Gold nanoclusters encapsulated microneedle patches with antibacterial and self‐monitoring capacities for wound management

Aggregate, Год журнала: 2024, Номер 5(3)

Опубликована: Янв. 25, 2024

Abstract The management of infected wounds is always great significance and urgency in clinical biomedical fields. Recent efforts this area are focusing on the development functional wound patches with effective antibacterial, drug delivery, sensor properties. Here, we present novel hyaluronic acid (HA) microneedle these features by encapsulating aminobenzeneboronic acid‐modified gold nanoclusters (A‐GNCs) for management. A‐GNCs loaded were derived from negative‐mold replication showed high mechanical strength to penetrate skin. release was realized degradation HA, self‐monitor released actives based dynamic bright orange fluorescence emitted under ultraviolet radiation. As could destroy bacteria membranes, excellent vitro antibiosis ability. Based features, have demonstrated inhibition, residual self‐monitoring, healing promotion abilities Escherichia coli‐ or Staphylococcus aureus ‐infected These results indicated potential such applications.

Язык: Английский

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Fe-Capsaicin Nanozymes Attenuate Sepsis-Induced Acute Lung Injury via NF-κB Signaling

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 73 - 90

Опубликована: Янв. 1, 2024

Background: In sepsis, the lungs are one of most severely affected organs, usually resulting in acute lung injury (ALI). Capsaicin (CAP) is a natural compound found chili peppers that has pain-relieving and anti-inflammatory properties. Here, we report nanoparticles containing capsaicin iron (Fe-CAP NPs) exhibited effects treatment ALI. Methods: The morphological characteristics nanozymes were detected. RAW 264.7 cells divided into four groups: control, lipopolysaccharide (LPS), CAP+LPS Fe-CAP+LPS groups. expression inducible nitric oxide synthase (iNOS), transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α) was assessed by immunofluorescence, Western blot, enzyme-linked immunosorbent assay (ELISA). Nuclear factor kappa-B (NF-κB) determined blot. C57 mice LPS, Interleukin-6 (IL-6) iNOS detected Blot. IL-6 TNF-α serum ELISA. Extravasated Evans blue, histopathological evaluation wet-to-dry (W/D) weight ratio used to assess pulmonary capillary permeability. blood major organs (heart, liver, spleen, kidney) tested for toxicity Fe-CAP NPs. Results: LPS group, TNF-α, iNOS, p-NF-κB p-IKBα increased. However, their significantly decreased group. TGF-β showed opposite trend. vivo, notably increased group but with pretreatment. ameliorated EB leakage, improved histopathology tissue reduced W/D ratio. non-cytotoxicity, when studying these biological activities. Conclusion: NPs could alleviated inflammation inhibiting pro-inflammatory factors macrophages, increasing factors, alleviating damage. Keywords: capsaicin, macrophage, nanoparticles,

Язык: Английский

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Nanotechnology‐driven strategies to enhance the treatment of drug‐resistant bacterial infections

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2024, Номер 16(3)

Опубликована: Май 1, 2024

Abstract The misuse of antibiotics has led to increased bacterial resistance, posing a global public health crisis and seriously endangering lives. Currently, antibiotic therapy remains the most common approach for treating infections, but its effectiveness against multidrug‐resistant bacteria is diminishing due slow development new increase drug resistance. Consequently, developing a\ntimicrobial strategies improving efficacy combat infection become an urgent priority. emergence nanotechnology revolutionized traditional treatment, presenting opportunities refractory infection. Here we comprehensively review research progress in nanotechnology‐based antimicrobial delivery highlight diverse platforms designed target different resistance mechanisms. We also outline use combining with other therapeutic modalities enhance drug‐resistant infections. These innovative have potential susceptibility overcome Finally, challenges prospects application nanomaterial‐based combating are discussed. This article categorized under: Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures Therapeutic Approaches Drug Discovery Nanomedicine Infectious Disease

Язык: Английский

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A self‐regulated phototheranostic nanosystem with single wavelength‐triggered energy switching and oxygen supply for multimodal synergistic therapy of bacterial biofilm infections

Aggregate, Год журнала: 2024, Номер 5(5)

Опубликована: Май 13, 2024

Abstract The exploration of antibiotic‐independent phototherapy strategies for the treatment bacterial biofilm infections has gained significant attention. However, efficient eradication biofilms remains a challenge. Herein, self‐regulated phototheranostic nanosystem with single wavelength‐triggered photothermal therapy (PTT)/photodynamic (PDT) transformation and oxygen supply multimodal synergistic is presented. This approach combines eutectic mixture natural phase‐change materials (PCMs) an aggregation‐induced emission (AIE) agent TPA‐ICN to form colloidally stable nanopartcicles (i.e. AIE@PCM NPs). reversible solid−liquid phase transition PCMs facilitates adaptive regulation aggregation states TPA‐ICN, enabling switch between energy dissipation pathways enhanced PDT in solid or PTT liquid PCMs. Additionally, oxygen‐carrying thermoresponsive nanoparticles are also introduced alleviate hypoxic microenvironment by releasing upon heating NPs PTT. exhibits outstanding therapeutic efficacy against both vitro vivo, antibacterial efficiency 99.99%. study utilizes theranostic nanoplatform PTT/PDT via heat‐triggered release, holding great promise safe infections.

