Fine mapping and candidate gene analysis of the major QTL qSW-A03 for seed weight in Brassica napus

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 17, 2025

Язык: Английский

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The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives

aBIOTECH, Год журнала: 2022, Номер 3(2), С. 146 - 161

Опубликована: Июль 2, 2022

Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important crops underwent whole-genome triplication event, hindering the development functional genomics breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex precise genome engineering, have become valuable genome-editing tools opened up new avenues biotechnology. Here, we review current progress in use CRISPR/Cas technologies with an emphasis on latest breakthroughs editing. We also summarize application to trait improvements. Finally, discuss challenges future directions these comprehensive crops. Ongoing advancement combination other achievements, will play significant role genetic improvement molecular

Язык: Английский

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Application of CRISPR/Cas9 in Rapeseed for Gene Function Research and Genetic Improvement

Agronomy, Год журнала: 2022, Номер 12(4), С. 824 - 824

Опубликована: Март 28, 2022

Brassica napus L. provides high-quality edible oil and clean energy for humans. For a long time, rapeseed breeders have tried to breed improved varieties through traditional breeding strategies. However, B. is an allotetraploid species containing many repetitive sequences. It very inefficient change traits genetic methods such as hybridization random mutagenesis due gene redundancy. Today, the burgeoning CRISPR/Cas9 technology has been applied in polyploid function research targeted improvement because of its unique advantages high efficiency simplicity. This review summarizes current reports about application system rapeseed, involving important agronomic yield, content, fatty acid composition. The status emerging precise genome editing plants several potential limitations technical bottlenecks discussed, which will provide confidence researchers technology.

Язык: Английский

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Targeted genome editing in polyploids: lessons from Brassica

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Июнь 20, 2023

CRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, increasing yield quality. However, its utilization is limited to model crops which well-annotated sequences are available. Many of dietary economic importance, such wheat, cotton, rapeseed-mustard, potato, polyploids with complex genomes. Therefore, progress these been hampered due complexity. Excellent work conducted on some species Brassica improvement through editing. Although excellent editing, polyploid crops, U's triangle species, holds numerous implications improving other crops. In this review, we summarize key examples from done discuss important considerations deploying more efficiently improvement.

Язык: Английский

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Current approaches and future potential for delivering CRISPR/Cas components in oilseeds and millets

The Nucleus, Год журнала: 2024, Номер 67(1), С. 141 - 156

Опубликована: Апрель 1, 2024

Язык: Английский

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Fine mapping and candidate gene analysis of a major locus controlling ovule abortion and seed number per silique in Brassica napus L.

Theoretical and Applied Genetics, Год журнала: 2021, Номер 134(8), С. 2517 - 2530

Опубликована: Апрель 24, 2021

Язык: Английский

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Improvement of glucosinolates by metabolic engineering in Brassica crops

aBIOTECH, Год журнала: 2021, Номер 2(3), С. 314 - 329

Опубликована: Авг. 7, 2021

Язык: Английский

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Developing Genetic Engineering Techniques for Control of Seed Size and Yield

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(21), С. 13256 - 13256

Опубликована: Окт. 31, 2022

Many signaling pathways regulate seed size through the development of endosperm and maternal tissues, which ultimately results in a range variations or weight. Seed can be determined zygotic tissues (endosperm embryo) ovules. In addition, some species such as rice, is largely by husk growth. Transcription regulator factors are responsible for enhancing cell growth ovule, resulting Phytohormones induce significant effects on entire features plants also size. Moreover, vegetative parts major source nutrients, including majority carbon nitrogen-containing molecules reproductive part to control There need increase seeds without affecting number conventional breeding programs improve grain yield. past decades, many important genetic yield have been identified studied. These constitute dynamic regulatory networks governing response environmental stimuli. this review, we summarized recent advances regarding molecular regulating Arabidopsis other crops, followed discussions strategies comprehend crops' aspects balancing

Язык: Английский

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Applications and challenges of harnessing genome editing in oilseed crops

Journal of Plant Biochemistry and Biotechnology, Год журнала: 2023, Номер 32(4), С. 751 - 772

Опубликована: Янв. 13, 2023

Язык: Английский

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CRISPR-based genome editing technology and its applications in oil crops

Oil Crop Science, Год журнала: 2021, Номер 6(3), С. 105 - 113

Опубликована: Июль 1, 2021

Oil crops, mainly comprised of soybean, rapeseed, groundnut, sunflower and etc., have provided substantial edible oil other tremendous nutrients for human beings, as well valuable biofuels associated industries. The genetic improvement significant crops and/or domesticating novel high-yielding are in urgent need to cope with the ever-increasing demand various crop products. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-based genome editing technology, born a few years ago, edits stretches DNA targeted RNA-dependent fashion. Characteristics mutagenesis easy manipulation owned by technology make it been applied many plants exhibited great potential important crops. In face growing products rapid developments CRISPR-based critical review regarding its application is badly required provide references better use this modify higher yield. paper, we briefly described summarized applications future prospects

Язык: Английский

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