Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro, in Both a Normal and Inflammatory Milieu

ACS Nano, Год журнала: 2024, Номер 18(3), С. 2047 - 2065

Опубликована: Янв. 2, 2024

The use of piezoelectric nanomaterials combined with ultrasound stimulation is emerging as a promising approach for wirelessly triggering the regeneration different tissue types. However, it has never been explored boosting chondrogenesis. Furthermore, parameters used are often not adequately controlled. In this study, we show that adipose-tissue-derived mesenchymal stromal cells embedded in nanocomposite hydrogel containing barium titanate nanoparticles and graphene oxide nanoflakes stimulated waves precisely controlled (1 MHz 250 mW/cm2, 5 min once every 2 days 10 days) dramatically boost chondrogenic cell commitment vitro. Moreover, fibrotic catabolic factors strongly down-modulated: proteomic analyses reveal such influences biological processes involved cytoskeleton extracellular matrix organization, collagen fibril metabolic processes. optimal regimen also considerable anti-inflammatory effect keeps its ability to chondrogenesis vitro, even an inflammatory milieu. An analytical model predict voltage generated by invested proposed, together computational tool takes into consideration nanoparticle clustering within vacuoles predicts electric field streamline distribution cytoplasm. proposed shows good injectability adhesion cartilage ex vivo, well excellent biocompatibility according ISO 10993. Future perspectives will involve preclinical testing paradigm regeneration.

Язык: Английский

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A Biodegradable Piezoelectric Sensor for Real‐Time Evaluation of the Motor Function Recovery After Nerve Injury

Advanced Functional Materials, Год журнала: 2024, Номер 34(33)

Опубликована: Март 22, 2024

Abstract Nerve injury can lead to defects in related motor functions. It is critical achieve long‐term and convenient real‐time evaluation of function recovery status during nerve repair. In this study, an implantable PLLA/BTO piezoelectric sensor (PBPS) with good biodegradability biocompatibility for real time the after developed. PLLA fibers doped BTO are employed as material PBPS, which convert biomechanical signals generated by motion into electrical signals. PBPS be implant simultaneously commonly used tissue scaffolds treatment rats sciatic injury. The linearity pressure output voltage ≈0.9445. For effectiveness, process progresses, exhibited consistency EMG signals, indicating effectively function. Moreover, integration wireless module break limitations space sensing realize rat. based on may bring new ideas development bioelectronics.

Язык: Английский

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Degradable piezoelectric biomaterials for medical applications

MedMat., Год журнала: 2024, Номер 1(1), С. 40 - 49

Опубликована: Май 13, 2024

The energy harvesting technology based on piezoelectricity promises to achieve a self-powered mode for portable medical electronic devices. Piezoelectric materials, as crucial components in electromechanical applications, have extensively been utilized Especially, degradable piezoelectric biomaterials received much attention the field due their excellent biocompatibility and biosafety. This mini-review mainly summarizes types structural characteristics of from small-molecule crystals polymers. Afterward, applications are briefly introduced, including harvester sensor, actuator transducer, tissue engineering scaffold. Finally, material perspective, some challenges currently faced by proposed.

Язык: Английский

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Advancements in Metal and Metal Oxide Nanoparticles for Targeted Cancer Therapy and Imaging: Mechanisms, Applications, and Safety Concerns

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106622 - 106622

Опубликована: Янв. 1, 2025

Язык: Английский

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Advances in Electrical Materials for Bone and Cartilage Regeneration: Developments, Challenges, and Perspectives

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Abstract Severe bone and cartilage defects caused by trauma are challenging to treat, often resulting in poor outcomes. An endogenous electric field (EnEF) is crucial for regeneration, making electrical materials a promising therapy. This review provides comprehensive overview of the role bioelectric signals cells, alongside recent advancements biomaterials, with particular emphasis on nanogenerators, piezoelectric materials, triboelectric scaffolds, zwitterionic hydrogels. It further investigates impact these biomaterials as well applications both exogenous stimulation (ES) mechanisms underlying ES‐induced cellular molecular responses. Finally, underscores future directions ES systems tissue engineering, emphasizing critical importance integrating structural integrity, mechanical properties, signal delivery into intelligent implantable scaffolds.

Язык: Английский

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Piezoelectric PVDF Nanoparticles for Enhanced Antimicrobial Activity via Mechanical Stimulation: A Proof-of-Concept Study

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

The alarming rise of antimicrobial resistance is a public health issue, driven by the excessive and improper use antibiotics, which are becoming less effective against an increasing number microorganisms. There urgent need to find alternative strategies that can bypass bacterial mechanisms. Using physical stimuli sensitize bacteria action one step toward addressing this challenge. In work, piezoelectric poly(vinylidene fluoride) (PVDF) nanoparticles were developed in attempt control enhance activity materials through stimulation. exhibited sizes ranging from 200 400 nm, with low polydispersity, negative surface charge, spherical smooth morphology. reprecipitation methodology, synthesized crystallization PVDF electroactive β-phase, achieving percentages formulations greater than 80%. These demonstrated promising properties, considerably enhanced dynamic conditions involving mechanical stimulation resulting creation microenvironments. Notably, approach stronger inhibitory effect on growth, particularly Escherichia coli. When water was used as nonsolvent for concentration 10 mg/mL, it resulted inhibition, reductions 1.33 log10 under static 2.21 conditions. However, pronounced Staphylococcus aureus. contrast, when 50% ethanol solution nonsolvent, both significant reductions: E. coli completely eradicated conditions, while S. aureus showed 1.93 reduction. Under eliminated. Although these compromise viability human fibroblasts after 72 h contact, study provides proof-of-concept findings open possibilities developing hygienic coatings surfaces, leveraging pressure or touch activate antibacterial effects.

