Semi-quantitative scoring criteria based on multiple staining methods combined with machine learning to evaluate residual nuclei in decellularized matrix DOI Creative Commons

Meng Zhong,

Hongwei He,

Panxianzhi Ni

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 18, 2024

Abstract The detection of residual nuclei in decellularized extracellular matrix (dECM) biomaterials is critical for ensuring their quality and biocompatibility. However, current evaluation methods have limitations addressing impurity interference providing intelligent analysis. In this study, we utilized four staining techniques—hematoxylin-eosin staining, acetocarmine the Feulgen reaction 4’,6-diamidino-2-phenylindole staining—to detect dECM biomaterials. Each method was quantitatively evaluated across multiple parameters, including area, perimeter grayscale values, to establish a semi-quantitative scoring system nuclei. These quantitative data were further employed as learning indicators machine models designed automatically identify experimental results demonstrated that no single alone could accurately differentiate between impurities. table developed. With table, accuracy determining whether suspicious point cell nucleus has reached over 98%. By combining methods, false positives caused by contamination eliminated. automatic recognition model trained based on nuclear parameter features optimal index after several iterations training 172 epochs. artificial intelligence achieved 90% detecting use multidimensional integrated with learning, significantly improved identifying residues slices. This approach provides more reliable objective evaluating biomaterials, while also increasing efficiency.

Language: Английский

Extracellular matrix-derived materials for tissue engineering and regenerative medicine: A journey from isolation to characterization and application DOI Creative Commons
Helena Vilaça-Faria, Jennifer Noro, Rui L. Reis

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 34, P. 494 - 519

Published: Jan. 21, 2024

Biomaterial choice is an essential step during the development tissue engineering and regenerative medicine (TERM) applications. The selected biomaterial must present properties allowing physiological-like recapitulation of several processes that lead to reestablishment homeostatic or organ function. Biomaterials derived from extracellular matrix (ECM) many such their use in field has been steadily increasing. Considering this growing importance, it becomes imperative provide a comprehensive overview ECM biomaterials, encompassing sourcing, processing, integration into TERM This review compiles main strategies used isolate process ECM-derived biomaterials as well different techniques for its characterization, namely biochemical chemical, physical, morphological, biological. Lastly, some applications are explored discussed.

Language: Английский

Citations

31
Drug-Free Extracellular Vesicles and Spatiotemporally Controlled Release Engineering Strategy for Osteogenesis and Anti-inflammatory Niches in Rotator Cuff Regeneration DOI Creative Commons
Guoyang Zhang,

Dingyi Shi,

Yiyao Wei

et al.

Composites Part B Engineering, Journal Year: 2024, Volume and Issue: unknown, P. 111928 - 111928

Published: Oct. 1, 2024

Language: Английский

Citations

4
Research Progress on the Preparation and Application of Decellularized Tendons DOI Creative Commons
Jing Li, Mingxing Wen, Sujuan Zhang

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 251 - 251

Published: April 6, 2025

Tendons connect animal skeletons to skeletal muscles, playing a crucial role in weight-bearing and maintaining motor functions. After decellularization, tendon extracellular matrix (tECM) retains the physicochemical characteristics similar those of native tendons. This has made tECM promising biomaterial fields tissue engineering regenerative medicine recent years. paper summarizes origin, structure, ECM components tendons, reviews decellularization methods, discusses advancements research applications decellularized Furthermore, it explores future development trends xenogeneic materials, aiming provide reference for fundamental biomaterials related

Language: Английский

Citations

0
Engineering cell-derived extracellular matrix for peripheral nerve regeneration DOI Creative Commons
Yingxi Xu, Xianbo Liu, Muhammad Arslan Ahmad

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101125 - 101125

Published: June 13, 2024

Extracellular matrices (ECMs) play a key role in nerve repair and are recognized as the natural source of biomaterials. In parallel to extensively studied tissue-derived ECMs (ts-ECMs), cell-derived (cd-ECMs) also have capability partially recapitulate complicated regenerative microenvironment native tissues. Notably, cd-ECMs can avoid shortcomings ts-ECMs. Cd-ECMs be prepared by culturing various cells or even autologous vitro under pathogen-free conditions. And mild decellularization achieve efficient removal immunogenic components cd-ECMs. Moreover, more readily customizable desired functional properties. These advantages garnered significant attention for potential neuroregenerative medicine. As promising biomaterials, bring new hope effective treatment peripheral injuries. Herein, this review comprehensively examines current knowledge about characteristics their mechanisms interaction with regeneration, particular focus on preparation, engineering optimization, scalability The applications from distinct cell sources reported tissue highlighted summarized. Furthermore, limitations that should addressed outlooks related clinical translation put forward well.

Language: Английский

Citations

3
A study of scarless wound healing through programmed inflammation, proliferation and maturation using a redox balancing nanogel DOI
Monojit Das, Susmita Mondal, Ria Ghosh

et al.

