Microscopy and Microanalysis,
Год журнала:
2023,
Номер
29(2), С. 802 - 815
Опубликована: Фев. 8, 2023
Pollen
morphology
of
28
taxa
belonging
to
the
genus
Sabulina
Rchb.
(Caryophyllaceae)
from
Turkey
was
examined
and
compared
using
light
microscopy
scanning
electron
microscopy.
The
current
study
aimed
describe
palynological
characteristics
assess
diagnostic
value
these
features
for
taxonomic
implications.
pollen
grains
are
radially
symmetrical,
apolar,
rarely
isopolar,
pantopolyporate
with
8-28
pores.
Their
shape
is
prolate-spheroidal
A
axes
(long
axes)
19.5
μm
B
(short
19-39.5
μm.
Exine
sculpturing
microechinate-punctate
in
all
taxa.
Principal
component
analysis
used
evaluate
significant
morphological
characters
distinguishing
taxa,
three
components
accounting
95.112%
total
variance.
Numerical
revealed
that
sizes,
pore
diameters,
numbers,
distance
between
pores,
spinule
density,
punctum
numbers
particularly
most
useful
variables
separating
results
also
display
a
partial
relationship
clusters
based
on
systematics
general
macromorphology.
Microscopy Research and Technique,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
ABSTRACT
The
Cardueae
with
about
74
genera
and
2500
species
is
one
of
the
largest
tribes
family
Asteraceae.
taxonomy
complicated
unresolved,
as
it
contains
most
diverse
genera.
main
distribution
centers
are
in
eastern
western
Mediterranean,
Irano‐Turanian
region,
central
Asia,
North
Africa.
Using
pollen
morphology
can
be
a
useful
tool
for
identification
delimitation
taxa.
Therefore,
current
study
aims
to
describe
types
tribe
based
on
features.
Furthermore,
addresses
relationships
among
palynological
features
well
morphology.
Pollen
39
distributed
28
was
examined
by
scanning
electron
microscopy.
In
present
study,
statistical
analyses
various
clustering
ordination
methods
were
provided
data.
Based
these
results,
members
nine
subtribes
showed
overlap
This
extreme
heterogeneity
taxa
could
attributed
large
number
their
high
morphological
diversity.
However,
suitable
distinguished
taxonomic
differentiation
Cardueae,
including
shape,
size,
polar
axis
equatorial
diameter
ratio
[P/E],
exine
sculpture,
spine
size.
Consequently,
six
identified
studied
Cardueae.
PLoS ONE,
Год журнала:
2021,
Номер
16(9), С. e0256808 - e0256808
Опубликована: Сен. 1, 2021
Melissopalynology
is
an
important
analytical
method
to
identify
botanical
origin
of
honey.
Pollen
grain
recognition
commonly
performed
by
visual
inspection
a
trained
person.
An
alternative
for
automated
pollen
analysis
based
on
the
image
technique.
Image
transfers
information
mathematical
descriptions.
In
this
work,
suitability
three
microscopic
techniques
automatic
grains
was
studied.
2D
and
3D
morphological
characteristics,
textural
colour
features,
extended
depth
focus
characteristics
were
used
discrimination.
study,
7
taxa
total
2482
evaluated.
The
highest
correct
classification
rate
93.05%
achieved
using
phase
contrast
microscopy,
followed
dark
field
microscopy
reaching
91.02%,
finally
light
88.88%.
most
significant
discriminant
(2D
3D)
characteristics.
Our
results
confirm
potential
discriminate
in
This
work
provides
basis
further
research
where
dataset
will
be
increased,
new
descriptors
Diversity,
Год журнала:
2022,
Номер
14(9), С. 705 - 705
Опубликована: Авг. 26, 2022
In
this
research,
the
pollen
morphology
of
Rosa
sericea
complex
has
been
studied
by
Scanning
Electron
Microscope
(SEM).
The
present
study
aimed
to
provide
baseline
information
for
taxonomic
identification
R.
taxa.
sculpture,
outline,
and
aperture
show
variability
in
includes
taxonomically
challenging
species
with
undistinguishable
boundaries.
Identifying
taxon
boundaries
closely
related
is
fundamental
investigating
evolution
biodiversity.
Taxa
circumscription
insufficient,
unresolved,
a
prerequisite
comprehensive
systematic
revisions.
To
resolve
these
issues,
we
have
attempted
identify
some
important
diagnostic
characters
complex.
This
research
different
qualitative
quantitative
characteristics
pollen.
included
polar
equatorial
view,
apertures,
exine,
size
category,
polarity
contrast,
features
include
diameter,
P/E,
colpi
length
width,
distance
between
colpi.
Pollen
mono
tricolporate.
morphological
here
very
few
details
which
separate
into
distinct
Some
offer
delimitation
including
apertures.
Microscopy Research and Technique,
Год журнала:
2021,
Номер
85(2), С. 570 - 590
Опубликована: Сен. 18, 2021
This
study
aimed
to
characterize
the
palynological
morphology
of
melliferous
species
family
Asteraceae
belonged
seven
tribes
which
were
categorized
into
15
genera
and
studied
under
light
scanning
electron
microscopy.
