bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 22, 2024
Abstract
Current
challenges
in
tissue
engineering
include
creation
of
extracellular
environments
that
support
and
interact
with
cells
using
biochemical,
mechanical,
structural
cues.
Spatial
control
over
these
cues
is
currently
limited
due
to
a
lack
suitable
fabrication
techniques.
This
study
introduces
Xolography,
an
emerging
dual-color
light-sheet
volumetric
printing
technology,
achieve
mechanical
features
for
hydrogel-based
photoresins
at
micro-to
macroscale
while
within
minutes.
We
propose
water-soluble
photoswitch
photoinitiator
system
are
the
first
demonstrate
Xolography
library
naturally-derived,
synthetic,
thermoresponsive
hydrogels.
Centimeter-scale,
three-dimensional
constructs
positive
20
µm
negative
∼
100
fabricated
properties
(compressive
moduli
0.2
kPa
–
6.5
MPa).
Notably,
switching
from
binary
grayscaled
light
projection
enables
spatial
stiffness
(0.2
16
kPa).
As
proof
concept,
leveraged
hydrogels
introduce
reversible
anisotropic
shape
changes
beyond
isometric
shrinkage.
finally
viable
cell
aggregates,
laying
foundation
cell-laden
dynamic,
cell-instructive
tunable
fast
one-step
process.
Overall,
innovations
unlock
unique
possibilities
across
multiple
biomedical
applications.
Chemical Reviews,
Год журнала:
2024,
Номер
124(14), С. 8787 - 8822
Опубликована: Июль 5, 2024
Harnessing
light
for
cross-linking
of
photoresponsive
materials
has
revolutionized
the
field
3D
printing.
A
wide
variety
techniques
leveraging
broad-spectrum
shaping
have
been
introduced
as
a
way
to
achieve
fast
and
high-resolution
printing,
with
applications
ranging
from
simple
prototypes
biomimetic
engineered
tissues
regenerative
medicine.
Conventional
light-based
printing
use
material
in
layer-by-layer
fashion
produce
complex
parts.
Only
recently,
new
emerged
which
deploy
multidirection,
tomographic,
light-sheet
or
filamented
image
projections
deep
into
volume
resin-filled
vat
photoinitiation
cross-linking.
These
Deep
Vat
(DVP)
approaches
alleviate
need
layer-wise
enable
unprecedented
fabrication
speeds
(within
few
seconds)
high
resolution
(>10
μm).
Here,
we
elucidate
physics
chemistry
these
processes,
their
commonalities
differences,
well
emerging
biomedical
non-biomedical
fields.
Importantly,
highlight
limitations,
future
scope
research
that
will
improve
scalability
applicability
DVP
engineering
medicine
applications.
Abstract
Over
the
past
decade,
4D
printing
has
revolutionized
field
of
advanced
manufacturing
by
fabricating
structures
that
dynamically
respond
to
environmental
stimuli.
During
this
process,
shape‐memory
polymers
(SMPs)
stand
out,
enabling
transformations
triggered
temperature,
light,
or
other
factors,
and
show
great
potential
for
applications
in
biomedicine
beyond.
Notably,
biodegradable
SMPs
offer
a
compelling
advantage
medical
devices
due
their
ability
adapt
within
body's
temperature
range
be
absorbed
tissues,
reducing
risks
associated
with
permanent
implants.
While
extrusion
techniques
have
laid
groundwork
biomedicine,
vat
photopolymerization
methods
like
stereolithography
digital
light
processing
are
now
at
forefront,
favored
high
resolution
flexibility
material
design.
However,
search
suitable
materials
these
continues,
current
research
focusing
on
developing
systems
meet
both
mechanical
demands
degradation
profiles
required
applications.
This
review
aims
critically
analyze
advancements
photopolymers,
particularly
elastomers,
discuss
challenges
lie
ahead
clinical
translation.
Advanced Materials Technologies,
Год журнала:
2024,
Номер
9(15)
Опубликована: Апрель 1, 2024
Abstract
Three‐dimensional
(3D)
printed
multimaterials
have
quickly
grown
in
interest
as
researchers
aim
to
mimic
the
material
chemistry
and
geometric
complexity
of
natural
materials.
While
some
exceptional
multimaterial
3D
printing
systems
been
developed,
there
is
still
a
shortfall
scope
production
these
More
recently,
introduction
xolography
single‐step,
auxiliary‐free
additive
manufacturing
approach
has
enabled
rapid
materials
with
geometries
inaccessible
via
other
techniques.
However,
previous
required
resins
high
transparency
for
effective
formation,
formation
not
demonstrated.
In
this
work,
reversible
deactivation
radical
polymerization
used
resin
composition,
viscosity,
processing
parameters
are
explored
develop
new
capable
application
visible
light‐mediated
xolography.
After
optimization,
non‐transparent
containing
thiocarbonylthio
species
applied
manufacture
geometrically
complex,
The
resulting
polymer
network
can
be
functionalized
embedded
moieties
impart
functionality
Finally,
unique
capabilities
demonstrated
featuring
chemically
disparate
domains
3D‐spatial
control.
This
two‐step
overprinting
operationally
simple
provides
avenue
polymeric
printing.
