H₂S‐Releasing Versatile Montmorillonite Nanoformulation Trilogically Renovates the Gut Microenvironment for Inflammatory Bowel Disease Modulation

Advanced Science, Год журнала: 2024, Номер 11(14)

Опубликована: Фев. 2, 2024

Abstract Abnormal activation of the intestinal mucosal immune system, resulting from damage to barrier and extensive invasion by pathogens, contributes pathogenesis inflammatory bowel disease (IBD). Current first‐line treatments for IBD have limited efficacy significant side effects. An innovative H 2 S‐releasing montmorillonite nanoformulation (DPs@MMT) capable remodeling homeostasis, repairing barrier, modulating gut microbiota is developed electrostatically adsorbing diallyl trisulfide‐loaded peptide dendrimer nanogels (DATS@PDNs, abbreviated as DPs) onto (MMT) surface. Upon rectal administration, DPs@MMT specifically binds covers damaged mucosa, promoting accumulation subsequent internalization DPs activated cells in site. release S intracellularly response glutathione, initiating multiple therapeutic In vitro vivo studies shown that effectively alleviates colitis eliminating reactive oxygen species (ROS), inhibiting inflammation, eradicating pathogens. RNA sequencing revealed exerts immunoregulatory repair effects, activating pathways such Nrf2/HO‐1, PI3K‐AKT, RAS/MAPK/AP‐1, p38/ERK MAPK, p65 NF‐κB, JAK‐STAT3 pathways, well glycolysis. 16S rRNA demonstrated remodels pathogens increasing probiotics. This study develops a promising management.

Язык: Английский

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Synergistic Effects of NO/H2S Gases on Antibacterial, Anti-inflammatory, and Analgesic Properties in Oral Ulcers Using a Gas-Releasing Nanoplatform

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Aminopeptidase N‐Activated Self‐immolative Hydrogen Sulfide Donor for Inflammatory Response‐Specific Wound Healing

Angewandte Chemie International Edition, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Abstract Hydrogen sulfide (H 2 S) plays crucial inflammatory modulating roles, representing a promising candidate for anti‐inflammatory therapies. However, current H S delivery approaches lack sufficient specificity against response. Herein, regarding the overexpressed aminopeptidase N (APN) at inflammation sites, an APN‐activated self‐immolative carbonyl (COS)/H donor ( AlaCOS ) was developed response‐specific delivery. The compound showed sustained generation upon APN activation in presence of carbonic anhydrase (CA), and responsiveness could be well regulated by amino acid sequence. Due to delivery, provided potent capability, which further validated RNA sequencing. In vivo experiments on full‐thickness cutaneous wound murine model also strong promoting effect healing, mainly due regulation response . By introducing caged coumarin fluorophore molecular architecture, self‐reporting fluorescence generated accompanied with APN‐mediated COS/H release, achieved visualization vitro vivo. This work not only offers useful tool studying bioactivity inflammation, but provides new insights developing novel therapies cope inflammation‐associated diseases.

Язык: Английский

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Ternary inulin hydrogel with long-term intestinal retention for simultaneously reversing IBD and its fibrotic complication

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 28, 2024

Excessive accumulation of reactive oxygen and nitrogen species (RONS) dysbiosis intestinal microbiota are pivotal symptoms for inflammatory bowel disease (IBD) its associated complications, such as fibrosis. This research introduces a probiotic inulin hydrogel loaded with polypyrrole (PPy) nanozymes antifibrotic drug pirfenidone (PFD) (PPy/PFD@Inulin gel) designed the concurrent amelioration IBD fibrotic complication. Upon oral administration, gel matrix could extend gastrointestinal residence time PPy PFD, facilitating efficient reduction pro-inflammatory cytokine levels enhancement epithelial barrier repair well suppression fibrosis through sustained RONS scavenging, modulation gut attenuation TGF-β/Smad signaling pathway to inhibit fibroblast proliferation. Notably, PPy/PFD@Inulin demonstrated significant prophylactic therapeutic efficacy in acute chronic colitis induced by dextran sodium sulfate (DSS) mouse models. Thus, engineered ternary offered pioneered paradigm simultaneous reversal fibrosis, single regimen.

