Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: March 14, 2024
Abstract
Triple-negative
breast
cancer
(TNBC)
is
a
highly
metastatic
and
heterogeneous
type
of
with
poor
outcomes.
Precise,
non-invasive
methods
for
diagnosis,
monitoring
prognosis
TNBC
are
particularly
challenging
due
to
paucity
biomarkers.
Glycans
on
extracellular
vesicles
(EVs)
hold
the
promise
as
valuable
biomarkers,
but
conventional
glycan
analysis
not
feasible
in
clinical
practice.
Here,
we
report
that
lectin-based
thermophoretic
assay
(EVLET)
streamlines
vibrating
membrane
filtration
(VMF)
amplification,
allowing
rapid,
sensitive,
selective
cost-effective
EV
profiling
plasma.
A
pilot
cohort
study
shows
signature
reaches
91%
accuracy
detection
96%
longitudinal
therapeutic
response.
Moreover,
demonstrate
potential
predicting
progression.
Our
EVLET
system
lays
foundation
management
by
glycans.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(6), P. 5017 - 5028
Published: Feb. 2, 2024
Herein,
we
propose
a
paper-based
laboratory
via
enzyme-free
nucleic
acid
amplification
and
nanomaterial-assisted
cation
exchange
reactions
(CERs)
assisted
single-cell-level
analysis
(PLACS).
This
method
allowed
for
the
rapid
detection
of
mucin
1
trace
circulating
tumor
cells
(CTCs)
in
peripheral
blood
lung
cancer
patients.
Initially,
an
independently
developed
requiring
one
centrifuge,
two
reagents
(lymphocyte
separation
solution
erythrocyte
lysate),
three-step,
45
min
sample
pretreatment
was
employed.
The
core
approach
consisted
competitive
selective
identifications:
copper
sulfide
nanoparticles
(CuS
NPs)
to
C–Ag+–C
Ag+,
dual
quantum
dots
(QDs)
Cu2+
CuS
NPs.
To
facilitate
multimodal
point-of-care
testing
(POCT),
integrated
visualization,
test
strip
length
reading,
self-developed
hand-held
fluorometer
readout.
These
methods
were
detectable
down
ag/mL
concentration
single-cell
level.
Forty-seven
clinical
samples
assayed
by
fluorometer,
yielding
94%
(30/32)
sensitivity
100%
(15/15)
specificity
with
area
under
curve
(AUC)
0.945.
Nine
15
retested
respectively,
AUC
0.95.
All
results
consistent
imaging
folate
receptor
(FR)-PCR
kit
findings,
supporting
its
potential
early
diagnosis
postoperative
monitoring.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(32)
Published: June 26, 2024
Here,
a
separation-free
and
label-free
portable
aptasensor
is
developed
for
rapid
sensitive
analysis
of
tumor-derived
exosomes
(TEXs).
It
integrated
parallel
rolling
circle
amplification
(RCA)
reaction,
selective
binding
metal
ions
or
small
molecules
to
nucleic
acid-specific
conformations,
low-cost,
highly
handheld
fluorometer.
Lung
cancer,
example,
targeted
with
two
typical
biomarkers
(mucin
1
programmed
cell
death
ligand
(PD-L1))
on
its
exosomes.
The
affinity
aptamers
the
targets
modulated
amount
RCA
products
(T-Hg
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(16), P. 8594 - 8594
Published: Aug. 6, 2024
Liquid
biopsy,
a
noninvasive
diagnosis
that
examines
circulating
tumor
components
in
body
fluids,
is
increasingly
used
cancer
management.
An
overview
of
relevant
literature
emphasizes
the
current
state
liquid
biopsy
applications
care.
Biomarkers
particularly
DNA
(ctDNA),
RNAs
(ctRNA),
cells
(CTCs),
extracellular
vesicles
(EVs),
and
other
components,
offer
promising
opportunities
for
early
diagnosis,
treatment
selection,
monitoring,
disease
assessment.
The
implementation
precision
medicine
has
shown
significant
potential
various
types,
including
lung
cancer,
colorectal
breast
prostate
cancer.
Advances
genomic
molecular
technologies
such
as
next-generation
sequencing
(NGS)
digital
polymerase
chain
reaction
(dPCR)
have
expanded
utility
enabling
detection
somatic
variants
actionable
alterations
tumors.
also
demonstrated
predicting
responses,
monitoring
minimal
residual
(MRD),
assessing
heterogeneity.
Nevertheless,
standardizing
techniques,
interpreting
results,
integrating
them
into
clinical
routine
remain
challenges.
Despite
these
challenges,
implications
management,
offering
dynamic
approach
to
understanding
biology
guiding
personalized
strategies.
