Advances in medical diagnosis, treatment, and care (AMDTC) book series,
Год журнала:
2024,
Номер
unknown, С. 383 - 408
Опубликована: Дек. 17, 2024
Spatial
transcriptomics
is
a
powerful
tool
in
biomedical
research
that
enables
the
analysis
of
gene
expression
patterns
within
their
spatial
context.
By
providing
insights
into
organisation
expression,
this
technology
can
help
address
limitations
design
synthetic
circuits
controlled
manner.
In
cancer
biology,
combining
with
other
approaches
allows
researchers
to
gain
deeper
understanding
spatiotemporal
dynamics
regulation.
This
method
has
significant
potential
for
revealing
tissue
architecture
and
cellular
heterogeneity,
which
complements
circuits.
study
explores
advantages
circuit
analysis,
focusing
on
integrating
these
techniques
understand
better
temporal
regulation
genes
tissues.
Abstract
Extracellular
vesicles
(EVs)
are
heterogeneous
membrane-like
secreted
by
living
cells
that
involved
in
many
physiological
and
pathological
processes
act
as
intermediaries
of
intercellular
communication
molecular
transfer.
Recent
studies
have
shown
EVs
from
specific
sources
regulate
tissue
repair
regeneration
delivering
proteins,
lipids,
nucleic
acids
to
target
signaling
molecules.
Nanotechnology
breakthroughs
facilitated
the
development
exploration
engineered
for
repair.
Enhancements
through
gene
editing,
surface
modification,
content
modification
further
improved
their
therapeutic
efficacy.
This
review
summarizes
potential
regeneration,
mechanisms
action,
research
progress
regenerative
medicine.
highlights
design
logic
typical
examples
explores
prospects
The
aim
this
is
provide
new
insights
into
applications,
thereby
expanding
use
Theranostics,
Год журнала:
2025,
Номер
15(5), С. 1715 - 1740
Опубликована: Янв. 2, 2025
Chronic
liver
diseases,
primarily
metabolic
dysfunction-associated
steatotic
disease
(MASLD),
and
alcoholic
(MetALD),
viral
hepatitis,
can
lead
to
fibrosis,
cirrhosis,
cancer.
Hepatic
stellate
cell
(HSC)
activation
plays
a
central
role
in
the
development
of
myofibroblasts
fibrogenesis
chronic
diseases.
However,
HSC
is
influenced
by
complex
microenvironments
within
liver,
which
are
largely
shaped
interactions
between
HSCs
various
other
types.
Changes
phenotypes
mechanisms
involve
glucose,
lipid,
cholesterol
metabolism,
oxidative
stress,
unfolded
protein
response
(UPR),
autophagy,
ferroptosis,
senescence,
nuclear
receptors.
Clinical
interventions
targeting
these
pathways
have
shown
promising
results
addressing
inflammation
as
well
modulating
glucose
lipid
metabolism
stress
responses.
Therefore,
comprehensive
understanding
presents
opportunities
for
novel
therapeutic
approaches
aimed
at
halting
or
even
reversing
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
175, С. 116702 - 116702
Опубликована: Май 11, 2024
In
recent
years,
nanoparticles
have
been
broadly
utilized
in
various
drugs
delivery
formulations.
Nanodelivery
systems
shown
promise
solving
problems
associated
with
the
distribution
of
hydrophobic
and
promoted
accumulation
nanomedicines
circulation
or
organs.
However,
injection
dose
(NPs)
is
much
greater
than
that
needed
by
diseased
tissues
other
words,
most
NPs
are
localized
off-target
do
not
reach
desired
tissue
With
rapid
development
biodegradable
biosafety
nanomaterials,
nanovectors
represent
assurance
safety.
effects
also
induce
concerns
about
application
NPs,
especially
gene
editing
tools.
Therefore,
a
complete
understanding
biological
responses
to
body
will
clearly
guide
design
targeted
NPs.
The
different
properties
nanodelivery
may
diverse
interactions
between
carriers
this
review,
we
describe
relationship
liver,
influenced
organ
systemic
administration
nanoplatforms.
Various
transport
vehicles
adopted
multiple
strategies
for
cells
homeostasis
liver
liver.
Additionally,
provide
novel
strategy
treating
incurable
diseases.
appearance
has
profoundly
improved
Journal of Cellular Physiology,
Год журнала:
2024,
Номер
239(11)
Опубликована: Июль 22, 2024
The
endoplasmic
reticulum
(ER)
is
crucial
for
protein
quality
control,
and
disruptions
in
its
function
can
lead
to
various
diseases.
ER
stress
triggers
an
adaptive
response
called
the
unfolded
(UPR),
which
either
restore
cellular
homeostasis
or
induce
cell
death.
Melatonin,
a
safe
multifunctional
compound,
shows
promise
controlling
could
be
valuable
therapeutic
agent
managing
UPR.
By
regulating
mitochondrial
functions,
melatonin
helps
maintain
via
reduction
of
oxidative
stress,
inflammation,
apoptosis.
Melatonin
directly
indirectly
interfere
with
ER-associated
sensors
downstream
targets
UPR,
impacting
death,
autophagy,
molecular
repair,
among
others.
Crucially,
this
review
explores
mechanistic
role
on
diseases
including
liver
damage,
neurodegeneration,
reproductive
disorders,
pulmonary
disease,
cardiomyopathy,
insulin
resistance,
renal
dysfunction,
cancer.
Interestingly,
while
it
alleviates
burden
most
pathological
contexts,
paradoxically
stimulate
cancer
cells,
highlighting
intricate
involvement
homeostasis.
