Regenerative Biomaterials,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 18, 2024
Abstract
The
detection
of
residual
nuclei
in
decellularized
extracellular
matrix
(dECM)
biomaterials
is
critical
for
ensuring
their
quality
and
biocompatibility.
However,
current
evaluation
methods
have
limitations
addressing
impurity
interference
providing
intelligent
analysis.
In
this
study,
we
utilized
four
staining
techniques—hematoxylin-eosin
staining,
acetocarmine
the
Feulgen
reaction
4’,6-diamidino-2-phenylindole
staining—to
detect
dECM
biomaterials.
Each
method
was
quantitatively
evaluated
across
multiple
parameters,
including
area,
perimeter
grayscale
values,
to
establish
a
semi-quantitative
scoring
system
nuclei.
These
quantitative
data
were
further
employed
as
learning
indicators
machine
models
designed
automatically
identify
experimental
results
demonstrated
that
no
single
alone
could
accurately
differentiate
between
impurities.
table
developed.
With
table,
accuracy
determining
whether
suspicious
point
cell
nucleus
has
reached
over
98%.
By
combining
methods,
false
positives
caused
by
contamination
eliminated.
automatic
recognition
model
trained
based
on
nuclear
parameter
features
optimal
index
after
several
iterations
training
172
epochs.
artificial
intelligence
achieved
90%
detecting
use
multidimensional
integrated
with
learning,
significantly
improved
identifying
residues
slices.
This
approach
provides
more
reliable
objective
evaluating
biomaterials,
while
also
increasing
efficiency.
Bioactive Materials,
Год журнала:
2024,
Номер
34, С. 494 - 519
Опубликована: Янв. 21, 2024
Biomaterial
choice
is
an
essential
step
during
the
development
tissue
engineering
and
regenerative
medicine
(TERM)
applications.
The
selected
biomaterial
must
present
properties
allowing
physiological-like
recapitulation
of
several
processes
that
lead
to
reestablishment
homeostatic
or
organ
function.
Biomaterials
derived
from
extracellular
matrix
(ECM)
many
such
their
use
in
field
has
been
steadily
increasing.
Considering
this
growing
importance,
it
becomes
imperative
provide
a
comprehensive
overview
ECM
biomaterials,
encompassing
sourcing,
processing,
integration
into
TERM
This
review
compiles
main
strategies
used
isolate
process
ECM-derived
biomaterials
as
well
different
techniques
for
its
characterization,
namely
biochemical
chemical,
physical,
morphological,
biological.
Lastly,
some
applications
are
explored
discussed.
Current Issues in Molecular Biology,
Год журнала:
2025,
Номер
47(4), С. 251 - 251
Опубликована: Апрель 6, 2025
Tendons
connect
animal
skeletons
to
skeletal
muscles,
playing
a
crucial
role
in
weight-bearing
and
maintaining
motor
functions.
After
decellularization,
tendon
extracellular
matrix
(tECM)
retains
the
physicochemical
characteristics
similar
those
of
native
tendons.
This
has
made
tECM
promising
biomaterial
fields
tissue
engineering
regenerative
medicine
recent
years.
paper
summarizes
origin,
structure,
ECM
components
tendons,
reviews
decellularization
methods,
discusses
advancements
research
applications
decellularized
Furthermore,
it
explores
future
development
trends
xenogeneic
materials,
aiming
provide
reference
for
fundamental
biomaterials
related
Materials Today Bio,
Год журнала:
2024,
Номер
27, С. 101125 - 101125
Опубликована: Июнь 13, 2024
Extracellular
matrices
(ECMs)
play
a
key
role
in
nerve
repair
and
are
recognized
as
the
natural
source
of
biomaterials.
In
parallel
to
extensively
studied
tissue-derived
ECMs
(ts-ECMs),
cell-derived
(cd-ECMs)
also
have
capability
partially
recapitulate
complicated
regenerative
microenvironment
native
tissues.
Notably,
cd-ECMs
can
avoid
shortcomings
ts-ECMs.
Cd-ECMs
be
prepared
by
culturing
various
cells
or
even
autologous
vitro
under
pathogen-free
conditions.
And
mild
decellularization
achieve
efficient
removal
immunogenic
components
cd-ECMs.
Moreover,
more
readily
customizable
desired
functional
properties.
These
advantages
garnered
significant
attention
for
potential
neuroregenerative
medicine.
As
promising
biomaterials,
bring
new
hope
effective
treatment
peripheral
injuries.
Herein,
this
review
comprehensively
examines
current
knowledge
about
characteristics
their
mechanisms
interaction
with
regeneration,
particular
focus
on
preparation,
engineering
optimization,
scalability
The
applications
from
distinct
cell
sources
reported
tissue
highlighted
summarized.
Furthermore,
limitations
that
should
addressed
outlooks
related
clinical
translation
put
forward
well.