In
this
study,
we
systematically
investigated
the
interactions
between
Cu
2+
and
various
biomolecules,
including
double-stranded
DNA,
Y-shaped
DNA
nanospheres,
double
strand
of
hybridization
chain
reaction
(HCR),
network
structure
cross-linked
HCR
(cHCR),
small
molecules
(PPi
His),
using
as
an
illustrative
example.
Our
research
demonstrated
that
coordination
these
biomolecules
not
only
is
suitable
for
modulating
luminescent
material
signals
through
complexation
reactions
with
but
also
enhances
signal
intensities
in
materials
based
on
chemical
by
increasing
spatial
site
resistance
local
concentration.
Building
upon
findings,
harnessed
potential
amplification
self-assembled
nanospheres
selective
modulation
calcein
conjunction
aptamer
targeting
mucin
1
a
recognition
probe.
We
applied
approach
to
analysis
circulating
tumor
cells,
lung
cancer
cell
line
A549
serving
representative
model.
assay,
utilizing
both
fluorometer
handheld
detector,
achieved
impressive
detection
limits
ag/ml
single-cell
levels
was
successfully
validated
46
clinical
samples,
yielding
100%
specificity
86.5%
sensitivity.
Consequently,
our
strategy
has
paved
way
more
portable
precise
disease
diagnosis.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 17, 2025
Circular
RNAs
in
extracellular
vesicles
(EV-circRNAs)
are
gaining
recognition
as
potential
biomarkers
for
the
diagnosis
of
gastric
cancer
(GC).
Most
current
research
is
focused
on
identifying
new
and
their
functional
significance
disease
regulation.
However,
practical
application
EV-circRNAs
early
GC
yet
to
be
thoroughly
explored
due
low
accuracy
analysis.
In
this
study,
a
hybridization
chain
reaction
system
based
rectangular
DNA
framework
guidance
constructing
bimodal
EV-circRNA
situ
analyzer
(BEISA)
developed.
The
can
provide
dual
signal
outputs
fluorescence
electrochemical
modes,
enabling
self-correcting
detection
mechanism
that
significantly
improves
assay.
It
has
broad
range
an
extremely
limit
detection.
clinical
cohort
BEISA
used
four
circRNAs
biomarkers,
combining
them
with
machine
learning
multiparametric
analysis,
which
effectively
differentiated
between
healthy
donors
patients
early-stage
GC.
believed
BEISA,
conjunction
technology,
provides
efficient,
sensitive,
reliable
tool
aiding
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 6, 2025
Despite
the
widespread
application
of
CRISPR-Cas12a
system
in
vitro
diagnostics
due
to
its
high
programmability
and
distinctive
trans-cleavage
activity,
susceptibility
crRNA
component
degradation
sensitivity
storage
working
conditions
poses
a
significant
challenge
improving
practical
efficacy
these
diagnostic
systems.
Here,
we
show
that
engineered
with
covalently
closed
circular
structure
(C-crRNA)
can
replace
traditional
linear
form
functional
complexes
Cas12a
protein,
significantly
enhancing
anti-interference
ability
while
maintaining
specificity.
Based
on
this
finding,
crRNA-mediated
CRISPR
molecular
(CRCD)
toolkit
is
developed
successfully
integrated
standard
nucleic
acid
amplification
technique
detect
synthesized
Human
Papillomavirus
type
16
(HPV-16)
plasmids
down
10
aM
levels.
Furthermore,
CRCD
applied
for
ultrasensitive
detection
40
HPV-16
influenza
A
viruses
clinical
samples,
results
consistent
those
from
PANTHER
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR).
In
conclusion,
strategy
introduces
novel
paradigm
engineering
program
Cas12a,
which
has
potential
revolutionize
use
CRISPR-based
diagnostics.
Journal of Materials Chemistry B,
Journal Year:
2024,
Volume and Issue:
12(35), P. 8523 - 8542
Published: Jan. 1, 2024
The
ion-sensitive
field
effect
transistor
(ISFET)
has
emerged
as
a
crucial
sensor
device,
owing
to
its
numerous
benefits
such
label-free
operation,
miniaturization,
high
sensitivity,
and
rapid
response
time.
Currently,
ISFET
technology
excels
in
detecting
ions,
nucleic
acids,
proteins,
cellular
components,
with
widespread
applications
early
disease
screening,
condition
monitoring,
drug
analysis.
Recent
advancements
sensing
techniques,
coupled
breakthroughs
nanomaterials
microelectronics,
have
significantly
improved
performance.
These
developments
are
steering
ISFETs
toward
promising
future
characterized
by
enhanced
seamless
integration,
multifaceted
detection
capabilities.
This
review
explores
the
structure
operational
principles
of
ISFETs,
highlighting
recent
research
biosensors
for
biomarker
detection.
It
also
examines
limitations
these
sensors,
proposes
potential
solutions,
anticipates
their
trajectory.
aims
provide
valuable
reference
advancing
measurement.