Язык: Английский

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Microenvironment‐Activatable Probe for Precise NIR‐II Monitoring and Synergistic Immunotherapy in Rheumatoid Arthritis

Advanced Materials, Год журнала: 2024, Номер 36(48)

Опубликована: Окт. 6, 2024

Rheumatoid arthritis (RA) represents an insidious autoimmune inflammatory disorder that severely lowers the life quality by progressively destructing joint functions and eventually causing permanent disability, posing a serious public health problem. Here, advanced theranostic probe is introduced integrates activatable second near-infrared (NIR-II) fluorescence imaging for precise RA diagnosis with multi-pronged treatments. A novel molecular comprising long-wavelength aggregation-induced emission unit manganese carbonyl cage motif synthesized, which enables NIR-II activation concurrently releasing therapeutic carbon monoxide (CO) gas in inflamed microenvironment. This self-assembles into biocompatible nanoprobe, subsequently conjugated anti-IL-6R antibody to afford active-targeting ability of RA. The nanoprobe exhibits significant turn-on signal at lesion, enabling highly sensitive real-time monitoring. combination ROS scavenging, on-demand CO release, IL-6 signaling blockade results potent effect synergistic immunomodulation impact, significantly alleviating symptoms preventing destruction. research introduces paradigm development high-performance, strategies facilitate detection enhanced treatment RA-related diseases.

Язык: Английский

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Protective effect of carbon dots as antioxidants on intestinal inflammation by regulating oxidative stress and gut microbiota in nematodes and mouse models

International Immunopharmacology, Год журнала: 2024, Номер 131, С. 111871 - 111871

Опубликована: Март 16, 2024

Язык: Английский

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Multifunctional injectable hydrogel with self-supplied H2S release and bacterial inhibition for the wound healing with enhanced macrophages polarization via interfering with PI3K/Akt pathway

Biomaterials, Год журнала: 2025, Номер 318, С. 123144 - 123144

Опубликована: Янв. 25, 2025

Язык: Английский

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Exploring effects of gut microbiota on tertiary lymphoid structure formation for tumor immunotherapy

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Март 7, 2025

Anti-tumor immunity, including innate and adaptive immunity is critical in inhibiting tumorigenesis development of tumor. The needs specific lymph organs such as tertiary lymphoid structures (TLSs), which are highly correlated with improved survival outcomes many cancers. In recent years, increasing attention on the TLS tumor microenvironment, TLSs have emerged a novel target for anti-tumor therapy. Excitingly, studies shown contribution to immune responses. However, it unclear how form more effectively defense against through formation. Recent that inflammation plays role Interestingly, also found gut microbiota can regulate occurrence inflammation. Therefore, we here summarize potential effects microbiota- mediated or immunosuppression formation environments. Meanwhile, this review explores manipulate mature regulating microbiota/metabolites associated signal pathways potentially lead next-generation cancer immunotherapy.

Язык: Английский

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DNA Nanostructures Treat Inflammatory Bowel Disease through ROS Scavenging and Gut Microbiota Modulation

Advanced Functional Materials, Год журнала: 2024, Номер 34(38)

Опубликована: Май 7, 2024

Abstract Inflammatory bowel disease (IBD) encompasses a collection of chronic inflammatory conditions impacting the gastrointestinal tract, with discernible global rise in prevalence and severity. The overabundance reactive oxygen species (ROS) intestinal environment leads disruption local redox homeostasis, perturbation gut microbiota, exacerbating IBD. Consequently, mitigating excess ROS preserving microbiota homeostasis have emerged as effective approaches for IBD management. In this study, triangular DNA origami nanostructure loaded siRNA targeting tumor necrosis factor α ( siTNF‐α tDON) is introduced purpose. This demonstrates efficacy eliminating within cells closely associated IBD, diminishing inflammation, ameliorating disorders evidenced an mouse model. Furthermore, investigation effects tDON on related signaling pathways, including NF‐κB, MAPK/ERK Keap1/Nrf2. Collectively, study offers insights into potential utilization nanostructures antioxidants modulators, presenting promising avenues treatment.

Язык: Английский

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Glutathione-responsive nanoplatforms trigger gaseous intervention of intestinal inflammation through TLR4/MD2/MyD88/NF-κB/iNOS pathway activation and gut microbiota modulation

Chemical Engineering Journal, Год журнала: 2024, Номер 493, С. 152849 - 152849

Опубликована: Июнь 5, 2024

Язык: Английский

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