Xolography for Biomedical Applications: Dual-color Light-sheet Printing of Hydrogels with Local Control over Shape and Stiffness DOI Creative Commons

Lena Stoecker,

Gerardo Cedillo-Servin,

Niklas F. König

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract Current challenges in tissue engineering include creation of extracellular environments that support and interact with cells using biochemical, mechanical, structural cues. Spatial control over these cues is currently limited due to a lack suitable fabrication techniques. This study introduces Xolography, an emerging dual-color light-sheet volumetric printing technology, achieve mechanical features for hydrogel-based photoresins at micro-to macroscale while within minutes. We propose water-soluble photoswitch photoinitiator system are the first demonstrate Xolography library naturally-derived, synthetic, thermoresponsive hydrogels. Centimeter-scale, three-dimensional constructs positive 20 µm negative ∼ 100 fabricated properties (compressive moduli 0.2 kPa – 6.5 MPa). Notably, switching from binary grayscaled light projection enables spatial stiffness (0.2 16 kPa). As proof concept, leveraged hydrogels introduce reversible anisotropic shape changes beyond isometric shrinkage. finally viable cell aggregates, laying foundation cell-laden dynamic, cell-instructive tunable fast one-step process. Overall, innovations unlock unique possibilities across multiple biomedical applications.

Language: Английский

Advanced 3D printing accelerates electromagnetic wave absorption from ceramic materials to structures DOI Creative Commons
Wenqing Wang,

Zengchan Li,

Ruyue Su

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 22, 2025

Language: Английский

Citations

2
Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing DOI Creative Commons
Parth Chansoria, Riccardo Rizzo, Dominic Rütsche

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(14), P. 8787 - 8822

Published: July 5, 2024

Harnessing light for cross-linking of photoresponsive materials has revolutionized the field 3D printing. A wide variety techniques leveraging broad-spectrum shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes biomimetic engineered tissues regenerative medicine. Conventional light-based printing use material in layer-by-layer fashion produce complex parts. Only recently, new emerged which deploy multidirection, tomographic, light-sheet or filamented image projections deep into volume resin-filled vat photoinitiation cross-linking. These Deep Vat (DVP) approaches alleviate need layer-wise enable unprecedented fabrication speeds (within few seconds) high resolution (>10 μm). Here, we elucidate physics chemistry these processes, their commonalities differences, well emerging biomedical non-biomedical fields. Importantly, highlight limitations, future scope research that will improve scalability applicability DVP engineering medicine applications.

Language: Английский

Citations

10
Lithography-based 3D printing of hydrogels DOI
Abhishek P. Dhand, Matthew D. Davidson, Jason A. Burdick

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Language: Английский

Citations

8
From pixels to voxels: A mechanistic perspective on volumetric 3D-printing DOI Creative Commons
Quinten Thijssen, Joseph Toombs, Chi Chung Li

et al.

Progress in Polymer Science, Journal Year: 2023, Volume and Issue: 147, P. 101755 - 101755

Published: Oct. 20, 2023

Language: Английский

Citations

18
Advances in 4D printing of biodegradable photopolymers DOI Creative Commons
Yue Ma, Chih‐Jen Shih, Yinyin Bao

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(3)

Published: June 5, 2024

Abstract Over the past decade, 4D printing has revolutionized field of advanced manufacturing by fabricating structures that dynamically respond to environmental stimuli. During this process, shape‐memory polymers (SMPs) stand out, enabling transformations triggered temperature, light, or other factors, and show great potential for applications in biomedicine beyond. Notably, biodegradable SMPs offer a compelling advantage medical devices due their ability adapt within body's temperature range be absorbed tissues, reducing risks associated with permanent implants. While extrusion techniques have laid groundwork biomedicine, vat photopolymerization methods like stereolithography digital light processing are now at forefront, favored high resolution flexibility material design. However, search suitable materials these continues, current research focusing on developing systems meet both mechanical demands degradation profiles required applications. This review aims critically analyze advancements photopolymers, particularly elastomers, discuss challenges lie ahead clinical translation.

Language: Английский

Citations

7
Xolography for the Production of Polymeric Multimaterials DOI Creative Commons
Nathaniel Corrigan, Xichuan Li, Jin Zhang

et al.

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: 9(15)

Published: April 1, 2024

Abstract Three‐dimensional (3D) printed multimaterials have quickly grown in interest as researchers aim to mimic the material chemistry and geometric complexity of natural materials. While some exceptional multimaterial 3D printing systems been developed, there is still a shortfall scope production these More recently, introduction xolography single‐step, auxiliary‐free additive manufacturing approach has enabled rapid materials with geometries inaccessible via other techniques. However, previous required resins high transparency for effective formation, formation not demonstrated. In this work, reversible deactivation radical polymerization used resin composition, viscosity, processing parameters are explored develop new capable application visible light‐mediated xolography. After optimization, non‐transparent containing thiocarbonylthio species applied manufacture geometrically complex, The resulting polymer network can be functionalized embedded moieties impart functionality Finally, unique capabilities demonstrated featuring chemically disparate domains 3D‐spatial control. This two‐step overprinting operationally simple provides avenue polymeric printing.

