Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 14, 2024

Abstract Triple-negative breast cancer (TNBC) is a highly metastatic and heterogeneous type of with poor outcomes. Precise, non-invasive methods for diagnosis, monitoring prognosis TNBC are particularly challenging due to paucity biomarkers. Glycans on extracellular vesicles (EVs) hold the promise as valuable biomarkers, but conventional glycan analysis not feasible in clinical practice. Here, we report that lectin-based thermophoretic assay (EVLET) streamlines vibrating membrane filtration (VMF) amplification, allowing rapid, sensitive, selective cost-effective EV profiling plasma. A pilot cohort study shows signature reaches 91% accuracy detection 96% longitudinal therapeutic response. Moreover, demonstrate potential predicting progression. Our EVLET system lays foundation management by glycans.

Язык: Английский

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Single-Cell Liquid Biopsy of Lung Cancer: Ultra-Simplified Efficient Enrichment of Circulating Tumor Cells and Hand-Held Fluorometer Portable Testing

ACS Nano, Год журнала: 2024, Номер 18(6), С. 5017 - 5028

Опубликована: Фев. 2, 2024

Herein, we propose a paper-based laboratory via enzyme-free nucleic acid amplification and nanomaterial-assisted cation exchange reactions (CERs) assisted single-cell-level analysis (PLACS). This method allowed for the rapid detection of mucin 1 trace circulating tumor cells (CTCs) in peripheral blood lung cancer patients. Initially, an independently developed requiring one centrifuge, two reagents (lymphocyte separation solution erythrocyte lysate), three-step, 45 min sample pretreatment was employed. The core approach consisted competitive selective identifications: copper sulfide nanoparticles (CuS NPs) to C–Ag+–C Ag+, dual quantum dots (QDs) Cu2+ CuS NPs. To facilitate multimodal point-of-care testing (POCT), integrated visualization, test strip length reading, self-developed hand-held fluorometer readout. These methods were detectable down ag/mL concentration single-cell level. Forty-seven clinical samples assayed by fluorometer, yielding 94% (30/32) sensitivity 100% (15/15) specificity with area under curve (AUC) 0.945. Nine 15 retested respectively, AUC 0.95. All results consistent imaging folate receptor (FR)-PCR kit findings, supporting its potential early diagnosis postoperative monitoring.

Язык: Английский

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A universal electrochemical sensor for detection of nucleic acids and protein based on host-guest recognition of β-cyclodextrin polymer

Microchemical Journal, Год журнала: 2024, Номер 204, С. 110987 - 110987

Опубликована: Июнь 14, 2024

Язык: Английский

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Portable Aptasensor Based on Parallel Rolling Circle Amplification for Tumor‐Derived Exosomes Liquid Biopsy

Advanced Science, Год журнала: 2024, Номер 11(32)

Опубликована: Июнь 26, 2024

Here, a separation-free and label-free portable aptasensor is developed for rapid sensitive analysis of tumor-derived exosomes (TEXs). It integrated parallel rolling circle amplification (RCA) reaction, selective binding metal ions or small molecules to nucleic acid-specific conformations, low-cost, highly handheld fluorometer. Lung cancer, example, targeted with two typical biomarkers (mucin 1 programmed cell death ligand (PD-L1)) on its exosomes. The affinity aptamers the targets modulated amount RCA products (T-Hg

Язык: Английский

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Liquid Biopsy in the Clinical Management of Cancers

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(16), С. 8594 - 8594

Опубликована: Авг. 6, 2024

Liquid biopsy, a noninvasive diagnosis that examines circulating tumor components in body fluids, is increasingly used cancer management. An overview of relevant literature emphasizes the current state liquid biopsy applications care. Biomarkers particularly DNA (ctDNA), RNAs (ctRNA), cells (CTCs), extracellular vesicles (EVs), and other components, offer promising opportunities for early diagnosis, treatment selection, monitoring, disease assessment. The implementation precision medicine has shown significant potential various types, including lung cancer, colorectal breast prostate cancer. Advances genomic molecular technologies such as next-generation sequencing (NGS) digital polymerase chain reaction (dPCR) have expanded utility enabling detection somatic variants actionable alterations tumors. also demonstrated predicting responses, monitoring minimal residual (MRD), assessing heterogeneity. Nevertheless, standardizing techniques, interpreting results, integrating them into clinical routine remain challenges. Despite these challenges, implications management, offering dynamic approach to understanding biology guiding personalized strategies.

