Critical Reviews in Analytical Chemistry,
Год журнала:
2023,
Номер
54(7), С. 2599 - 2617
Опубликована: Март 20, 2023
Food
safety
is
one
of
the
greatest
public
problems
occurring
around
world.
Chemical,
physical,
and
microbiological
hazards
could
lead
to
food
problems,
which
might
occur
at
all
stages
supply
chain.
To
tackle
protect
consumer
health,
specific,
accurate,
rapid
diagnosis
techniques
meeting
various
requirements
are
imperative
measures
ensure
safety.
CRISPR-Cas
system,
a
novel
emerging
technology,
effectively
repurposed
in
(bio)sensing
has
shown
tremendous
capability
develop
on-site
portable
diagnostic
methods
with
high
specificity
sensitivity.
Among
numerous
existing
CRISPR/Cas
systems,
CRISPR/Cas13a
CRISPR/Cas12a
extensively
employed
design
biosensors,
owing
their
ability
cleave
both
non-target
target
sequences.
However,
limitation
hindered
its
progress.
Nowadays,
nucleic
acid
aptamers
recognized
for
high-affinity
characteristics
analytes
incorporated
into
systems.
With
benefits
reproducibility,
durability,
portability,
facile
operation,
cost-effectiveness,
CRISPR/Cas-based
aptasensing
approaches
an
ideal
choice
fabricating
highly
specific
point-of-need
analytical
tools
enhanced
response
signals.
In
current
study,
we
explore
some
most
recent
progress
CRISPR/Cas-mediated
aptasensors
detecting
risk
factors
including
veterinary
drugs,
pesticide
residues,
pathogens,
mycotoxins,
heavy
metals,
illegal
additives,
other
contaminants.
The
nanomaterial
engineering
support
also
signified
achieve
hopeful
perspective
provide
new
straightforward
test
kits
toward
trace
amounts
different
contaminants
encountered
samples.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(17)
Опубликована: Янв. 30, 2023
Abstract
Polymerase
chain
reaction
(PCR)‐based
nucleic
acid
testing
has
played
a
critical
role
in
disease
diagnostics,
pathogen
surveillance,
and
many
more.
However,
this
method
requires
long
turnaround
time,
expensive
equipment,
trained
personnel,
limiting
its
widespread
availability
diagnostic
capacity.
On
the
other
hand,
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
technology
recently
demonstrated
capability
for
detection
with
high
sensitivity
specificity.
CRISPR‐mediated
biosensing
holds
great
promise
revolutionizing
procedures
developing
point‐of‐care
diagnostics.
This
review
focuses
on
recent
developments
both
fundamental
CRISPR
biochemistry
CRISPR‐based
techniques.
Four
ongoing
research
hotspots
molecular
diagnostics‐target
preamplification‐free
detection,
microRNA
(miRNA)
testing,
non‐nucleic‐acid
SARS‐CoV‐2
detection‐are
also
covered.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2022,
Номер
21(4), С. 3770 - 3798
Опубликована: Июль 1, 2022
Abstract
Food
safety
is
one
of
the
biggest
public
issues
occurring
around
world.
Microbiological,
chemical,
and
physical
hazards
can
lead
to
food
issues,
which
may
occur
at
all
stages
supply
chain.
In
order
tackle
safeguard
consumer
health,
rapid,
accurate,
specific,
field‐deployable
detection
methods
meeting
diverse
requirements
are
imperative
measures
for
assurance.
CRISPR‐Cas
system,
a
newly
emerging
technology,
has
been
successfully
repurposed
in
biosensing
demonstrated
huge
potential
establish
conceptually
novel
with
high
sensitivity
specificity.
This
review
focuses
on
CRISPR‐Cas–based
its
current
status
specifically
inspection.
We
firstly
illustrate
pending
problems
summarize
popular
methods.
then
describe
applications
Finally,
challenges
futuristic
opportunities
proposed
discussed.
Generally
speaking,
still
unsatisfactory
some
ways
such
as
being
time‐consuming,
displaying
unmet
specificity
standards,
there
comparative
paucity
multiplexed
testing
POCT.
Recent
studies
have
shown
that
an
innovative
fast‐expanding
could
make
up
shortcomings
existing
or
even
replace
them.
To
sum
up,
implementation
integration
other
techniques
promising
desirable,
expected
provide
“customized”
“smart”
inspection
coming
future.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Сен. 5, 2023
Abstract
Cas12a,
a
CRISPR-associated
protein
complex,
has
an
inherent
ability
to
cleave
DNA
substrates
and
is
utilized
in
diagnostic
tools
identify
molecules.
We
demonstrate
that
multiple
orthologs
of
Cas12a
activate
trans-cleavage
the
presence
split
activators.
Specifically,
PAM-distal
region
crRNA
recognizes
RNA
targets
provided
PAM-proximal
seed
target.
Our
method,
Split
Activator
for
Highly
Accessible
Analysis
(SAHARA),
detects
picomolar
concentrations
without
sample
amplification,
reverse-transcription,
or
strand-displacement
by
simply
supplying
short
sequence
complementary
region.
Beyond
detection,
SAHARA
outperforms
wild-type
CRISPR-Cas12a
specificity
towards
point-mutations
can
detect
pooled
crRNA/Cas12a
arrays
via
distinct
DNAs.
