DPPH Radical Scavenging Assay

Processes, Год журнала: 2023, Номер 11(8), С. 2248 - 2248

Опубликована: Июль 26, 2023

Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals human metabolism and their deterioration during processing storage fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, has been a parallel increase use assays estimate antioxidant efficacy food systems. many bioanalytical methods that measure effect. Of these, 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay most putative, popular, commonly used method determine ability. this review, general approach DPPH radical scavenging taken. context, studies, including attempts adapt different analytes, search for highest activity values, optimize measurement, have previously performed. Therefore, it highly important introduce measures aimed at standardizing conditions activity, various reaction media suitable assay. For aim, chemical basic principles defined discussed outline. addition, study describes defines sections biological Additionally, some chemical, critical, technical details removal given. This simple which prospective compounds or herbal extracts mixed with solution absorbance measured after certain period. However, despite rapid advances instrumental techniques analysis, not undergone extreme modification. presents detailed information about in-depth review developments.

Язык: Английский

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Innovative sono-synthesis of cerium dioxide nanomaterials using mentha extract with efficient activity for cancer therapy application

Results in Engineering, Год журнала: 2024, Номер 23, С. 102720 - 102720

Опубликована: Авг. 10, 2024

Nanostructured CeO2 was fabricated through a facile sonochemical route utilizing cerium (III) nitrate hexahydrate as metal source and mentha extract novel capping agent. To our knowledge, this experimental work is the first successful endeavor for fabricating nanostructured employing by an easy eco-friendly route. The microstructure various characteristics of oxide nanostructure prepared were investigated using methods. This research examined anticancer effects green synthesized nanoparticles (CeO2-NPs) on cancer cell lines (T98 SHSY5Y) at several concentrations 0.0, 0.05, 0.1, 0.2, 0.4, 0.8 mg/ml NPs exposure time 24/48 h. obtained results presented cytotoxicity against cancerous lines. Considering CeO2-NP's cells indicated significant toxicity lines, these could be applied in medicine developing new drug formulations Additional investigations are desired to reveal other biological applications normal

Язык: Английский

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Evaluation of the in vitro antioxidant, antidiabetic and anticholinergic properties of rosmarinic acid from rosemary (Rosmarinus officinalis L.)

Biocatalysis and Agricultural Biotechnology, Год журнала: 2022, Номер 43, С. 102417 - 102417

Опубликована: Июль 6, 2022

Язык: Английский

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Assessment of hypolipidemic and anti‐inflammatory properties of walnut (Juglans regia) seed coat extract and modulates some metabolic enzymes activity in triton WR‐1339‐induced hyperlipidemia in rat kidney, liver, and heart

Journal of Molecular Recognition, Год журнала: 2022, Номер 36(3)

Опубликована: Дек. 20, 2022

Atherosclerosis and cognitive impairment are both influenced by hyperlipidemia. Due to their high margin of safety low cost, natural chemicals have recently attracted particular attention in the context treatment disease. Hence, purpose this study was investigate possible amendatory impact ethanol extract walnut (Juglans regia) seed coat (E-WSC) on some metabolic enzymes (glutathione reductase (GR), paraoxonase-1 (PON1), aldose (AR), sorbitol dehydrogenase (SDH), acetylcholinesterase (AChE), glutathione S-transferase (GST), butyrylcholinesterase (BChE)) activity liver, kidney, heart rats with Triton WR-1339-induced Rats were divided into five groups: control group, HL-Control group (Triton WR-1339 400 mg/kg, i.p administered group), E- WSC + 150 (150 mg/kg,o.d given 300 (E- o.d group) HL+ E-WSC (Group receiving 30 min prior administration i.p). In HL-Control, AR, SDH, BChE enzyme significantly increased all tissues compared control, while other studied decreased. The effects hyperlipidemia balance improved alterations investigated prevented E-WSC. As a result, promising compounds that can be used as adjuvant therapy disorders may found powder.

