On‐Site Non‐enzymatic Orthogonal Activation of a Catalytic DNA Circuit for Self‐Reinforced In Vivo MicroRNA Imaging

Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(45)

Опубликована: Июль 1, 2022

Abstract The wide extracellular‐intracellular distribution of microRNA requires the on‐site, robust and efficient activation catalytic DNA circuits inside live cells. Herein, we develop an non‐enzymatic circuitry strategy to realize orthogonally controlled (CCD) circuit for achieving high‐fidelity in vivo imaging through multiply guaranteed molecular recognition progressively accelerated signal amplification. For predictable on‐site useful efficiency, dominating fuel strand was initially split into inactive subunits that were grafted auxiliary circuit. There, in‐cell‐specific mRNA triggered orthogonal amplification active strands sensitive target detection chief entropy‐driven We believe method can contribute clinical diagnosis prognosis.

Язык: Английский

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Exogenously and Endogenously Sequential Regulation of DNA Nanodevices Enables Organelle‐Specific Signal Amplification in Subcellular ATP Profiling

Angewandte Chemie International Edition, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Abstract Adenosine triphosphate (ATP), the primary energy currency in cells, is dynamically regulated across different subcellular compartments. The ATP interplay between mitochondria and endoplasmic reticulum (ER) underscores their coordinated roles various biochemical processes, highlighting necessity for precise profiling of dynamics. Here we present an exogenously endogenously dual‐regulated DNA nanodevice spatiotemporally selective, subcellular‐compartment specific signal amplification sensing. system allows exogenous NIR light‐controlled spatiotemporal localization activation aptamer sensor or ER, while a endogenous enzyme organelles further drives via consumption molecular beacon fuels, resulting significantly enhanced sensitivity spatial precision organelle interest. Furthermore, demonstrate application this robust monitoring fluctuations ER following drug interventions. This advancement provides powerful tool improving our understanding cellular energetics at level holds potential development targeted therapeutics.

Язык: Английский

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Spatiotemporally Selective Molecular Imaging via Upconversion Luminescence‐Controlled, DNA‐Based Biosensor Technology

Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(32)

Опубликована: Июнь 6, 2022

DNA-based biosensor technologies have shown great potential in chemical and biological detection. These biosensors been actively developed as probes for molecular imaging live cells animals, allowing situ detection of analytes complex systems, elucidation the roles key molecules processes, development non-invasive diagnosis image-guided surgery. Despite progress made, improving spatial-temporal precision remains a challenge this field. In Minireview, we describe concepts behind spatiotemporally selective via combination engineered, light-activatable upconversion nanotechnology. We then highlight application approach controlled various targets specific intracellular organelles, signal amplification, well regulation targeting activity to receptor proteins. finally discuss challenges perspectives possible future developments emerging

Язык: Английский

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Enzymatically Activated Autonomous-Motion DNAzyme Signal Amplification Strategy for Tumor Cell-Specific Molecular Imaging with Improved Spatial Specificity

Analytical Chemistry, Год журнала: 2023, Номер 95(24), С. 9388 - 9395

Опубликована: Июнь 6, 2023

Strategies for achieving tumor-specific molecular imaging based on signal amplification hold great potential evaluating the risk of tumor metastasis and progression. However, traditional strategies are still constrained with limited specificity because off-tumor leakage. Herein, an endogenous enzyme-activated autonomous-motion DNAzyme strategy (E-DNAzyme) was rationally designed improved spatial specificity. The sensing function E-DNAzyme can be specifically activated by overexpressed apurinic/apyrimidinic endonuclease 1 (APE1) in cytoplasm cells instead normal cells, ensuring cell-specific Of note, benefiting from target analogue-triggered autonomous motion strategy, detection limit decreased approx. ∼7.8 times. Moreover, discrimination ratio tumor/normal proposed ∼3.44-fold higher than indicating prospect this universal design imaging.

