ACS Catalysis, Год журнала: 2024, Номер 14(21), С. 16392 - 16422
Опубликована: Окт. 23, 2024
Язык: Английский
Advanced Functional Materials, Год журнала: 2024, Номер 34(45)
Опубликована: Июнь 3, 2024
Abstract Multi‐component deoxyribozymes (MNAzymes) have shown extraordinary potential in precise gene therapy vitro, however, the vivo application is limited by complicated delivery systems. Herein, a novel DNA‐metal binding mechanism discovered, and metal‐nucleic acid frameworks (MNFs) are built composed of MNAzymes metal ions, which enable carrier‐free self‐delivery MNAzymes. Metal ions high affinity to DNA, metals with DNA at 20–30 base pair long (that normally MNAzyme has) form MNF structure challenged stringent high‐temperature synthesis conditions, poor stability products, lack targeting capabilities. While, it discovered that through folding entanglement an aptamer tail, prolonging sequence 71 pair, significantly improved stabilized even room temperature. Moreover, tail also endows MNFs As proof concept, Ca/MNAzyme system temperature, loaded model imaging protein BSA‐Cy5 synthesized. This can effectively target Her‐2 positive gastric cancer cells responsive initiate dual regulation, thereby inducing energy depletion cells.
Язык: Английский
Процитировано
4
Frontiers in Molecular Biosciences, Год журнала: 2023, Номер 10
Опубликована: Сен. 11, 2023
Based on the development of nucleic acid therapeutic drugs, DNAzymes obtained through in vitro selection technology 1994 are gradually being sought. single-stranded DNA molecules with catalytic function, which specifically cleave RNA under action metal ions. Various vivo and models have recently demonstrated that can target related genes cancer, cardiovascular disease, bacterial viral infection, central nervous system disease. Compared other therapy gained more attention due to their excellent cutting efficiency, high stability, low cost. Here, We first briefly reviewed characteristics DNAzymes, then discussed disease-targeting inhibition model hoping provide new insights ways for disease treatment. Finally, were still subject some restrictions practical applications, including cell uptake nuclease degradation interference from biological matrices. latest delivery strategy among lipid nanoparticles received widespread successful COVID-19 mRNA vaccine, provides possibility subsequent clinical application DNAzymes. In addition, future was prospected.
Язык: Английский
Процитировано
9
Drug Delivery and Translational Research, Год журнала: 2024, Номер 14(10), С. 2712 - 2726
Опубликована: Фев. 12, 2024
Язык: Английский
Процитировано
3
Aggregate, Год журнала: 2024, Номер 5(4)
Опубликована: Март 31, 2024
Abstract Microfibers from natural products are endowed with remarkable biocompatibility, biodegradability, sustainable utilization as well environmental protection characteristics etc. Benefitting these advantages, microfibers have demonstrated their prominent values in biomedical applications. This review comprehensively summarizes the relevant research progress of and To begin, common elements introduced for microfiber fabrication. After that, focus is on specific fabrication technology process. Subsequently, applications discussed detail. Last but not least, main challenges during development process summarized, followed by a vision future opportunities.
Язык: Английский
Процитировано
3
ACS Catalysis, Год журнала: 2024, Номер 14(21), С. 16392 - 16422
Опубликована: Окт. 23, 2024
Язык: Английский
Процитировано
3