OCT4 translationally promotes AKT signaling as an RNA-binding protein in stressed pluripotent stem cells
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Фев. 23, 2025
Язык: Английский
Propagating pluripotency – The conundrum of self‐renewal
BioEssays,
Год журнала:
2024,
Номер
46(12)
Опубликована: Авг. 23, 2024
Abstract
The
discovery
of
mouse
embryonic
stem
cells
in
1981
transformed
research
mammalian
developmental
biology
and
functional
genomics.
subsequent
generation
human
pluripotent
(PSCs)
the
development
molecular
reprogramming
have
opened
unheralded
avenues
for
drug
cell
replacement
therapy.
Here,
I
review
history
PSCs
from
perspective
that
long‐term
self‐renewal
is
a
product
vitro
signaling
environment,
rather
than
an
intrinsic
feature
embryos.
discuss
relationship
between
states
captured
to
stages
epiblast
embryo
suggest
key
considerations
evaluation
PSCs.
A
remaining
fundamental
challenge
determine
whether
naïve
pluripotency
can
be
propagated
broad
range
mammals
by
exploiting
common
principles
gene
regulatory
architecture.
Язык: Английский
Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee
Cell stem cell,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 1, 2025
Naive
pluripotent
stem
cells
(PSCs)
are
counterparts
of
early
epiblast
in
the
mammalian
embryo.
Mouse
and
human
naive
PSCs
differ
self-renewal
requirements
extraembryonic
lineage
potency.
Here,
we
investigated
generation
chimpanzee
PSCs.
Colonies
generated
by
resetting
or
reprogramming
failed
to
propagate.
We
discovered
that
is
enabled
inhibition
Polycomb
repressive
complex
2
(PRC2).
Expanded
show
global
transcriptome
proximity
embryo
pre-implantation
epiblast,
with
shared
expression
a
subset
pluripotency
transcription
factors.
Chimpanzee
can
transition
multilineage
competence
differentiate
into
trophectoderm
hypoblast,
forming
tri-lineage
blastoids.
They
thus
provide
higher
primate
comparative
model
for
studying
embryogenesis.
Genetic
deletions
confirm
PRC2
mediates
growth
arrest.
Further,
overcomes
roadblock
feeder-free
propagation
Therefore,
excess
deposition
chromatin
modification
H3K27me3
an
unexpected
barrier
PSC
self-renewal.
Язык: Английский
Relationship of PSC to embryos: Extending and refining capture of PSC lines from mammalian embryos
BioEssays,
Год журнала:
2024,
Номер
46(12)
Опубликована: Окт. 14, 2024
Abstract
Pluripotent
stem
cell
lines
derived
from
preimplantation
mouse
embryos
have
opened
opportunities
for
the
study
of
early
mammalian
development
and
generation
genetically
uncompromised
material
differentiation
into
specific
types.
Murine
embryonic
cells
are
highly
versatile
can
be
engineered
introduced
host
embryos,
transferred
to
recipient
females,
gestated
investigate
gene
function
at
multiple
levels
as
well
developmental
mechanisms,
including
lineage
segregation
competition.
In
this
review,
we
summarize
biomedical
motivation
driving
incremental
modification
culture
regimes
analyses
that
advanced
research
its
current
state.
Ongoing
investigation
divergent
mechanisms
processes
adopted
by
other
species,
such
agriculturally
beneficial
mammals
birds,
will
continue
enrich
knowledge
inform
strategies
future
in
vitro
models.
Язык: Английский