CRISPR-Cas9 Gene Therapy: Non-Viral Delivery and Stimuli-Responsive Nanoformulations

Molecules, Год журнала: 2025, Номер 30(3), С. 542 - 542

Опубликована: Янв. 24, 2025

The CRISPR-Cas9 technology, one of the groundbreaking genome editing methods for addressing genetic disorders, has emerged as a powerful, precise, and efficient tool. However, its clinical translation remains hindered by challenges in delivery efficiency targeting specificity. This review provides comprehensive analysis structural features, advantages, potential applications various non-viral stimuli-responsive systems, examining recent progress to emphasize address these limitations advance therapeutics. We describe how reports that nonviral vectors, including lipid-based nanoparticles, extracellular vesicles, polymeric gold mesoporous silica can offer diverse advantages enhance stability, cellular uptake, biocompatibility, based on their structures physio-chemical stability. also summarize nanoformulations, type vector, introduce precision control delivery. Stimuli-responsive nanoformulations are designed respond pH, redox states, external triggers, facilitate controlled targeted delivery, minimize off-target effects. insights our suggest future gene therapy technologies highlight systems CRISPR-Cas9’s efficacy, positioning them pivotal tools gene-editing therapies.

Язык: Английский

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Recent advances in near-infrared-II organic J-aggregates for bio-applications

Coordination Chemistry Reviews, Год журнала: 2025, Номер 528, С. 216379 - 216379

Опубликована: Янв. 4, 2025

Язык: Английский

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Advanced Near‐Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems

Advanced Science, Год журнала: 2020, Номер 7(8)

Опубликована: Фев. 27, 2020

Abstract Light‐based technique, including optical imaging and photoregulation, has become one of the most important tools for both fundamental research clinical practice, such as cell signal sensing, cancer diagnosis, tissue engineering, drug delivery, visual regulation, neuromodulation, disease treatment. In particular, low energy near‐infrared (NIR, 700–1700 nm) light possesses lower phototoxicity higher penetration depth in living systems compared with ultraviolet/visible light, making it a promising tool vivo applications. Currently, NIR light‐based photoregulation strategies have offered possibility to real‐time sense and/or modulate specific cellular events deep tissues subcellular accuracy. Herein, recent progress respect monitoring modulating spatiotemporal dynamics functions are summarized. applications techniques theranostics, regenerative medicine, neuroscience systematically introduced discussed. addition, challenges prospects sensing regulating comprehensively

Язык: Английский

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Multi-scale optoacoustic molecular imaging of brain diseases

European Journal of Nuclear Medicine and Molecular Imaging, Год журнала: 2021, Номер 48(13), С. 4152 - 4170

Опубликована: Фев. 16, 2021

Abstract The ability to non-invasively visualize endogenous chromophores and exogenous probes sensors across the entire rodent brain with high spatial temporal resolution has empowered optoacoustic imaging modalities unprecedented capacities for interrogating under physiological diseased conditions. This rapidly transformed microscopy (OAM) multi-spectral tomography (MSOT) into emerging research tools study animal models of diseases. In this review, we describe principles showcase recent technical advances that enable high-resolution real-time observations in preclinical models. addition, advanced molecular probe designs allow efficient visualization pathophysiological processes playing a central role variety neurodegenerative diseases, tumors, stroke. We outstanding challenges methodologies propose future outlook.

Язык: Английский

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Polydopamine‐Mediated Interfacial Functionalization of Implants for Accelerating Infected Bone Repair through Light‐Activatable Antibiosis and Carbon Monoxide Gas Regulated Macrophage Polarization

Advanced Functional Materials, Год журнала: 2022, Номер 32(27)

Опубликована: Апрель 9, 2022

Abstract Current treatments for implant‐associated infection remain unsatisfactory due to secondary and excessive inflammation, which impairs osseointegration. Herein, an interfacial functionalization strategy is proposed by the integration of a carbon monoxide gas (CO) nanogenerator on titanium implants, followed covalently grafting arginine‐glycine‐aspartic acid (RGD) polypeptide. Under near‐infrared light (NIR) irradiation, designed surface displays great light‐activatable antibiosis through CO‐potentiated mild photothermal therapy. Interestingly, functionalized exerts CO‐mediated anti‐inflammatory effect activating expression heme oxygenase (HO‐1), inducing down‐regulation p38 mitogen‐activated protein kinase (MAPK) nuclear factor kappa B (NFκB) (p50/p65). More importantly, combination CO delivery RGD immobilization drives polarization lipopolysaccharide (LPS)‐stimulated M1‐phenotype macrophages towards M2‐phenotype potential Janus 1/signal transducer activator transcription 6 (JAK1/STAT6) pathway, thereby remodeling damaged microenvironment into pro‐regenerative microenvironment. In rat model infection, effectively eliminates residual bacteria, alleviates accompanying inflammation mediates macrophage‐mediated immunomodulation, resulting in good osteogenesis. Together, these findings are first report therapeutic signal cascade immunomodulation‐osteogenic differentiation. The implant may serve as promising candidate replacement surgeries.

