The Science of The Total Environment, Год журнала: 2024, Номер 927, С. 172078 - 172078
Опубликована: Апрель 4, 2024
Язык: Английский
iMeta, Год журнала: 2024, Номер 3(1)
Опубликована: Янв. 3, 2024
Pig gastrointestinal tracts harbor a heterogeneous and dynamic ecosystem populated with trillions of microbes, enhancing the ability host to harvest energy from dietary carbohydrates contributing adipogenesis fatness. However, microbial community structure related mechanisms responsible for differences between fatty phenotypes lean pigs remained be comprehensively elucidated. Herein, we first found significant in composition potential functional capacity among different gut locations Jinhua distinct fatness phenotypes. Second, identified that lower exhibited higher levels short-chain acids colon, highlighting their enhanced carbohydrate fermentation capacity. Third, explored expressed carbohydrate-active enzyme (CAZyme) pigs, indicating involvement modulating fat storage. Notably,
Язык: Английский
Процитировано
33
Environment International, Год журнала: 2024, Номер 185, С. 108525 - 108525
Опубликована: Фев. 22, 2024
Deoxynivalenol (DON) is a common environmental pollutant that poses serious health risk to humans worldwide. This study was aim explore whether gut microbiota involved in DON-induced intestinal toxicity as well reveal effect of probiotics derived from protecting barrier and elucidate mechanism. We found DON caused disturbed microbiota, particularly Lactobacillus murinus (L. murinus) deficiency. enhanced M1 macrophage polarization decreased tight junction protein expression. Microbiota transplantation experiments showed transfer DON-disrupted healthy mice resulted delivery toxicity. Besides, lost its damaging on antibiotic-treated mice. Further intervention revealed L. induce conversion M2 phenotype through secreted extracellular vesicles (EVs) alleviate disruption. Mechanistically, EVs activate TLR2 promote release IL-10, which turn enhances function. Upon successful translation efficacy into clinical practice, created could be novel possible treatment strategy for disease.
Язык: Английский
Процитировано
27
Journal of Advanced Research, Год журнала: 2024, Номер unknown
Опубликована: Март 1, 2024
Diarrheic disease is a common intestinal health problem worldwide, causing great suffering to humans and animals. Precise manipulation strategies based on probiotics combat diarrheic diseases have not been fully developed.
Язык: Английский
Процитировано
16
iMeta, Год журнала: 2025, Номер unknown
Опубликована: Фев. 23, 2025
Inflammatory bowel disease (IBD) represents a significant challenge to global health, characterized by intestinal inflammation, impaired barrier function, and dysbiosis, with limited therapeutic options. In this study, we isolated novel strain of Bacillus subtilis (B. subtilis) observed promising effects in protecting against disruption the gut barrier. Our findings indicate that enhancement function is primarily attributed its metabolites. We identified metabolite, 2-hydroxy-4-methylpentanoic acid (HMP), derived from B. subtilis, significantly improved function. also show growth arrest DNA damage 45A (GADD45A) key regulator mucosal integrity, which activated HMP subsequently activates downstream Wnt/β-catenin pathway. potentially contribute development probiotics-derived metabolites or targeted "postbiotics" as therapeutics for treatment prevention IBD other diseases associated dysfunction.
