Two‐Photon Polymerization Lithography for Optics and Photonics: Fundamentals, Materials, Technologies, and Applications

Advanced Functional Materials, Год журнала: 2023, Номер 33(39)

Опубликована: Март 22, 2023

Abstract The rapid development of additive manufacturing has fueled a revolution in various research fields and industrial applications. Among the myriad advanced 3D printing techniques, two‐photon polymerization lithography (TPL) uniquely offers significant advantage nanoscale print resolution, been widely employed diverse fields, for example, life sciences, materials mechanics, microfluidics. More recently, by virtue optical transparency most resins used, TPL is finding new applications optics photonics, with nanometer to millimeter feature dimensions. It enables minimization elements systems, exploration light‐matter interactions degrees freedom, never possible before. To review recent progress related research, it starts fundamentals material formulation, then discusses novel fabrication methods, wide range These notably include diffractive, topological, quantum, color optics. With panoramic view development, concluded insights perspectives future potential

Язык: Английский

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High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues

Science Advances, Год журнала: 2023, Номер 9(8)

Опубликована: Фев. 22, 2023

Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying requirements of high cell density (HCD), viability, and fine fabrication resolution. In particular, resolution digital light processing-based 3D suffers with increasing bioink due scattering. We developed a novel approach mitigate this scattering-induced deterioration The inclusion iodixanol enables 10-fold reduction scattering substantial improvement for bioinks an HCD. Fifty-micrometer was achieved 0.1 billion per milliliter density. To showcase potential application tissue/organ bioprinting, HCD thick tissues vascular networks were fabricated. viable perfusion culture system, endothelialization angiogenesis observed after 14 days culture.

Язык: Английский

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Controlling Light in Scattering Materials for Volumetric Additive Manufacturing

Advanced Science, Год журнала: 2022, Номер 9(22)

Опубликована: Май 18, 2022

3D printing has revolutionized the manufacturing of volumetric components and structures in many areas. Several fully light-based techniques have been recently developed thanks to advent photocurable resins, promising reach unprecedented short print time (down a few tens seconds) while keeping good resolution (around 100 μm). However, these new approaches only work with homogeneous relatively transparent resins so that light patterns used for photo-polymerization are not scrambled along their propagation. Herein, method takes into account scattering resin prior computing projection is proposed. Using tomographic printer, it experimentally demonstrated implementation this correction critical when objects whose size exceeds mean free path. To show broad applicability technique, functional high fidelity fabricated hard organic acrylates soft cell-laden hydrogels (at 4 million cells mL-1 ). This opens up perspectives inside turbid materials particular interesting applications bioprinting constructs.

Язык: Английский

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Breathable, antifreezing, mechanically skin-like hydrogel textile wound dressings with dual antibacterial mechanisms

Bioactive Materials, Год журнала: 2022, Номер 21, С. 313 - 323

Опубликована: Сен. 13, 2022

Hydrogels are emerging as the most promising dressings due to their excellent biocompatibility, extracellular matrix mimicking structure, and drug loading ability. However, existing hydrogel exhibit limited breathability, poor environmental adaptability, potential resistance, options, which extremely restrict therapeutic effect working scenarios. Here, current research introduces first paradigm of textile based on novel gelatin glycerin (glyhydrogel) fibers fabricated by Hofmeister wet spinning. Benefiting from unique knitted dressing features breathability (1800 times that commercially available 3 M dressing) stretchability (535.51 ± 38.66%). Furthermore, glyhydrogel can also withstand extreme temperature -80 °C, showing for application in subzero environments. Moreover, introduction endows with remarkable antibacterial property expands selection loaded drugs (e.g., clindamycin). The prepared shows an infected wound healing a complete rat skin closure within 14 days. All these functions have not been achievable traditional provide new approach development dressings.

Язык: Английский

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Light-based vat-polymerization bioprinting

Nature Reviews Methods Primers, Год журнала: 2023, Номер 3(1)

Опубликована: Июнь 22, 2023

Язык: Английский

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Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges

Trends in biotechnology, Год журнала: 2022, Номер 41(5), С. 604 - 614

Опубликована: Дек. 10, 2022

Bioprinting aims to produce 3D structures from which embedded cells can receive mechanical and chemical stimuli that influence their behavior, direct organization migration, promote differentiation, in a similar way what happens within the native extracellular matrix. However, limited spatial resolution has been bottleneck for conventional bioprinting approaches. Reproducing fine features at cellular scale, while maintaining reasonable printing volume, is necessary enable biofabrication of more complex functional tissue organ models. In this opinion article we recount emergence of, discuss most promising, high-definition (HD) techniques achieve goal, discussing obstacles remain be overcome, applications are envisioned engineering field.

