Biomimetic “Trojan Horse” Fibers Modulate Innate Immunity Cascades for Nerve Regeneration DOI Creative Commons
Jie Wu, Jincheng Tang, Lichen Zhang

и другие.

ACS Nano, Год журнала: 2024, Номер 19(1), С. 781 - 802

Опубликована: Дек. 21, 2024

Neutrophil membrane vesicles (NMVs) have been successfully applied to control the inflammatory cascade after spinal cord injury (SCI) by acting as an factor decoy front-load overall inflammation regulatory window; however, mechanisms which NMVs regulate macrophage phenotypic shifts well their outcomes rarely reported. In this study, we demonstrated "efferocytosis-like" effect of endocytosed macrophages, supplementing TCA cycle intermediate metabolite α-KG promoting glutamine metabolism, in turn facilitates oxidative phosphorylation and inhibits NF-κB signaling pathway reprogram macrophages pro-regenerative phenotype. Based on these findings, a "Trojan horse" composite fiber scaffold was constructed; comprised carboxylated poly-l-lactic acid shell encapsulated with core loaded brain-derived neurotrophic spatiotemporally modulate microenvironment 39.23% sustainably promote nerve regeneration 85.67%. vivo experiments further confirmed NMV-coated scaffolds regulation early innate immune continuous promotion regeneration. This study not only unravels mechanism neutrophil membrane–macrophage interactions but also provides strategy for coordinating reprogramming following SCI.

Язык: Английский

Reactive Oxygen Species‐Responsive Composite Fibers Regulate Oxidative Metabolism through Internal and External Factors to Promote the Recovery of Nerve Function DOI
Hongyi Zhu, Liang Zhou, Jincheng Tang

и другие.

Small, Год журнала: 2024, Номер unknown

Опубликована: Апрель 25, 2024

It is challenging to sufficiently regulate endogenous neuronal reactive oxygen species (ROS) production, reduce apoptosis, and reconstruct neural networks under spinal cord injury conditions. Here, hydrogel surface grafting microsol electrospinning are used construct a composite biomimetic scaffold with "external-endogenous" dual regulation of ROS. The outer enhances local autophagy through responsive degradation rapid release rapamycin (≈80% within week), neutralizing extracellular ROS inhibiting further reducing apoptosis. inner directional fibers continuously supply brain-derived neurotrophic factors guide axonal growth. results in vitro co-culturing show that the oxidative metabolism by approximately doubles level, reduces 60% apoptosis induced stress, increases differentiation stem cells into neuron-like ≈2.5 times. vivo levels ≈80% decrease formation scar tissue. RNA sequencing scaffolds upregulate autophagy-associated proteins, antioxidase genes, growth proteins. developed represents therapeutic strategy achieve neurofunctional recovery programmed accurate bidirectional cascade response.

Язык: Английский

Процитировано

3

Spinal Cord Injury Repair Based on Drug and Cell Delivery: from Remodeling Microenvironment to Relay Connection Formation DOI Creative Commons

Wanrong Ma,

Xing Li

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101556 - 101556

Опубликована: Фев. 4, 2025

Spinal cord injury (SCI) presents a formidable challenge in clinical settings, resulting sensory and motor function loss imposing significant personal societal burdens. However, owning to the adverse microenvironment limited regenerative capacity, achieving complete functional recovery after SCI remains elusive. Additionally, traditional interventions including surgery medication have series of limitations that restrict effectiveness treatment. Recently, tissue engineering (TE) has emerged as promising approach for promoting neural regeneration SCI, which can effectively delivery drugs into site cells improve survival differential. Here, we outline main pathophysiology events post injury, further discuss materials common assembly strategies used scaffolds treatment, expound on latest advancements treatment methods based drug cell detail, propose future directions repair with TE highlight potential applications.

Язык: Английский

Процитировано

0

DON-Apt19S bioactive scaffold transplantation promotes in situ spinal cord repair in rats with transected spinal cord injury by effectively recruiting endogenous neural stem cells and mesenchymal stem cells DOI Creative Commons
Bi‐Qin Lai, Rongjie Wu, Chuangran Wu

и другие.