Язык: Английский

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Self-Assembled DNA Machine and Selective Complexation Recognition Enable Rapid Homogeneous Portable Quantification of Lung Cancer CTCs

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

In this study, we systematically investigated the interactions between Cu 2+ and various biomolecules, including double-stranded DNA, Y-shaped DNA nanospheres, double strand of hybridization chain reaction (HCR), network structure cross-linked HCR (cHCR), small molecules (PPi His), using as an illustrative example. Our research demonstrated that coordination these biomolecules not only is suitable for modulating luminescent material signals through complexation reactions with but also enhances signal intensities in materials based on chemical by increasing spatial site resistance local concentration. Building upon findings, harnessed potential amplification self-assembled nanospheres selective modulation calcein conjunction aptamer targeting mucin 1 a recognition probe. We applied approach to analysis circulating tumor cells, lung cancer cell line A549 serving representative model. assay, utilizing both fluorometer handheld detector, achieved impressive detection limits ag/ml single-cell levels was successfully validated 46 clinical samples, yielding 100% specificity 86.5% sensitivity. Consequently, our strategy has paved way more portable precise disease diagnosis.

Язык: Английский

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Bimodal In Situ Analyzer for Circular RNA in Extracellular Vesicles Combined with Machine Learning for Accurate Gastric Cancer Detection

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

Circular RNAs in extracellular vesicles (EV-circRNAs) are gaining recognition as potential biomarkers for the diagnosis of gastric cancer (GC). Most current research is focused on identifying new and their functional significance disease regulation. However, practical application EV-circRNAs early GC yet to be thoroughly explored due low accuracy analysis. In this study, a hybridization chain reaction system based rectangular DNA framework guidance constructing bimodal EV-circRNA situ analyzer (BEISA) developed. The can provide dual signal outputs fluorescence electrochemical modes, enabling self-correcting detection mechanism that significantly improves assay. It has broad range an extremely limit detection. clinical cohort BEISA used four circRNAs biomarkers, combining them with machine learning multiparametric analysis, which effectively differentiated between healthy donors patients early-stage GC. believed BEISA, conjunction technology, provides efficient, sensitive, reliable tool aiding

Язык: Английский

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CrRNA Conformation-Engineered CRISPR-Cas12a System for Robust and Ultrasensitive Nucleic Acid Detection

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Despite the widespread application of CRISPR-Cas12a system in vitro diagnostics due to its high programmability and distinctive trans-cleavage activity, susceptibility crRNA component degradation sensitivity storage working conditions poses a significant challenge improving practical efficacy these diagnostic systems. Here, we show that engineered with covalently closed circular structure (C-crRNA) can replace traditional linear form functional complexes Cas12a protein, significantly enhancing anti-interference ability while maintaining specificity. Based on this finding, crRNA-mediated CRISPR molecular (CRCD) toolkit is developed successfully integrated standard nucleic acid amplification technique detect synthesized Human Papillomavirus type 16 (HPV-16) plasmids down 10 aM levels. Furthermore, CRCD applied for ultrasensitive detection 40 HPV-16 influenza A viruses clinical samples, results consistent those from PANTHER quantitative real-time polymerase chain reaction (qRT-PCR). In conclusion, strategy introduces novel paradigm engineering program Cas12a, which has potential revolutionize use CRISPR-based diagnostics.

Язык: Английский

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Target proteins-regulated DNA nanomachine-electroactive substance complexes enable separation-free electrochemical detection of clinical exosome

Biosensors and Bioelectronics, Год журнала: 2024, Номер 256, С. 116273 - 116273

Опубликована: Апрель 14, 2024

Язык: Английский

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Ion-sensitive field effect transistor biosensors for biomarker detection: current progress and challenges

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(35), С. 8523 - 8542

Опубликована: Янв. 1, 2024

The ion-sensitive field effect transistor (ISFET) has emerged as a crucial sensor device, owing to its numerous benefits such label-free operation, miniaturization, high sensitivity, and rapid response time. Currently, ISFET technology excels in detecting ions, nucleic acids, proteins, cellular components, with widespread applications early disease screening, condition monitoring, drug analysis. Recent advancements sensing techniques, coupled breakthroughs nanomaterials microelectronics, have significantly improved performance. These developments are steering ISFETs toward promising future characterized by enhanced seamless integration, multifaceted detection capabilities. This review explores the structure operational principles of ISFETs, highlighting recent research biosensors for biomarker detection. It also examines limitations these sensors, proposes potential solutions, anticipates their trajectory. aims provide valuable reference advancing measurement.

Язык: Английский

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