bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 22, 2024
Summary
Knowing
how
neural
circuits
change
with
neuronal
plasticity
and
differ
between
individuals
is
important
to
fully
understand
behavior.
Connectomes
are
typically
assembled
using
electron
microscopy,
but
this
low
throughput
impractical
for
analyzing
or
mutations.
Here,
we
modified
the
trans
-Tango
genetic
circuit-tracing
technique
identify
neurons
synaptically
downstream
of
Drosophila
s-LNv
clock
neurons,
which
show
24hr
rhythms.
target
were
labeled
specifically
in
adult
flies
a
nuclear
reporter
gene,
facilitated
their
purification
then
single
cell
sequencing.
We
call
Tango-seq,
it
allows
transcriptomic
data
–
thus
identity
be
overlayed
on
top
anatomical
data.
found
that
s-LNvs
preferentially
make
synaptic
connections
subset
CNMa+
DN1p
these
likely
plastic
connections.
also
identified
mushroom
body
Kenyon
cells.
Tango-seq
should
useful
addition
connectomics
toolkit.
Current Biology,
Год журнала:
2019,
Номер
29(21), С. 3635 - 3646.e5
Опубликована: Окт. 24, 2019
Both
the
structure
and
amount
of
sleep
are
important
for
brain
function.
Entry
into
deep,
restorative
stages
is
time
dependent;
short
bouts
selectively
eliminate
these
states.
Fragmentation-induced
cognitive
dysfunction
a
feature
many
common
human
pathologies.
Whether
normally
regulated
independent
unknown.
Here,
we
show
that
in
Drosophila
melanogaster,
activation
subset
serotonergic
neurons
fragments
without
major
changes
total
sleep,
dramatically
reducing
long
episodes
may
correspond
to
deep
Disruption
results
learning
deficits
can
be
rescued
by
pharmacologically
or
genetically
consolidating
sleep.
We
identify
two
reciprocally
connected
sets
ellipsoid
body
form
heart
serotonin-modulated
circuit
controls
architecture.
Taken
together,
findings
define
essential
controlling
its
amount.
Animal
circadian
rhythms
persist
in
constant
darkness
and
are
driven
by
intracellular
transcription-translation
feedback
loops.
Although
these
cellular
oscillators
communicate,
isolated
mammalian
clocks
continue
to
tick
away
without
intercellular
communication.
To
investigate
issues
Drosophila,
we
assayed
behavior
as
well
molecular
within
individual
brain
clock
neurons
while
blocking
communication
the
ca.
150
neuron
network.
We
also
generated
CRISPR-mediated
neuron-specific
knockouts.
The
results
point
two
key
groups:
loss
of
both
regions
but
neither
one
alone
has
a
strong
behavioral
phenotype
darkness;
between
contributes
period
determination.
Under
dark
conditions,
region
persists
network
famous
PDF-expressing
s-LNv
however
was
strongly
dependent
on
communication,
likely
because
gene
expression
vulnerable
sLNvs
depends
neuronal
firing
or
light.
Neurobiology of Sleep and Circadian Rhythms,
Год журнала:
2025,
Номер
18, С. 100112 - 100112
Опубликована: Янв. 18, 2025
Circadian
master
clocks
in
the
brain
consist
of
multiple
neurons
that
are
organized
into
populations
with
different
morphology,
physiology,
and
neuromessenger
content
presumably
functions.
In
most
animals,
these
distributed
bilaterally,
located
close
proximity
to
visual
system,
synchronized
by
eyes
light-dark
cycles
environment.
mammals
cockroaches,
each
two
consists
a
core
region
receives
information
from
shell
which
output
projections
originate,
whereas
flies
several
other
insects,
lateral
dorsal
regions.
all
cases,
morning
evening
clock
seem
exist,
communication
between
them
neurons,
as
well
connection
across
hemispheres,
is
prerequisite
for
normal
rhythmic
function.
Phenomena
such
rhythm
splitting,
internal
desynchronization
caused
"decoupling"
hemispheres
or
decoupling
certain
within
one
hemisphere.
Since
contain
relatively
few
characterized
at
individual
level,
fly
particularly
suited
study
neurons.
Here,
we
review
organization
bilateral
brain,
focus
on
synaptic
paracrine
connections
comparison
insects
mammals.
Learning & Memory,
Год журнала:
2015,
Номер
22(9), С. 426 - 437
Опубликована: Авг. 18, 2015
Circadian
clocks
evolved
under
conditions
of
environmental
variation,
primarily
alternating
light
dark
cycles,
to
enable
organisms
anticipate
daily
events
and
coordinate
metabolic,
physiological,
behavioral
activities.
However,
modern
lifestyle
advances
in
technology
have
increased
the
percentage
individuals
working
phases
misaligned
with
natural
circadian
activity
rhythms.
Endogenous
oscillators
modulate
alertness,
acquisition
learning,
memory
formation,
recall
examples
modulation
observed
across
phyla
from
invertebrates
humans.
Cognitive
performance
are
significantly
diminished
when
occurring
out
phase
Disruptions
regulation
can
lead
impairment
formation
memories
manifestation
other
cognitive
deficits.
This
review
explores
types
interactions
through
which
clock
modulates
cognition,
highlights
recent
progress
identifying
mechanistic
between
system
processes
involved
outlines
methods
used
remediate
perturbations
reinforce
adaptation.
The Journal of Comparative Neurology,
Год журнала:
2018,
Номер
526(7), С. 1209 - 1231
Опубликована: Фев. 9, 2018
Drosophila
melanogaster
is
a
long-standing
model
organism
in
the
circadian
clock
research.
A
major
advantage
relative
small
number
of
about
150
neurons,
which
built
Drosophila.
In
our
recent
work,
we
focused
on
neuroanatomical
properties
lateral
neurons
network.
By
applying
multicolor-labeling
technique
Flybow
were
able
to
identify
anatomical
similarity
previously
described
E2
subunit
evening
oscillator
clock,
by
5th
ventrolateral
neuron
(5th
s-LNv
)
and
one
ITP
positive
dorsolateral
(LNd
).
These
two
share
same
spatial
functional
properties.
We
found
both
innervating
brain
areas
with
similar
pre-
postsynaptic
sites
brain.
Here
findings
support
their
shared
function
as
main
network
like
also
previous
studies.
second
quite
surprising
finding
addresses
large
ventral
PDF-neurons
(l-LNv
s).
could
show
that
four
hardly
distinguishable
l-LNv
s
consist
subgroups
different
innervation
patterns.
While
three
reflect
well-known
branching
pattern
reproduced
PDF
immunohistochemistry,
per
hemisphere
has
distinguished
profile
restricted
only
proximal
part
medulla-surface.
named
this
"extra"
x).
suggest
subgroups.
