Human Cell, Год журнала: 2024, Номер 37(4), С. 904 - 916
Опубликована: Май 14, 2024
Язык: Английский
Pathology - Research and Practice, Год журнала: 2024, Номер 260, С. 155354 - 155354
Опубликована: Май 15, 2024
Язык: Английский
Процитировано
36
Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)
Опубликована: Май 15, 2024
Mesenchymal stem/stromal cells (MSCs) represent a heterogeneous cell population distributed throughout various tissues, demonstrating remarkable adaptability to microenvironmental cues and holding immense promise for disease treatment. However, the inherent diversity within MSCs often leads variability in therapeutic outcomes, posing challenges clinical applications. To address this heterogeneity, purification of MSC subpopulations through marker-based isolation has emerged as promising approach ensure consistent efficacy. In review, we discussed reported markers MSCs, encompassing those developed candidate marker strategies high-throughput approaches, with aim explore viable addressing heterogeneity illuminate prospective research directions field.
Язык: Английский
Процитировано
20
Advanced Science, Год журнала: 2022, Номер 10(4)
Опубликована: Дек. 11, 2022
Abstract The highly heterogeneous characteristics of Wharton's jelly mesenchymal stem cells (WJ‐MSCs) may be responsible for the poor clinical outcomes and reproducibility treatments based on WJ‐MSCs. Exploration WJ‐MSC heterogeneity with multimodal single‐cell technologies will aid in establishing accurate MSC subtyping developing screening protocols dominant functional subpopulations. Here, WJ‐MSCs are systematically analyzed by single cell spatial transcriptome sequencing. Single‐cell transcriptomics analysis identifies four subpopulations, namely proliferative_MSCs, niche‐supporting_MSCs, metabolism‐related_MSCs biofunctional‐type_MSCs. Furthermore, transcriptome, cellular heterogeneity, cell‐state trajectories these subpopulations characterized. Intriguingly, biofunctional‐type MSCs (marked S100A9, CD29, CD142) selected this study exhibit promising wound repair properties vitro vivo. Finally, integrating omics data, it has been found that S100A9 + CD29 CD142 subpopulation is more enriched fetal segment umbilical cord, suggesting deriving from have potential into an ideal therapeutic agent healing. Overall, presented comprehensively maps provides essential resource future development WJ‐MSC‐based drugs.
Язык: Английский
Процитировано
45
Bioactive Materials, Год журнала: 2024, Номер 39, С. 492 - 520
Опубликована: Май 30, 2024
Endogenous regeneration is becoming an increasingly important strategy for wound healing as it facilitates skin's own regenerative potential self-healing, thereby avoiding the risks of immune rejection and exogenous infection. However, currently applied biomaterials inducing endogenous skin are simplistic in their structure function, lacking ability to accurately mimic intricate tissue regulate disordered microenvironment. Novel biomimetic with precise structure, chemical composition, biophysical properties offer a promising avenue achieving perfect regeneration. Here, we outline recent advances materials induced from aspects structural functional mimicry, physiological process regulation, property design. Furthermore, novel techniques including situ reprograming, flexible electronic skin, artificial intelligence, single-cell sequencing, spatial transcriptomics, which have contribute development highlighted. Finally, prospects challenges further research application discussed. This review provides reference address clinical problems rapid high-quality
Язык: Английский
Процитировано
10
Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11
Опубликована: Июнь 27, 2023
Accumulating evidence indicates that most primary Wharton’s jelly mesenchymal stem cells (WJ-MSCs) therapeutic potential is due to their paracrine activity, i.e., ability modulate microenvironment by releasing bioactive molecules and factors collectively known as secretome. These can either be released directly into the surrounding or embedded within membrane-bound extracellular nano-sized (usually 30–150 nm) messenger particles vesicles of endosomal origin with specific route biogenesis, exosomes carried relatively larger (100 nm–1 μm) formed outward blebbing plasma membrane called microvesicles (MVs); MVs are (EVs). The found in secretome various types, including cytokines, chemokines, cytoskeletal proteins, integrins, growth factors, angiogenic mediators, hormones, metabolites, regulatory nucleic acid molecules. As expected, performs different biological functions, such immunomodulation, tissue replenishment, cellular homeostasis, besides possessing anti-inflammatory anti-fibrotic effects. This review highlights current advances research on WJ-MSCs’ its prospective clinical applications.