In
conclusion,
simple,
yet
powerful
nucleic
acid
detection
platform
based
on
be
applied
multiplexed
fashion
potentially
expanded
other
CRISPR-Cas
enzymes.
Sensors and Actuators A Physical,
Год журнала:
2024,
Номер
376, С. 115625 - 115625
Опубликована: Июнь 24, 2024
The
integration
of
CRISPR
technology
with
microfluidic-based
biosensors
has
greatly
expanded
its
applications
in
medicine
and
molecular
biology.
This
combination
offers
enhanced
sensitivity
selectivity
medical
diagnostics.
CRISPR-powered
microfluidics
enables
the
accurate
quantification
DNA
RNA
associated
diseases
such
as
cancer,
viral
infections,
bacterial
diseases.
precise
targeting
capabilities
allow
for
detection
specific
genetic
sequences,
aiding
early
diagnosis,
disease
monitoring,
treatment
assessment.
improves
specificity
by
leveraging
programmable
nature
coupling
it
signal
readouts.
However,
challenges
assay
optimization,
standardization,
device
fabrication
need
to
be
addressed.
Additionally,
complexity
presents
potential
limitations
terms
off-target
effects
unintended
modifications.
Overall,
holds
tremendous
diagnostics,
but
further
research
development
are
required
fully
exploit
benefits
clinical
settings.
Military Medical Research,
Год журнала:
2022,
Номер
9(1)
Опубликована: Март 18, 2022
Abstract
Traditional
diagnostic
strategies
for
infectious
disease
detection
require
benchtop
instruments
that
are
inappropriate
point-of-care
testing
(POCT).
Emerging
microfluidics,
a
highly
miniaturized,
automatic,
and
integrated
technology,
potential
substitute
traditional
methods
in
performing
rapid,
low-cost,
accurate,
on-site
diagnoses.
Molecular
diagnostics
widely
used
microfluidic
devices
as
the
most
effective
approaches
pathogen
detection.
This
review
summarizes
latest
advances
microfluidics-based
molecular
diseases
from
academic
perspectives
industrial
outlooks.
First,
we
introduce
typical
on-chip
nucleic
acid
processes,
including
sample
preprocessing,
amplification,
signal
read-out.
Then,
four
categories
of
platforms
compared
with
respect
to
features,
merits,
demerits.
We
further
discuss
application
digital
assay
absolute
quantification.
Both
classic
recent
commercial
summarized
proof
current
market
status.
Finally,
propose
future
directions
diagnosis.
Advanced Materials Technologies,
Год журнала:
2021,
Номер
6(12)
Опубликована: Июль 19, 2021
CRISPR
(Clustered
regularly
interspaced
short
palindromic
repeats)-based
diagnostic
technologies
have
emerged
as
a
promising
alternative
to
accelerate
delivery
of
SARS-CoV-2
molecular
detection
at
the
point
need.
However,
efficient
translation
CRISPR-diagnostic
field
application
is
still
hampered
by
dependence
on
target
amplification
and
reliance
fluorescence-based
results
readout.
Herein,
an
amplification-free
CRISPR/Cas12a-based
technology
for
RNA
presented
using
smartphone
camera
This
method,
termed
Cellphone-based
system
with
CRISPR/CAS-dependent
enzymatic
(CASCADE)
assay,
relies
mobile
phone
imaging
catalase-generated
gas
bubble
signal
within
microfluidic
channel
does
not
require
any
external
hardware
optical
attachments.
Upon
specific
reverse-transcribed
DNA/RNA
heteroduplex
(orf1ab)
ribonucleoprotein
complex,
transcleavage
collateral
activity
Cas12a
protein
Catalase:ssDNA
probe
triggers
system.
High
analytical
sensitivity
in
without
previous
(down
50
copies
µL
ACS Sensors,
Год журнала:
2023,
Номер
8(12), С. 4420 - 4441
Опубликована: Ноя. 18, 2023
CRISPR/Cas
system
is
becoming
an
increasingly
influential
technology
that
has
been
repositioned
in
nucleic
acid
detection.
A
preamplification
step
usually
required
to
improve
the
sensitivity
of
CRISPR/Cas-based
The
striking
biological
features
CRISPR/Cas,
including
programmability,
high
and
sequence
specificity,
single-base
resolution.
More
strikingly,
target-activated
trans-cleavage
could
act
as
a
biocatalytic
signal
transductor
amplifier,
thereby
empowering
it
potentially
perform
detection
without
step.
reports
such
work
are
on
rise,
which
not
only
scientifically
significant
but
also
promising
for
futuristic
end-user
applications.
This
review
started
with
introduction
methods
acids
diagnostics
(CRISPR-Dx).
Next,
we
objectively
discussed
pros
cons
steps
CRISPR-Dx.
We
then
illustrated
highlighted
recently
developed
strategies
CRISPR/Cas-powered
amplification-free
can
be
realized
through
uses
ultralocalized
reactors,
cascade
reactions,
ultrasensitive
systems,
or
others.
Lastly,
challenges
perspectives
were
proposed.
It
expected
this
makes
researchers
better
understand
current
emerging
field,
provides
insight
designing
novel
CRISPR-Dx
win
practicable
use
near
future.
