Inhalable liposomal delivery of osimertinib and DNA for treating primary and metastasis lung cancer

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 8, 2025

Lung cancer remains one of the most common malignancies, and its brain metastases significantly worsen prognosis for patients. Current treatments lung face many challenges, including poor drug accumulation inability to simultaneously control primary metastatic tumors. Here, we show that mRNA-binding protein insulin-like growth factor 3 is crucial non-small cell progression metastasis. We construct an inhalable nanoliposome system co-deliver osimertinib DNA plasmid gene knockdown. Upon inhalation, these nanoparticles efficiently penetrate pulmonary barriers accumulate in lungs by mimicking natural surfactants. Within tumor cells, released inhibits growth, while triggers production engineered exosomes can travel suppress This strategy effectively both tumors enhancing antitumor immune responses. work suggests this nanomedicine offers a safe versatile therapy.

Язык: Английский

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Immunoregulation and male reproductive function: Impacts and mechanistic insights into inflammation

Andrology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Abstract This paper investigates the complex relationship between immune system and male reproductive processes, emphasizing how chronic inflammation can adversely affect health. The plays a dual role; it protects regulates organs spermatogenesis while maintaining health through privilege in testes activities of various cells cytokines. However, when persists or intensifies, disrupt this balance, leading to attacks on tissues resulting infertility.This study provides detailed analysis impair sperm production, quality, secretion gonadal hormones both directly indirectly. It also delves into critical roles testicular privilege, cells, cytokines sustaining examines impacts infections, autoimmune diseases, environmental factors fertility.

Язык: Английский

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ICAM-1-decorated extracellular vesicles loaded with miR-146a and Glut1 drive immunomodulation and hinder tumor progression in a murine model of breast cancer

Biomaterials Science, Год журнала: 2023, Номер 11(20), С. 6834 - 6847

Опубликована: Янв. 1, 2023

Tumor-associated immune cells play a crucial role in cancer progression. Myeloid-derived suppressor (MDSCs), for example, are immature innate that infiltrate the tumor to exert immunosuppressive activity and protect from host's system and/or cancer-specific immunotherapies. While tumor-associated have emerged as promising therapeutic target, efforts counter immunosuppression within niche been hampered by lack of approaches selectively target cell compartment tumor, effectively eliminate "tumor-protecting" drive an "anti-tumor" phenotype. Here we report on novel nanotechnology-based approach promote responses murine model breast cancer. Engineered extracellular vesicles (EVs) decorated with ICAM-1 ligands loaded miR-146a Glut1, were biosynthesized (in vitro or vivo) administered tumor-bearing mice once week up 5 weeks. The impact this treatment modality progression was evaluated via RT-qPCR, flow cytometry, histology. Our results indicate weekly administration engineered EVs (i.e., ICAM-1-decorated Glut1) compared no cargo. Flow cytometry analyses tumors indicated shift phenotype population toward more pro-inflammatory state, which appeared facilitated infiltration tumor-targeting T cells, associated reduction size decreased metastatic burden. Altogether, our could be powerful platform nanotechnology deployment cell-targeting therapies solid tumors.

Язык: Английский

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Natural Killer Cell‐Derived Extracellular Vesicles as Potential Anti‐Viral Nanomaterials

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(19)

Опубликована: Апрель 27, 2024

Abstract In viral infections, natural killer (NK) cells exhibit anti‐viral activity by inducing apoptosis in infected host and impeding replication through heightened cytokine release. Extracellular vesicles derived from NK (NK‐EVs) also contain the membrane composition, homing capabilities, cargo that enable activity. These characteristics, their biocompatibility low immunogenicity, give NK‐EVs potential to be a viable therapeutic platform. This study characterizes size, EV‐specific protein expression, cell internalization, biocompatibility, miRNA evaluate properties of NK‐EVs. After 48 h NK‐EV incubation inflamed A549 lung epithelial cells, or conditions mimic infections such as during COVID‐19, treated with upregulated (miR‐27a, miR‐27b, miR‐369‐3p, miR‐491‐5p) compared non‐treated controls control EVs cells. Additionally, effectively reduce expression RNA pro‐inflammatory (TNF‐α, IL‐8) levels SARS‐CoV‐2 Vero E6 kidney mice without causing tissue damage while significantly decreasing controls. Herein, this work elucidates safe, nanomaterials, offering promising alternative conventional therapies.

Язык: Английский

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Potential of Exosomes as Multifunctional Nanocarriers for Targeted Drug Delivery

Molecular Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 13, 2024

Язык: Английский

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