Язык: Английский
Процитировано
23
APOPTOSIS, Год журнала: 2023, Номер 29(1-2), С. 103 - 120
Опубликована: Окт. 24, 2023
Язык: Английский
Процитировано
19
Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 3307 - 3334
Опубликована: Май 1, 2024
Abstract: Inflammatory bowel disease (IBD) is a chronic inflammatory of the gastrointestinal tract, which has high recurrence rate and incurable due to lack effective treatment. Mesenchymal stromal cells (MSCs) are class pluripotent stem that have recently received lot attention their strong self-renewal ability immunomodulatory effects, large number experimental clinical models confirmed positive therapeutic effect MSCs on IBD. In preclinical studies, MSC treatment for IBD relies paracrine cell-to-cell contact, its mediated mitochondrial transfer immune regulation. It also plays role in restoring intestinal mucosal barrier through homing effect, regulation microbiome, repair epithelial cells. latest trials, safety efficacy been by transfusion autologous or allogeneic bone marrow, umbilical cord, adipose MSCs, as well derived extracellular vesicles. However, regarding stable use several concerns emerge, including cell sources, management (dose, route frequency administration, pretreatment MSCs) adverse reactions. This article comprehensively summarizes effects mechanisms advantages over conventional drugs, trial progress The current challenges future directions discussed. review would add knowledge into understanding applying MSCs. Keywords: mesenchymal cells, immunomodulation, disease, ulcerative colitis, Crohn's therapy
Язык: Английский
Процитировано
9
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Апрель 12, 2024
Abstract The current first-line treatment for repairing cartilage defects in clinical practice is the creation of microfractures (MF) to stimulate release mesenchymal stem cells (MSCs); however, this method has many limitations. Recent studies have found that MSC-derived extracellular vesicles (MSC-EVs) play an important role tissue regeneration. This study aimed verify whether MSC-EVs promote damage repair mediated by MFs and explore mechanisms. In vitro experiments showed human umbilical cord Wharton’s jelly (hWJMSC-EVs) promoted vitality chondrocytes proliferation differentiation ability bone marrow-derived MSCs. was mainly because hWJMSC-EVs carry integrin beta-1 (ITGB1), MSCs overexpress ITGB1 after absorbing EVs, thereby activating transforming growth factor-β/Smad2/3 axis. a rabbit knee joint model osteochondral defect repair, injection different concentrations into cavity concentration 50 µg/ml significantly improved formation transparent MF surgery. Extraction regenerated revealed changes ITGB1, factor-β, Smad2/3 were directly proportional cartilage. summary, Graphical abstract
Язык: Английский
Процитировано
8
Biomedicines, Год журнала: 2024, Номер 12(3), С. 683 - 683
Опубликована: Март 19, 2024
Spinal cord injury (SCI), a prevalent and disabling neurological condition, prompts growing interest in stem cell therapy as promising avenue for treatment. Dental-derived cells, including dental pulp cells (DPSCs), from human exfoliated deciduous teeth (SHED), the apical papilla (SCAP), follicle (DFSCs), are of due to their accessibility, minimally invasive extraction, robust differentiating capabilities. Research indicates potential differentiate into neural promote SCI repair animal models at both tissue functional levels. This review explores applications dental-derived repair, covering transplantation, conditioned culture medium injection, bioengineered delivery systems, exosomes, extracellular vesicle treatments, combined therapies. Assessing clinical effectiveness treatment SCI, further research is necessary. includes investigating biological mechanisms conducting Large-animal studies trials. It also important undertake more comprehensive comparisons, optimize selection types, implement functionalized system. These efforts will enhance therapeutic repairing SCI.
Язык: Английский
Процитировано
7
Life Sciences, Год журнала: 2024, Номер 357, С. 123066 - 123066
Опубликована: Сен. 19, 2024
Язык: Английский
Процитировано
7