Micromachines,
Год журнала:
2025,
Номер
16(4), С. 429 - 429
Опубликована: Апрель 2, 2025
Scaffolds
play
a
crucial
role
in
tissue
engineering
as
regenerative
templates.
Fabricating
scaffolds
with
good
biocompatibility
and
appropriate
mechanical
properties
remains
major
challenge
this
field.
This
study
proposes
method
for
preparing
multi-material
scaffolds,
enabling
the
3D
printing
of
collagen
thermoplastic
elastomers
at
room
temperature.
Addressing
previous
challenges
such
poor
printability
pure
difficulty
maintaining
structural
integrity
during
multilayer
printing,
research
improved
by
optimizing
its
concentration
pH
value
completed
large-span
elastomer
using
precise
temperature-control
system.
The
developed
hybrid
scaffold
has
an
interconnected
porous
structure,
which
can
support
adhesion
proliferation
fibroblasts.
were
further
treated
different
post-treatment
methods,
it
was
proven
that
neutralized
cross-linked
scaffold,
both
nano-fibers
certain
rigidity,
better
osteogenic
differentiation
bone
marrow
mesenchymal
stem
cells
(BMSCs).
results
show
significant
clinical
application
potential
soft
hard
regeneration,
providing
versatile
solution
to
meet
diverse
needs
engineering.
Theranostics,
Год журнала:
2024,
Номер
14(3), С. 1101 - 1125
Опубликована: Янв. 1, 2024
Cancer
remains
a
severe
public
health
burden
worldwide.One
of
the
challenges
hampering
effective
cancer
therapy
is
that
existing
models
hardly
recapitulate
tumor
microenvironment
human
patients.Over
past
decade,
organoids
have
emerged
as
an
in
vitro
3D
model
to
mimic
pathophysiological
characteristics
parental
tumors.Various
techniques
been
developed
construct
organoids,
such
matrix-based
methods,
hanging
drop,
spinner
or
rotating
flask,
nonadhesive
surface,
organ-on-a-chip,
bioprinting,
and
genetic
engineering.This
review
elaborated
on
cell
components
fabrication
methods
for
establishing
organoid
models.Furthermore,
we
discussed
application
modeling,
basic
research,
anticancer
therapy.Finally,
current
limitations
future
directions
employing
more
extensive
applications.
Bioactive Materials,
Год журнала:
2023,
Номер
32, С. 356 - 384
Опубликована: Окт. 21, 2023
Three-dimensional
bioprinting
is
an
advanced
tissue
fabrication
technique
that
allows
printing
complex
structures
with
precise
positioning
of
multiple
cell
types
layer-by-layer.
Compared
to
other
methods,
extrusion
has
several
advantages
print
large-sized
constructs
and
organ
models
due
large
build
volume.
Extrusion
using
sacrificial,
support
embedded
strategies
have
been
successfully
employed
facilitate
hollow
structures.
Embedded
a
gel-in-gel
approach
developed
overcome
the
gravitational
overhanging
limits
micron-scale
resolution.
In
bioprinting,
deposition
bioinks
into
microgel
or
granular
bath
will
be
facilitated
by
sol-gel
transition
through
needle
movement
inside
medium.
This
review
outlines
various
polymers
used
in
systems
advantages,
limitations,
efficacy
vascularized
tissues
Further,
essential
requirements
like
viscoelasticity,
stability,
transparency
easy
extraction
human
scale
organs
are
discussed.
Additionally,
geometries
vascular
constructs,
heart,
bone,
octopus
jellyfish
printed
assisted
methods
their
anatomical
features
elaborated.
Finally,
challenges
clinical
translation
future
scope
these
replace
native
envisaged.
Biofabrication,
Год журнала:
2024,
Номер
16(3), С. 032006 - 032006
Опубликована: Май 2, 2024
Abstract
Organoids
have
emerged
as
crucial
platforms
in
tissue
engineering
and
regenerative
medicine
but
confront
challenges
faithfully
mimicking
native
structures
functions.
Bioprinting
technologies
offer
a
significant
advancement,
especially
when
combined
with
organoid
bioinks-engineered
formulations
designed
to
encapsulate
both
the
architectural
functional
elements
of
specific
tissues.
This
review
provides
rigorous,
focused
examination
evolution
impact
bioprinting.
It
emphasizes
role
bioinks
that
integrate
key
cellular
components
microenvironmental
cues
more
accurately
replicate
complexity.
Furthermore,
this
anticipates
transformative
landscape
invigorated
by
integration
artificial
intelligence
bioprinting
techniques.
