Abstract
Nanozymes
have
recently
gained
attention
for
their
low
cost
and
high
stability.
However,
unlike
natural
enzymes,
they
often
exhibit
multiple
enzyme‐like
activities,
complicating
use
in
selective
bioassays.
Since
H
2
O
are
common
substrates
these
reactions,
controlling
activation—and
thus
reaction
specificity—is
crucial.
Recent
advances
tuning
the
chemical
state
of
cerium
enabled
control
over
activation
pathways
tunable
peroxidase/haloperoxidase‐like
activities.
In
contrast,
on
an
element
oxidase/laccase
nanozymes
impact
its
activities
remains
unexplored.
Herein,
a
facile
one‐pot
method
is
presented
gram‐scale
synthesis
Fe‐based
with
compositions
Fe
3
4
C
by
adjusting
preparation
temperatures.
The
‐containing
samples
superior
laccase‐like
activity,
while
C‐containing
counterparts
demonstrate
better
oxidase‐like
activity.
This
divergent
behavior
linked
to
surface
species:
abundant
reactive
2+
promotes
activity
via
3+
‐superoxo
formation,
whereas
metallic
facilitates
OH
radical
generation
Controlled
improved
sensitivity
corresponding
biomolecule
detection,
which
should
inform
design
enhanced
specificity.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 7, 2025
Abstract
Designing
single‐atom
catalysts
(SACs)
with
high
density
of
accessible
sites
by
improving
metal
loading
and
utilization
is
a
promising
strategy
to
boost
the
catalytic
activity,
but
remains
challenging.
Herein,
site
(SD)
iron
SAC
(D‐Fe‐N/C)
11.8
wt.%
Fe‐loading
reported.
The
in
situ
scanning
electrochemical
microscopy
technique
attests
that
active
SD
D‐Fe‐N/C
reach
as
1.01
×
10
21
g
−1
79.8%,
respectively.
Therefore,
demonstrates
superior
oxygen
reduction
reaction
(ORR)
activity
terms
half‐wave
potential
0.918
V
turnover
frequency
0.41
e
s
.
excellent
ORR
property
also
demonstrated
liquid
zinc‐air
batteries
(ZABs),
which
exhibit
peak
power
306.1
mW
cm
−2
an
ultra‐long
cycling
stability
over
1200
h.
Moreover,
solid‐state
laminated
ZABs
prepared
presetting
air
flow
layer
show
specific
capacity
818.8
mA
h
,
520
h,
wide
temperature‐adaptive
from
−40
60
°C.
This
work
not
only
offers
possibilities
metal‐loading
for
exploring
efficient
SACs,
provides
strategies
device
structure
design
toward
advanced
ZABs.
Late-stage
diagnosis
is
a
major
contributor
to
cancer
mortality
and
thus
leads
increased
fatality,
making
early
detection
crucial
for
improving
survival
rates.
Circulating
tumor
cells
(CTC),
detectable
before
primary
tumors
become
clinically
apparent,
have
emerged
as
vital
biomarkers
the
identification
of
aggressive
cancers.
Here,
develop
single-atom
nanozyme
integrated
nanoarray
3D
nano-biointerface
ultrasensitive
electrochemical
screening
CTCs
from
hepatocellular
carcinoma.
This
cytosensor
capable
identifying
CTC
at
single-cell
level,
achieving
an
impressive
area
under
curve
0.96
in
receiver
operating
characteristics,
comparable
simulated
multi-indicator
diagnostic
strategies.
strategy
shows
great
potential
non-invasive
carcinoma
promising
be
applied
universally
diagnosis.
Angewandte Chemie International Edition,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Abstract
Nanozymes
with
atomically
dispersed
metal
sites
(ADzymes),
especially
single‐atom
nanozymes,
have
attracted
widespread
attention
in
recent
years
due
to
their
unique
advantages
mimicking
the
active
of
natural
enzymes.
These
nanozymes
not
only
maximize
exposure
catalytic
but
also
possess
superior
activity
performance,
achieving
challenging
reactions.
position
ADzymes
as
highly
promising
candidates
field
sensing
and
biosensing.
This
review
summarizes
classification
properties
ADzymes,
systematically
highlighting
some
typical
regulation
strategies
involving
central
metal,
coordination
environment,
etc.,
achieve
catalytical
activity,
specificity,
multifunctionality.
Then,
we
present
advances
different
fields,
including
colorimetry,
fluorescence,
electrochemistry,
chemiluminescence,
photoelectrochemistry,
electrochemiluminescence.
Taking
advantage
resultant
show
great
potential
goal
sensitivity,
selectivity
accuracy
for
detection
various
targets.
Specifically,
underlying
mechanisms
terms
signal
amplification
were
discussed
detail.
Finally,
current
challenges
perspectives
on
development
advanced
are
discussed.
Dual-atom
nanozymes
(DAzymes)
have
garnered
considerable
attention
as
catalysts
for
reactive
oxygen
species
(ROS)-based
therapies,
effectively
leveraging
ROS
generation
within
the
tumor
microenvironment
(TME).
Herein,
we
introduce
FeMn-NCe
DAzymes,
which
are
meticulously
engineered
enhanced
peroxidase
(POD)-mimetic
activity
and
potent
radiosensitization
to
advance
radioimmunotherapy.
Density
functional
theory
(DFT)
calculations
reveal
that
DAzymes
lower
energy
barrier
increase
substrate
affinity,
enabling
highly
efficient
catalytic
performance.
Within
TME,
these
efficiently
convert
overexpressed
hydrogen
peroxide
(H2O2)
into
hydroxyl
radicals
(•OH),
potentially
activating
cGAS-STING
immune
pathway.
