Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 12, 2025
Abstract
Cell–cell
communication
serves
as
a
foundation
for
intercellular
therapeutic
hand‐over.
Despite
the
commonsense
level
of
understanding,
no
clear
projection
has
been
made
to
prove
mechanism.
Here,
hand‐over
aspirin
from
monocytes
inflamed
cells
is
validated
using
high‐resolution
time
series
3D
imaging
in
vitro,
with
vivo
confirmation.
Notably,
caveolin
identified
play
major
role
mediating
cell
receptors
by
super‐resolution
microscopy,
which
induced
overexpression
upon
inflammation.
When
aspirin‐liposomes
are
loaded
into
splenic
monocytes,
they
naturally
target
sites
efficiently
because
spleen
site
liposomal
clearance
body,
addition
monocyte
residence
leave
toward
inflammatory
signals.
The
delivery
efficiency
and
anti‐inflammatory
effects
through
intravenous
injection
superior
those
oral
soluble
aspirin,
confirmed
ischemic
hindlimb
fatty
liver
mice
(targeted
therapy).
These
results
also
agreed
antiplatelet
effect
mouse
blood
over
7
days
(prolonged
therapy)
combination
these
actions
effectively
rescues
atherosclerotic
carotid
arteries
mice.
This
study
demonstrates
working
mechanism
hand‐over,
suggesting
translational
strategy
improve
delivery.
Molecules,
Год журнала:
2024,
Номер
29(13), С. 3131 - 3131
Опубликована: Июль 1, 2024
The
delivery
of
therapeutic
agents
faces
significant
hurdles
posed
by
the
endo-lysosomal
pathway,
a
bottleneck
that
hampers
clinical
effectiveness.
This
comprehensive
review
addresses
urgent
need
to
enhance
cellular
mechanisms
overcome
these
obstacles.
It
focuses
on
potential
smart
nanomaterials,
delving
into
their
unique
characteristics
and
in
detail.
Special
attention
is
given
ability
strategically
evade
endosomal
entrapment,
thereby
enhancing
efficacy.
manuscript
thoroughly
examines
assays
crucial
for
understanding
escape
uptake
dynamics.
By
analyzing
various
assessment
methods,
we
offer
nuanced
insights
investigative
approaches'
multifaceted
aspects.
We
meticulously
analyze
use
nanocarriers,
exploring
diverse
such
as
pore
formation,
proton
sponge
effects,
membrane
destabilization,
photochemical
disruption,
strategic
agents.
Each
mechanism's
effectiveness
application
mitigating
entrapment
are
scrutinized.
paper
provides
critical
overview
current
landscape,
emphasizing
advanced
systems
navigate
complexities
uptake.
Importantly,
it
underscores
transformative
role
nanomaterials
revolutionizing
strategies,
leading
paradigm
shift
towards
improved
outcomes.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(14), С. 7559 - 7559
Опубликована: Июль 10, 2024
This
systematic
review
offers
a
comprehensive
analysis
of
plant-derived
extracellular
vesicles
(PDEVs)
as
emerging
drug
delivery
systems,
focusing
on
original
research
articles
published
between
2016
and
2024
that
exclusively
examine
the
use
PDEVs
for
delivery.
After
rigorous
search
across
multiple
databases,
20
relevant
studies
out
805
initial
results
were
selected
analysis.
systematically
summarizes
critical
data
PDEV
components,
isolation
methods,
drug-loading
techniques.
It
highlights
potential
to
significantly
enhance
safety
efficacy,
reduce
dosage
toxicity,
align
development
with
sustainable
environmentally
friendly
biotechnological
processes.
also
emphasizes
advantages
over
mammalian-derived
vesicles,
such
cost-effectiveness,
higher
yield,
reduced
immunogenicity.
Additionally,
it
explores
synergistic
encapsulated
drugs
bioactive
compounds
naturally
present
in
PDEVs.
study
acknowledges
challenges
standardizing
formulation
methods
clinical
use.
Overall,
this
provides
valuable
insights
into
current
state
future
directions
PDEV-based
highlighting
their
promising
role
advancing
pharmaceutical
development.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(6), С. 3392 - 3392
Опубликована: Март 16, 2024
Photodynamic
therapy
(PDT)
is
a
therapeutic
option
for
cancer,
in
which
photosensitizer
(PS)
drugs,
light,
and
molecular
oxygen
generate
reactive
species
(ROS)
induce
cell
death.
First-
second-generation
PSs
presented
with
problems
that
hindered
their
efficacy,
including
low
solubility.
Thus,
loaded
into
nanocarriers
were
produced
to
enhance
cellular
uptake
efficacy.
Among
other
compounds
investigated,
the
dye
methylene
blue
(MB)
showed
potential
as
PS,
its
photodynamic
activity
tumor
cells
was
reported
even
nanocarrier-delivered
form,
liposomes.
