Silencing of the plant‐derived horizontally transferred gene BtSC5DL effectively controls Bemisia tabaci MED

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

Abstract BACKGROUND The whitefly Bemisia tabaci is a notorious agricultural pest known for its ability to cause significant crop damage through direct feeding and virus transmission. Its remarkable adaptability reproductive capacity are linked acquire integrate horizontally transferred genes (HTGs) into genome. These HTGs increase the physiological metabolic capacities of this pest, including cholesterol synthesis, which critical survival success. Among these genes, we identified plant‐derived B. Δ7‐sterol C5‐desaturase‐like gene ( BtSC5DL ), plays pivotal role in metabolism biology. RESULTS In study, cloned from Mediterranean (MED). Bioinformatics molecular analyses revealed that was plants millions years ago now stably expressed species. Silencing dsRNA resulted reductions egg production content MED. Furthermore, virus‐induced silencing (VIGS) experiments confirmed long‐term suppression had notable control populations. CONCLUSION Our results demonstrate crucial biosynthesis MED suggest acquisition significantly enhances findings provide theoretical basis development RNA interference (RNAi)‐based strategies targeting , offering potential new approach effective management populations settings. © 2025 Society Chemical Industry.

Язык: Английский

---

RNA interference in insects: the link between antiviral defense and pest control

Insect Science, Год журнала: 2023, Номер 31(1), С. 2 - 12

Опубликована: Май 10, 2023

RNA interference (RNAi) is a form of gene silencing triggered by double-stranded (dsRNA) that operates in all eukaryotic cells. RNAi has been widely investigated insects to determine the underlying molecular mechanism, investigate its role systemic antiviral defense, and develop strategies for pest control. When insect cells are infected viruses, viral dsRNA signatures trigger local response block replication generate virus-derived DNA confers immunity. RNAi-based control involves application exogenous targeting genes essential development or survival, but efficacy this approach limited potency many pests through combination rapid degradation, inefficient uptake/processing, ineffective machinery. This could be addressed screening evaluation, focusing on design off-target management, as well production delivery. review summarizes recent progress defense strategy insects, addressing gaps between our fundamental understanding mechanism exploitation strategies.

Язык: Английский

---

Insecticide Resistance and Its Management in Two Invasive Cryptic Species of Bemisia tabaci in China

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(7), С. 6048 - 6048

Опубликована: Март 23, 2023

The sweet potato whitefly Bemisia tabaci is a major agricultural pest with wide host range throughout the world. species designation for B. includes numerous distinct cryptic or biotypes. Two invasive biotypes, MEAM1 (B) and MED (Q), were found in China at end of 20th century beginning 21st century. (Q) show higher pesticide resistance levels than native strains, vary changes insecticide selection pressure. Recent studies have revealed metabolic mechanisms target site mutations strains that render them resistant to insecticides uncovered frequency these resistance-related populations China. Novel control agents, such as RNA-based pesticides nano-pesticides, achieved effective effects laboratory are expected be applied field future. In this review, we discuss developed by since their invasion into We also provide suggestions ecologically sound efficient control.

Язык: Английский

---

Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Сен. 6, 2024

Язык: Английский

---

Identification and RNAi‐based function analysis of trehalase family genes in Frankliniella occidentalis (Pergande)

Pest Management Science, Год журнала: 2024, Номер 80(6), С. 2839 - 2850

Опубликована: Фев. 7, 2024

Abstract BACKGROUND Insects utilize trehalases (TREs) to regulate energy metabolism and chitin biosynthesis, which are essential for their growth, development, reproduction. TREs can therefore be used as potential targets future insecticide development. However, the roles of in Frankliniella occidentalis (Pergande), a serious widespread agricultural pest, remain unclear. RESULTS Three TRE genes were identified F. cloned, functions then investigated via feeding RNA interference (RNAi) virus‐induced gene silencing (VIGS) assays. The results showed that FoTRE1‐1 or FoTRE1‐2 significantly decreased expression levels FoGFAT , FoPGM FoUAP FoCHS members biosynthesis pathway. Silencing FoTRE2 down‐regulated FoPFK FoPK These changes resulted 2‐fold decreases glucose glycogen content, increases trehalose 1.5‐ 2.0‐fold chitinase activity. Furthermore, knocking down deformed nymphs pupae result hindered molting. VIGS assay three FoTREs revealed caused shortened ovarioles, reduced egg‐laying hatching rates. CONCLUSION suggest play important growth development while its could candidate RNAi‐based management control this destructive pest. © 2024 Society Chemical Industry.