This
POD-mimetic
catalysis
is
further
accelerated
under
X-ray
irradiation,
significantly
enhancing
radiosensitization.
Additionally,
a
uniform
coating
of
ultrasmall
gold
nanoparticles
on
enhances
absorption
cancer
cells.
The
incorporation
STING
agonist
diABZI
onto
induces
long-term
antitumor
immunity,
reprograms
immunosuppressive
suppresses
growth
metastasis
following
single
low-dose
treatment.
work
highlights
valuable
strategy
designing
radiodynamic
immunotherapy.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 23, 2025
Abstract
Reactive
oxygen
species
(ROS)
play
crucial
roles
in
cellular
metabolic
processes
by
acting
as
primary
intracellular
chemical
substrates
and
secondary
messengers
for
signal
modulation.
However,
the
artificial
engineering
of
nanozymes
to
generate
ROS
is
restricted
their
low
catalytic
efficiency,
high
toxicity,
off‐target
consumption.
Herein,
hetero‐trimetallic
atom
catalysts
(TACs)
anchored
on
a
stable
symmetrical
pyramid
structure
are
designed
presence
N
P
surface
ligands
from
cross‐linked
polyphosphazene
interlayer‐coated
MIL‐101(Fe).
The
3D
network
TACs
with
uniform
dispersion
Cu,
Co,
Fe
hetero‐single
atoms
effectively
tailor
active
sites
avoid
metal
sintering,
thereby
providing
sufficient
activity
blooms.
Nanovesicle
membranes
facilitate
accumulation
homologous
targeting,
recognition,
endocytosis,
addressing
potentially
toxicity
defects.
Therefore,
outcome
situ
ROS‐bloom
acts
redox
directly
regulating
oxidative
stress
tumor
microenvironment.
Meanwhile,
intervene
glutathione
peroxidase
4,
long‐chain
acyl‐CoA
synthetase
cysteinyl
aspartate
specific
proteinase‐3
pathways
second
messengers,
fostering
proclivity
toward
apoptosis
lipid
peroxidation‐regulated
ferroptosis
pathway
concurrently,
highlighting
application
prospects
biomedical
field.
Understanding
electronic
effects
on
catalysis
from
a
mechanism
point
of
view
is
fundamental
significance
but
also
challenging.
We
herein
report
the
oxygen
reduction
reaction
(ORR)
with
Fe
porphyrins.
By
using
FeIII
tetraphenylporphyrin
(TPP-Fe)
and
tetra(pentafluorophenyl)porphyrin
(TPFP-Fe),
we
showed
their
different
electrochemical
chemical
behaviors
for
ORR.
Mechanism
studies
revealed
that
FeIII-superoxo
species
TPP-Fe
can
undergo
smooth
protonation
trifluoroacetic
acid
(TFA)
electron-deficient
TPFP-Fe
cannot
be
protonated
TFA.
The
reactivity
difference
between
origin
catalytic
ORR
behaviors.
Chemical Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
AIE
luminogens
(AIEgens)
are
a
class
of
unique
fluorescent
molecules
that
exhibit
significantly
enhanced
luminescence
properties
and
excellent
photostability
in
the
aggregated
state.
Recently,
it
has
been
found
some
AIEgens
can
produce
reactive
oxygen
species,
which
means
they
may
have
potential
enzyme-like
activities
thus
termed
"AIEzymes".
Consequently,
discovery
design
novel
with
emerged
as
new
exciting
research
direction.
Additionally,
enhance
catalytic
efficiency
traditional
nanozymes
by
direct
combination,
thereby
endowing
multifunctionality.
In
this
regard,
aggregation-induced
emission
(AIE)
properties,
represents
win-win
integration,
not
only
take
full
advantage
low
cost
stability
nanozymes,
but
also
incorporate
biocompatibility
fluorescence
AIEgens.
These
synergistic
compounds
bring
about
opportunities
for
various
applications,
making
AIEzymes
interest
biomedical
research,
food
analysis,
environmental
monitoring,
especially
imaging-guided
diagnostics.
This
review
will
provide
an
overview
latest
strategies
achievements
rational
preparation
AIEzymes,
well
current
trends,
future
challenges
prospective
solutions.
We
expect
work
encourage
motivate
more
people
to
study
explore
further
promote
their
applications
fields.
Angewandte Chemie,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Abstract
Nanozymes
with
atomically
dispersed
metal
sites
(ADzymes),
especially
single‐atom
nanozymes,
have
attracted
widespread
attention
in
recent
years
due
to
their
unique
advantages
mimicking
the
active
of
natural
enzymes.
These
nanozymes
not
only
maximize
exposure
catalytic
but
also
possess
superior
activity
performance,
achieving
challenging
reactions.
position
ADzymes
as
highly
promising
candidates
field
sensing
and
biosensing.
This
review
summarizes
classification
properties
ADzymes,
systematically
highlighting
some
typical
regulation
strategies
involving
central
metal,
coordination
environment,
etc.,
achieve
catalytical
activity,
specificity,
multifunctionality.
Then,
we
present
advances
different
fields,
including
colorimetry,
fluorescence,
electrochemistry,
chemiluminescence,
photoelectrochemistry,
electrochemiluminescence.
Taking
advantage
resultant
show
great
potential
goal
sensitivity,
selectivity
accuracy
for
detection
various
targets.
Specifically,
underlying
mechanisms
terms
signal
amplification
were
discussed
detail.
Finally,
current
challenges
perspectives
on
development
advanced
are
discussed.
