Relationship of PSC to embryos: Extending and refining capture of PSC lines from mammalian embryos DOI Creative Commons

Qi‐Long Ying,

Jennifer Nichols

BioEssays, Journal Year: 2024, Volume and Issue: 46(12)

Published: Oct. 14, 2024

Abstract Pluripotent stem cell lines derived from preimplantation mouse embryos have opened opportunities for the study of early mammalian development and generation genetically uncompromised material differentiation into specific types. Murine embryonic cells are highly versatile can be engineered introduced host embryos, transferred to recipient females, gestated investigate gene function at multiple levels as well developmental mechanisms, including lineage segregation competition. In this review, we summarize biomedical motivation driving incremental modification culture regimes analyses that advanced research its current state. Ongoing investigation divergent mechanisms processes adopted by other species, such agriculturally beneficial mammals birds, will continue enrich knowledge inform strategies future in vitro models.

Language: Английский

OCT4 translationally promotes AKT signaling as an RNA-binding protein in stressed pluripotent stem cells DOI Creative Commons
Wenjie Chen, Xinyu Chen, Chen Cheng

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 23, 2025

Language: Английский

Citations

1
Propagating pluripotency – The conundrum of self‐renewal DOI Creative Commons
Austin Smith

BioEssays, Journal Year: 2024, Volume and Issue: 46(12)

Published: Aug. 23, 2024

Abstract The discovery of mouse embryonic stem cells in 1981 transformed research mammalian developmental biology and functional genomics. subsequent generation human pluripotent (PSCs) the development molecular reprogramming have opened unheralded avenues for drug cell replacement therapy. Here, I review history PSCs from perspective that long‐term self‐renewal is a product vitro signaling environment, rather than an intrinsic feature embryos. discuss relationship between states captured to stages epiblast embryo suggest key considerations evaluation PSCs. A remaining fundamental challenge determine whether naïve pluripotency can be propagated broad range mammals by exploiting common principles gene regulatory architecture.

Language: Английский

Citations

4
Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee DOI Creative Commons
Tao Huang, Arthur Radley, Ayaka Yanagida

et al.

Cell stem cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Naive pluripotent stem cells (PSCs) are counterparts of early epiblast in the mammalian embryo. Mouse and human naive PSCs differ self-renewal requirements extraembryonic lineage potency. Here, we investigated generation chimpanzee PSCs. Colonies generated by resetting or reprogramming failed to propagate. We discovered that is enabled inhibition Polycomb repressive complex 2 (PRC2). Expanded show global transcriptome proximity embryo pre-implantation epiblast, with shared expression a subset pluripotency transcription factors. Chimpanzee can transition multilineage competence differentiate into trophectoderm hypoblast, forming tri-lineage blastoids. They thus provide higher primate comparative model for studying embryogenesis. Genetic deletions confirm PRC2 mediates growth arrest. Further, overcomes roadblock feeder-free propagation Therefore, excess deposition chromatin modification H3K27me3 an unexpected barrier PSC self-renewal.

Language: Английский

Citations

0
Relationship of PSC to embryos: Extending and refining capture of PSC lines from mammalian embryos DOI Creative Commons

Qi‐Long Ying,

Jennifer Nichols

BioEssays, Journal Year: 2024, Volume and Issue: 46(12)

Published: Oct. 14, 2024

Abstract Pluripotent stem cell lines derived from preimplantation mouse embryos have opened opportunities for the study of early mammalian development and generation genetically uncompromised material differentiation into specific types. Murine embryonic cells are highly versatile can be engineered introduced host embryos, transferred to recipient females, gestated investigate gene function at multiple levels as well developmental mechanisms, including lineage segregation competition. In this review, we summarize biomedical motivation driving incremental modification culture regimes analyses that advanced research its current state. Ongoing investigation divergent mechanisms processes adopted by other species, such agriculturally beneficial mammals birds, will continue enrich knowledge inform strategies future in vitro models.

Language: Английский

Citations

1