Here,
we
prepared
polydopamine
(PDA)-coated
liposomes
efficiently
adsorbed
MB
onto
surface.
lipoPDA@MB
vesicles
first
physico-chemically
characterized
studies
on
light
stability
vitro
release
of
performed.
effects
then
assessed
panel
2D-
3D-cultured
cancer
lines,
comparing
results
those
obtained
using
free
MB.
uptake,
type
death
induced,
ability
ROS
also
investigated.
Our
show
possesses
higher
potency
compared
both
2D
3D
models,
probably
thanks
production,
apoptotic
induction.
Therefore,
appears
an
efficient
drug
delivery
system
MB-based
PDT.
Molecules,
Год журнала:
2024,
Номер
29(10), С. 2325 - 2325
Опубликована: Май 15, 2024
Thrombotic
disease
has
been
listed
as
the
third
most
fatal
vascular
in
world.
After
decades
of
development,
clinical
thrombolytic
drugs
still
cannot
avoid
occurrence
adverse
reactions
such
bleeding.
A
number
studies
have
shown
that
application
various
nano-functional
materials
thrombus-targeted
drug
delivery,
combined
with
external
stimuli,
magnetic,
near-infrared
light,
ultrasound,
etc.,
enrich
thrombus
site
and
use
properties
for
collaborative
thrombolysis,
which
can
effectively
reduce
bleeding
improve
thrombolysis
efficiency.
In
this
paper,
research
progress
organic
nanomaterials,
inorganic
biomimetic
nanomaterials
delivery
is
briefly
reviewed.
Journal of Functional Biomaterials,
Год журнала:
2024,
Номер
15(6), С. 169 - 169
Опубликована: Июнь 19, 2024
Nanoparticle
(NP)-based
solutions
for
oncotherapy
promise
an
improved
efficiency
of
the
anticancer
response,
as
well
higher
comfort
patient.
The
current
advancements
in
cancer
treatment
based
on
nanotechnology
exploit
ability
these
systems
to
pass
biological
barriers
target
tumor
cell,
cell
organelles.
In
particular,
iron
oxide
NPs
are
being
clinically
employed
oncological
management
due
this
ability.
When
designing
efficient
anti-cancer
therapy
NPs,
it
is
important
know
and
modulate
phenomena
which
take
place
during
interaction
with
cells,
normal
tissues.
regard,
our
review
focused
highlighting
different
approaches
studying
internalization
patterns
simple
complex
2D
3D
vitro
models,
living
tissues,
order
investigate
functionality
NP-based
treatment.
International Journal for Parasitology,
Год журнала:
2024,
Номер
54(12), С. 617 - 634
Опубликована: Июнь 24, 2024
Fasciola
hepatica
and
Dicrocoelium
dendriticum
are
parasitic
trematodes
residing
in
the
bile
ducts
of
mammalian
hosts,
causing,
some
cases,
impairment
liver
function
hepatic
fibrosis.
Previous
studies
have
shown
that
extracellular
vesicles
released
by
F.
(FhEVs)
D.
(DdEVs)
induce
a
distinct
phenotype
human
macrophages,
but
there
is
limited
information
on
effect
EVs
cells,
which
interact
directly
with
worms
natural
infections.
In
this
study,
we
isolated
FhEVs
DdEVs
size
exclusion
chromatography
labeled
them
lipophilic
fluorescent
dye
to
analyze
their
uptake
stellate
cells
(HSC)
hepatocytes,
important
cell
types
pathology,
using
synthetic
liposomes
as
internal
labeling
control.
We
analyzed
EV
proteome
profiles
after
treatment
for
both
types.
Our
results
reveal
establish
unique
specific
interactions
suggesting
different
role
derived
from
each
parasite,
depending
migration
route
reach
final
niche.
cytostatic
HSCs,
matrix
secretion
elicit
anti-inflammatory
responses
hepatocytes.
more
potent
anti-proliferative
than
trigger
global
inflammatory
response,
increasing
levels
NF-κB
other
mediators
These
may
major
influence
progression
disease,
serving
generate
conditions
favor
establishment
helminths
host.
Journal of Extracellular Biology,
Год журнала:
2025,
Номер
4(1)
Опубликована: Янв. 1, 2025
Abstract
Human
milk
extracellular
vesicles
(EVs)
are
crucial
mother‐to‐baby
messengers
that
transfer
biological
signals.
These
EVs
reported
to
survive
digestion
and
transport
across
the
intestine.
The
mechanisms
of
interaction
between
human
intestinal
mucosa,
including
epithelial
uptake
remain
unclear.
Here,
we
studied
with
gut
barrier
components,
biofluids,
enzymes,
mucus
epithelium.
Additionally,
probed
endocytic
mediating
EV
uptake.
Finally,
using
proteomic
analysis,
determined
existence
identification
proteins
enriched
in
fraction
transported
We
show
largely
stable
biochemical
barriers
demonstrate
high
diffusivity.
a
level
cell
(∼70%)
efficient
Caco‐2
monolayers.
Whilst
was
mediated
by
multiple
routes,
none
pathway‐specific
inhibitors
inhibited
their
translocation.
Proteomic
analysis
monolayers
identified
14
may
facilitate
transport.
findings
significantly
expand
our
understanding
interactions
barriers,