Язык: Английский
Процитировано
4
iMeta, Год журнала: 2024, Номер 3(4)
Опубликована: Май 14, 2024
Abstract The duck gastrointestinal tract (GIT) harbors an abundance of microorganisms that play important role in health and production. Here, we constructed the first relatively comprehensive gut microbial gene catalog (24 million genes) 4437 metagenome‐assembled genomes using 375 GIT metagenomic samples from four different breeds across five intestinal segments under two distinct rearing conditions. We further characterized region‐specific taxonomy their assigned functions, as well temporal development maturation microbiome. Our analysis revealed similarity within microbiota foregut hindgut compartments, but distinctive taxonomic functional differences between segments. In addition, found a significant shift composition newly hatched ducks (3 days), followed by increased diversity enhanced stability growth stages (14, 42, 70 indicating develops into mature stable community host matures. Comparing impact conditions (with without water) on cecal communities bacterial capacity for lipopolysaccharide biosynthesis was significantly had free access to water, leading accumulation pathogenic bacteria antibiotic‐resistance genes. Taken together, our findings expand understanding microbiome signatures linked regional, development, ducks, which highlight poultry
Язык: Английский
Процитировано
11
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 7, 2025
Abstract Background Sow longevity and reproductivity are essential in the modern swine industry. Although many studies have focused on genetic genomic factors for selection, little is known about associations between microbiome sows with reproduction. Results In this study, we collected sequenced rectal vaginal swabs from 48 sows, nine of which completed up to four parities (U4P group), exhibiting reproductive longevity. We first identified predictors sow rectum (e.g., Akkermansia ) vagina Lactobacillus U4P group using RandomForest early breeding stage parity. Interestingly, these bacteria showed decreased predicted KEGG gene abundance involved biosynthesis amino acids. Then, tracked longitudinal changes over sows. LEfSe analysis revealed parity-associated that existed both Streptococcus Parity 1, 2, Veillonella 4). also patterns bacterial change (d 0) d 110, such as , was all parties. Furthermore, potential better performance. Finally, discovered Prevotellaceae NK3B31 total number piglets born throughout vagina. Conclusions This study highlights how reproduction within parities. The identification parity-associated, pregnancy-related, performance-correlated provides foundation targeted modulation improve animal production.
Язык: Английский
Процитировано
1
Frontiers in Microbiology, Год журнала: 2025, Номер 15
Опубликована: Янв. 17, 2025
Donkeys, as single-stomach herbivores, have a complex and diverse microbial community in their digestive tracts. The intestinal bacterial is crucial for maintaining homeostasis, well the host's overall nutrition health. However, research on donkey gut microbes relatively limited, particularly regarding colonization patterns different segments of adult donkeys. Therefore, this study examined abundance function microbiota across various sites tract (duodenum, jejunum, ileum, cecum, colon) feces healthy Dezhou male donkeys using 16S rRNA gene sequencing PICRUSt analysis. results indicate that rich diversity large population. No significant differences indices alpha were observed among donkey's duodenum, colon, feces. A Venn diagram analysis revealed presence both unique (Duodenum: 4645; Jejunum: 3586; Ileum: 4904; Cecum: 4253; Colon: 6135; Feces: 4885) shared (339) ASVs sections. principal coordinate (PCoA) (R2 = 0.2076, p < 0.007) six At phylum level, Firmicutes (63.64%), Bacteroidetes (20.72%), Verrucomicrobiota (9.16%), Patescibacteria (1.95%), Spirochaetota (1.87%), Actinobacteriota (1.13%), Proteobacteria (0.42%) dominant bacteria all samples. Wilcoxon rank-sum test proportions genera segments. Specific significantly enriched segments: Lachnospiraceae_UCG-008 Sphaerochaeta duodenum; Christensenellaceae_R-7_group Bacillus jejunum; NK4A214_group Alloprevotella ileum; UCG-005 Lactobacillus cecum; Clostridium_sensu_stricto_1 Chlamydia colon; Rikenellaceae_RC9_gut_group Prevotellaceae_UCG-004 PICRUSt2 functional prediction indicated carbohydrate metabolism, prokaryotic cellular communities, antimicrobial drug resistance, immune diseases, membrane transport, signal transduction, transcription exhibited This provided critical primary data synergistic effects between host functions. These findings can be used to assess health status, improve breeding, develop additives.