Materials Today Bio, Год журнала: 2025, Номер 32, С. 101753 - 101753

Опубликована: Апрель 10, 2025

The spinal cord's limited regeneration is attributed to the scarcity of endogenous stem cells and a poor post-injury microenvironment in adult mammals. To overcome these challenges, we transplanted DNA aptamer 19S (Apt19S) sustained-release decellularized optic nerve (DON) scaffold (DON-A) into completely transected cord injury (SCI) site rats investigated its effect on cell recruitment differentiation, which subsequently contributed situ SCI repair. It has been demonstrated that Apt19S specifically binds membrane receptor alkaline phosphatase highly expressed neural (NSCs) mesenchymal (MSCs), our study further proved can simultaneously recruit NSCs MSCs lesion SCI. In study, DON-A promoted proliferation early stage injury, followed by rapid neurogenesis through revascularization via MSCs. Synaptic connections between corticospinal tracts calcitonin gene-related peptide positive fibers with newborn neurons confirmed formation neuronal relays at site, improved rats' motor sensory functions. This offers new strategy for recruiting both synergistically low self-repair ability, holding high potential clinical translation.

Язык: Английский

Процитировано

0

The recruitment of alkaline phosphatase-positive endogenous neural stem cells enables robust neurogenesis and neural pathways reconstruction following spinal cord injury DOI Creative Commons
Rongjie Wu, Jialin Liu, Zhenghong Chen

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162988 - 162988

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Polymer Scaffolds for peripheral nerve injury repair DOI

Shuhang Yang,

Li Chen, Changning Bai

и другие.

Progress in Materials Science, Год журнала: 2025, Номер unknown, С. 101497 - 101497

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Hydrogel-based treatments for spinal cord injuries DOI Creative Commons
Zhiqiang Jia,

Huanxuan Zeng,

Xiuzhi Ye

и другие.

Heliyon, Год журнала: 2023, Номер 9(9), С. e19933 - e19933

Опубликована: Сен. 1, 2023

Spinal cord injury (SCI) is characterized by damage resulting in dysfunction of the spinal cord. Hydrogels are common biomaterials that play an important role treatment SCI. biocompatible, and some have electrical conductivity compatible with tissues. a high drug-carrying capacity, allowing them to be used for SCI through loading various types active substances, drugs, or cells. We first discuss basic anatomy physiology human briefly its treatment. Then, we describe different strategies further crosslinking classification hydrogels detail hydrogel prepared using processing methods Finally, analyze future applications limitations The development opens up new possibilities options Thus, our findings will inspire scholars related fields promote therapy

Язык: Английский

Процитировано

8

4D Printed Nerve Conduit with In Situ Neurogenic Guidance for Nerve Regeneration DOI
Haitao Cui, Wei Zhu, Shida Miao

и другие.

Tissue Engineering Part A, Год журнала: 2023, Номер 30(11-12), С. 293 - 303

Опубликована: Окт. 17, 2023

Nerve repair poses a significant challenge in the field of tissue regeneration. As bioengineered therapeutic method, nerve conduits have been developed to address damaged repair. However, despite their remarkable potential, it is still challenging encompass complex physiologically microenvironmental cues (both biophysical and biochemical factors) synergistically regulate stem cell differentiation within implanted conduits, especially facile manner. In this study, neurogenic conduit with self-actuated ability has by

Язык: Английский

Процитировано

7

Current multi-scale biomaterials for tissue regeneration following spinal cord injury DOI

Y Zhang,

Zhonghuan Wu,

Junfeng Wu

и другие.

Neurochemistry International, Год журнала: 2024, Номер 178, С. 105801 - 105801

Опубликована: Июль 5, 2024

Язык: Английский

Процитировано

2

Bioinspired conductive oriented nanofiber felt with efficient ROS clearance and anti-inflammation for inducing M2 macrophage polarization and accelerating spinal cord injury repair DOI Creative Commons
Qingxia Zhang, Jiahe Zheng, Linlong Li

и другие.

Bioactive Materials, Год журнала: 2024, Номер 46, С. 173 - 194

Опубликована: Дек. 13, 2024

Язык: Английский

Процитировано

2

Effects of Electrospun Nanofibers on Motor Function Recovery After Spinal Cord Injury: A Systematic Review and Meta-Analysis DOI

Niloofar Haeri Moghaddam,

Somayeh Hashamdar,

Michael R. Hamblin

и другие.

World Neurosurgery, Год журнала: 2023, Номер 181, С. 96 - 106

Опубликована: Окт. 17, 2023

Язык: Английский

Процитировано

4