Язык: Английский

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DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(36)

Опубликована: Июнь 14, 2024

Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio-applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria-targeted tumor treatment. We first highlight engineering mitochondria-localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti-tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Язык: Английский

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Optically Programmable Plasmon Enhanced Fluorescence-Catalytic Hairpin Assembly Signal Amplification Strategy for Spatiotemporally Precise Imaging

Analytical Chemistry, Год журнала: 2022, Номер 94(13), С. 5399 - 5405

Опубликована: Март 23, 2022

Signal amplification strategies with spatiotemporally high sensitivity can provide more accurate information and hold great promise for improving the accuracy of disease diagnosis. Herein, a 808 nm near-infrared (NIR) light-activated plasmon enhanced fluorescence-catalytic hairpin assembly (PEF-CHA) signal strategy was proposed controllable precise imaging miRNA in vitro vivo ultrasensitivity. The NIR PEF-CHA is constructed through combining up-conversion photocontrol PEF technologies CHA. It worth noting that laser irradiation-induced overheating effect could be effectively alleviated by using Nd3+-sensitized upconversion nanoparticles (UCNPs) to convert light ultraviolet (UV) light, which almost nondestructive cells or tissues. In addition, nonspecific activation as well false positive signals avoided. Moreover, detection limit reduced approximate 38 times thanks strategy. Furthermore, we demonstrate expanded sensitive activatable intratumoral miRNAs living mice, showing feasible prospects biological medical analysis.

Язык: Английский

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Spatially Resolved, Error‐Robust Multiplexed MicroRNA Profiling in Single Living Cells

Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(20)

Опубликована: Фев. 23, 2022

Abstract Simultaneous imaging of multiple microRNAs (miRNAs) in individual living cells is challenging due to the lack spectrally distinct encoded fluorophores and non‐cytotoxic methods. We describe a multiplexed error‐robust combinatorial fluorescent label‐encoding method, termed error‐corrected labels (FluoELs), enabling miRNA with error‐correcting capability. The FluoELs comprise proportional dual for encoding constant quantitative single fluorophore quantification. Both are embedded 260 nm core–shell silica nanoparticles modified molecular beacon detection probes. low cytotoxic could accurately quantify spatially resolve nine breast‐cancer‐related miRNAs evaluate their coordination. enabled single‐cell analysis platform expression profiles mechanisms underlying miRNA‐associated diseases.

Язык: Английский

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Fluorescence-Enhanced Dual-Driven “OR-AND” DNA Logic Platform for Accurate Cell Subtype Identification

Analytical Chemistry, Год журнала: 2023, Номер 95(6), С. 3525 - 3531

Опубликована: Фев. 6, 2023

Developing an endogenous stimuli-responsive and ultrasensitive DNA sensing platform that contains a logic gate biocomputation for precise cell subtype identification holds great potential disease diagnosis prognostic estimation. Herein, fluorescence-enhanced "OR-AND" dual-driven by intracellular apurinic/apyrimidinic endonuclease 1 (APE 1) or strand anchored on membrane protein Mucin (MUC sensitive accurate was rationally designed. The recognition toehold of the traditional activated probe (TP) restrained introducing blocking sequence containing APE cleavable site (AP-site) can be either cleaved replaced Mk-apt, ensuring gated molecular imaging identification. It is worth noting this design effectively avoid missing logical computation caused heterogeneous spatial distribution as single input. In addition, benefit from excellent plasmon-enhanced fluorescence (PEF) ability Au NSTs detection limit decreased nearly 165 times. Based this, not only different kinds MCF-7, HepG2, L02 cells, but also breast cancer subtypes, including malignant metastatic MDA-MB-231, nontumorigenic MCF-10A accurately identified proposed platform, indicating prospect simple universal in screening.

Язык: Английский

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Tuning Near‐Infrared‐to‐Ultraviolet Upconversion in Lanthanide‐Doped Nanoparticles for Biomedical Applications

Advanced Optical Materials, Год журнала: 2022, Номер 11(11)

Опубликована: Окт. 25, 2022

Abstract Ultraviolet (UV) radiation offers many exciting biomedical applications such as biosensing and cancer therapy. Photon upconversion through lanthanide (Ln)‐doped nanoparticles is an attractive approach for UV generation by excitation of near‐infrared (NIR) light, which readily accessible highly penetrating in tissue environments. By rationally engineering the emission property (UCNPs) coupled with surface functionalization photoactivatable molecules, various nanosystems can be constructed biomedicine feature minimal living damage remote light delivery at high spatiotemporal precisions. Such technology facilitates sensitive diagnosis effective treatment diseases. This review focuses on recent development NIR‐to‐UV UCNPs, simultaneously highlighting emergent enabled these materials.

Язык: Английский

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Series or parallel toehold-mediated strand displacement and its application in circular RNA detection and logic gates

Biosensors and Bioelectronics, Год журнала: 2023, Номер 241, С. 115677 - 115677

Опубликована: Сен. 7, 2023

Язык: Английский

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