Evaluating the Impact of the Membrane Thickness on the Function of the Intramembrane Protease GlpG DOI Creative Commons
Oskar Engberg, Anjana V. Mathath,

Viola Döbel

и другие.

Biophysical Journal, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Cellular membranes exhibit a huge diversity of lipids and membrane proteins that differ in their properties chemical structure. Cells organize these molecules into distinct compartments characterized by specific lipid profiles hydrophobic thicknesses the respective domains. If mismatch occurs between protein surrounding lipids, there can be functional consequences such as reduced activity. This phenomenon has been extensively studied for single-pass transmembrane proteins, rhodopsin, small polypeptides gramicidin. Here, we investigate E. coli rhomboid intramembrane protease GlpG model to systematically explore impact thickness on We used fully saturated 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) 1,2-dimyristoyl-sn-glycero-3-phosphocholine(DMPC) altered varying cholesterol content. Physical parameters were determined

Язык: Английский

Phospholipid-derived lysophospholipids in (patho)physiology DOI Creative Commons
Patricia Prabutzki, Jürgen Schiller,

Kathrin M. Engel

и другие.

Atherosclerosis, Год журнала: 2024, Номер 398, С. 118569 - 118569

Опубликована: Авг. 23, 2024

Phospholipids (PL) are major components of cellular membranes and changes in PL metabolism have been associated with the pathogenesis numerous diseases. Lysophosphatidylcholine (LPC) particular, is a comparably abundant component oxidatively damaged tissues. LPC originates from cleavage phosphatidylcholine (PC) by phospholipase A

Язык: Английский

Процитировано

6

Molecules in the Serotonin-Melatonin Synthesis Pathway Have Distinct Interactions with Lipid Membranes DOI Creative Commons
Oskar Engberg, Debsankar Saha Roy, Paweł Krupa

и другие.

The Journal of Physical Chemistry B, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

The neurotransmitter serotonin is involved in physiological processes such as appetite, sleep, and mood diseases anxiety depression. Traditionally, the effects of were thought to be initiated by binding its target transmembrane receptors. It also known that can bind directly membrane with high affinity modulate lipid dynamics, lateral segregation lipids, vesicular association, protein activity. We investigated if other small molecules metabolic pathway, some which are signaling while others not, have similar modulating effects. Therefore, we examined several metabolites: 5-hydroxytryptophan (5-HTP), serotonin, N-acetylserotonin (NAS), melatonin model membranes mimicking synaptic membranes. Using 2H NMR spectroscopy deuterated 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC), observed all metabolites disorder membrane-mimicking largest disordering effect was for NAS smallest tryptophan. fluorescence correlation spectroscopy, it found only promotes association did not. Furthermore, differed their distribution employing solid state 1H magic angle spinning nuclear Overhauser enhancement (NOESY) experiments simple POPC Similar results obtained mimics using molecular dynamics simulations. In conclusion, causal between modulation remains elusive, this study suggests small-molecule drugs drastic biological mediated through membrane. finding changes structure lead very different distributions possibility developing future.

Язык: Английский

Процитировано

0

Photoswitchable phospholipids for the optical control of membrane processes, protein function, and drug delivery DOI Creative Commons
Stefanie D. Pritzl, Johannes Morstein,

Nikolaj A. Pritzl

и другие.

Communications Materials, Год журнала: 2025, Номер 6(1)

Опубликована: Апрель 1, 2025

Recent insights into the function and composition of cell membranes have transformed our understanding from primarily viewing these structures as passive barriers to recognizing them dynamic entities actively involved in many cellular functions. This review highlights advances photopharmacology phospholipids, emphasizing particular role diacylglycerophospholipids impact their polymorphic nature on synthetic membrane properties metabolic processes. We explore photoswitchable diacylglycerophospholipids, termed 'photolipids', which permit precise, reversible modifications via light-induced isomerization. The ability optically switch phospholipid has potential applications controlling dynamics, protein function, signaling pathways, offers promising strategies for drug delivery treatment diseases. Developments azobenzene hemithioindigo based photolipids are discussed, underscoring utility biomedical biomaterial science due unique photophysical properties. Cell that Review recent exploring how they used applications.

Язык: Английский

Процитировано

0

Precise control of transmembrane current via regulating bionic lipid membrane composition DOI Creative Commons
Zhiwei Shang, Jing Zhao, Mengyu Yang

и другие.

Science Advances, Год журнала: 2024, Номер 10(35)

Опубликована: Авг. 30, 2024

The transport of ions through biological ion channels is regulated not only by their structural characteristics but also the composition phospholipid membrane, which serves as a carrier for nanochannels. Inspired modulation currents lipid membrane composition, exemplified activation K

Язык: Английский

Процитировано

2

Brief Comparison of the Efficacy of Cationic and Anionic Liposomes as Nonviral Delivery Systems DOI Creative Commons

Carlos Ochoa-Sánchez,

Ericka Rodríguez-León, Ramón Íñiguez-Palomares

и другие.

ACS Omega, Год журнала: 2024, Номер 9(47), С. 46664 - 46678

Опубликована: Ноя. 13, 2024

In recent decades, the development and application of nonviral vectors, such as liposomes lipidic nanoparticles, for gene therapy drug delivery have seen substantial progress. The interest in physicochemical properties structures complexes liposome/DNA liposome/RNA is due to their potential substitute viruses carriers drugs or genetic material into cells with minimal cytotoxicity, which could lead use therapy. Initially, cationic were utilized DNA vectors; subsequently, different molecules, polymers, incorporated enhance transfection efficiency. Additionally, liposome/protein been developed vectors treatment diseases. most relevant internalization pathways these few results obtained using targeted nontargeted are discussed below. high cytotoxicity represents a significant challenge delivery. Anionic offer promising alternative address limitations conventional liposomes, including immune response, short circulation time, low toxicity. This review will discuss advantages novel anionic liposome-based systems that emerged result. advent designs manufacturing techniques has facilitated innovative systems, designated lipid nanoparticles (LNPs), serve highly efficacious regulators system.

