Enzyme engineering for biocatalysis

Molecular Catalysis, Год журнала: 2024, Номер 555, С. 113874 - 113874

Опубликована: Янв. 31, 2024

Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes frequently lack the requisite attributes for effective organic synthesis. The inherent limitations in stability, catalytic activity, and selectivity of wild-type often hinder their suitability chemical Over past 25 years, there has been an unprecedented advancement protein tools, empowering enzymologists to customise precisely meet demands In this discussion, we delineate some most crucial techniques significance facilitating

Язык: Английский

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Novel applications of photobiocatalysts in chemical transformations

RSC Advances, Год журнала: 2024, Номер 14(4), С. 2590 - 2601

Опубликована: Янв. 1, 2024

Photocatalysis has proven to be an effective approach for the production of reactive intermediates under moderate reaction conditions.

Язык: Английский

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Photochemistry in Pharmaceutical Development: A Survey of Strategies and Approaches to Industry-wide Implementation

Organic Process Research & Development, Год журнала: 2024, Номер 28(4), С. 831 - 846

Опубликована: Март 8, 2024

We present and analyze the results of a pharmaceutical industry-wide survey strategies approaches that companies have for implementing photochemical reactions in discovery chemistry, process development, commercial manufacturing. The questions encompass types pursue why, reactors (batch flow) used, how they are characterized photochemistry, scale-up reactions, prioritize resources developing among other topics. focuses on many these topics from perspectives chemists scientists to highlight similarities differences between their specific transformations materials construction used reactors. clearly demonstrate photochemistry is viable synthetic strategy producing active ingredients (APIs), all way commercialization. While more prevalent early stage indicate leveraging successful scale-ups later stages often using flow significant knowledge resulting experiences. Nevertheless, there still gaps adopting across development very limited experience discussing with regulatory agencies. Overall, offers considerable benefits synthesizing APIs API processes, such as greenness sustainability, shortening routes, potential improved product quality.

Язык: Английский

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Artificial Photosynthases: Single-Chain Nanoparticles with Manifold Visible-Light Photocatalytic Activity for Challenging “in Water” Organic Reactions

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(21), С. 14397 - 14403

Опубликована: Апрель 19, 2024

Photocatalyzed reactions of organic substances in aqueous media are challenging transformations, often because scarce solubility substrates and catalyst deactivation. Herein, we report single-chain nanoparticles, SCNPs, capable efficiently catalyzing four different "in water" by employing visible light as the only external energy source. Specifically, decorated a high-molecular-weight copolymer, poly(OEGMA300-r-AEMA), with iridium(III) cyclometalated complex pendants at varying content amounts. The isolated functionalized copolymers demonstrated self-assembly into noncovalent, amphiphilic SCNPs water, which enabled efficient visible-light photocatalysis two unprecedentedly reported namely, [2 + 2] photocycloaddition vinyl arenes α-arylation N-arylamines. Additionally, aerobic oxidation 9-substituted anthracenes β-sulfonylation α-methylstyrene were successfully carried out media. Hence, merging metal-mediated for fabrication artificial photoenzyme-like nano-objects─i.e., photosynthases (APS)─our work broadens possibilities performing transformations via photocatalysis.

Язык: Английский

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Photobiocatalysis: A promising tool for sustainable synthesis

Chem Catalysis, Год журнала: 2024, Номер 4(7), С. 100933 - 100933

Опубликована: Фев. 29, 2024

Язык: Английский

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Divulging the potential role of wide band gap semiconductors in electro and photo catalytic water splitting for green hydrogen production

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION), Год журнала: 2025, Номер 68, С. 103 - 154

Опубликована: Янв. 1, 2025

Язык: Английский

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“Excited” Class I Aldolases: EDA Complex Mediated Photo-biocatalytic Enantioselective β-Alkylation of Enals

ACS Catalysis, Год журнала: 2025, Номер unknown, С. 2531 - 2539

Опубликована: Янв. 27, 2025

Язык: Английский

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3D printing for flow biocatalysis

RSC Sustainability, Год журнала: 2023, Номер 1(7), С. 1672 - 1685

Опубликована: Янв. 1, 2023

3D printing has been elaborated in all stages of a continuous flow biocatalytic process, from the reactor to support material for biocatalyst confinement or peripheral accessories that can establish highly controlled process.

Язык: Английский

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One-Pot Sequential Two-Step Photo-Biocatalytic Deracemization of sec-Alcohols Combining Photocatalytic Oxidation and Bioreduction

ACS Catalysis, Год журнала: 2024, Номер 14(3), С. 1808 - 1823

Опубликована: Янв. 19, 2024

Chiral alcohols are versatile building blocks and of particular interest in the asymmetric synthesis nonracemic active pharmaceutical ingredients, agrochemicals, fragrances, flavors, natural products, etc. Herein, we report on a "one-pot sequential two-step" concurrent oxidation–reduction photobiocatalytic process to synthesize enantiomerically enriched alcohols. In this regard, an efficient photocatalytic system based irradiation with 440 nm blue LEDs presence 9-fluorenone as metal-free photocatalyst molecular oxygen terminal oxidant dry DMSO hydrogen peroxide-neutralizing agent was used oxidize broad range racemic (hetero)benzylic into prochiral ketones quantitively (>99% conv.). The situ formed carbonyl compounds were subsequently converted corresponding chiral via biocatalytic transhydrogenation catalyzed by lyophilized E. coli cells overexpressing highly stereoselective stereocomplementary recombinant alcohol dehydrogenases (ADHs) originated from Rhodococcus ruber (E. coli/ADH-A) or erythropolis coli/ReADH) obtain (S)-alcohols Lactobacillus kefir coli/Lk-ADH) KRED-110 (R)-alcohols, respectively. Overall, elaborated deracemization using 9-fluorenone-O2-blue LED-DMSO-E. coli/ADH carried out semipreparative scale (0.25 mmol; 63 mM final conc. 4 mL) at room temperature yielded aryl 82–99.9% conv., up 92% isolated yield, 97–99.9% ee complementary chirality.

Язык: Английский

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Photobiocatalysis: More than just an interesting lab curiosity?

Chem Catalysis, Год журнала: 2024, Номер 4(10), С. 101077 - 101077

Опубликована: Авг. 23, 2024

Язык: Английский

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