VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Окт. 14, 2022

Abstract VASA, also known as DDX4, is a member of the DEAD-box proteins and an RNA binding protein with ATP-dependent helicase. The VASA gene expression, which required for human germ cell development, may lead to infertility. Immunocytochemistry immunohistochemistry were used examine expression in testis sections azoospermic patients, in-vitro in-silico models. Some studies fertile humans showed basal adluminal compartments seminiferous tubules. Our infertile luminal tubule. immunohistochemical analysis three cases different levels non-obstructive azoospermia revealed higher VASA-positive cells. For this purpose, Enrichr Shiny Gene Ontology databases pathway enrichment ontology. STRING Cytoscape online evaluation applied predict proteins' functional molecular interactions performed recognize master genes, respectively. According obtained results, main functions up-regulated downregulated genes include meiotic cycle, binding, differentiation. analyses presented seven i.e., DDX5, TNP2, DDX3Y, TDRD6, SOHL2, DDX31, SYCP3, hub involved infertility co-function protein–protein interaction. findings suggest that its interacting could help determine pathophysiology abnormalities

Язык: Английский

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Whole Exome Sequencing and In Silico Analysis of Human Sertoli in Patients with Non-Obstructive Azoospermia

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(20), С. 12570 - 12570

Опубликована: Окт. 20, 2022

Non-obstructive azoospermia (NOA) is a serious cause of male infertility. The Sertoli cell responds to androgens and takes on roles supporting spermatogenesis, which may This work aims enhance the genetic diagnosis NOA via discovery new hub genes implicated in human better assess odds successful sperm extraction according individual's genotype. Whole exome sequencing (WES) was done three patients find key involved NOA. We evaluated genome-wide transcripts (about 50,000 transcripts) by microarray between non-obstructive normal cells. analysis cases with different revealed that 32 were upregulated, expressions 113 downregulated versus case. For this purpose, Enrich Shiny GO, STRING, Cytoscape online evaluations applied predict functional molecular interactions proteins then recognize master pathways. enrichment demonstrated biological process (BP) terms "inositol lipid-mediated signaling", "positive regulation transcription RNA polymerase II", DNA-templated transcription" significantly changed upregulated differentially expressed (DEGs). BP investigation DEGs highlighted "mitotic cytokinesis", "regulation protein-containing complex assembly", "cytoskeleton-dependent "peptide metabolic process". Overrepresented function (MF) included "ubiquitin-specific protease binding", "protease "phosphatidylinositol trisphosphate phosphatase activity", "clathrin light chain binding". Interestingly, MF overexpression "ATPase inhibitor "glutathione transferase regulator activity". Our findings suggest these their interacting could help determine pathophysiologies germ abnormalities

Язык: Английский

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OCT4 protein and gene expression analysis in the differentiation of spermatogonia stem cells into neurons by immunohistochemistry, immunocytochemistry, and bioinformatics analysis

Stem Cell Reviews and Reports, Год журнала: 2023, Номер 19(6), С. 1828 - 1844

Опубликована: Апрель 29, 2023

Язык: Английский

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Clinical management, differential diagnosis, follow-up and biomarkers of infertile men with nonobstructive azoospermia

Translational Andrology and Urology, Год журнала: 2024, Номер 13(2), С. 359 - 362

Опубликована: Фев. 1, 2024

Язык: Английский

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Functions and mechanism of noncoding RNA in regulation and differentiation of male mammalian reproduction

Cell Biochemistry and Function, Год журнала: 2023, Номер 41(7), С. 767 - 778

Опубликована: Авг. 16, 2023

Abstract Noncoding RNAs (ncRNAs) are active regulators of a wide range biological and physiological processes, including the majority mammalian reproductive events. Knowledge activities ncRNAs in context reproduction will allow for more comprehensive comparative understanding male sterility fertility. In this review, we describe recent advances ncRNA‐mediated control emphasize importance several aspects reproduction, such as germ cell biogenesis organ activity. Furthermore, focus on gene expression regulatory feedback loops hormones ncRNA to better understand commitment function. Finally, study shows role failure provides suggestions further research.