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: 112(9), P. 1594 - 1611

Published: March 28, 2024

Abstract In the study, we have shown efficacy of an indigenously developed redox balancing chitosan gel with impregnated citrate capped Mn 3 O 4 nanoparticles (nanogel). Application nanogel on a wound preclinical mice model shows role various signaling molecules and growth factors, involvement reactive oxygen species (ROS) at every stage, namely hemostasis, inflammation, proliferation leading to complete maturation for scarless healing. While in vitro characterization using SEM, EDAX, optical spectroscopy reveals pH regulated buffering capacity, vivo studies Swiss albino involving IL‐12, IFN‐γ, α‐SMA detailed histopathological investigation angiogenesis stage elucidate healing process.

Language: Английский

Citations

0
Regulatory effects of stress release from decellularized periosteum on proliferation, migration, and osteogenic differentiation of periosteum-derived cells DOI

G. Dong,

Jinsong Wang, Zhongmin Chen

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(13), P. 3360 - 3373

Published: Jan. 1, 2024

Bone injury is often associated with tears in the periosteum and changes internal stress microenvironment of periosteum. In this study, we investigated biological effects periosteal prestress release on periosteum-derived cells (PDCs) potential mechanisms endogenous stem cell recruitment. Decellularized natural extracellular matrix (ECM) components was obtained by a combination physical, chemical, enzymatic decellularization. The decellularized removed immunogenicity while retaining network structure composition ECM. Young's modulus has no significant difference between before after extracted PDCs were further composited subjected to 20% release. It found that proliferative capacity seeded significantly enhanced 6 h culture supernatant able promote migration ability within 24 h. Enzyme-linked immunosorbnent assay (ELISA) experiments showed expression stroma-derived factor-1α (SDF-1α) vascular endothelial growth factor (VEGF) increased 3 12 release, respectively. Furthermore, promoted high osteogenic markers osteocalcin (OCN), osteopontin (OPN), collagen type I PDCs. change environment caused sensed integrin β1, mechanoreceptor membrane PDCs, which stimulated YAP nucleus. These investigations provided novel method evaluate importance mechanical stimulation periosteum, also great significance for design fabrication artificial regulation function.

Language: Английский

Citations

0
Decellularized extracellular matrix-based bioengineered 3D breast cancer scaffolds for personalized therapy and drug screening DOI

Teeshyo Bhattacharya,

Mamta Kumari, Kulwinder Kaur

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(36), P. 8843 - 8867

Published: Jan. 1, 2024

Bioengineered 3D breast cancer model using responsive decellularized scaffold.

Language: Английский

Citations

0
Construction of 3D tumor in vitro models with immune microenvironment exhibited similar tumor properties and biomimetic physiological functionality DOI
Yuhong Jiang, Lijuan Jin, Wenyu Liu

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Schematic representation of RHLC. dLECMs were obtained from porcine liver decellularization. HepG2, TEC, CAF, and JURKAT cells mixed co-cultured on dLECMs.

Language: Английский

Citations

0
Biomaterials meet organ-on-chips – a perspective on tumor modeling DOI
Cátia F. Monteiro, Inês A. Deus, Catarina A. Custódio

et al.

International Materials Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Microfluidic organ-on-chip systems are promising platforms for the development of biomimetic models that aim to reconstruct 3D architecture and intrinsic functionality native tissues. An in-depth comprehension pivotal role extracellular matrix in intricate cellular responses has paved way emergence biologically-relevant instructive biomaterials can capture essence cell's microenvironment. The notable evolution realm toward more realistic vitro tissue capable recreating synergistic cell-extracellular interplay is covered. overview most recent advances integrating materials into provided, including exploitation bulk hydrogels as soft material devices fulfill requirements direct cell-matrix interaction. successful application this cutting-edge technology on tumor modeling then discussed, highlighting great contribution perfusable microvessels elucidate mechanistic events metastatic cascade. This convergence science with organ-on-a-chip envisioned foster understanding behavior, shedding light dynamism interactions.

Language: Английский

Citations

0
Research Progress on Extracellular Matrix-Based Composite Materials in Antibacterial Field DOI Creative Commons
Dan Cai,

T Liu,

Wei Weng

et al.

Biomaterials Research, Journal Year: 2024, Volume and Issue: 29

Published: Dec. 18, 2024

Due to their exceptional cell compatibility, biodegradability, and capacity trigger tissue regeneration, extracellular matrix (ECM) materials have drawn considerable attention in healing regenerative medicine. Interestingly, these undergo continuous degradation release antimicrobial peptides (AMPs) while simultaneously promoting thereby exerting a potent antibacterial effect. On this basis, variety of basic properties ECM materials, such as porous adsorption, hydrophilic group crosslinking, electrostatic can be used facilitate the integration agents through physical chemical approaches order enhance efficacy. This article reviews recent advancements study including function mechanism free-standing ECM-based composite materials. In addition, urgent challenges future research prospects anti-infection industry are discussed.

Language: Английский

Citations

0