In-situ-sprayed therapeutic hydrogel for oxygen-actuated Janus regulation of postsurgical tumor recurrence/metastasis and wound healing

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 27, 2024

Surgery is the mainstay of treatment modality for malignant melanoma. However, deteriorative hypoxic microenvironment after surgery recognized as a stemming cause tumor recurrence/metastasis and delayed wound healing. Here we design construct sprayable therapeutic hydrogel (HIL@Z/P/H) encapsulating tumor-targeted nanodrug photosynthetic cyanobacteria (PCC 7942) to prevent while promote In postsurgical B16F10 melanoma model in female mice, can disrupt cellular redox homeostasis via photodynamic therapy-induced cascade reactions within cells. Besides, photosynthetically generated O

Язык: Английский

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Mitochondrial Localized In Situ Self‐Assembly Reprogramming Tumor Immune and Metabolic Microenvironment for Enhanced Cancer Therapy

Advanced Materials, Год журнала: 2024, Номер 36(15)

Опубликована: Янв. 8, 2024

Abstract The inherent immune and metabolic tumor microenvironment (TME) of most solid tumors adversely affect the antitumor efficacy various treatments, which is an urgent issue to be solved in clinical cancer therapy. In this study, a mitochondrial localized situ self‐assembly system constructed remodel TME by improving immunogenicity disrupting plasticity cells. peptide‐based drug delivery can pre‐assembled into nanomicelles vitro form functional nanofibers on mitochondria through cascade‐responsive process involving reductive release, targeted enrichment, self‐assembly. organelle‐specific self‐assemblyeffectively switches role mitophagy from pro‐survival pro‐death, finally induces intense endoplasmic reticulum stress atypical type II immunogenic cell death. Disintegration ultrastructure also impedes cells, greatly promotes immunosuppresive remodeling immunostimulatory TME. Ultimately, effectively suppresses metastases, converts cold hot with enhanced sensitivity radiotherapy checkpoint blockade This study offers universal strategy for spatiotemporally controlling supramolecular sub‐organelles determine fate enhance

Язык: Английский

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Combination of biodegradable hydrogel and antioxidant bioadhesive for treatment of breast cancer recurrence and radiation skin injury

Bioactive Materials, Год журнала: 2023, Номер 31, С. 408 - 421

Опубликована: Сен. 2, 2023

Postoperative radiotherapy is the standard method for inhibition of breast cancer recurrence and metastasis, whereas radiation resistant ineluctable skin injury are still key problems encountered in prognosis cancer. Herein, we design an internally implantable biodegradable hydrogel extracutaneously applicable antioxidant bioadhesive to concurrently prevent postoperative tumor radioactive after adjuvant radiotherapy. The silk fibroin/perfluorocarbon loading doxorubicin (DOX) formed by consecutive ultrasonication-induced β-sheets-crosslinked amphiphilic fibroin/perfluorocarbon/DOX nanoemulsion, exhibits continuous release oxygen physiological environment improve hypoxia sensitivity radiotherapy, as well simultaneous DOX finally achieve effective anti-cancer effect. A stretchable fabricated copolymerization α-thioctic acid N, N-diacryloyl-l-lysine, gold nanorods gallic loaded into afford gentle photothermal therapy functions. near-infrared light-induced controlled mild can efficiently eliminate excess free radicals generated promote wound healing. Ultimately, vivo animal studies substantiate efficacy our methodology, wherein post-tumor resection administration concomitant application patch effectively inhibit attenuate progression damage.

Язык: Английский

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EISA in Tandem with ICD to Form In Situ Nanofiber Vaccine for Enhanced Tumor Radioimmunotherapy

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(27)

Опубликована: Июнь 10, 2023

Radiotherapy (RT) can produce a vaccine effect and remodel tumor microenvironment (TME) by inducing immunogenic cell death (ICD) inflammation in tumors. However, RT alone is insufficient to elicit systemic antitumor immune response owing limited antigen presentation, immunosuppressive microenvironment, chronic within the tumor. Here, novel strategy reported for generation of situ peptide-based nanovaccines via enzyme-induced self-assembly (EISA) tandem with ICD. As ICD progresses, peptide Fbp-GD FD pY (Fbp-pY), dephosphorylated alkaline phosphatase (ALP) forms fibrous nanostructure around cells, resulting capture encapsulation autologous antigens produced radiation. Utilizing adjuvant controlled-release advantages self-assembling peptides, this nanofiber effectively increases accumulation lymph nodes cross-presentation antigen-presenting cells (APCs). In addition, inhibition cyclooxygenase 2 (COX-2) expression nanofibers promotes repolarization M2-macrophages into M1 reduces number regulatory T (Tregs) myeloid-derived suppressor (MDSCs) required TME remodeling. result, combination significantly enhances therapeutic on 4T1 tumors compared alone, suggesting promising treatment radioimmunotherapy.

Язык: Английский

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Metabolic reprogramming and therapeutic resistance in primary and metastatic breast cancer

Molecular Cancer, Год журнала: 2024, Номер 23(1)

Опубликована: Ноя. 21, 2024

Metabolic alterations, a hallmark of cancer, enable tumor cells to adapt their environment by modulating glucose, lipid, and amino acid metabolism, which fuels rapid growth contributes treatment resistance. In primary breast metabolic shifts such as the Warburg effect enhanced lipid synthesis are closely linked chemotherapy failure. Similarly, metastatic lesions often display distinct profiles that not only sustain but also confer resistance targeted therapies immunotherapies. The review emphasizes two major aspects: mechanisms driving in both how unique environments sites further complicate treatment. By targeting vulnerabilities at stages, new strategies could improve efficacy existing provide better outcomes for cancer patients.

