Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA

Angewandte Chemie International Edition, Год журнала: 2020, Номер 59(23), С. 8998 - 9003

Опубликована: Март 11, 2020

We developed a new method for the conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos using photochemically activated, caged guide RNAs (gRNAs). Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout 5'-protospacer region with during synthesis. Caging confers complete suppression gRNA:dsDNA-target hybridization rapid restoration function upon optical activation. This tool offers simplicity programmability design, high spatiotemporal specificity embryos, excellent off-to-on switching, stability preserving ability to form Cas9:gRNA ribonucleoprotein complexes. novel tools control gene editing, thereby enabling investigation spatiotemporally complex physiological events obtaining better understanding dynamic regulation.

Язык: Английский

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Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP‐Tag Technologies

ChemBioChem, Год журнала: 2020, Номер 21(14), С. 1935 - 1946

Опубликована: Март 17, 2020

Visualizing and manipulating the behavior of proteins is crucial to understanding physiology cell. Methods biorthogonal protein labeling are important tools attain this goal. In review, we discuss advances in probe technology specific for self-labeling tags, focusing mainly on application HaloTag SNAP-tag systems. We describe latest developments small-molecule probes that enable fluorogenic (no wash) imaging super-resolution fluorescence microscopy. addition, cover several methodologies perturbation or manipulation function towards control biological pathways. Thus, current technical systems means they becoming powerful visualization processes, providing invaluable scientific insights difficult obtain by traditional methodologies. As multiplex tag continues be developed expanded, utility these tags will allow researchers address previously inaccessible questions at forefront biology.

Язык: Английский

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Development of photolabile protecting groups and their application to the optochemical control of cell signaling

Current Opinion in Structural Biology, Год журнала: 2019, Номер 57, С. 164 - 175

Опубликована: Май 25, 2019

Язык: Английский

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Photoswitchable peptides for spatiotemporal control of biological functions

Chemical Communications, Год журнала: 2019, Номер 55(69), С. 10192 - 10213

Опубликована: Янв. 1, 2019

Light is unsurpassed in its ability to modulate biological interactions. Since their discovery, chemists have been fascinated by photosensitive molecules capable of switching between isomeric forms, known as photoswitches. Photoswitchable peptides recognized for many years; however, functional implementation systems has only recently achieved. Peptides are now acknowledged excellent protein-protein interaction modulators and important the emergence photopharmacology. In this review, we briefly explain different classes photoswitches summarize structural studies when they incorporated into peptides. Importantly, provide a detailed overview rapidly increasing number examples, where modulation driven changes. Furthermore, discuss some remaining challenges faced field. These exciting proof-of-principle highlight tremendous potential photocontrollable optochemical tools chemical biology biomedicine.

Язык: Английский

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A Platinum(II) Complex of Heptamethine Cyanine for Photoenhanced Cytotoxicity and Cellular Imaging in Near‐IR Light

Angewandte Chemie International Edition, Год журнала: 2018, Номер 57(32), С. 10263 - 10267

Опубликована: Июнь 25, 2018

Controlled generation of cytotoxic agents with near-IR light is a current focus photoactivated cancer therapy, including that involving platinum species. A heptamethine cyanine scaffolded Pt

Язык: Английский

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