Such
fusion
promises
refine
bioink
optimize
parameters,
thus
catalyzing
unprecedented
advancements
medicine.
In
summary,
accentuates
pivotal
potential
advancing
therapies,
deepening
our
understanding
organ
development,
clarifying
disease
mechanisms.
Advances in Polymer Technology,
Год журнала:
2024,
Номер
2024(1)
Опубликована: Янв. 1, 2024
Poly(N‐isopropylacrylamide)
(PNIPAM)
is
a
versatile
polymer
known
for
its
phase
transition
properties,
exhibiting
lower
critical
solution
temperature
(LCST)
of
approximately
32°C.
Below
this
temperature,
PNIPAM
hydrophilic,
while
above
it,
the
becomes
hydrophobic,
making
it
ideal
thermosensitive
drug
delivery
systems
(DDSs).
In
tissue
engineering,
provides
biocompatible,
nontoxic
and
stimuli‐responsive
surface
cell
culture.
Its
nature
ensures
safety
in
medical
applications.
enhances
biosensing
diagnostics
through
affinity
biomolecules,
improving
accuracy.
Widely
used
hydrogels,
smart
textiles,
soft
robotics
various
applications,
adapts
to
environmental
changes.
straightforward
synthesis
allows
creation
diverse
copolymers
composites,
applicable
selective
reactions
conjugations
with
fluorescent
tags
or
chemical
modifications.
PNIPAM’s
versatility
extends
pH‐responsive
alternatives,
broadening
application
spectrum.
Practical
examples
include
separation
water
treatment
cleaning
processes.
This
discussion
explores
biomedical
particularly
cancer
treatment,
photothermal
therapy
(PTT)
photodynamic
(PDT),
gene
imaging.
Additionally,
highlights
noncancerous
such
as
small
interfering
RNA
(siRNA)
targeting
oncogenes
detailed
imaging
deep
tumour
tissues.
Biofabrication,
Год журнала:
2024,
Номер
16(3), С. 035026 - 035026
Опубликована: Май 29, 2024
The
evaluation
of
anti-tumor
drugs
is
critical
for
their
development
and
clinical
guidance.
Tumor
organoid
models
are
gaining
increased
attention
due
to
ability
better
mimic
real
tumor
tissues,
as
well
lower
time
economic
costs,
which
makes
up
the
shortcomings
cell
lines
xenograft
models.
However,
current
cultures
based
on
Matrigel
have
limitations
in
matching
with
high-throughput
engineering
methods
slow
gelation
low
mechanical
strength.
Here,
we
present
a
novel
composite
bioink
culturing
colorectal
cancer
organoids
that
provides
an
environment
close
tissue
growth
conditions
exhibits
excellent
photocrosslinking
properties
rapid
gel
formation.
Most
importantly,
viability
after
printing
was
high
97%,
also
kept
multicellular
polar
structures
consistent
traditional
culture
Matrigel.
Using
3D
bioprinting
this
loaded
organoids,
demonstrated
feasibility
drug
model
by
validating
it
clinically
used
treatment
drugs.
Our
results
suggested
could
effectively
cultivate
using
bioprinting,
had
potential
replace
less
reliable
manual
operations
promoting
application
Three-dimensional
(3D)
printing,
also
known
as
additive
manufacturing,
has
revolutionized
the
production
of
physical
3D
objects
by
transforming
computer-aided
design
models
into
layered
structures,
eliminating
need
for
traditional
molding
or
machining
techniques.
In
recent
years,
hydrogels
have
emerged
an
ideal
printing
feedstock
material
fabrication
hydrated
constructs
that
replicate
extracellular
matrix
found
in
endogenous
tissues.
Hydrogels
seen
significant
advancements
since
their
first
use
contact
lenses
biomedical
field.
These
led
to
development
complex
3D-printed
structures
include
a
wide
variety
organic
and
inorganic
materials,
cells,
bioactive
substances.
The
most
commonly
used
techniques
fabricate
hydrogel
scaffolds
are
extrusion,
jetting,
vat
photopolymerization,
but
novel
methods
can
enhance
resolution
structural
complexity
printed
emerged.
applications
be
broadly
classified
four
categories—tissue
engineering
regenerative
medicine,
cell
culture
disease
modeling,
drug
screening
toxicity
testing,
devices
delivery
systems.
Despite
applications,
number
challenges
still
addressed
maximize
printing.
improving
complexity,
optimizing
viability
function,
cost
efficiency
accessibility,
addressing
ethical
regulatory
concerns
clinical
translation.
