DNAzyme hydrogels specifically inhibit the NLRP3 pathway to prevent radiation-induced skin injury in mice DOI Creative Commons
Daijun Zhou, Zhihui Li, Bao Li

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 22, 2025

Radiation-induced skin injury (RISI) is a frequent complication of radiotherapy, yet current preventive strategies exhibit suboptimal efficacy. Our previous publications have consistently demonstrated the effectiveness biomaterials and hydrogels in preventing RISI. Based on comprehensive literature reviews, we speculate that NLRP3 overexpression plays central role development Therefore, designing DNAzyme (DZ)-hydrogels with targeted inhibition crucial for RISI.To achieve this, designed screened optimal NLRP3-DZ using bioinformatics, molecular dynamics, gel electrophoresis methods. We encapsulated within ZIF-8 to enhance its stability, controlled release, safety. To material's transdermal penetration practicality, attached TAT transmembrane peptide. The final preparation characterization NLRP3-DZ@ZIF-8/TAT was achieved.In vitro cell models revealed DZ-hydrogels high biosafety, effectively inhibit expression, promote migration, apoptosis, possess antibacterial properties. Genomics analysis suggested may exert these functions by regulating changes relevant mRNA pathways.Furthermore, established mouse model RISI found material can wound healing proteins associated oxidative stress, inflammatory response. These research findings provide valuable insights prevention DZ-hydrogels.

Language: Английский

Host-Guest Recognition-Mediated Reversible and Orthogonal Regulation of DNAzyme Activity DOI
Xiao Lin,

Zhu Haitao,

Yanmei Gao

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The host–guest recognition system is incorporated into the core region of 10–23 DNAzyme for precise regulation its cleavage functionality in test tubes and living cells.

Language: Английский

Citations

0
A Trinity DNAzyme Circuit with Dual‐Confinement Effect for Imaging‐Guided Combined Gene/Chemo‐Dynamic Therapy DOI Open Access
Shuang G. Zhao,

Xuesong Wang,

Ruijia Deng

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Synthetic catalytic DNAzyme circuits have gained great attention as versatile theranostic toolboxes, but their accuracy and efficiency is restricted by uncontrolled signal leakage inefficient circuitry activation. Here, a trinity circuit (TriDC) constructed using unique logic gate bioswitchable DNA tetrahedral walker@MnO 2 nanosheets structure. The AND walker, composed of dual allosteric (termed “commander” “soldier”) scaffold, used to execute imaging‐guided gene therapy avoiding undesired cascade “walking” in confined space. Glutathione‐mediated reduction MnO provides secondary confinement stack the walker situ, offering abundant Mn 2+ cofactor achieve self‐sufficiency induce chemo‐dynamic therapy. structure increases local reaction concentrations ≈100‐fold according collision frequency model, enabling detection microRNA‐10b microRNA‐155 at limit 98.97 p m , thereby facilitating precise imaging breast cancer cells. combined gene/chemo‐dynamic achieves 66.2% tumor growth inhibition efficiency. TriDC offers novel insights into theranostics also presents paradigm for situ implementation higher‐order circuits.

Language: Английский

Citations

0
Current landscape of metal–organic framework-mediated nucleic acid delivery and therapeutics DOI

Shi‐wei Feng,

Yan Li, Zheng Tan

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125295 - 125295

Published: Feb. 1, 2025

Language: Английский

Citations

0
Recent advances in DNA nanotechnology for cancer detection and therapy: A review DOI

Donya Esmaeilpour,

Matineh Ghomi, Ehsan Nazarzadeh Zare‬

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142136 - 142136

Published: March 1, 2025

Language: Английский

Citations

0
DNAzyme hydrogels specifically inhibit the NLRP3 pathway to prevent radiation-induced skin injury in mice DOI Creative Commons
Daijun Zhou, Zhihui Li, Bao Li

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 22, 2025

Radiation-induced skin injury (RISI) is a frequent complication of radiotherapy, yet current preventive strategies exhibit suboptimal efficacy. Our previous publications have consistently demonstrated the effectiveness biomaterials and hydrogels in preventing RISI. Based on comprehensive literature reviews, we speculate that NLRP3 overexpression plays central role development Therefore, designing DNAzyme (DZ)-hydrogels with targeted inhibition crucial for RISI.To achieve this, designed screened optimal NLRP3-DZ using bioinformatics, molecular dynamics, gel electrophoresis methods. We encapsulated within ZIF-8 to enhance its stability, controlled release, safety. To material's transdermal penetration practicality, attached TAT transmembrane peptide. The final preparation characterization NLRP3-DZ@ZIF-8/TAT was achieved.In vitro cell models revealed DZ-hydrogels high biosafety, effectively inhibit expression, promote migration, apoptosis, possess antibacterial properties. Genomics analysis suggested may exert these functions by regulating changes relevant mRNA pathways.Furthermore, established mouse model RISI found material can wound healing proteins associated oxidative stress, inflammatory response. These research findings provide valuable insights prevention DZ-hydrogels.

Language: Английский

Citations

0