With
numerous
successful
studies
using
vivo
vitro
models,
continuation
clinical
trials
imperative
fully
explore
melatonin's
potential
these
conditions.
Cell Death and Disease,
Год журнала:
2024,
Номер
15(5)
Опубликована: Май 14, 2024
Abstract
Fibrosis
is
a
reparative
and
progressive
process
characterized
by
abnormal
extracellular
matrix
deposition,
contributing
to
organ
dysfunction
in
chronic
diseases.
The
tumor
suppressor
p53
(p53),
known
for
its
regulatory
roles
cell
proliferation,
apoptosis,
aging,
metabolism
across
diverse
tissues,
appears
play
pivotal
role
aggravating
biological
processes
such
as
epithelial-mesenchymal
transition
(EMT),
senescence.
These
are
closely
intertwined
with
the
pathogenesis
of
fibrotic
disease.
In
this
review,
we
briefly
introduce
background
specific
mechanism
p53,
investigate
fibrosis,
further
discuss
p53’s
relationship
fibrosis
affecting
kidney,
liver,
lung,
heart.
summary,
targeting
represents
promising
innovative
therapeutic
approach
prevention
treatment
fibrosis.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(44), С. 59777 - 59788
Опубликована: Окт. 28, 2024
This
study
aims
to
explore
the
efficacy
and
safety
of
macrophage
membrane-coated
nanoparticles
for
delivery
natamycin
(NAT)
in
therapy
fungal
keratitis
(FK).
Macrophage
membranes
were
isolated
identified
by
immunofluorescence
staining
(IFS).
NAT
was
encapsulated
into
poly(lactic-co-glycolic
acid)
(PLGA).
Fungal
stimulated
(M1)
or
unstimulated
(M)
separately
mixed
sonicated
with
PLGA
nanoparticles.
The
biocompatible
(PLGA-NAT,
PLGA-NAT@M,
PLGA-NAT@M1)
characterized
zeta-sizer
analysis,
transmission
electron
microscopy
(TEM),
Western
blot.
Drug
encapsulation
loading
efficiency
release
detected
ultraviolet
spectrophotometry.
cytotoxicity,
ocular
surface
toxicity
irritability,
systemic
different
concentrations
assessed.
In
vitro,
we
examined
antifungal
properties
eye
retention
time,
drug
release,
curative
effects
on
FK
evaluated
vitro
vivo.
IFS
results
showed
separation
membrane
nucleus.
prepared
had
a
typical
"core–shell"
structure
uniform
nanometer
size,
proteins
retained
allowing
exert
functional
macrophage.
efficiencies
PLGA-NAT@M
PLGA-NAT@M1
7.6
6.7%,
respectively.
51.2
41.5%,
could
gradually
reduce
clearance
surface.
enhanced
activity
PLGA-NAT.
Furthermore,
coated
increased
biocompatibility
decreased
corneal
vivo,
significantly
alleviated
severity
FK.
PLGA@M
PLGA@M1
reduced
protein
levels
inflammatory
cytokines
after
stimulation.
has
good
physical
biosafety.
It
evade
clearance,
gradually,
achieve
high
anti-inflammatory
clinically
have
application
potential
treatment
Liver
fibrosis
is
characterized
by
the
excessive
accumulation
of
extracellular
matrix
proteins
primarily
produced
activated
hepatic
stellate
cells
(HSCs).
The
activation
HSCs
plays
a
pivotal
role
in
driving
progression
liver
fibrosis.
Achieving
specific
targeted
delivery
antifibrotic
agents
toward
remains
formidable
challenge.
Here,
we
developed
an
HSC
membrane-camouflaged
nanosystem,
named
HSC-PLGA-BAY,
for
precise
antifibrosis
agent
BAY
11-7082
to
treatment
designed
HSC-PLGA-BAY
nanosystem
exhibited
selective
targeting
HSCs,
with
internalization
mediated
homologous
cell
adhesion
molecules
from
membrane,
namely
integrins
and
N-cadherin.
Furthermore,
our
findings
demonstrate
that
HSC-PGA-BAY
significantly
increased
apoptosis
ameliorated
bile
duct
ligation
(BDL)-induced
fibrotic
mice
model.
Collectively,
HSCs-targeted
therapeutic
platform
holds
promising
potential
as
effective
strategy
treatment.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 9, 2025
To
investigate
the
association
between
overt
hepatic
encephalopathy
(OHE)
and
liver
pathology
after
transjugular
intrahepatic
portosystemic
shunt
(TIPS)
creation
in
cirrhotic
patients.
From
July
2015
to
April
2024,
73
patients
from
4
hospitals
China
who
received
TIPS
biopsy
were
retrospectively
enrolled
this
study.
Based
on
whether
OHE
occurred
within
3
months
creation,
categorized
into
(n
=
29)
non-OHE
44)
groups.
The
was
assessed
by
hematoxylin-eosin
(H&E),
Sirius
red
staining,
immunohistochemistry,
immunofluorescence.
Liver
H&E
staining
showed
typical
features
of
cirrhosis
(including
disordered
structure
pseudolobule
formation)
all
No
marked
difference
observed
extracellular
matrix
(ECM)
deposition
However,
group
had
a
higher
level
systemic
inflammation
than
group.
And
there
strong
correction
macrophage
infiltration
serum
inflammatory
indicators.
Additionally,
more
neovascularization,
which
consistent
with
inflammation.
emergence
is
closely
associated
pathology,
especially
angiogenesis,
but
not
ECM
deposition.