Язык: Английский

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Synthesis and Evaluation of Quinazolin‐4(3H)‐one Derivatives as Multitarget Metabolic Enzyme Inhibitors: A Biochemistry‐Oriented Drug Design

ChemistrySelect, Год журнала: 2023, Номер 8(25)

Опубликована: Июль 3, 2023

Abstract In this study, imines bearing quinazolin‐4(3 H )‐one were synthesized and their inhibitory properties investigated against some metabolic enzymes including Acetylcholinesterase (AChE), Butyrylcholinesterase (BChE), α‐Glycosidase (α‐Gly), human Carbonic Anhydrase I–II (hCA I–II). All compounds had strength with K i values in the range of 38.55±4.08–159.05±10.68 nM 41.04±6.73–177.12±8.06 hCA I hCA‐II, respectively comparison to standard acetazolamide (AZA) =125.15±0.78 (for hCA‐I) =148.75±0.92 hCA‐II). The showed potent activity α ‐Gly enzyme IC 50 value 0.34–2.28 (standard inhibitor acarbose (ACR): 3.18 nM). Also, these analogs 4.20±0.15–26.10±2.36 AChE 1.22±0.05–16.09±0.88 BChE tacrine (TAC) =37.62±6.86 AChE) =26.75±5.79 BChE). Additionally, molecular docking dynamics simulation study was carried out for determination ligand‐enzyme interactions. scores most active compound calculated as −7.31, −7.59, −6.66, −6.93 −7.11 kcal/mol AChE, BChE, I, II, α‐Gly, respectively.

Язык: Английский

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Comprehensive metabolic profiling of Acantholimon caryophyllaceum using LC–HRMS and evaluation of antioxidant activities, enzyme inhibition properties and molecular docking studies

South African Journal of Botany, Год журнала: 2022, Номер 151, С. 743 - 755

Опубликована: Ноя. 8, 2022

The Acantholimon taxon consists of thorny, perennial and subshrub flowering plants. Extraction various species are traditionally applied to treat liver disease diabetes roots boiled used wounds skin irritation. Here, we quantified, the radical scavenging activities ethanol (EEAC) water (WEAC) extracts caryophyllaceum Boiss (A. caryophyllaceum) against 1,1-diphenyl-2-picryl-hydrazyl (DPPH·) 2,2-azino-bis3-ethylbenzthiazoline-6-sulfonic acid (ABTS•+) as well their ability reduce Fe3+, Fe3+-TPTZ, Cu2+. antioxidant both were similar those standards in all assays. For instance, IC50 EEAC WEAC for DPPH 19.8 69.3 μg/mL, respectively. We also assessed inhibitory effects acetylcholinesterase (AChE), α-amylase α-glycosidase, which associated with Alzheimer's diabetes. extract inhibited these enzymes 1.137, 0.172 0.511 results liquid chromatography-high resolution mass spectrometry (LC–HRMS) revealed that (-)-epigallocatechin was main phenolic compound WEAC. Molecular docking analysis binding interactions between compounds gallate, (-)-epigallocatechin, fumaric acid, hyperoside, myricetin AChE, α-glycosidase enzymes.

Язык: Английский

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Determination of antioxidant, DNA protection, enzyme inhibition potential and molecular docking studies of a biomarker ursolic acid in Nepeta species

Journal of Biomolecular Structure and Dynamics, Год журнала: 2023, Номер 42(11), С. 5799 - 5816

Опубликована: Июль 2, 2023

Ursolic acid (UA), which has many biological properties such as anti-cancer, anti-inflammatory and antioxidant, regulates some pharmacological processes, been isolated from the flowers, leaves, berries fruits of plant species. In this work, UA was purified methanol-chloroform crude extract Nepeta species (N. aristata, N. baytopii, italica, trachonitica, stenantha) using a silica gel column with chloroform or ethyl acetate solvents via bioactivity-guided isolation. The most active sub-fractions were determined under bioactivities antioxidant DNA protection activities enzyme inhibitions. these fractions its structure elucidated by NMR spectroscopy techniques. highest amount found in stenantha (8.53 mg UA/g), while lowest trachonitica (1.92 UA/g). evaluated activities, inhibitions, kinetics interactions. inhibition values (IC50) α-amylase, α-glucosidase, urease, CA, tyrosinase, lipase, AChE, BChE between 5.08 181.96 µM. contrast, Ki observed 0.04 0.20 mM. addition, enzymes for enzyme-UA interactions calculated 0.38, 0.86, 0.45, 1.01, 0.23, 0.41, 0.01 2.24 µM, respectively. It is supported that can be widely used good against oxidative damage, an effective protector genetic diseases, suitable inhibitor metabolizing enzymes.Communicated Ramaswamy H. Sarma