The
pollen
grains
acetolyzed,
measured,
described
qualitatively.
Quantitative
data
analyzed
by
descriptive
multivariate
statistical
analysis.
considering
11
quantitative
characteristics.
revealed
that
are
circular,
triangular,
angular,
semi-angular,
quadrangular,
pentagonal
hexagonal
amb,
3-colporate
or
3-colpate.
size
is
variable
among
species,
ranged
from
56.1
23.2
μm.
Three
types:
oblate
spheroidal
(eight
species),
prolate
(six
suboblate
(two
species)
observed.
surface
pattern
exine
varies
echinoperforate,
echinate,
echinate
microperforate,
fenestrate,
echinoperforate
reticulate,
microreticulate,
perforate,
tectate,
scabrate.
Exine
thickness
was
calculated
minimum
in
Cichorium
intybus
(2.47
μm)
maximum
Taraxacum
campylodes
(5.15
μm).
A
key
bee
floral
based
on
morphological
features
grains,
also
provided.
palynomorphological
characters
here
can
be
potentially
used
correctly
identify
Asteraceous
honeybee
most
commonly
plants
visited
honeybees
area.
Microscopy Research and Technique,
Год журнала:
2021,
Номер
84(7), С. 1451 - 1461
Опубликована: Фев. 13, 2021
Abstract
Although
considerable
progress
has
recently
been
made
in
the
taxonomic
study
of
Asparagaceae,
but
Polygonatum
remains
one
most
poorly
investigated
genus.
Therefore,
present
was
designed
with
aim,
to
document
pollen
micromorphology
closely
related
taxa
and
its
significance.
Pollen
micro‐morphological
features
were
observed
using
scanning
electron
microscopy—SEM).
The
grains
are
monad,
navicular,
monocolpate,
radially
symmetrical.
A
significant
variation
exine
sculpturing.
Moreover,
have
perforate
while
some
them
mixed
psilate
perforate,
microreticulate,
sometimes
scabrate,
gemmate
baculate.
Hence,
this
documented
morphology
strengthens
identification
genus
based
on
characters,
which
helps
can
be
used
as
an
additional
tool
for
correct
discrimination
species
at
generic
level.
Microscopy Research and Technique,
Год журнала:
2022,
Номер
85(7), С. 2361 - 2380
Опубликована: Март 5, 2022
This
research
aimed
to
explore
the
melliferous
bee
flora
pollen
from
arid
zones
of
District
Attock,
Islamabad
Capital
and
Rawalpindi.
Morpho-palynological
features
has
provided
key
information
for
classification
help
explain
taxonomy
several
genera
species.
Therefore,
in
this
work,
we
examined
morphological
characters
potentially
be
used
species
delimitation
flora.
A
total
18
floral
species,
classified
into
12
different
families
were
collected,
pressed,
identified
then
acetolyized
observe
grains
under
microscopy.
Bee
can
described
by
small,
medium
large
sized
morphotypes
presenting
five
types
shapes,
prolate
spheroidal
(seven
species),
sub-prolate
(six
oblate
(three
(one
each)
was
examined.
The
observed
polar
equatorial
diameter
found
maximum
Zea
mays
73.5
μm
Pelargonium
inquinans
66.75
respectively.
Pollen
six
namely:
tricolporate,
3-zonocolporate,
monoportae,
tetracolporate,
tetraporate
mononsulcate.
Whereas
highest
value
colpi
length
measured
Grewia
tenax
(24.55
μm).
Exine
surface
nature
echinate
psilate
scabrate.
thickness
noted
Verbena
tenuisecta
(8.40
μm)
minimum
Citrus
macrocarpa
(0.4
considered
difficult
identify
based
on
other
traits
successfully
distinguished
using
quantitative
qualitative
traits,
confirming
importance
morphology
diagnose
correctly
honeybee
RESEARCH
HIGHLIGHTS:
diversity
among
Highly
variations
via
microscopy
micromorphology.
Palyno-morph
taxonomic
identification
Throughout
more
than
100
years
of
its
history
palynology
has
become
an
independent
discipline
that
is
being
applied
in
various
fields
from
palaeoecology,
aerobiology,
forensic
sciences
to
taxonomy.
Bibliometric
analysis
allows
distinguish
different
phases
the
evolution
palynology.
From
initial
phase,
when
first
pioneer
results
were
released,
through
building
phase
potential
pollen
been
expanding
into
new
research
areas,
mature
which
becomes
a
basic
method
with
worldwide
recognition
many
scientific
fields.
However,
scientometric
palynological
documents
suggests
second
decade
20th
century,
increasing
trend
number
published
stopped.
This
tendency
observed
most
journals
publishing
content.
may
suggest
reached
climax.
Moreover,
last
couple
show
slow
but
constant
drop
documents.
During
this
period
also
decrease
mean
citation
per
document
and
year
observed.
poses
question
–
does
issue
reflect
only
state
or
wider
phenomenon
touching
other
related
as
for
example
palaeoecology?
On
hand,
bibliometric
points
out
some
positive
aspects
such
increase
international
co-authorship
co-authors
indicates
development
specialization
discipline.