Nanoplasmonic amplification in microfluidics enables accelerated colorimetric quantification of nucleic acid biomarkers from pathogens

Nature Nanotechnology, Год журнала: 2023, Номер 18(8), С. 922 - 932

Опубликована: Июнь 1, 2023

Язык: Английский

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Microfluidic systems for infectious disease diagnostics

Lab on a Chip, Год журнала: 2024, Номер 24(5), С. 1441 - 1493

Опубликована: Янв. 1, 2024

This review explores emerging microfluidic-based technologies incorporating innovative assay strategies for infectious disease diagnostics. Our classification scheme is based on the human body systems or pathogen transmission modes.

Язык: Английский

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Multi-Template Molecularly Imprinted Polymeric Electrochemical Biosensors

Chemosensors, Год журнала: 2025, Номер 13(1), С. 11 - 11

Опубликована: Янв. 8, 2025

Dual- or multi-template molecularly imprinted polymers have been an attractive research field for many years as they allow simultaneous detection of more than one target with high selectivity and sensitivity by creating template-specific recognition sites multiple targets on the same functional monomer. Dual/multi-template molecular imprinting techniques applied to identify, extract, detect targets, from heavy metal ions viruses, different methods, such high-performance liquid chromatography (HPLC), chromatography–mass spectrometry (LC-MS), piezoelectric, optical, electrochemical methods. This article focuses sensors based dual/multi-template detecting a wide range Furthermore, this work highlights use these point-of-care applications, their commercialization integration microfluidic systems.

Язык: Английский

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Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva

Advanced Science, Год журнала: 2022, Номер 10(6)

Опубликована: Дек. 30, 2022

Abstract The focus on precise medicine enhances the need for timely diagnosis and frequent monitoring of chronic diseases. Moreover, recent pandemic severe acute respiratory syndrome coronavirus 2 poses a great demand rapid detection surveillance viral infections. protein biomarkers antigens in saliva allows identification diseases or disease changes scenarios where when test response at point care is mandated. While traditional methods testing fail to provide desired fast results, electrochemical biosensors based nanomaterials hold perfect characteristics point‐of‐care settings. advances sensors salivary are critically reviewed this work, with emphasis role boost biosensor analytical performance increase reliability human samples. Furthermore, work identifies critical factors further modernization nanomaterial‐based sensors, envisaging development implementation next‐generation sample‐in‐answer‐out systems.

Язык: Английский

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MoS₂-Plasmonic Nanocavities for Raman Spectra of Single Extracellular Vesicles Reveal Molecular Progression in Glioblastoma

ACS Nano, Год журнала: 2023, Номер 17(13), С. 12052 - 12071

Опубликована: Июнь 27, 2023

Extracellular vesicles (EVs) are continually released from cancer cells into biofluids, carrying actionable molecular fingerprints of the underlying disease with considerable diagnostic and therapeutic potential. The scarcity, heterogeneity intrinsic complexity tumor EVs present a major technological challenge in real-time monitoring complex cancers such as glioblastoma (GBM). Surface-enhanced Raman spectroscopy (SERS) outputs label-free spectroscopic fingerprint for EV profiling. However, it has not been exploited to detect known biomarkers at single level. We developed multiplex fluidic device embedded arrayed nanocavity microchips (MoSERS microchip) that achieves 97% confinement minute amount fluid (<10 μL) enables profiling SERS. arrays combine two featuring characteristics: (1) An MoS2 monolayer isolation nanoconfinement due physical interaction (Coulomb van der Waals) between edge sites lipid bilayer; (2) A layered plasmonic cavity sufficient electromagnetic field enhancement inside cavities obtain level signal resolution stratifying alterations. used GBM paradigm demonstrate potential SERS approach. MoSERS multiplexing parallel acquisition glioma variants (EGFRvIII oncogenic mutation MGMT expression) cells. detection limit 1.23% was found these key wild-type population. When interfaced convolutional neural network (CNN), improved accuracy (87%) which mutations were detected 12 patient blood samples, on par clinical pathology tests. Thus, demonstrates stratification patients using circulating EVs.