Язык: Английский
Процитировано
1
Food & Function, Год журнала: 2024, Номер 15(12), С. 6459 - 6474
Опубликована: Янв. 1, 2024
Deoxynivalenol (DON) pollution is prevalent in crops, and can induce oxidative stress intestinal injury. Hesperidin one of the major flavonoids citrus fruits that has various biological activities such as antioxidant anti-inflammatory activities. However, whether hesperidin could alleviate DON-induced injury mechanism remain unclear. Mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) have attracted attention for their crucial signaling points to regulate ER-mitochondria calcium transfer. This study aims evaluate effects on barrier, mitochondrial function, MAMs, inositol 1,4,5-triphosphate receptor (IP3R)-mitochondrial uniporter (MCU) axis intestine piglets exposed DON. Twenty-four were randomly divided into four groups a 2 × factorial arrangement 21-d experiment: Control: basal diet; group: diet + 300 mg kg
Язык: Английский
Процитировано
6
iMetaOmics., Год журнала: 2024, Номер 1(1)
Опубликована: Авг. 8, 2024
iMetaOmics is a quarterly international journal with the priority of publishing research work within scope One Health, featured well-designed omics. The also welcomes systematic integration extensive public datasets, new and meaningful perspectives, re-analysis high-impact data yielding different/valuable conclusions. microbiome plays critical roles in human [1], animal [2], plant nutrition health [3], food [4], environment [5], as well other aspects society (Figure 1). Metaomics, including metabarcoding, metagenomics, metatranscriptomics, metaproteomics, metabolomics, provide unique powerful tools to better understand taxonomy functions microbiome, biology, [6]. Aiming fill gap lacking top journals field Asia, iMeta Science Society its thousands Chinese scientist members found young but journal—iMeta (ISSN: 2770-5986; eISSN: 2770-596X). It has published more than 200 high-quality papers since 2022, original articles, reproducible methods protocols, systemic reviews peers updated novel findings, easy-to-use analytic tools, knowledge [7]. These publications include are not limited gut pertaining diseases [8], microbiota animals (e.g., chicken [9], pig [10], panda [11]), pan-cancer [12] pan-genome [13], soil associated diversity defense [14, 15], popular for metaomics analyses [16-18]. With great efforts contributions our stakeholders, like publisher, editorial board members, authors, reviewers, received first impact factor 23.7 2024, gradually accumulating broad diverse audiences readership, especially attracting explosive growth submissions recent days. To cope significantly increased submissions, we have from bimonthly journal. However, publication space maintaining high standard iMeta, still reject 90% although many them did an excellent work. Due success officially launching sister (eISSN: 2996-9514, ISSN: 2996-9506), further publish articles [19-23], [24-28], state-of-the-art methodologies [29, 30] satisfy increasing submission demand iMeta. Taking advantage same team, rejected manuscripts valuable/meaningful findings will be transferred iMetaOmics, together detailed comments reviewers. In this context, following review process shortened promote rapid publishing. Before official launch, 12 already been upon approval by authors issue. Currently, featuring reanalysis expected that establish reputation soon, attract wide range interest readers, contribute progress Human Environmental Health studies. Authors' own priority, while utilization analysis robust experimental validation can considered. Multiple evidence required support conclusion. New strategies advantage, indispensable. All used without reasonable concerns should publicly/temporally accessible reviewers editors. A video tutorial, software download, installation, operation, result displaying, highly recommended bioinformatic tools. Additionally, necessary ethics numbers, clear layouts, figures, significant expected. Chun-Lin Shi: Writing—original draft; writing—review editing. Tong Chen: Writing—review Canhui Lan: Ren-You Gan: Jun Yu: Fangqing Zhao: Yong-Xin Liu: editing; conceptualization. Zhao Yu hold position Editor-in-Chief iMetaOmics. Liu, Chen, Lan, Shi Gan Executive Editors
Язык: Английский
Процитировано
6
Fermentation, Год журнала: 2024, Номер 10(4), С. 207 - 207
Опубликована: Апрель 11, 2024
High-fiber, low-cost agricultural byproducts offer a sustainable alternative for mitigating the competition crops between humans and livestock. Pigs predominantly utilize dietary fibers through process of microbial fermentation within gut. This study explored gut microbiota capacity carbohydrate degradation in 30 individual Lantang pigs, breed indigenous to China. Through metagenomic analysis, total 671 metagenome-assembled genomes (MAGs) were assembled assigned into 14 bacterial 1 archaeal phylum, including 97 species from uncultured microbes. The phylum with highest abundance identified as Bacillota_A, Bacteroidota, Bacillota. Remarkably, investigation revealed nearly 10,000 genes implicated carbohydrates, pronounced prevalence five principal genera: Prevotella, Cryptobacteroides, Gemmiger, Vescimonas, Faecousia. Additionally, 87 distinct types carbohydrate-degrading enzymes exclusively pig. These insights not only enhance our understanding diversity specific native Chinese pig breeds but also augment body research regarding porcine fiber capabilities. implications this are twofold: it provides strategic directions optimizing feed efficiency reducing breeding costs, furnishes an expanded gene pool synthesis industrial future.
Язык: Английский
Процитировано
4