Advanced Materials,
Год журнала:
2020,
Номер
32(24)
Опубликована: Апрель 29, 2020
Abstract
Recently
developed
stem‐cell‐based
in
vitro
models
of
morphogenesis
can
help
shed
light
on
the
mechanisms
involved
embryonic
patterning.
These
are
showcased
using
traditional
cell
culture
platforms
and
materials,
which
allow
limited
control
over
biological
system
usually
do
not
support
high‐content
imaging.
In
contrast,
advanced
microengineered
tools
microscale
control,
long‐term
culture,
real‐time
data
acquisition
from
such
aid
elucidating
underlying
mechanisms.
Here,
a
new
culturing,
manipulation
analysis
platform
is
described
to
study
thin
polycarbonate
film‐based
microdevices.
A
pipeline
consisting
open‐source
software
quantify
3D
movement
4D
image
analyze
migration
within
multicellular
clusters.
It
shown
that
be
used
non‐adherent
cultures
P19C5
mouse
stem
line
cells
(mESCs)
show
symmetry
breaking
axial
elongation
events
similar
early
development.
Using
platform,
it
found
localized
proliferation
coordinated
result
aggregates.
Further,
polarization
mESC
aggregates
dependent
directed
migration.
Frontiers in Cell and Developmental Biology,
Год журнала:
2021,
Номер
9
Опубликована: Ноя. 26, 2021
Limited
by
the
poor
proliferation
and
restricted
sources
of
adult
hepatocytes,
there
is
an
urgent
need
to
find
substitutes
for
cultivation
mature
hepatocytes
in
vitro
use
disease
treatment,
drug
approval,
toxicity
testing.
Hepatocyte-like
cells
(HLCs),
which
originate
from
undifferentiated
stem
or
modified
cells,
are
considered
good
candidates
because
their
advantages
terms
cell
source
expansion
ability.
However,
majority
induced
HLCs
immature
state,
degree
differentiation
heterogeneous,
diminishing
usability
basic
research
limiting
clinical
application.
Therefore,
various
methods
have
been
developed
promote
maturation
HLCs,
including
chemical
approaches,
alteration
culture
systems,
genetic
manipulation,
meet
needs
vivo
transplantation
model
establishment.
This
review
proposes
different
types
induction
provide
a
comprehensive
overview
techniques
generation
.
Tissue Engineering Part A,
Год журнала:
2019,
Номер
25(19-20), С. 1341 - 1346
Опубликована: Авг. 14, 2019
Cells
are
inherently
conferred
with
the
ability
to
self-organize
into
tissues
and
organs
comprising
human
body.
Self-organization
can
be
recapitulated
in
vitro
recent
advances
organoid
field
just
one
example
of
how
we
generate
small
functioning
elements
organs.
Tissue
engineers
benefit
from
power
self-organization
should
consider
they
harness
enhance
process
their
constructs.
For
example,
aggregates
stem
cells
tissue-specific
input
carefully
selected
biomolecules
guide
differentiation
toward
a
mature
phenotype.
This
further
enhanced
by
use
technologies
provide
physiological
microenvironment
for
self-organization,
size
constructs,
enable
long-term
culture
self-organized
structures.
Of
importance,
conducting
limited
fine-tuning
avoid
over-engineering
that
could
counteract
inherent
cellular
self-organization.
is
powerful
innate
feature
fine-tuned
but
not
over-engineered
create
new
Advanced Materials,
Год журнала:
2020,
Номер
32(24)
Опубликована: Апрель 29, 2020
Abstract
Recently
developed
stem‐cell‐based
in
vitro
models
of
morphogenesis
can
help
shed
light
on
the
mechanisms
involved
embryonic
patterning.
These
are
showcased
using
traditional
cell
culture
platforms
and
materials,
which
allow
limited
control
over
biological
system
usually
do
not
support
high‐content
imaging.
In
contrast,
advanced
microengineered
tools
microscale
control,
long‐term
culture,
real‐time
data
acquisition
from
such
aid
elucidating
underlying
mechanisms.
Here,
a
new
culturing,
manipulation
analysis
platform
is
described
to
study
thin
polycarbonate
film‐based
microdevices.
A
pipeline
consisting
open‐source
software
quantify
3D
movement
4D
image
analyze
migration
within
multicellular
clusters.
It
shown
that
be
used
non‐adherent
cultures
P19C5
mouse
stem
line
cells
(mESCs)
show
symmetry
breaking
axial
elongation
events
similar
early
development.
Using
platform,
it
found
localized
proliferation
coordinated
result
aggregates.
Further,
polarization
mESC
aggregates
dependent
directed
migration.