Язык: Английский

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Bioimaging with Upconversion Nanoparticles

Advanced Photonics Research, Год журнала: 2022, Номер 3(12)

Опубликована: Сен. 9, 2022

Bioimaging enables the spatiotemporal visualization of biological processes at various scales empowered by a range different imaging modalities and contrast agents. Upconversion nanoparticles (UCNPs) represent distinct type such agents with potential to transform bioimaging due their unique optical properties functional design flexibilities. This review explores discusses opportunities, challenges, limitations that UCNPs exhibit as probes highlights applications spatial dimensions ranging from single nanoparticle level cellular, tissue, whole animal imaging. Recent advancements in enabled UCNPs, including super‐resolution techniques multimodal methods are summarized, perspective on future UCNP‐based technologies research clinical translation is provided. may provide valuable resource for researchers interested exploring applying technologies.

Язык: Английский

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Gold Nanorods-Based Photothermal Therapy: Interactions Between Biostructure, Nanomaterial, and Near-Infrared Irradiation

Nanoscale Research Letters, Год журнала: 2022, Номер 17(1)

Опубликована: Июль 26, 2022

Gold nanorods (AuNRs) are ideal inorganic nanophotothermal agents with unique characteristics, including local surface plasmon resonance effects, easy scale preparation and functional modification, good biocompatibility. This review summarizes several recent advances in AuNRs-based photothermal therapy (PTT) research. Functionalized AuNRs have optimized biocompatibility targeting properties. The multifunctional nanoplatform composite structure meets the requirements for synergistic effects of PTT, photoacoustic imaging, other therapeutic methods. Photothermal (AuNRs-PTT) is widely used to treat tumors inflammatory diseases; its tumor-targeting, tumor metastasis inhibition, ablation abilities remarkable curative effects. An in-depth study living systems interactions between biological structure, nanomaterial, near-infrared irradiation could lay foundation further clinical research broad application PTT.

Язык: Английский

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Review of Noninvasive or Minimally Invasive Deep Brain Stimulation

Frontiers in Behavioral Neuroscience, Год журнала: 2022, Номер 15

Опубликована: Янв. 18, 2022

Brain stimulation is a critical technique in neuroscience research and clinical application. Traditional transcranial brain techniques, such as magnetic (TMS), direct current (tDCS), deep (DBS) have been widely investigated for decades. However, TMS tDCS poor spatial resolution penetration depth, DBS requires electrode implantation structures. These disadvantages limited the applications of these techniques. Owing to developments science technology, substantial advances noninvasive precise achieved by neuromodulation studies. Second-generation techniques that mainly rely on acoustic, electronic, optical, signals, focused ultrasound, temporal interference, near-infrared optogenetic, nanomaterial-enabled stimulation, offer great prospects neuromodulation. This review summarized mechanisms, development, applications, strengths challenges their development. We believe second-generation pave way disorder therapy.

Язык: Английский

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Pore-engineered nanoarchitectonics for cancer therapy

NPG Asia Materials, Год журнала: 2023, Номер 15(1)

Опубликована: Март 31, 2023

Abstract Nanoarchitectonics describes the integration of nanotechnology with other fields as a postnanotechnology concept that elevates it to material science. Based on this fundamental principle, we address pore-engineered nanoarchitectonics application targets for cancer therapy by combining basic descriptions and exemplifying applications in review. The initial two sections briefly summarize basics according classification based (i) porosity (ii) composition. Afterward, main application-oriented section—designing mesoporous therapy—is presented. Various types drug delivery systems, including nanoparticles nanocarriers, endogenous stimuli-responsive delivery, exogenous targeted are described. Importantly, clinical translation materials is further discussed. Mesoporous unique offer network cavities vehicles nanocarriers. Regarding developments allow be broadly used settings, there several challenges should solved their application. From perspective, tremendous processes development materials.

Язык: Английский

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Recent Advances on Peptide-Based Biosensors and Electronic Noses for Foodborne Pathogen Detection

Biosensors, Год журнала: 2023, Номер 13(2), С. 258 - 258

Опубликована: Фев. 11, 2023

Foodborne pathogens present a serious issue around the world due to remarkably high number of illnesses they cause every year. In an effort narrow gap between monitoring needs and currently implemented classical detection methodologies, last decades have seen increased development highly accurate reliable biosensors. Peptides as recognition biomolecules been explored develop biosensors that combine simple sample preparation enhanced bacterial in food. This review first focuses on selection strategies for design screening sensitive peptide bioreceptors, such isolation natural antimicrobial peptides (AMPs) from living organisms, by phage display use silico tools. Subsequently, overview state-of-the-art techniques peptide-based foodborne pathogen based various transduction systems was given. Additionally, limitations led innovative approaches food monitoring, electronic noses, promising alternatives. The receptors noses is growing field recent advances are presented. All these alternatives with sensitivity, low cost rapid response, some them potential portable devices on-site analyses.

Язык: Английский

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