Язык: Английский

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Recent progress in quantitative analysis of self‐assembled peptides

Exploration, Год журнала: 2024, Номер 4(4)

Опубликована: Янв. 23, 2024

Self-assembled peptides have been among the important biomaterials due to its excellent biocompatibility and diverse functions. Over past decades, substantial progress breakthroughs made in designing self-assembled with multifaceted biomedical applications. The techniques for quantitative analysis, including imaging-based techniques, chromatographic technique computational approach (molecular dynamics simulation), are becoming powerful tools exploring structure, properties, applications, even supramolecular assembly processes of peptides. However, a comprehensive review concerning these remains scarce. In this review, recent investigation biostability, cellular uptake, biodistribution, self-assembly behaviors peptide etc., summarized. Specific applications roles highlighted detail. Finally, challenges outlook field concluded. It is believed that will provide technical guidance researchers peptide-based materials pharmaceuticals, facilitate related research newcomers field.

Язык: Английский

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A Mn²⁺‐Assisted Nanofiber‐Hydrogel Adjuvant for Simultaneous Enhancement of Humoral and Cellular Immune Responses

Advanced Functional Materials, Год журнала: 2024, Номер 34(30)

Опубликована: Апрель 9, 2024

Abstract Limited ability to elicit cellular immune responses has restricted the effectiveness of conventional adjuvants in context cancers. Recent advancements innate activation mechanism investigations have paved way for implementation a “bottom‐up” approach development novel adjuvants. Herein, simple hydrogel adjuvant with uniformly organized nanoscale microstructure, termed MnP gel is devised, by employing self‐assembling peptides incorporated manganese ions (Mn 2+ ). exhibits Mn sustained‐release properties vivo and effectively promotes germinal center formation, thereby facilitating generation antibodies at levels comparable aluminum‐based Moreover, transcends scope humoral immunity, demonstrating robustly trigger positioning it as promising candidate cancer prevention treatments. In conclusion, work introduced well‐defined proof‐of‐concept, simplifying vaccine design opening up new avenues “on‐demand” immunomodulation strategies.

Язык: Английский

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PdRu bimetallic nanoalloys with improved photothermal effect for amplified ROS-mediated tumor therapy

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 12

Опубликована: Янв. 3, 2025

An emerging strategy in cancer therapy involves inducing reactive oxygen species (ROS), specifically within tumors using nanozymes. However, existing nanozymes suffer from limitations such as low reactivity, poor biocompatibility, and limited targeting capabilities, hindering their therapeutic efficacy. In response, the PdRu@PEI bimetallic nanoalloys were constructed with well-catalytic activities effective separation of charges, which can catalyze hydrogen peroxide (H2O2) to toxic hydroxyl radical (·OH) under near-infrared laser stimulation. Through facilitating electron transfer enhancing active sites, enhanced peroxidase-like (POD-like) enzymatic activity glutathione (GSH) depletion abilities are boosted through a simple co-reduction process, leading promising anti-tumor activity. The between Pd Ru contributes POD-like Then, by oxidizing endogenous overexpressed GSH, cycling prevents GSH consuming ROS. Furthermore, surface plasmon resonance effect on ensures its photothermal performance local heating, further promoting integrated multi-modal approach has demonstrated significant anti-cancer effects vivo studies. exhibit high catalytic efficiency excellent offering valuable insights for development nano-catalysts/enzymes biomedical applications.

Язык: Английский

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Cancer Stem‐Like Cells‐Oriented Surface Self‐Assembly to Conquer Radioresistance

Advanced Materials, Год журнала: 2023, Номер 35(38)

Опубликована: Июнь 8, 2023

Abstract Cancer stem‐like cells (CSCs), capable of indefinite self‐renewal and differentiation, are considered to be the root cause tumor radiotherapy (RT) resistance. However, CSCs‐targeted therapy still remains a great challenge because they commonly located in deep making drugs hard approach, their hypoxic acidic niche can further aggravate radioresistance. Herein, based on finding that CSCs highly express carbonic anhydrase IX (CAIX) cell membrane, CAIX‐targeted induced situ self‐assembly system surface CSC is reported overcome CSC‐mediated Via sequential processes “monomer release‐target accumulation‐surface self‐assembly”, constructed peptide‐based drug delivery (CA‐Pt) exhibits advantages penetration, amplified CAIX inhibition, enhanced cellular uptake, which greatly relieves microenvironment promote differentiation combines with platinum boost RT‐inducing DNA damage. In both lung cancer mouse zebrafish embryo models, CA‐Pt treatment effectively assist RT suppressing growth preventing invasion metastasis. This study uses surface‐induced strategy differentiate CSCs, may provide universal for overcoming

Язык: Английский

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Metabolic gatekeepers: harnessing tumor-derived metabolites to optimize T cell-based immunotherapy efficacy in the tumor microenvironment

Cell Death and Disease, Год журнала: 2024, Номер 15(10)

Опубликована: Окт. 26, 2024

Язык: Английский

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