Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Short-peptide
amyloid
assembly
and
disassembly
play
crucial
roles
in
various
research
fields,
which
range
from
addressing
pathologies
that
lack
therapeutic
solutions
to
the
development
of
innovative
soft
(bio)materials.
Hydrogels
short
peptides
typically
show
thermo-reversible
gel-to-sol
transition,
whereby
fibrils
disassemble
upon
heating,
re-assemble
cooling
down
room
temperature
(rt).
Despite
ongoing
intense
studies
this
area,
majority
focus
on
peptide-peptide
interaction
neglect
structuring
role
water
peptide
supramolecular
behavior.
This
study
describes
an
unprotected
tetrapeptide
gelator
forms
highly
stable
which,
re-organize
into
plates
persist
rt.
All-atom
molecular
dynamics
(MD)
simulations
experimental
methods
reveal
as
a
key
player
thermodynamics
accompany
irreversible
morphological
advance
our
understanding
structures.
Self-assembling
of
peptides
is
essential
for
a
variety
biological
and
medical
applications.
However,
it
challenging
to
investigate
the
self-assembling
properties
within
complete
sequence
space
due
enormous
quantities.
Here,
demonstrated
that
transformer-based
deep
learning
model
effective
in
predicting
aggregation
propensity
(AP)
peptide
systems,
even
decapeptide
mixed-pentapeptide
systems
with
over
10
trillion
Based
on
predicted
AP
values,
not
only
laws
designing
are
derived,
but
transferability
relation
among
APs
pentapeptides,
decapeptides,
mixed
pentapeptides
also
revealed,
leading
discoveries
by
concatenating
or
mixing,
as
consolidated
experiments.
This
approach
enables
speedy,
accurate,
thorough
search
design
oligopeptides,
advancing
science
inspiring
new
Communications Chemistry,
Год журнала:
2024,
Номер
7(1)
Опубликована: Фев. 29, 2024
Peptide-based
liquid-liquid
phase
separated
domains,
or
coacervates,
are
a
biomaterial
gaining
new
interest
due
to
their
exciting
potential
in
fields
ranging
from
biosensing
drug
delivery.
In
this
study,
we
demonstrate
that
coacervates
provide
simple
and
biocompatible
medium
improve
nucleic
acid
biosensors
through
the
sequestration
of
both
biosensor
target
strands
within
coacervate,
thereby
increasing
local
concentration.
Using
well-established
polyarginine
(R
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(25)
Опубликована: Фев. 12, 2024
Abstract
Aqueous
soft
matter,
including
supramolecular
hydrogels
capable
of
exhibiting
stimuli–responsive
macroscopic
phase
transitions,
has
attracted
increasing
attention
for
the
exploration
functional
materials.
However,
investigation
that
undergo
autonomous
and
multiple
transitions
(e.g.,
gel–sol–gel,
sol–gel–sol)
in
response
to
surrounding
environment
without
repeated
additions
stimuli
remained
largely
unexplored.
In
this
study,
oxidation‐responsive
gel–sol–gel
fabricated
via
self‐assembly
a
simple
fluorenylmethyloxycarbonyl
(Fmoc)‐protected,
benzylated
cysteine
(
Fmoc‐C
Bzl
)
is
presented.
During
evaluation
oxidation
process
,
it
revealed
oxidized
products,
two
diastereomeric
sulfoxides
‐(
R
)‐O
S
),
exhibit
significantly
different
propensities
under
aqueous
conditions.
It
may
be
noteworthy
chirality
sulfoxide
overlooked
not
effectively
used
modulate
supramolecular,
self‐assembled
nanostructures.
The
difference
kinetics
self‐assembly/disassembly
as
well
co‐assembly
will
contribute
transitions.
Nanoscale,
Год журнала:
2024,
Номер
16(20), С. 9887 - 9898
Опубликована: Янв. 1, 2024
Delving
into
the
mechanism
behind
molecular
interactions
at
atomic
level
of
short-sequence
peptides
plays
a
key
role
in
development
nanomaterials
with
specific
structure–property–function
relationships
from
bottom-up
perspective.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(38), С. 14475 - 14483
Опубликована: Сен. 11, 2023
We
investigate
the
interactions
between
C-reactive
protein
(CRP)
and
new
CRP-binding
peptide
materials
using
experimental
(biological
physicochemical)
methods
with
support
of
theoretical
simulations
(computational
modeling
analysis).
Three
specific
peptides
(P2,
P3,
P9)
derived
from
an
M13
bacteriophage
have
been
identified
phage-display
technology.
The
binding
efficiency
exposed
on
phages
toward
CRP
was
demonstrated
via
biological
methods.
Fibers
selected
phages/peptides
interact
differently
due
to
different
compositions
amino
acid
sequences
peptides,
which
confirmed
by
transmission
electron
microscopy.
Numerical
studies
consistently
showed
that
P3
is
best
binder.
A
combination
demonstrates
identifying
binder
can
be
performed
simply,
cheaply,
fast.
Such
approach
has
not
reported
previously
for
screening
a
trend
in
science
where
calculations
replace
or
laborious
techniques.
Finally,
binder─the
peptide─was
used
recognition
silicate-modified
indium
tin
oxide-coated
glass
electrodes.
obtained
electrodes
exhibit
wide
range
operation
(1.0-100
μg
mL-1)
detection
limit
(LOD
=
3σ/S)
0.34
mL-1.
Moreover,
dissociation
constant
Kd
4.2
±
0.144
mL-1
(35
1.2
nM)
evaluated
change
current.
selectivity
electrode
presence
three
interfering
proteins.
These
results
prove
presented
potential
candidate
as
receptor
CRP,
antibodies.
