Current Opinion in Cell Biology,
Journal Year:
2021,
Volume and Issue:
72, P. 81 - 90
Published: July 28, 2021
Collective
behaviour
emerges
from
interacting
units
within
communities,
such
as
migrating
herds,
swimming
fish
schools,
and
cells
tissues.
At
the
microscopic
level,
collective
behaviours
include
cell
migration
in
development
cancer
invasion,
rhythmic
gene
expression
pattern
formation,
competition
homeostasis
cancer,
force
generation
mechano-sensing
morphogenesis.
Studying
initiation
maintenance
of
is
key
to
understand
principles
development,
regeneration
disease.
However,
manifold
influences
contributing
factors
vivo
environments
challenge
dissection
causalities
animal
models.
As
an
alternative
model
that
has
emerged
overcome
this
difficulty,
vitro
three-dimensional
organoid
cultures
provide
a
reductionist
approach
yet
retain
similarities
with
tissue
cellular
composition
organisation.
Here,
we
focus
on
recent
progresses
studying
different
systems
discuss
their
advantages
possibility
improvement
for
future
applications.
Tissue Engineering Part A,
Journal Year:
2019,
Volume and Issue:
25(19-20), P. 1341 - 1346
Published: Aug. 14, 2019
Cells
are
inherently
conferred
with
the
ability
to
self-organize
into
tissues
and
organs
comprising
human
body.
Self-organization
can
be
recapitulated
in
vitro
recent
advances
organoid
field
just
one
example
of
how
we
generate
small
functioning
elements
organs.
Tissue
engineers
benefit
from
power
self-organization
should
consider
they
harness
enhance
process
their
constructs.
For
example,
aggregates
stem
cells
tissue-specific
input
carefully
selected
biomolecules
guide
differentiation
toward
a
mature
phenotype.
This
further
enhanced
by
use
technologies
provide
physiological
microenvironment
for
self-organization,
size
constructs,
enable
long-term
culture
self-organized
structures.
Of
importance,
conducting
limited
fine-tuning
avoid
over-engineering
that
could
counteract
inherent
cellular
self-organization.
is
powerful
innate
feature
fine-tuned
but
not
over-engineered
create
new
Advanced Materials,
Journal Year:
2020,
Volume and Issue:
32(24)
Published: April 29, 2020
Abstract
Recently
developed
stem‐cell‐based
in
vitro
models
of
morphogenesis
can
help
shed
light
on
the
mechanisms
involved
embryonic
patterning.
These
are
showcased
using
traditional
cell
culture
platforms
and
materials,
which
allow
limited
control
over
biological
system
usually
do
not
support
high‐content
imaging.
In
contrast,
advanced
microengineered
tools
microscale
control,
long‐term
culture,
real‐time
data
acquisition
from
such
aid
elucidating
underlying
mechanisms.
Here,
a
new
culturing,
manipulation
analysis
platform
is
described
to
study
thin
polycarbonate
film‐based
microdevices.
A
pipeline
consisting
open‐source
software
quantify
3D
movement
4D
image
analyze
migration
within
multicellular
clusters.
It
shown
that
be
used
non‐adherent
cultures
P19C5
mouse
stem
line
cells
(mESCs)
show
symmetry
breaking
axial
elongation
events
similar
early
development.
Using
platform,
it
found
localized
proliferation
coordinated
result
aggregates.
Further,
polarization
mESC
aggregates
dependent
directed
migration.
Current Opinion in Cell Biology,
Journal Year:
2021,
Volume and Issue:
72, P. 81 - 90
Published: July 28, 2021
Collective
behaviour
emerges
from
interacting
units
within
communities,
such
as
migrating
herds,
swimming
fish
schools,
and
cells
tissues.
At
the
microscopic
level,
collective
behaviours
include
cell
migration
in
development
cancer
invasion,
rhythmic
gene
expression
pattern
formation,
competition
homeostasis
cancer,
force
generation
mechano-sensing
morphogenesis.
Studying
initiation
maintenance
of
is
key
to
understand
principles
development,
regeneration
disease.
However,
manifold
influences
contributing
factors
vivo
environments
challenge
dissection
causalities
animal
models.
As
an
alternative
model
that
has
emerged
overcome
this
difficulty,
vitro
three-dimensional
organoid
cultures
provide
a
reductionist
approach
yet
retain
similarities
with
tissue
cellular
composition
organisation.
Here,
we
focus
on
recent
progresses
studying
different
systems
discuss
their
advantages
possibility
improvement
for
future
applications.