Язык: Английский

Процитировано

2

Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations DOI Creative Commons
Pantelitsa Dimitriou, Jin Li, William David Jamieson

и другие.

Communications Chemistry, Год журнала: 2024, Номер 7(1)

Опубликована: Июнь 1, 2024

Abstract Droplet Interface Bilayers (DIBs) constitute a commonly used model of artificial membranes for synthetic biology research applications. However, their practical use is often limited by requirement to be surrounded oil. Here we demonstrate in-situ bilayer manipulation submillimeter, hydrogel-encapsulated droplet interface bilayers (eDIBs). Monolithic, Cyclic Olefin Copolymer/Nylon 3D-printed microfluidic devices facilitated the eDIB formation through high-order emulsification. By exposing capsules varying lysophosphatidylcholine (LPC) concentrations, investigated interaction lysolipids with three-dimensional DIB networks. Micellar LPC concentrations triggered bursting encapsulated networks, while at lower network endured structural changes, precisely affecting membrane dimensions. This chemically-mediated enclosed, 3D-orchestrated mimics, facilitates exploration readily accessible compartmentalized cellular machinery. Collectively, droplet-based construct can pose as chemically responsive soft material studying mechanics, and drug delivery, controlling cargo release from cell chassis.

Язык: Английский

Процитировано

1

Extreme Makeover: The Incredible Cell Membrane Adaptations of Extremophiles to Harsh Environments DOI
Archita Maiti, Shakkira Erimban, Snehasis Daschakraborty

и другие.

Chemical Communications, Год журнала: 2024, Номер 60(75), С. 10280 - 10294

Опубликована: Янв. 1, 2024

The existence of life beyond Earth has long captivated humanity, and the study extremophiles-organisms surviving thriving in extreme environments-provides crucial insights into this possibility. Extremophiles overcome severe challenges such as enzyme inactivity, protein denaturation, damage cell membrane by adopting several strategies. This feature article focuses on molecular strategies extremophiles use to maintain membrane's structure fluidity under external stress. Key include homeoviscous adaptation (HVA), involving regulation lipid composition, osmolyte-mediated (OMA), where small organic molecules protect Proteins also have direct indirect roles protecting membrane. Examining survival provides scientists with how can adapt persist harsh conditions, shedding light origins life. examines HVA OMA their mechanisms maintaining stability, emphasizing our contributions field. It a brief overview proteins concludes recommendations for future research directions.

Язык: Английский

Процитировано

0

Regulation of Transmembrane Current through Modulation of Biomimetic Lipid Membrane Composition DOI

Zhiwei Shang,

Jing Zhao, Mengyu Yang

и другие.

Faraday Discussions, Год журнала: 2024, Номер unknown

Опубликована: Авг. 23, 2024

Ion transport through biological channels is influenced not only by the structural properties of themselves but also composition phospholipid membrane, which acts as a scaffold for these nanochannels. Drawing inspiration from how lipid membrane modulates ion currents, seen in activation K

Язык: Английский

Процитировано

0

Effects of a Serotonergic Psychedelic on the Lipid Bilayer DOI
Debsankar Saha Roy, Ankit Singh, Vidita A. Vaidya

и другие.

ACS Chemical Neuroscience, Год журнала: 2024, Номер 15(21), С. 4066 - 4074

Опубликована: Окт. 21, 2024

Serotonergic psychedelics, known for their hallucinogenic effects, have attracted interest due to ability enhance neuronal plasticity and potential therapeutic benefits. Although psychedelic-enhanced neuroplasticity is believed require activation of 5-hydroxytryptamine (serotonin) 2A receptors (5-HT2ARs), serotonin itself less effective in promoting such plasticity. Also, the psychoplastogenic effects these molecules correlate with lipophilicity, leading suggestions that they act by influencing intracellular receptors. However, lipophilicity also implies a significant quantity lipids accumulated lipid bilayer, potentially altering physical properties membrane. Here, we probe whether serotonergic psychedelic 2,5-dimethoxy-4-iodoamphetamine (DOI) can affect artificial bilayers if processes as membrane fusion. Solid-state NMR spectroscopy shows DOI strongly induces disorder acyl chains. Atomic force microscopy it shrink ordered domains biphasic bilayer reduce needed form nanopores Fluorescence correlation promote vesicle association, total internal fluorescence enhances fusion supported bilayer. While has recently been shown cause similar more than two orders magnitude potent evoking these. Our results suggest receptor-independent psychedelics on membranes may contribute biological actions, especially those remodeling,

Язык: Английский

Процитировано

0

Evaluating the Impact of the Membrane Thickness on the Function of the Intramembrane Protease GlpG DOI Creative Commons
Oskar Engberg, Anjana V. Mathath,

Viola Döbel

и другие.

Biophysical Journal, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Cellular membranes exhibit a huge diversity of lipids and membrane proteins that differ in their properties chemical structure. Cells organize these molecules into distinct compartments characterized by specific lipid profiles hydrophobic thicknesses the respective domains. If mismatch occurs between protein surrounding lipids, there can be functional consequences such as reduced activity. This phenomenon has been extensively studied for single-pass transmembrane proteins, rhodopsin, small polypeptides gramicidin. Here, we investigate E. coli rhomboid intramembrane protease GlpG model to systematically explore impact thickness on We used fully saturated 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) 1,2-dimyristoyl-sn-glycero-3-phosphocholine(DMPC) altered varying cholesterol content. Physical parameters were determined

Язык: Английский

Процитировано

0