Язык: Английский

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Application of microfluidic technologies on COVID-19 diagnosis and drug discovery

Acta Pharmaceutica Sinica B, Год журнала: 2023, Номер 13(7), С. 2877 - 2896

Опубликована: Фев. 24, 2023

The ongoing coronavirus disease 2019 (COVID-19) pandemic has boosted the development of antiviral research. Microfluidic technologies offer powerful platforms for diagnosis and drug discovery severe acute respiratory syndrome 2 (SARS-CoV-2) discovery. In this review, we introduce structure SARS-CoV-2 basic knowledge microfluidic design. We discuss application devices in based on detecting viral nucleic acid, antibodies, antigens. highlight contribution lab-on-a-chip to manufacturing point-of-care equipment accurate, sensitive, low-cost, user-friendly virus-detection devices. then investigate efforts organ-on-a-chip lipid nanoparticles (LNPs) synthesizing chips screening mRNA vaccine preparation. contribute research provide tools future outbreaks.

Язык: Английский

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Photoelectrochemical sensing of titanium oxide nanostructures for the detection of glucose: Fabrication methods and signal enhancement strategies

Microchemical Journal, Год журнала: 2024, Номер 201, С. 110528 - 110528

Опубликована: Апрель 16, 2024

Язык: Английский

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Molecularly Imprinted Polymer Biosensor Based on Nitrogen-Doped Electrochemically Exfoliated Graphene/Ti₃ CNT_X MXene Nanocomposite for Metabolites Detection

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(21), С. 27714 - 27727

Опубликована: Май 8, 2024

Rapid and accurate quantification of metabolites in different bodily fluids is crucial for a precise health evaluation. However, conventional metabolite sensing methods, confined to centralized laboratory settings, suffer from time-consuming processes, complex procedures, costly instrumentation. Introducing the MXene/nitrogen-doped electrochemically exfoliated graphene (MXene@N-EEG) nanocomposite as novel biosensing platform this work addresses challenges associated with leveraging concept molecularly imprinted polymers (MIP) enables highly sensitive, specific, reliable detection metabolites. To validate our technology, we utilize agmatine significant biologically active metabolite. The MIP biosensor incorporates electrodeposited Prussian blue nanoparticles redox probe, facilitating direct electrical signaling binding polymeric matrix. MXene@N-EEG nanocomposite, excellent metal conductivity large electroactive specific surface area, effectively stabilizes nanoparticles. Furthermore, increasing content agmatine-imprinted cavities on electrode enhances sensitivity biosensor. Evaluation designed buffer solution plasma samples reveals wide linear concentration range 1.0 nM–100.0 μM (R2 = 0.9934) limit 0.1 nM. Notably, developed microfluidic offers low cost, rapid response time target molecule (10 min sample incubation), good recovery results detecting samples, acceptable autonomous performance on-chip detection. Moreover, its high reliability position MIP-based promising candidate miniaturized devices potential scalable production point-of-care applications.

Язык: Английский

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Microfluidic immunoassays for point-of-care testing of SARS-CoV-2 antigens and antibodies

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 177, С. 117809 - 117809

Опубликована: Июнь 6, 2024

Язык: Английский

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A Versatile Biomimic Nanotemplating Fluidic Assay for Multiplex Quantitative Monitoring of Viral Respiratory Infections and Immune Responses in Saliva and Blood

Advanced Science, Год журнала: 2022, Номер 9(33)

Опубликована: Окт. 17, 2022

Abstract The last pandemic exposed critical gaps in monitoring and mitigating the spread of viral respiratory infections at point‐of‐need. A cost‐effective multiplexed fluidic device (NFluidEX), as a home‐test kit analogous to glucometer, that uses saliva blood for parallel quantitative detection infection body's immune response an automated manner within 11 min is proposed. technology integrates versatile biomimetic receptor based on molecularly imprinted polymers core–shell structure with nano gold electrodes, fluidic‐impedimetric readout, built‐in collection/preparation, smartphone‐enabled data acquisition interpretation. NFluidEX validated Influenza H1N1 SARS‐CoV‐2 (original strain variants concern), achieves low limit proteins anti‐receptor binding domain (RBD) Immunoglobulin G (IgG) M (IgM), respectively. It demonstrated nanoprotrusions electrodes are essential fine templating antibodies spike during molecular imprinting, differentiation IgG IgM whole blood. In clinical setting, 100% sensitivity specificity by testing 44 COVID‐positive 25 COVID‐negative samples par real‐time polymerase chain reaction ( p < 0.001, 95% confidence) enzyme‐linked immunosorbent assay.

Язык: Английский

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