Abstract
The
seeds
of
oilseed
rape
(
Brassica
napus
L.)
are
used
in
large-scale
production
one
the
most
health-promoting
plant
oils
food
industry,
as
well
for
animal
feed
and
biofuel
production.
Thus,
increasing
yield
this
crop
is
crucial
economic
ecological
importance.
However,
conventional
breeding
programs
slow,
laborious
time-consuming.
Hence,
along
with
discovery
possibility
to
apply
CRISPR/Cas
technology
edit
genomes
accelerate
process,
much
effort
has
been
put
into
applying
study
specific
genes
biosynthetic
pathways,
especially
species
many
gene
copies
such
B.
.
Here,
recent
improvements
generating
CRISPR/Cas-induced
mutations
genome,
delivering
reagents
cells,
fast-checking
efficiency
targeted
mutagenesis
reagents,
transformation
regeneration
procedures
described.
Finally,
new
applications
tools
precision
discussed,
focusing
mainly
on
verified
field.
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(3), С. 484 - 509
Опубликована: Март 1, 2024
ABSTRACT
Brassica
napus
,
commonly
known
as
rapeseed
or
canola,
is
a
major
oil
crop
contributing
over
13%
to
the
stable
supply
of
edible
vegetable
worldwide.
Identification
and
understanding
gene
functions
in
B.
genome
crucial
for
genomic
breeding.
A
group
genes
controlling
agronomic
traits
have
been
successfully
cloned
through
functional
genomics
studies
.
In
this
review,
we
present
an
overview
progress
made
including
availability
germplasm
resources,
omics
databases
genes.
Based
on
current
progress,
also
highlight
main
challenges
perspectives
field.
The
advances
contribute
better
genetic
basis
underlying
complex
will
expedite
breeding
high
quality,
resistance
yield
varieties.
Plants,
Год журнала:
2022,
Номер
11(20), С. 2740 - 2740
Опубликована: Окт. 17, 2022
The
global
demand
for
oilseeds
is
increasing
along
with
the
human
population.
family
of
Brassicaceae
crops
are
no
exception,
typically
harvested
as
a
valuable
source
oil,
rich
in
beneficial
molecules
important
health.
capacity
improving
Brassica
yield
has
steadily
risen
over
last
50
years,
major
crop
napus
(rapeseed,
canola)
production
to
~72
Gt
2020.
In
contrast,
mustard
fluctuated,
rarely
farming
efficiency.
drastic
increase
B.
largely
due
stable
cooking
oil.
Furthermore,
adoption
highly
efficient
techniques,
enhancement
programs,
breeding
integration
high-throughput
phenotyping
technology
and
establishing
underlying
genetics,
yields
have
increased
by
>450
fold
since
1978.
Yield
stability
been
improved
new
management
strategies
targeting
diseases
pests,
well
understanding
complex
interaction
environment,
phenotype
genotype.
This
review
assesses
agriculturally
oilseed
species
discusses
how
contemporary
genetic
techniques
driven
improvements.
Oil Crop Science,
Год журнала:
2023,
Номер
8(3), С. 149 - 155
Опубликована: Июль 1, 2023
Brassica
napus
(B.
napus)
is
a
globally
significant
oilseed
crop,
making
substantial
contribution
to
both
human
oil
and
livestock
feed
production.
Enhancing
seed
weight
crucial
for
improving
rapeseed
yield;
however,
only
limited
number
of
weight-related
genes
have
been
functionally
validated
in
B.
thus
far.
UBIQUITIN-SPECIFIC
PROTEASE
15
(UBP15)
belongs
the
ubiquitin
protease
pathway
plays
maternal
role
prolonging
development
Arabidopsis.
The
potential
utilization
UBP15
enhancing
yield
has
remained
unexplored
until
now.
In
this
study,
we
identified
orthologs
investigated
its
functionality
using
CRISPR-Cas9
system.
We
generated
mutant
plants
with
multiple
editing
types
targeting
Bnaubp15s
successfully
isolated
T-DNA-free
homozygous
lines
that
exhibited
edits
across
four
homologs
BnaUBP15
T2
generation
plants.
Our
preliminary
data
demonstrated
mutation
BnaUBP15s
significantly
reduced
size,
weight,
plant
height
while
noticeably
increasing
primary
branches.
These
findings
not
provide
evidence
further
elucidating
molecular
mechanism
underlying
regulation
size
by
but
also
offer
promising
novel
germplasm
architecture.
IntechOpen eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 1, 2024
Edible
oilseeds
significantly
contribute
to
human
nutrition
and
health.
However,
the
production
consumption
of
edible
are
facing
several
challenges,
such
as
limited
land
water
resources,
stress
factors,
quality
oils.
Owing
its
precision
versatility,
technology
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
(CRISPR)-Cas9
has
emerged
a
vital
tool
in
field
oilseed
research.
CRISPR-Cas9
simplifies
process,
allowing
scientists
tailor
crops
more
precisely
for
industrial
applications,
nutritional
purposes,
yield
quality,
tolerance.
In
particular,
this
is
playing
crucial
role
modifying
fatty
acid
composition
crops,
addressing
industry
demands,
eventually
promoting
sustainable
agriculture.
Interestingly,
focus
on
increasing
significant
meeting
diverse
needs
both
industries
consumers.
Taking
into
account
relevant
literature,
chapter
overviews
system,
discusses
major
insights
recent
applications
achievements
research,
addresses
challenges
proposing
solutions
CRISPR/Cas9
editing
suggests
themes,
so
far
least
explored
current
context.
