Neurobiology of the fruit fly's circadian clock

Genes Brain & Behavior, Год журнала: 2004, Номер 4(2), С. 65 - 76

Опубликована: Сен. 14, 2004

Studying the fruit fly Drosophila melanogaster has revealed mechanisms underlying circadian clock function. Rhythmic behavior could be assessed to function of several genes that generate oscillations in certain brain neurons, which finally modulate a manner. This review outlines how individual pacemaker neurons fly's control rhythm locomotor activity and eclosion.

Язык: Английский

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DN1p Circadian Neurons Coordinate Acute Light and PDF Inputs to Produce Robust Daily Behavior in Drosophila

Current Biology, Год журнала: 2010, Номер 20(7), С. 591 - 599

Опубликована: Апрель 1, 2010

Язык: Английский

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Sex- and clock-controlled expression of the neuropeptide F gene in Drosophila

Proceedings of the National Academy of Sciences, Год журнала: 2006, Номер 103(33), С. 12580 - 12585

Опубликована: Авг. 8, 2006

Drosophila neuropeptide F (NPF), a homolog of vertebrate Y, functions in feeding and coordination behavioral changes larvae modulation alcohol sensitivity adults, suggesting diverse roles for this peptide. To gain more insight into adult-specific NPF neuronal functions, we studied how npf expression is regulated the adult brain. Here, report that both sex-nonspecific male-specific manners. Our data show (ms-npf) controlled by transformer (tra)-dependent sex-determination pathway. Furthermore, fruitless, one major genes functioning downstream tra, apparently an upstream regulator ms-npf transcription. Males lacking (through tra(F)-mediated feminization) or npf-ablated male flies display significantly reduced courtship activity, function neurons to modulate fruitless-regulated sexual behavior. Interestingly, groups belongs previously defined clock-controlling dorsolateral neurons. Such absent arrhythmic Clock(Jrk) cycle(02) mutants, under dual regulation circadian sex-determining factors. Based on these data, propose also plays role clock-controlled dimorphism Drosophila.

Язык: Английский

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Circadian Control of Membrane Excitability in Drosophila melanogaster Lateral Ventral Clock Neurons

Journal of Neuroscience, Год журнала: 2008, Номер 28(25), С. 6493 - 6501

Опубликована: Июнь 18, 2008

Drosophila circadian rhythms are controlled by a neural circuit containing ∼150 clock neurons. Although much is known about mechanisms of autonomous cellular oscillation, the connection between oscillation and functional outputs that control physiological behavioral poorly understood. To address this issue, we performed whole-cell patch-clamp recordings on lateral ventral neurons (LN_vs), including large (lLN_vs) small LN_vs (sLN_vs), in situ in adult fly whole-brain explants. We found two distinct sizes action potentials (APs) >50% lLN_vs fire APs spontaneously, determined originate ipsilateral optic lobe contralateral. lLN_v resting membrane potential (RMP), spontaneous AP firing rate, resistance cyclically regulated as function time day 12 h light/dark conditions (LD). RMP becomes more hyperpolarized progresses from dawn to dusk with concomitant decrease rate resistance. From dawn, depolarized, remaining stable. In contrast, defective per⁰ null mutant excitability nearly constant LD. Over 24 darkness (DD), wild-type not regulated, although gradually slightly depolarized. sLN_v most depolarized around lights-on, substantial variability centered lights-off Our results indicate LN_v encodes via clock-dependent mechanism, likely plays critical role regulating behavior.

Язык: Английский

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Clines in clock genes: fine-tuning circadian rhythms to the environment

Trends in Genetics, Год журнала: 2008, Номер 24(3), С. 124 - 132

Опубликована: Фев. 20, 2008

Язык: Английский

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Circadian Pacemaker Neurons Change Synaptic Contacts across the Day

Current Biology, Год журнала: 2014, Номер 24(18), С. 2161 - 2167

Опубликована: Авг. 21, 2014

Язык: Английский

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The neuropeptide allatostatin C from clock-associated DN1p neurons generates the circadian rhythm for oogenesis

Proceedings of the National Academy of Sciences, Год журнала: 2021, Номер 118(4)

Опубликована: Янв. 21, 2021

Significance Metazoan species optimize the timing of reproduction to maximize fitness. To understand how biological clocks direct reproduction, we investigated neural substrates that produce oogenesis rhythms in genetically amenable model organism Drosophila melanogaster . The neuropeptide allatostatin C (AstC) is an insect counterpart vertebrate somatostatin, which suppresses gonadotropin production. A subset brain circadian pacemaker neurons produces AstC. We have uncovered these clock-associated AstC generate rhythm via insulin-producing cells and juvenile hormone. Identification a conserved pathway links female clock offers insight into molecular mechanisms reproductive timing.

Язык: Английский

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The Neuronal Circuit of the Dorsal Circadian Clock Neurons in Drosophila melanogaster

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Апрель 29, 2022

Drosophila's dorsal clock neurons (DNs) consist of four clusters (DN1as, DN1ps, DN2s, and DN3s) that largely differ in size. While the DN1as DN2s encompass only two neurons, DN1ps ∼15 DN3s comprise ∼40 per brain hemisphere. In comparison to well-characterized lateral (LNs), neuroanatomy function DNs are still not clear. Over past decade, numerous studies have addressed their role fly's circadian system, leading several sometimes divergent results. Nonetheless, these agreed important fine-tune activity under light temperature cycles play essential roles linking output from LNs downstream control sleep metabolism. Here, we used Flybow specific split-GAL4 lines, trans-Tango, recently published fly connectome (called hemibrain) describe morphology greater detail, including synaptic connections other non-clock neurons. We show some DN groups heterogenous. certain strongly connected with LNs, others mainly signal circuits clock. Among latter mushroom body central complex tubercle bulb neurosecretory cells pars intercerebralis, unidentified partners. This heterogeneity may explain conflicting results previously found about functionality. Most importantly, identify putative novel communication centers network: one fiber bundle superior protocerebrum running toward anterior optic hub posterior protocerebrum. Both invaded by might an instrumental network.

Язык: Английский

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A constitutively active cryptochrome in Drosophila melanogaster

Nature Neuroscience, Год журнала: 2004, Номер 7(8), С. 834 - 840

Опубликована: Июль 18, 2004

Язык: Английский

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Separate Sets of Cerebral Clock Neurons Are Responsible for Light and Temperature Entrainment of Drosophila Circadian Locomotor Rhythms

Journal of Biological Rhythms, Год журнала: 2007, Номер 22(2), С. 115 - 126

Опубликована: Апрель 1, 2007

The fruit fly Drosophila melanogaster shows a bimodal circadian locomotor rhythm with peaks at lights-on and before lights-off, which are regulated by multiple clocks in the brain. Even under light-dark cycles, timing of evening peak is highly dependent on temperature, starting earlier lower ambient temperature but terminating almost same time. In present study, using behavioral immunohistochemical assays, authors show that separate groups clock neurons, either light-entrainable or temperature-entrainable, form functional system driving rhythm. When subjected to light cycle combined advanced 6 h relative cycle, dorsally located neurons (DNs) lateral posterior (LPNs) shifted their phase TIMELESS expression, laterally protocerebral (LNs) basically maintained original phase. Thus, LNs seem be preferentially DNs LPNs primarily temperature-entrainable. pdf(01) mutant flies lack neuropeptide PDF ventral LNs, onset was greatly even synchronized cycles more than wild-type response 6-h shift suggesting have strong impact other cells. It seems likely 2 sets cells different entrainability coupling between them, enable keep proper relationship activity respect daily cycles.

Язык: Английский

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