Journal of Biomedical Materials Research Part A,
Год журнала:
2024,
Номер
112(9), С. 1594 - 1611
Опубликована: Март 28, 2024
Abstract
In
the
study,
we
have
shown
efficacy
of
an
indigenously
developed
redox
balancing
chitosan
gel
with
impregnated
citrate
capped
Mn
3
O
4
nanoparticles
(nanogel).
Application
nanogel
on
a
wound
preclinical
mice
model
shows
role
various
signaling
molecules
and
growth
factors,
involvement
reactive
oxygen
species
(ROS)
at
every
stage,
namely
hemostasis,
inflammation,
proliferation
leading
to
complete
maturation
for
scarless
healing.
While
in
vitro
characterization
using
SEM,
EDAX,
optical
spectroscopy
reveals
pH
regulated
buffering
capacity,
vivo
studies
Swiss
albino
involving
IL‐12,
IFN‐γ,
α‐SMA
detailed
histopathological
investigation
angiogenesis
stage
elucidate
healing
process.
Biomaterials Science,
Год журнала:
2024,
Номер
12(13), С. 3360 - 3373
Опубликована: Янв. 1, 2024
Bone
injury
is
often
associated
with
tears
in
the
periosteum
and
changes
internal
stress
microenvironment
of
periosteum.
In
this
study,
we
investigated
biological
effects
periosteal
prestress
release
on
periosteum-derived
cells
(PDCs)
potential
mechanisms
endogenous
stem
cell
recruitment.
Decellularized
natural
extracellular
matrix
(ECM)
components
was
obtained
by
a
combination
physical,
chemical,
enzymatic
decellularization.
The
decellularized
removed
immunogenicity
while
retaining
network
structure
composition
ECM.
Young's
modulus
has
no
significant
difference
between
before
after
extracted
PDCs
were
further
composited
subjected
to
20%
release.
It
found
that
proliferative
capacity
seeded
significantly
enhanced
6
h
culture
supernatant
able
promote
migration
ability
within
24
h.
Enzyme-linked
immunosorbnent
assay
(ELISA)
experiments
showed
expression
stroma-derived
factor-1α
(SDF-1α)
vascular
endothelial
growth
factor
(VEGF)
increased
3
12
release,
respectively.
Furthermore,
promoted
high
osteogenic
markers
osteocalcin
(OCN),
osteopontin
(OPN),
collagen
type
I
PDCs.
change
environment
caused
sensed
integrin
β1,
mechanoreceptor
membrane
PDCs,
which
stimulated
YAP
nucleus.
These
investigations
provided
novel
method
evaluate
importance
mechanical
stimulation
periosteum,
also
great
significance
for
design
fabrication
artificial
regulation
function.
Biomaterials Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 31, 2024
Schematic
representation
of
RHLC.
dLECMs
were
obtained
from
porcine
liver
decellularization.
HepG2,
TEC,
CAF,
and
JURKAT
cells
mixed
co-cultured
on
dLECMs.
International Materials Reviews,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
Microfluidic
organ-on-chip
systems
are
promising
platforms
for
the
development
of
biomimetic
models
that
aim
to
reconstruct
3D
architecture
and
intrinsic
functionality
native
tissues.
An
in-depth
comprehension
pivotal
role
extracellular
matrix
in
intricate
cellular
responses
has
paved
way
emergence
biologically-relevant
instructive
biomaterials
can
capture
essence
cell's
microenvironment.
The
notable
evolution
realm
toward
more
realistic
vitro
tissue
capable
recreating
synergistic
cell-extracellular
interplay
is
covered.
overview
most
recent
advances
integrating
materials
into
provided,
including
exploitation
bulk
hydrogels
as
soft
material
devices
fulfill
requirements
direct
cell-matrix
interaction.
successful
application
this
cutting-edge
technology
on
tumor
modeling
then
discussed,
highlighting
great
contribution
perfusable
microvessels
elucidate
mechanistic
events
metastatic
cascade.
This
convergence
science
with
organ-on-a-chip
envisioned
foster
understanding
behavior,
shedding
light
dynamism
interactions.
Biomaterials Research,
Год журнала:
2024,
Номер
29
Опубликована: Дек. 18, 2024
Due
to
their
exceptional
cell
compatibility,
biodegradability,
and
capacity
trigger
tissue
regeneration,
extracellular
matrix
(ECM)
materials
have
drawn
considerable
attention
in
healing
regenerative
medicine.
Interestingly,
these
undergo
continuous
degradation
release
antimicrobial
peptides
(AMPs)
while
simultaneously
promoting
thereby
exerting
a
potent
antibacterial
effect.
On
this
basis,
variety
of
basic
properties
ECM
materials,
such
as
porous
adsorption,
hydrophilic
group
crosslinking,
electrostatic
can
be
used
facilitate
the
integration
agents
through
physical
chemical
approaches
order
enhance
efficacy.
This
article
reviews
recent
advancements
study
including
function
mechanism
free-standing
ECM-based
composite
materials.
In
addition,
urgent
challenges
future
research
prospects
anti-infection
industry
are
discussed.