Language: Английский

Citations

6
Volumetric Additive Manufacturing of SiOC by Xolography DOI
Kai Huang, Giorgia Franchin, Paolo Colombo

et al.

Small, Journal Year: 2024, Volume and Issue: 20(37)

Published: May 10, 2024

Additive manufacturing (AM) of ceramics has significantly contributed to advancements in ceramic fabrication, solving some the difficulties conventional processing and providing additional possibilities for structure function components. However, defects induced by layer-by-layer approach on which traditional AM techniques are based still constitute a challenge address. This study presents volumetric SiOC from preceramic polymer using xolography, linear process that allows avoid staircase effect typical other vat photopolymerization techniques. Besides optimizing trade-off between content transmittance, pore generator is introduced create transient channels gas release before decomposition organic constituents moieties, resulting crack-free solid structures even at low yield. Formulation optimization alleviated sinking printed parts during printing prevented shape distortion. Complex porous with smooth surface sharp features fabricated under optimized parameters. work provides new method µm/mm scale high quality large geometry variety an efficient way, opening possibility applications fields such as micromechanical systems microelectronic

Language: Английский

Citations

4
Advances in materials and technologies for digital light processing 3D printing DOI Creative Commons
Jisoo Nam, Miso Kim

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 4, 2024

Abstract Digital light processing (DLP) is a projection-based vat photopolymerization 3D printing technique that attracts increasing attention due to its high resolution and accuracy. The layer-by-layer deposition in DLP uses precise control cure photopolymer resin quickly, providing smooth surface finish the uniform layer curing process. Additionally, extensive material selection printing, notably including existing photopolymerizable materials, presents significant advantage compared with other techniques limited choices. Studies can be categorized into two main domains: material-level system-level innovation. Regarding innovations, development of photocurable resins tailored rheological, photocuring, mechanical, functional properties crucial for expanding application prospects technology. In this review, we comprehensively review state-of-the-art advancements focusing on innovations centered particularly various smart materials 4D addition piezoelectric ceramics their composites applications DLP. discuss recyclable promote sustainable manufacturing practices. are also delineated, recent progress multi-materials DLP, grayscale AI-assisted related developments. We highlight current challenges propose potential directions future development. Exciting areas such as creation stimuli-responsive functionality, ceramic AI-enhanced still nascent stages. By exploring concepts like recycling technology, integration these aspects unlock opportunities driven by Through aim stimulate further interest encourage active collaborations advancing systems, fostering dynamic field. Graphical abstract

Language: Английский

Citations

4
Antagonistic Two‐Color Control of Polymer Network Formation DOI Creative Commons
Arnau Marco, Marc Villabona, Tugce Nur Eren

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Offering high spatiotemporal resolution, dual wavelength‐controlled soft matter network formation paves the way to advanced printing techniques with optimized performance. One of most promising approaches is antagonistic control covalent bond‐forming reactions two colors light, where photoexcitation one wavelength induces a photochemical reaction, while irradiation other ceases it in presence first color. Herein, we combine photoactivatable diene precursor and photoswitchable dienophile, establishing dual‐wavelength‐gated cycloaddition reaction capable controlling polymer crosslinking. Upon incorporation into methacrylate copolymer synthesis difunctional dienophile cross‐linker, selective promoted under sole UV illumination, can be efficiently suppressed simultaneous redlight irradiation. Critically, methodology used for preparation solid materials two‐color their cross‐linking status, ultimately allowing fabrication spatially patterned films.

Language: Английский

Citations

0
Xolography for Biomedical Applications: Dual‐Color Light‐Sheet Printing of Hydrogels With Local Control Over Shape and Stiffness DOI Creative Commons

Lena Stoecker,

Gerardo Cedillo‐Servín, Niklas F. König

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Abstract Current challenges in tissue engineering include creation of extracellular environments that support and interact with cells using biochemical, mechanical, structural cues. Spatial control over these cues is currently limited due to a lack suitable fabrication techniques. This study introduces Xolography, an emerging dual‐color light‐sheet volumetric printing technology, achieve mechanical features for hydrogel‐based photoresins at micro‐ macroscale while within minutes. A water‐soluble photoswitch photoinitiator system library naturally‐derived, synthetic, thermoresponsive hydrogels Xolography are proposed. Centimeter‐scale, 3D constructs positive 20 µm negative ≈100 fabricated properties (compressive moduli 0.2 kPa–6.5 MPa). Notably, switching from binary grayscaled light projection enables spatial stiffness (0.2–16 kPa). As proof concept, leveraged introduce reversible anisotropic shape changes beyond isometric shrinkage. viable cell aggregates finally demonstrated, laying the foundation cell‐laden dynamic, cell‐instructive tunable fast one‐step process. Overall, innovations unlock unique possibilities across multiple biomedical applications.

Language: Английский

Citations

0