Язык: Английский

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Piezoelectric Nanomaterials Activated by Ultrasound in Disease Treatment

Pharmaceutics, Год журнала: 2023, Номер 15(5), С. 1338 - 1338

Опубликована: Апрель 26, 2023

Electric stimulation has been used in changing the morphology, status, membrane permeability, and life cycle of cells to treat certain diseases such as trauma, degenerative disease, tumor, infection. To minimize side effects invasive electric stimulation, recent studies attempt apply ultrasound control piezoelectric effect nano material. This method not only generates an field but also utilizes benefits non-invasive mechanical effects. In this review, important elements system, piezoelectricity nanomaterial ultrasound, are first analyzed. Then, we summarize categorized into five kinds, nervous system treatment, musculoskeletal tissues cancer anti-bacteria therapy, others, prove two main mechanics under activated piezoelectricity: one is biological change on a cellular level, other piezo-chemical reaction. However, there still technical problems be solved regulation processes completed before widespread use. The core include how accurately measure properties, concisely electricity release through complex energy transfer processes, deeper understanding related bioeffects. If these conquered future, nanomaterials by will provide new pathway realize application disease treatment.

Язык: Английский

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The potential application of electrical stimulation in tendon repair: a review

Med-X, Год журнала: 2025, Номер 3(1)

Опубликована: Март 4, 2025

Язык: Английский

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Tumor microenvironment-responsive self-assembly of barium titanate nanoparticles with enhanced piezoelectric catalysis capabilities for efficient tumor therapy

Bioactive Materials, Год журнала: 2023, Номер 33, С. 251 - 261

Опубликована: Ноя. 20, 2023

Catalytic therapy based on piezoelectric nanoparticles has become one of the effective strategies to eliminate tumors. However, it is still a challenge improve tumor delivery efficiency nanoparticles, so that they can penetrate normal tissues while specifically aggregating at sites and subsequently generating large amounts reactive oxygen species (ROS) achieve precise efficient clearance. In present study, we successfully fabricated microenvironment-responsive assembled barium titanate (tma-BTO NPs): in neutral pH environment tissues, tma-BTO NPs were monodisperse possessed ability cross intercellular space; whereas, acidic triggered self-assembly form submicron-scale aggregates, deposited microenvironment. The self-assembled not only caused mechanical damage cells; more interestingly, also exhibited enhanced catalytic produced ROS than under ultrasonic excitation, attributed mutual extrusion neighboring particles within confined space assembly. differential cytotoxicity against cells cells, stronger catalysis induced by assemblies resulted significant apoptosis mouse breast cancer (4T1); there was little embryo osteoblast precursor (MC3T3-E1) same treatment conditions. Animal experiments confirmed peritumoral injection combined with ultrasound effectively inhibit progression non-invasively. strategy opens up new perspectives for future piezoelectric-catalyzed therapy.

Язык: Английский

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Piezoelectric PVDF and its copolymers in biomedicine: innovations and applications

Biomaterials Science, Год журнала: 2024, Номер 12(20), С. 5164 - 5185

Опубликована: Янв. 1, 2024

In recent years, poly(vinylidene fluoride) (PVDF) has emerged as a versatile polymer with wide range of applications across various fields. PVDF's piezosensitivity, versatility, crystalline structure, and tunable parameters have established it highly sought-after material. Furthermore, PVDF its copolymers exhibit excellent processability chemical resistance to diverse array substances. Of particular significance is remarkable structural stability in physiological media, which highlights potential for use the development biomedical products. This review offers comprehensive overview latest advancements PVDF-based systems. It examines fabrication stimulus-responsive delivery systems, bioelectric therapy devices, tissue-regenerating scaffolds, all harness piezosensitivity PVDF. Moreover, both invasive non-invasive diagnostic tools investigated, emphasis on flexibility, transparency, piezoelectric efficiency. The material's high biocompatibility are paramount importance implantable sensors long-term health monitoring, crucial management chronic diseases postoperative care. Additionally, we discuss novel approach photoacoustic microscopy that employs sensor, thereby eliminating necessity external contrast agents. technique provides new avenue imaging applications. Finally, explore challenges prospects systems distinctive comparison other reviews due concentrated examination applications, including pioneering techniques, detailed account field. Collectively, these elements illustrate markedly influence engineering patient care, distinguishing from existing literature. By leveraging attributes copolymers, researchers can continue advance frontiers engineering, transform care treatment outcomes.

Язык: Английский

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