Collective behaviours in organoids DOI Creative Commons
Qiutan Yang, Prisca Liberali

Current Opinion in Cell Biology, Journal Year: 2021, Volume and Issue: 72, P. 81 - 90

Published: July 28, 2021

Collective behaviour emerges from interacting units within communities, such as migrating herds, swimming fish schools, and cells tissues. At the microscopic level, collective behaviours include cell migration in development cancer invasion, rhythmic gene expression pattern formation, competition homeostasis cancer, force generation mechano-sensing morphogenesis. Studying initiation maintenance of is key to understand principles development, regeneration disease. However, manifold influences contributing factors vivo environments challenge dissection causalities animal models. As an alternative model that has emerged overcome this difficulty, vitro three-dimensional organoid cultures provide a reductionist approach yet retain similarities with tissue cellular composition organisation. Here, we focus on recent progresses studying different systems discuss their advantages possibility improvement for future applications.

Language: Английский

Building Complex Life Through Self-Organization DOI
Mireille M.J.P.E. Sthijns, Vanessa L.S. LaPointe, Clemens van Blitterswijk

et al.

Tissue Engineering Part A, Journal Year: 2019, Volume and Issue: 25(19-20), P. 1341 - 1346

Published: Aug. 14, 2019

Cells are inherently conferred with the ability to self-organize into tissues and organs comprising human body. Self-organization can be recapitulated in vitro recent advances organoid field just one example of how we generate small functioning elements organs. Tissue engineers benefit from power self-organization should consider they harness enhance process their constructs. For example, aggregates stem cells tissue-specific input carefully selected biomolecules guide differentiation toward a mature phenotype. This further enhanced by use technologies provide physiological microenvironment for self-organization, size constructs, enable long-term culture self-organized structures. Of importance, conducting limited fine-tuning avoid over-engineering that could counteract inherent cellular self-organization. is powerful innate feature fine-tuned but not over-engineered create new

Language: Английский

Citations

22
Direct ink writing combined with metal-assisted chemical etching of microchannels for the microfluidic system applications DOI
Yong Peng, Shulan Jiang, Li Xia

et al.

Sensors and Actuators A Physical, Journal Year: 2020, Volume and Issue: 315, P. 112320 - 112320

Published: Sept. 6, 2020

Language: Английский

Citations

20
Microfluidic technologies to engineer mesenchymal stem cell aggregates—applications and benefits DOI
Seyedeh Sarah Salehi,

Amir Shamloo,

Siamak Kazemzadeh Hannani

et al.

Biophysical Reviews, Journal Year: 2020, Volume and Issue: 12(1), P. 123 - 133

Published: Jan. 17, 2020

Language: Английский

Citations

19
A New Microengineered Platform for 4D Tracking of Single Cells in a Stem‐Cell‐Based In Vitro Morphogenesis Model DOI Creative Commons
Pinak Samal, Philipp Maurer, Clemens van Blitterswijk

et al.

Advanced Materials, Journal Year: 2020, Volume and Issue: 32(24)

Published: April 29, 2020

Abstract Recently developed stem‐cell‐based in vitro models of morphogenesis can help shed light on the mechanisms involved embryonic patterning. These are showcased using traditional cell culture platforms and materials, which allow limited control over biological system usually do not support high‐content imaging. In contrast, advanced microengineered tools microscale control, long‐term culture, real‐time data acquisition from such aid elucidating underlying mechanisms. Here, a new culturing, manipulation analysis platform is described to study thin polycarbonate film‐based microdevices. A pipeline consisting open‐source software quantify 3D movement 4D image analyze migration within multicellular clusters. It shown that be used non‐adherent cultures P19C5 mouse stem line cells (mESCs) show symmetry breaking axial elongation events similar early development. Using platform, it found localized proliferation coordinated result aggregates. Further, polarization mESC aggregates dependent directed migration.

Language: Английский

Citations

19
Collective behaviours in organoids DOI Creative Commons
Qiutan Yang, Prisca Liberali

Current Opinion in Cell Biology, Journal Year: 2021, Volume and Issue: 72, P. 81 - 90

Published: July 28, 2021

Collective behaviour emerges from interacting units within communities, such as migrating herds, swimming fish schools, and cells tissues. At the microscopic level, collective behaviours include cell migration in development cancer invasion, rhythmic gene expression pattern formation, competition homeostasis cancer, force generation mechano-sensing morphogenesis. Studying initiation maintenance of is key to understand principles development, regeneration disease. However, manifold influences contributing factors vivo environments challenge dissection causalities animal models. As an alternative model that has emerged overcome this difficulty, vitro three-dimensional organoid cultures provide a reductionist approach yet retain similarities with tissue cellular composition organisation. Here, we focus on recent progresses studying different systems discuss their advantages possibility improvement for future applications.

Language: Английский

Citations

18