Язык: Английский

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Synthesis of 1,3‐Disubtitituted Tetrahydropyrimidinium Salts and Determination of Their Biological Properties and Molecular Docking

ChemistrySelect, Год журнала: 2024, Номер 9(19)

Опубликована: Май 16, 2024

Abstract Several of 3,4,5,6‐tetrahydropyrimidinium salts with 1‐methyl functionalization are produced. By using techniques for 1 H‐NMR, 13 C‐NMR, and IR spectroscopy, all compounds were investigated. Additionally, these compounds’ abilities to block enzymes looked into. They had a highly effective inhibitory effect on the isoenzymes carbonic anhydrases I II, butyrylcholinesterase (BChE), acetylcholinesterase (AChE). K i values found in range 57.43±7.09–170.09±50.91 nM AChE, 7.19±0.42–69.08±2.44 BChE, 46.48±5.74–203.38±46.15 hCA I, 30.19±4.03–171.96±30.27 II. As result, 1,3‐disubtitituted tetrahydroprimidinium exhibited potent inhibition profiles toward indicated metabolic enzymes. One most important methods designing creating novel, medications treat Alzheimer's disease (AD) worldwide is synthesis discovery new AChE BChE inhibitors. The activities synthesized compared against various proteins that crystal structure (PDB ID: 4 M0E), 5NN0), 2CAB), II 3DC3), then drug properties molecules examined.

Язык: Английский

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Heterocyclic compounds as a magic bullet for diabetes mellitus: a review

RSC Advances, Год журнала: 2022, Номер 12(35), С. 22951 - 22973

Опубликована: Янв. 1, 2022

Diabetes mellitus (DM) is a major metabolic disorder due to hyperglycemia, which increasing all over the world.

Язык: Английский

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Antioxidant, Antiglaucoma, Anticholinergic, and Antidiabetic Effects of Kiwifruit (Actinidia deliciosa) Oil: Metabolite Profile Analysis Using LC-HR/MS, GC/MS and GC-FID

Life, Год журнала: 2023, Номер 13(9), С. 1939 - 1939

Опубликована: Сен. 20, 2023

Determining the antioxidant abilities and enzyme inhibition profiles of medicinally important plants their oils is great importance for a healthy life treatment some common global diseases. Kiwifruit (Actinidia deliciosa) oil was examined researched using several bioanalytical methods comprehensively first time in this research to determine its antioxidant, antiglaucoma, antidiabetic anti-Alzheimer's capabilities. Additionally, kiwifruit inhibitory effects on acetylcholinesterase (AChE), carbonic anhydrase II (CA II), α-amylase, which are linked number metabolic illnesses, were established. Furthermore, LC-HRMS analysis used assess phenolic content oil. It came light that contained 26 different compounds. According findings, abundant apigenin (74.24 mg/L oil), epigallocatechin (12.89 caryophyllene oxide luteolin (5.49 oil). In addition, GC-MS GC-FID studies ascertain quantity chemical composition essential Squalene (53.04%), linoleoyl chloride (20.28%), linoleic acid (2.67%), palmitic (1.54%) most compounds For radical scavenging activities oil, 1,1-diphenyl-2-picryl-hydrazil (DPPH•) 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•+) radicals techniques examined. These effectively demonstrated potent properties (IC50: 48.55 μg/mL DPPH•, IC50: 77.00 ABTS•+ scavenging). Also, reducing capabilities, iron (Fe3+), copper (Cu2+), Fe3+-2,4,6-tri(2-pyridyl)-S-triazine (TPTZ) studied. Moreover, showed considerable effect towards hCA 505.83 μg/mL), AChE 12.80 α-amylase 421.02 μg/mL). The results revealed use pharmaceutical procedure has very due anti-Alzheimer, antidiabetic, antiglaucoma effects.

Язык: Английский

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