Язык: Английский

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Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia

DNA and Cell Biology, Год журнала: 2023, Номер 42(10), С. 617 - 637

Опубликована: Авг. 23, 2023

Recent studies have shown that several members of the G-protein-coupled receptors (GPCR) superfamily play crucial roles in maintenance ion-water homeostasis sperm and Sertoli cells, development germ formation blood barrier, maturation sperm. The GPCR, guanyl-nucleotide exchange factor, membrane traffic protein, small GTPase genes were analyzed by microarray bioinformatics (3513 cell genes). In analyses three human cases with different nonobstructive azoospermia sperm, expression GOLGA8IP, OR2AT4, PHKA1, A2M, OR56A1, SEMA3G, LRRC17, APP, ARHGAP33, RABGEF1, NPY2R, GHRHR, LTB4R2, GRIK5, OR6K6, NAPG, OR6C65, VPS35, FPR3, ARL4A was upregulated, while MARS, SIRPG, OGFR, GPR150, LRRK1, NGEF downregulated. There an increase GBP3, TNF, TGFB3, CLTC cells NOA, whereas PAQR4, RRAGD, RAC2, SERPINB8, IRPB1, MRGPRF, RASA2, RGS2, RAP2A, RAB2B, ARL17, SERINC4, XIAP, DENND4C, ANKRA2, CSTA, STX18, SNAP23 A combined analysis Enrich Shiny Gene Ontology (GO), STRING, Cytoscape used to predict proteins' molecular interactions then recognize master pathways. Functional enrichment showed biological process (BP), regulation protein metabolic process, GTPase-mediated signal transduction significantly expressed up-/downregulated differentially (DEGs) function (MF) experiments DEGs up-/downregulated, it found GPCR activity, guanyl ribonucleotide binding, activity nucleoside-triphosphatase overexpressed. An GO findings BP MF be common DEGs. When these gene mutations been validated, they can create new antagonists or agonists are receptor-selective.

Язык: Английский

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Differential expression of ion channel coding genes in the endometrium of women experiencing recurrent implantation failures

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 27, 2024

Our study probed the differences in ion channel gene expression endometrium of women with Recurrent Implantation Failure (RIF) compared to fertile women. We analyzed relative genes coding for T-type Ca2+, ENaC, CFTR, and KCNQ1 channels endometrial samples from 20 RIF-affected 10 control women, aged 22-35, via microarray analysis quantitative real-time PCR. Additionally, we examined DNA methylation regulatory region using ChIP The bioinformatics component our research included Gene Ontology analysis, protein-protein interaction networks, signaling pathway mapping identify key biological processes pathways implicated RIF. This led discovery significant alterations RIF women's endometrium, most notably an overexpression CFTR reduced SCNN1A, SCNN1B, SCNN1G, CACNA1H, KCNQ1. A higher level KCNQ1's was also observed patients. Gene-set enrichment highlighted a presence involved transport membrane potential regulation, particularly sodium calcium complexes, which are vital cation movement across cell membranes. Genes were enriched broader transmembrane transporter underscoring their extensive role cellular homeostasis signaling. These findings suggest involvement pathology implantation failure, offering new insights into mechanisms behind possible therapeutic targets.

Язык: Английский

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Exploring the interaction between immune cells in the prostate cancer microenvironment combining weighted correlation gene network analysis and single-cell sequencing: An integrated bioinformatics analysis

Discover Oncology, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 30, 2024

Язык: Английский

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Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis

BMC Medical Genomics, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 25, 2025

Язык: Английский

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Analysis of microarray and single-cell RNA-seq identifies gene co-expression, cell–cell communication, and tumor environment associated with metabolite interconversion enzyme in prostate cancer

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 13, 2025

Prostate cancer (PCa) is the second most common malignant neoplasm in males and fifth leading cause of cancer-related mortality. Due to use prostate-specific antigen (PSA) screening improved biopsy techniques, persons identified with early-stage prostate often have a positive prognosis after comprehensive treatment. Nonetheless, latent illness that may present as an asymptomatic tumor individuals aged 20-30. The overall survival (OS) men advanced PCa significantly diminished. Consequently, there immediate want for innovative, accurate biomarkers detect early cancer. This research analyzed interaction network differentially expressed genes (DEGs) related metabolite interconversion enzymes by gene expression microarray data, single-cell RNA sequencing, oncogenes, suppressor (TSGs) utilizing bioinformatics techniques. kind analysis has not been documented prior studies. We then used dataset acquired Cancer Genome Atlas (TCGA) confirm our findings. Genes including CYP3A5, PDE8B, AOX1, BNIPL, FADS2, RRM2, ALDH3B2, GSTM2 be significant diagnosis treatment PCa. Our objective was provide new perspectives on molecular properties pathways DEGs uncover potential play crucial role genesis progression

Язык: Английский

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