Язык: Английский

---

Plant resistance against whitefly and its engineering

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Авг. 30, 2023

Plants face constant threats from insect herbivores, which limit plant distribution and abundance in nature crop productivity agricultural ecosystems. In recent decades, the whitefly Bemisia tabaci , a group of phloem-feeding insects, has emerged as pests global significance. this article, we summarize current knowledge on defenses against approaches to engineer resistance whitefly. Physically, plants deploy trichome acylsugar-based strategies restrain nutrient extraction by Chemically, toxic secondary metabolites such terpenoids confer plants. Moreover, jasmonate (JA) signaling pathway seems be major regulator many We next review advances interfering with whitefly-plant interface engineering using conventional biotechnology-based breeding. These breeding programs have yielded lines high whitefly, hold promises for control field. Finally, conclude an outlook several issues particular relevance

Язык: Английский

---

A Horizontally Transferred Plant Fatty Acid Desaturase Gene Steers Whitefly Reproduction

Advanced Science, Год журнала: 2023, Номер 11(10)

Опубликована: Дек. 25, 2023

Abstract Polyunsaturated fatty acids (PUFAs) are essential nutrients for all living organisms. PUFA synthesis is mediated by Δ12 desaturases in plants and microorganisms, whereas animals usually obtain PUFAs through their diet. The whitefly Bemisia tabaci an extremely polyphagous agricultural pest that feeds on phloem sap of many do not always provide them with sufficient PUFAs. Here, a plant‐derived desaturase gene family BtFAD2 characterized B. it shows the BtFAD2‐9 enables to synthesize PUFAs, thereby significantly enhancing its fecundity. role reproduction further confirmed transferring Drosophila melanogaster , which also increases fruit fly's reproduction. These findings reveal extraordinary evolutionary scenario whereby phytophagous insect acquired plant genes nutrients, lessening nutritional dependency allowing feed reproduce host plants.

Язык: Английский

---

Knockdown of the Expression of Two Trehalase Genes with RNAi Disrupts the Trehalose and Chitin Metabolism Pathways in the Oriental Armyworm, Mythimna separata

Insects, Год журнала: 2024, Номер 15(3), С. 142 - 142

Опубликована: Фев. 21, 2024

Trehalose is an important carbohydrate substance in insect hemolymph. Chitin the main component of cuticle and peritrophic matrix insects. Trehalase (Tre) catalyzes decomposition trehalose. Few studies trehalase lepidopteran insects have been conducted. Here, functions soluble Tre (Tre1) membrane-bound (Tre2) growth development Mythimna separata were investigated. We cloned identified Tre1 Tre2 cDNA sequences M. separata. Analysis expression revealed that MsTre1 MsTre2 highly expressed midgut integument, respectively. The was highest pupal stage. used RNA interference (RNAi) to inhibit larvae. Injection dsMsTre1 or dsMsTre2 resulted abnormal phenotypes impeded normal molting. Silencing significant changes genes trehalose chitin metabolism pathways, significantly increased glycogen content, decreased activity, glucose content integument. slowed larval molting, new thinner. These results indicate RNAi may be useful for control strategies against

Язык: Английский

---

Plant Recombinant Gene Technology for Pest Control in the Twenty-First Century: From Simple Transgenesis to CRISPR/Cas

Опубликована: Янв. 1, 2024

The current progress in genetic technologies (methodologies) allows to create crops with the modified genome counter different pest species. Genome editing is most popular and efficient approach increase crop defense against harmful insects, mites, nematodes. During first decades of twenty-first century, methodology control using plant recombinant has evolved significantly. Toxins bacteria Bacillus thuringiensis (Bt) were heterologous protective compounds introduced into plants, until now, these remain main source transgenes for control. Heterologous expression proteins toxic pests, such as lectins protease inhibitors, was another strategy increasing resistance plants. RNA interference genes achieved through plants recently become studied area protection. CRISPR/Cas-based modification systems modern gene manipulation allow knock out genes, targeted variation, include precise insertion/deletion single nucleotide changes. All approaches are focused on targeting some physiological, biochemical, behavioral pests. This chapter summarizes improvement protection We extensively reviewed sum research articles published between 2000 2022 reporting that successful transgenic events proved be a wide range

Язык: Английский

---

Silencing of catalase reduces unfavorable low‐temperature tolerance capacity in whiteflies

Pest Management Science, Год журнала: 2024, Номер 80(7), С. 3116 - 3125

Опубликована: Фев. 9, 2024

Abstract BACKGROUND Temperature is a primary factor that determines the eco‐geographical distribution and population development of invasive insects. stress leads to various negative effects, including excess reactive oxygen species (ROS), catalase (CAT) key enzyme against ROS in antioxidant pathway. The whitefly Bemisia tabaci MED typical pest causes damage worldwide. Our previous studies have shown CAT promotes adaptation high temperature by eliminating ROS. However, mechanism underlying low‐temperature whiteflies still unknown. RESULTS In this study, we investigated role tolerance B. analyzing its survival rate, reproduction, levels at 25 °C (as control, suitable temperature), 20 (moderately decreased 4 (severely temperature). Silencing BtCAT1 , BtCAT2, or BtCAT3 reduced viability under short‐term severely (4 °C), which manifested as decreases fecundity accompanied significant increases levels. Moreover, even moderately (20 silencing led low rates adults. CONCLUSION BtCATs significantly increased sensitivity temperatures. likely more essential than other for whiteflies. © 2024 Society Chemical Industry.

Язык: Английский

---