release
decellularized
optic
nerve
(DON)
scaffold
(DON-A)
into
completely
transected
cord
injury
(SCI)
site
rats
investigated
its
effect
on
cell
recruitment
differentiation,
which
subsequently
contributed
situ
SCI
repair.
It
has
been
demonstrated
that
Apt19S
specifically
binds
membrane
receptor
alkaline
phosphatase
highly
expressed
neural
(NSCs)
mesenchymal
(MSCs),
our
study
further
proved
can
simultaneously
recruit
NSCs
MSCs
lesion
SCI.
In
study,
DON-A
promoted
proliferation
early
stage
injury,
followed
by
rapid
neurogenesis
through
revascularization
via
MSCs.
Synaptic
connections
between
corticospinal
tracts
calcitonin
gene-related
peptide
positive
fibers
with
newborn
neurons
confirmed
formation
neuronal
relays
at
site,
improved
rats'
motor
sensory
functions.
This
offers
new
strategy
for
recruiting
both
synergistically
low
self-repair
ability,
holding
high
potential
clinical
translation.
Heliyon,
Год журнала:
2023,
Номер
9(9), С. e19933 - e19933
Опубликована: Сен. 1, 2023
Spinal
cord
injury
(SCI)
is
characterized
by
damage
resulting
in
dysfunction
of
the
spinal
cord.
Hydrogels
are
common
biomaterials
that
play
an
important
role
treatment
SCI.
biocompatible,
and
some
have
electrical
conductivity
compatible
with
tissues.
a
high
drug-carrying
capacity,
allowing
them
to
be
used
for
SCI
through
loading
various
types
active
substances,
drugs,
or
cells.
We
first
discuss
basic
anatomy
physiology
human
briefly
its
treatment.
Then,
we
describe
different
strategies
further
crosslinking
classification
hydrogels
detail
hydrogel
prepared
using
processing
methods
Finally,
analyze
future
applications
limitations
The
development
opens
up
new
possibilities
options
Thus,
our
findings
will
inspire
scholars
related
fields
promote
therapy
Tissue Engineering Part A,
Год журнала:
2023,
Номер
30(11-12), С. 293 - 303
Опубликована: Окт. 17, 2023
Nerve
repair
poses
a
significant
challenge
in
the
field
of
tissue
regeneration.
As
bioengineered
therapeutic
method,
nerve
conduits
have
been
developed
to
address
damaged
repair.
However,
despite
their
remarkable
potential,
it
is
still
challenging
encompass
complex
physiologically
microenvironmental
cues
(both
biophysical
and
biochemical
factors)
synergistically
regulate
stem
cell
differentiation
within
implanted
conduits,
especially
facile
manner.
In
this
study,
neurogenic
conduit
with
self-actuated
ability
has
by