Язык: Английский

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Progress of Microfluidic Hydrogel‐Based Scaffolds and Organ‐on‐Chips for the Cartilage Tissue Engineering

Advanced Materials, Год журнала: 2023, Номер 35(26)

Опубликована: Янв. 12, 2023

Abstract Cartilage degeneration is among the fundamental reasons behind disability and pain across globe. Numerous approaches have been employed to treat cartilage diseases. Nevertheless, none shown acceptable outcomes in long run. In this regard, convergence of tissue engineering microfabrication principles can allow developing more advanced microfluidic technologies, thus offering attractive alternatives current treatments traditional constructs used applications. Herein, developments involving hydrogel‐based scaffolds, promising structures for regeneration, ranging from hydrogels with channels prepared by devices, that enable therapeutic delivery cells, drugs, growth factors, as well cartilage‐related organ‐on‐chips are reviewed. Thereafter, anatomy types damages, present treatment options briefly overviewed. Various introduced, advantages scaffolds over thoroughly discussed. Furthermore, available technologies fabricating chips presented. The preclinical clinical applications regeneration development time further explained. developments, recent key challenges, prospects should be considered so develop systems repair highlighted.

Язык: Английский

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Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics

Advanced Materials, Год журнала: 2023, Номер 35(32)

Опубликована: Апрель 26, 2023

Major challenges in biofabrication revolve around capturing the complex, hierarchical composition of native tissues. However, individual 3D printing techniques have limited capacity to produce composite biomaterials with multi-scale resolution. Volumetric bioprinting recently emerged as a paradigm-shift biofabrication. This ultrafast, light-based technique sculpts cell-laden hydrogel bioresins into structures layerless fashion, providing enhanced design freedom over conventional bioprinting. it yields prints low mechanical stability, since soft, cell-friendly hydrogels are used. Herein, possibility converge volumetric melt electrowriting, which excels at patterning microfibers, is shown for fabrication tubular hydrogel-based composites behavior. Despite including non-transparent electrowritten scaffolds process, high-resolution bioprinted successfully achieved. Tensile, burst, and bending properties printed tubes tuned altering mesh design, resulting multi-material constructs customizable, anisotropic geometries that better mimic intricate biological structures. As proof-of-concept, engineered obtained by building trilayered vessels, features (valves, branches, fenestrations) can be rapidly using this hybrid approach. multi-technology convergence offers new toolbox manufacturing mechanically tunable living

Язык: Английский

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Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(17)

Опубликована: Фев. 19, 2023

Gelatin is a widely utilized bioprinting biomaterial due to its cell-adhesive and enzymatically cleavable properties, which improve cell adhesion growth. often covalently cross-linked stabilize bioprinted structures, yet the matrix unable recapitulate dynamic microenvironment of natural extracellular (ECM), thereby limiting functions cells. To some extent, double network bioink can provide more ECM-mimetic, niche for More recently, gelatin matrices are being designed using reversible cross-linking methods that emulate mechanical properties ECM. This review analyzes progress in developing formulations 3D culture, critically techniques, with focus on strategies optimize discusses new chemistries viscoelastic, stress-relaxing ECM, enable advanced functions, less explored engineering bioink. Finally, this work presents perspective areas future research argues next generation bioinks should be by considering cell-matrix interactions, constructs validated against currently established culture standards achieve improved therapeutic outcomes.

Язык: Английский

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Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion‐Volumetric Printing of Microgels

Advanced Materials, Год журнала: 2023, Номер 35(36)

Опубликована: Июнь 3, 2023

In living tissues, cells express their functions following complex signals from surrounding microenvironment. Capturing both hierarchical architectures at the micro- and macroscale, anisotropic cell patterning remains a major challenge in bioprinting, bottleneck toward creating physiologically-relevant models. Addressing this limitation, novel technique is introduced, termed Embedded Extrusion-Volumetric Printing (EmVP), converging extrusion-bioprinting layer-less, ultra-fast volumetric allowing spatially pattern multiple inks/cell types. Light-responsive microgels are developed for first time as bioresins (µResins) light-based providing microporous environment permissive homing self-organization. Tuning mechanical optical properties of gelatin-based microparticles enables use support bath suspended extrusion printing, which features containing high densities can be easily introduced. µResins sculpted within seconds with tomographic light projections into centimeter-scale, granular hydrogel-based, convoluted constructs. Interstitial microvoids enhanced differentiation stem/progenitor (vascular, mesenchymal, neural), otherwise not possible conventional bulk hydrogels. As proof-of-concept, EmVP applied to create synthetic biology-inspired intercellular communication models, where adipocyte regulated by optogenetic-engineered pancreatic cells. Overall, offers new avenues producing regenerative grafts biological functionality, developing engineered systems (metabolic) disease

Язык: Английский

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