Additive
manufacturing
(AM)
of
ceramics
has
significantly
contributed
to
advancements
in
ceramic
fabrication,
solving
some
the
difficulties
conventional
processing
and
providing
additional
possibilities
for
structure
function
components.
However,
defects
induced
by
layer-by-layer
approach
on
which
traditional
AM
techniques
are
based
still
constitute
a
challenge
address.
This
study
presents
volumetric
SiOC
from
preceramic
polymer
using
xolography,
linear
process
that
allows
avoid
staircase
effect
typical
other
vat
photopolymerization
techniques.
Besides
optimizing
trade-off
between
content
transmittance,
pore
generator
is
introduced
create
transient
channels
gas
release
before
decomposition
organic
constituents
moieties,
resulting
crack-free
solid
structures
even
at
low
yield.
Formulation
optimization
alleviated
sinking
printed
parts
during
printing
prevented
shape
distortion.
Complex
porous
with
smooth
surface
sharp
features
fabricated
under
optimized
parameters.
work
provides
new
method
µm/mm
scale
high
quality
large
geometry
variety
an
efficient
way,
opening
possibility
applications
fields
such
as
micromechanical
systems
microelectronic
Abstract
Digital
light
processing
(DLP)
is
a
projection-based
vat
photopolymerization
3D
printing
technique
that
attracts
increasing
attention
due
to
its
high
resolution
and
accuracy.
The
layer-by-layer
deposition
in
DLP
uses
precise
control
cure
photopolymer
resin
quickly,
providing
smooth
surface
finish
the
uniform
layer
curing
process.
Additionally,
extensive
material
selection
printing,
notably
including
existing
photopolymerizable
materials,
presents
significant
advantage
compared
with
other
techniques
limited
choices.
Studies
can
be
categorized
into
two
main
domains:
material-level
system-level
innovation.
Regarding
innovations,
development
of
photocurable
resins
tailored
rheological,
photocuring,
mechanical,
functional
properties
crucial
for
expanding
application
prospects
technology.
In
this
review,
we
comprehensively
review
state-of-the-art
advancements
focusing
on
innovations
centered
particularly
various
smart
materials
4D
addition
piezoelectric
ceramics
their
composites
applications
DLP.
discuss
recyclable
promote
sustainable
manufacturing
practices.
are
also
delineated,
recent
progress
multi-materials
DLP,
grayscale
AI-assisted
related
developments.
We
highlight
current
challenges
propose
potential
directions
future
development.
Exciting
areas
such
as
creation
stimuli-responsive
functionality,
ceramic
AI-enhanced
still
nascent
stages.
By
exploring
concepts
like
recycling
technology,
integration
these
aspects
unlock
opportunities
driven
by
Through
aim
stimulate
further
interest
encourage
active
collaborations
advancing
systems,
fostering
dynamic
field.
Graphical
abstract
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 2, 2025
Abstract
Offering
high
spatiotemporal
resolution,
dual
wavelength‐controlled
soft
matter
network
formation
paves
the
way
to
advanced
printing
techniques
with
optimized
performance.
One
of
most
promising
approaches
is
antagonistic
control
covalent
bond‐forming
reactions
two
colors
light,
where
photoexcitation
one
wavelength
induces
a
photochemical
reaction,
while
irradiation
other
ceases
it
in
presence
first
color.
Herein,
we
combine
photoactivatable
diene
precursor
and
photoswitchable
dienophile,
establishing
dual‐wavelength‐gated
cycloaddition
reaction
capable
controlling
polymer
crosslinking.
Upon
incorporation
into
methacrylate
copolymer
synthesis
difunctional
dienophile
cross‐linker,
selective
promoted
under
sole
UV
illumination,
can
be
efficiently
suppressed
simultaneous
redlight
irradiation.
Critically,
methodology
used
for
preparation
solid
materials
two‐color
their
cross‐linking
status,
ultimately
allowing
fabrication
spatially
patterned
films.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 27, 2025
Abstract
Current
challenges
in
tissue
engineering
include
creation
of
extracellular
environments
that
support
and
interact
with
cells
using
biochemical,
mechanical,
structural
cues.
Spatial
control
over
these
cues
is
currently
limited
due
to
a
lack
suitable
fabrication
techniques.
This
study
introduces
Xolography,
an
emerging
dual‐color
light‐sheet
volumetric
printing
technology,
achieve
mechanical
features
for
hydrogel‐based
photoresins
at
micro‐
macroscale
while
within
minutes.
A
water‐soluble
photoswitch
photoinitiator
system
library
naturally‐derived,
synthetic,
thermoresponsive
hydrogels
Xolography
are
proposed.
Centimeter‐scale,
3D
constructs
positive
20
µm
negative
≈100
fabricated
properties
(compressive
moduli
0.2
kPa–6.5
MPa).
Notably,
switching
from
binary
grayscaled
light
projection
enables
spatial
stiffness
(0.2–16
kPa).
As
proof
concept,
leveraged
introduce
reversible
anisotropic
shape
changes
beyond
isometric
shrinkage.
viable
cell
aggregates
finally
demonstrated,
laying
the
foundation
cell‐laden
dynamic,
cell‐instructive
tunable
fast
one‐step
process.
Overall,
innovations
unlock
unique
possibilities
across
multiple
biomedical
applications.
