TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 170, С. 117431 - 117431
Опубликована: Ноя. 17, 2023
Язык: Английский
Journal of Hazardous Materials, Год журнала: 2023, Номер 454, С. 131485 - 131485
Опубликована: Апрель 25, 2023
Язык: Английский
Процитировано
62
Heliyon, Год журнала: 2024, Номер 10(4), С. e26179 - e26179
Опубликована: Фев. 1, 2024
CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 CRISPR/Cas3 utilize different mechanisms to detect or differentiate biological activities nucleotide sequences. Usually, CRISPR/Cas-based are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase amplification transcriptional technologies for effective diagnostics. Premised on these, many biosensors been developed acids viral bacterial pathogens in clinical samples, well other applications life sciences including biosecurity, food safety environmental assessment. Additionally, showed better specificity compared molecular diagnostic methods. In this review, we give an overview various methods highlight some advances their development components. We also discourse operational challenges advantages disadvantages systems. Finally, important considerations offered the improvement testing.
Язык: Английский
Процитировано
27
Environmental Technology & Innovation, Год журнала: 2024, Номер 34, С. 103625 - 103625
Опубликована: Апрель 4, 2024
Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.
Язык: Английский
Процитировано
22
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Янв. 3, 2025
Functional nucleic acids constitute a distinct category of that diverge from conventional acid amplification methodologies. They are capable forming intricate hybrid structures through Hoogsteen and reverse hydrogen bonding interactions between double-stranded single-stranded DNA, thereby broadening the spectrum DNA interactions. In recent years, functional DNA/RNA-based surface-enhanced Raman spectroscopy (SERS) has emerged as potent platform ultrasensitive multiplexed detection variety analytes interest. This review aims to elucidate operational principles several in SERS detection, including DNAzymes, G-quadruplexes, aptamers, CRISPR, origami etc., alongside design methodologies practical applications sensing. Initially, an overview is summarized encompassing structural attributes sensing mechanisms inherent diverse DNA/RNA. Following this, various innovative strategies for constructing acid-based sensors illustrated detail, aimed at improving present capabilities. A comprehensive summing up then conducted on these crucial fields, such disease diagnosis, environmental monitoring, food safety with particular focus sensitivity, specificity, analytical versatility. Finally, conclusive remarks offered along exploration existing challenges prospective avenues future research this developed field.
Язык: Английский
Процитировано
5
Trends in Food Science & Technology, Год журнала: 2023, Номер 138, С. 615 - 627
Опубликована: Июль 3, 2023
Язык: Английский
Процитировано
39
Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(37), С. 13577 - 13594
Опубликована: Сен. 1, 2023
Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.
Язык: Английский
Процитировано
33
TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 167, С. 117269 - 117269
Опубликована: Сен. 7, 2023
Язык: Английский
Процитировано
30
Food Frontiers, Год журнала: 2023, Номер 4(4), С. 2070 - 2080
Опубликована: Июнь 27, 2023
Abstract It is imperative to develop practicable pathogenic bacteria detection methods. We devised a biosensor for the ultrasensitive of Salmonella typhimurium ( S. typhi ), termed as SCOUT‐dCas9 (ultrasensitive, cross‐validating, on‐site, and dUal‐mode test using CRISPR/dCas9). Simply, species‐specific invA gene was amplified loop‐mediated isothermal amplification with biotinylated primer, which can be specifically “pulled down” by “antibody‐like” dCas9‐single guide RNA form ternary complex. SYBR Green I streptavidin‐modified alkaline phosphatase were used functionalize them generate fluorescent colorimetric signals, respectively. With this strategy, target could dexterously converted into bimodal signals that cross‐validated afford more reliable results. For both modes, able detect low 1 CFU/mL dynamic range from 10 9 CFU/mL. Lastly, had satisfactory selectivity capable detecting ‐contaminated real food samples. provides robust platform bacterial detection.
Язык: Английский
Процитировано
24
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 172, С. 117594 - 117594
Опубликована: Фев. 8, 2024
The precision and versatility of CRISPR-based techniques, combined with the advantages nucleic acid-based nanotechnology, hold great promise in transforming landscape molecular diagnostics. While significant progress has been made, current platforms primarly focus on acid detection. To expand applicability fully leverage offered by diagnostics, ongoing efforts explore strategies to develop CRISPR sensors capable detecting a diverse range analytes beyond acids. In addition, challenges still persist adaptation for point-of-care (POC) applications, involving concerns such as portability automation, well complexities associated multiplexing. Here, we provide detailed classification comprehensive discussion facilitating conversion non-nucleic target binding into CRISPR-powered outputs an emphasis their corresponding design principles. Furthermore, second part review outlines potential solutions seamlessly integrating these user-friendly rapid tests specifically tailored (POC).
Язык: Английский
Процитировано
17
Sensors and Actuators B Chemical, Год журнала: 2024, Номер 404, С. 135263 - 135263
Опубликована: Янв. 2, 2024
Язык: Английский
Процитировано
15