Язык: Английский

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Multifunctional nanozymes for disease diagnosis and therapy

Biomedical Journal, Год журнала: 2024, Номер 47(4), С. 100699 - 100699

Опубликована: Янв. 24, 2024

The development of nanotechnology has brought about groundbreaking advancements in diseases' diagnostics and therapeutics. Among them, multifunctional nanomaterials with enzyme-like activities (i.e., nanozymes) featured high stability, large surface area for bioconjugation, easy storage, offer unprecedented opportunities disease treatment. Recent years have witnessed the great progress nanozyme-based theranostics. To highlight these achievements, this review first introduces recent on nanozymes biosensing diagnostics. Then, it summarizes applications therapeutics including anti-tumor antibacterial treatment, anti-inflammatory other diseases In addition, several targeted strategies to improve therapeutic efficacy nanozyme are discussed. Finally, challenges field diagnosis therapy summarized.

Язык: Английский

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Versatile Platinum Nanoparticles-Decorated Phage Nanozyme Integrating Recognition, Bacteriolysis, and Catalysis Capabilities for On-Site Detection of Foodborne Pathogenic Strains Vitality Based on Bioluminescence/Pressure Dual-Mode Bioassay

Analytical Chemistry, Год журнала: 2024, Номер 96(21), С. 8782 - 8790

Опубликована: Май 10, 2024

Sensitive and on-site discrimination of live dead foodborne pathogenic strains remains a significant challenge due to the lack appropriate assay signal probes. In this work, versatile platinum nanoparticle-decorated phage nanozyme (P2@PtNPs) that integrated recognition, bacteriolysis, catalysis was designed establish bioluminescence/pressure dual-mode bioassay for determination vitality strains. Benefiting from bacterial strain-level specificity phage, target Salmonella typhimurium (S.T) specially captured form sandwich complexes with P2@PtNPs on another phage-modified glass microbead (GM@P1). As other part nanozyme, introduced PtNPs could not only catalyze decomposition hydrogen peroxide generate oxygen pressure but also produce hydroxyl radicals around bacteria enhance bacteriolysis adenosine triphosphate release. It significantly improved bioluminescence signal. The two signals corresponded total counts, so be easily calculated difference between counts. Meanwhile, S.T realized according ratio S.T. Under optimal conditions, application range proposed 102–107 CFU/mL, limit detections 30 CFU/mL 40 respectively. This work provides an innovative probe food safety.

Язык: Английский

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Surface self-assembly via one-pot polymerization to construct high-strength and antibacterial polyethylene fabric

Chemical Engineering Journal, Год журнала: 2024, Номер 492, С. 152246 - 152246

Опубликована: Май 15, 2024

Язык: Английский

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A Fused Membrane‐Camouflaged Biomimetic Nanosystem for Dual‐Targeted Therapy of Septic Arthritis

Small, Год журнала: 2025, Номер 21(9)

Опубликована: Янв. 19, 2025

Abstract Due to the inherent aseptic and enclosed characteristics of joint cavity, septic arthritis (SA) almost inevitably leads intractable infections rapidly progressing complex pathological environments. Presently, SA faces not only deficient effectiveness gold‐standard systemic antibiotic therapy but also scarcity effective localized targeted approaches standardized animal models. Herein, an ingenious multifunctional nanosystem is designed, which involves methylation hyaluronic acid (HA), copolymerization with DEGDA, loading vancomycin (VAN), then coating fused macrophage‐platelet membrane (denoted as FM@HA@VAN). Upon intra‐articular administration, FM@HA@VAN nanoparticles exhibit sustained retention selectively targeting infected sites, leveraging macrophage‐mediated inflammation homing platelet‐directed bacteria targeting. The acidic microenvironment triggers responsive release vancomycin, leading potent bactericidal effects. Subsequently, exposed HA@VAN are efficiently internalized by activated macrophages, releasing HA alleviate oxidative stress achieve chondroprotection inhibiting pro‐inflammatory cytokines, neutralizing ROS upregulating macrophage M2 polarization. In vivo model experiments confirm efficacy this dual‐targeting antibacterial approach, demonstrating its precision in eradicating bacterial alleviating associated processes, including synovial hyperplasia cartilage erosion. therapeutic nanosystem, coordinated fused‐membranes, holds promise for enhancing treatment SA.

Язык: Английский

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Nanomedicines as disruptors or inhibitors of biofilms: Opportunities in addressing antimicrobial resistance

Journal of Controlled Release, Год журнала: 2025, Номер 381, С. 113589 - 113589

Опубликована: Март 1, 2025

The problem of antimicrobial resistance (AMR) has caused global concern due to its great threat human health. Evidences are emerging for a critical role biofilms, one the natural protective mechanisms developed by bacteria during growth, in resisting commonly used clinical antibiotics. Advances nanomedicines with tunable physicochemical properties and unique anti-biofilm provide opportunities solving AMR risks more effectively. In this review, we summarize five "A" stages (adhesion, amplification, alienation, aging allocation) biofilm formation through which they protect internal bacteria. Aimed at characteristics emphasize design "THAT" principles (targeting, hacking, adhering transport) their interactions biofilms Furthermore, recent progresses multimodal antibacterial nanomedicines, including disruption bactericidal activity, types currently available antibiofilm contained organic inorganic outlined highlighted potential applications development preclinical research. Last but not least, offer perspective effectiveness designed address challenges associated translation.

Язык: Английский

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Mucus-penetrating nanomotor system strengthens mucosal immune response to in situ bacterial vaccine against severe bacterial pneumonia

Biomaterials, Год журнала: 2025, Номер 320, С. 123236 - 123236

Опубликована: Март 3, 2025

Язык: Английский

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